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Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation.
Gökhan, Aylin; Çavusoglu, Türker; Kiliç, Kubilay Dogan; Sirin, Cansin; Tomruk, Canberk; Yigittürk, Gürkan; Erbas, Oytun; Yildirim Sözmen, Eser; Baka, Meral.
Afiliación
  • Gökhan A; Department of Histology and Embryology, Faculty of Medicine, Ege University, Izmir, Turkiye.
  • Çavusoglu T; Department of Histology and Embryology, Faculty of Medicine, Izmir Bakirçay University, Izmir, Turkiye.
  • Kiliç KD; Department of Histology and Embryology, Faculty of Medicine, Ege University, Izmir, Turkiye.
  • Sirin C; Department of Histology and Embryology, Faculty of Medicine, Ege University, Izmir, Turkiye.
  • Tomruk C; Department of Histology and Embryology, Republic of Turkiye Ministry of Health Samsun Education and Research Hospital, Samsun, Turkiye.
  • Yigittürk G; Department of Histology and Embryology, Faculty of Medicine, Mugla Sitki Koçman University, Mugla, Turkiye.
  • Erbas O; Department of Physiology, Faculty of Medicine, Demiroglu Bilim University, Istanbul, Turkiye.
  • Yildirim Sözmen E; Department of Medical Biochemistry, Faculty of Medicine, Ege University, Izmir, Turkiye.
  • Baka M; Department of Histology and Embryology, Faculty of Medicine, Ege University, Izmir, Turkiye.
Turk J Med Sci ; 53(5): 1281-1292, 2023.
Article en En | MEDLINE | ID: mdl-38813015
ABSTRACT
Background/

aim:

The subject of this study was to investigate the utility of platelet-rich plasma (PRP) in the cryopreservation process to reduce cryodamage and increase tissue viability. Materials and

methods:

Twenty-one female Wistar rats were randomly allocated to three groups. In Group 1 (G1), rats were not subjected to vitrification (n = 7). Group 2 (G2) was the vitrification group in which PRP was added to the basic vitrification solution (n = 7). Group 3 (G3) was the vitrification group in which fetal bovine serum was added to the basic vitrification solution (n = 7). Warmed tissues were evaluated with histochemical (HC) and immunohistochemical (IHC) staining, the TUNEL method, immunofluorescence (IF) staining, and biochemical analyses.

Results:

The percentages of IHC staining, TUNEL method positivity, and IF staining were significantly higher in G2 compared to both G1 and G3 (P < 0.05). G2 ovaries exhibited a significant increase in both malondialdehyde and catalase values in comparison to G1 (P < 0.05). In HC staining, degenerations in primary and secondary follicles and in ovarian tissue were more common in the PRP-supplemented group. The calcium used in PRP activation was suspected to have increased the degeneration and prevented the possible positive effects of PRP.

Conclusion:

To the best of our knowledge, PRP-supplemented vitrification solution was used for the first time in the literature in this study in whole rat ovarian tissue vitrification. If PRP is to be used as a component in vitrification solution for rat ovarian tissue, the use of lower amounts of calcium or different methods in PRP activation, or the use of nonactivated PRP, should be considered from the beginning.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ovario / Criopreservación / Ratas Wistar / Plasma Rico en Plaquetas / Vitrificación Límite: Animals Idioma: En Revista: Turk J Med Sci Año: 2023 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ovario / Criopreservación / Ratas Wistar / Plasma Rico en Plaquetas / Vitrificación Límite: Animals Idioma: En Revista: Turk J Med Sci Año: 2023 Tipo del documento: Article