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Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat.
Paar, Jack; Willis, Jessica R; Sette, Lisa; Wood, Stephanie A; Bogomolni, Andrea; Dulac, Monique; Sivaganesan, Mano; Shanks, Orin C.
Afiliación
  • Paar J; U.S. Environmental Protection Agency, New England Regional Laboratory, North Chelmsford, MA 01863, USA.
  • Willis JR; U.S. Environmental Protection Agency, Office of Research and Development, Center for Environmental Measurement and Modeling, Cincinnati, OH 45268, USA.
  • Sette L; Center for Coastal Studies, 5 Holway Avenue, Provincetown, MA 02657, USA.
  • Wood SA; University of Massachusetts, Boston, Biology Department, 100 Morrissey Blvd., Boston, MA 02125, USA.
  • Bogomolni A; Massachusetts Maritime Academy, Marine Science, Safety and Environmental Protection, 101 Academy Drive, Buzzards Bay, MA 02532, USA.
  • Dulac M; U.S. Environmental Protection Agency, New England Regional Laboratory, North Chelmsford, MA 01863, USA.
  • Sivaganesan M; U.S. Environmental Protection Agency, Office of Research and Development, Center for Environmental Measurement and Modeling, Cincinnati, OH 45268, USA.
  • Shanks OC; U.S. Environmental Protection Agency, Office of Research and Development, Center for Environmental Measurement and Modeling, Cincinnati, OH 45268, USA. Electronic address: shanks.orin@epa.gov.
Sci Total Environ ; 934: 173220, 2024 Jul 15.
Article en En | MEDLINE | ID: mdl-38761521
ABSTRACT
The number of gray seals (Halichoerus grypus) observed along the United States Northwest Atlantic region has been increasing for decades. These colonial animals often haul-out on beaches seasonally in numbers ranging from a few individuals to several thousands. While these larger aggregations are an important part of gray seal behavior, there is public concern that haul-outs could lead to large amounts of fecal waste in recreational areas, potentially resulting in beach closures. Yet, data to confirm whether these animals contribute to beach closures is lacking and minimal information is available on the occurrence of key water quality monitoring genetic markers in gray seal scat. This study evaluates the concentration of E. coli (EC23S857), enterococci (Entero1a), and fecal Bacteroidetes (GenBac3) as well as six fecal source identification genetic markers (HF183/BacR287, HumM2, CPQ_056, Rum2Bac, DG3, and GFD) measured by qPCR in 48 wild gray seal scat samples collected from two haul-out areas in Cape Cod (Massachusetts, U.S.A.). Findings indicate that FIB genetic markers are shed in gray seal scat at significantly different concentrations with the Entero1a genetic marker exhibiting the lowest average concentration (-0.73 log10 estimated mean copies per nanogram of DNA). In addition, systematic testing of scat samples demonstrated that qPCR assays targeting host-associated genetic markers indicative of human, ruminant, and canine fecal pollution sources remain highly specific in waters frequented by gray seals (>97 % specificity).
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Calidad del Agua / Monitoreo del Ambiente / Phocidae / Heces Límite: Animals Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Calidad del Agua / Monitoreo del Ambiente / Phocidae / Heces Límite: Animals Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos