Yanghanlia caeni gen. nov., sp. nov., a novel taxon within the family Alcaligenaceae isolated from sludge of a pesticide-manufacturing factory.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
en En
| MEDLINE
| ID: mdl-38767617
ABSTRACT
A Gram-stain-negative bacterium, designated LG-2T, was isolated from sludge collected at a pesticide-manufacturing factory in Jiangsu Province, PR China. Cells of strain LG-2T were strictly aerobic, non-motile and spherical. Growth was observed at 15-42â°C (optimum, 30â°C), pH 6.0-9.0 (optimum, pH 7.0) and 0-3.0â% (w/v) NaCl (optimum, 1.0â%). LG-2T showed 95.5-96.9â% 16S rRNA sequence similarity to type strains in the genera Pusillimonas, Bordetella, Parapusillimonas, Candidimonas and Paracandidimonas of the family Alcaligenaceae. The phylogenomic tree indicated that strain LG-2T was clustered in the family Alcaligenaceae and formed a clade with Paracandidimonas soli IMT-305T, while the phylogenetic trees based on 16S rRNA gene sequences indicated that strain LG-2T formed a distinct clade within the family Alcaligenaceae. The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between LG-2T and its closely related type strains in the genera Pusillimonas, Bordetella, Parapusillimonas, Candidimonas and Paracandidimonas were 70.8-75.3, 18.9-23.7 and 59.6â%-69.3â%, respectively. The major cellular fatty acids were C16ââ0, C17ââ0 cyclo, summed feature 3 (C16ââ1 ω7c and/or C16ââ1 ω6c), summed feature 8 (C18ââ1 ω7c and/or C18ââ1 ω6c) and summed feature 2 (C12ââ0 aldehyde and/or unknown 10.928). The predominant menaquinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, three aminolipids and nine unknown polar lipids. The genome size of strain LG-2T was 3.2 Mb and the DNA G+C content was 63.4 mol%. On the basis of the phenotypic, phylogenetic and genomic results from this study, strain LG-2T represents a novel species of a new genus in the family Alcaligenaceae, for which the name Yanghanlia caeni gen. nov., sp. nov. is proposed, with strain LG-2T (=KCTC 8084T= CCTCC AB 2023123T) as the type strain.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Filogenia
/
Aguas del Alcantarillado
/
Composición de Base
/
ADN Bacteriano
/
ARN Ribosómico 16S
/
Técnicas de Tipificación Bacteriana
/
Análisis de Secuencia de ADN
/
Alcaligenaceae
/
Ácidos Grasos
/
Hibridación de Ácido Nucleico
País/Región como asunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2024
Tipo del documento:
Article