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Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers.
Rondon, Rodolfo; Valdés, Catalina; Cosseau, Céline; Bergami, Elisa; Cárdenas, César Antonio; Balbi, Teresa; Pérez-Toledo, Carolina; Garrido, Ignacio; Perrois, Garance; Chaparro, Cristian; Corre, Erwan; Corsi, Ilaria; González-Aravena, Marcelo.
Afiliación
  • Rondon R; Departamento Científico, Instituto Antártico Chileno, Punta Arenas, Chile. Electronic address: rrondon@inach.cl.
  • Valdés C; Departamento Científico, Instituto Antártico Chileno, Punta Arenas, Chile; The University of Texas Health Science Center at Houston, Houston, USA.
  • Cosseau C; IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Perpignan, France.
  • Bergami E; Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
  • Cárdenas CA; Departamento Científico, Instituto Antártico Chileno, Punta Arenas, Chile; Millenium Institute Biodiversity of Antarctic and Subantarctic Ecosystems (BASE), Santiago, Chile.
  • Balbi T; Department of Earth Environment & Life Sciences, University of Genoa, Genoa, Italy.
  • Pérez-Toledo C; Departamento Científico, Instituto Antártico Chileno, Punta Arenas, Chile.
  • Garrido I; Centro de Investigaciones Dinámica de Ecosistemas Marinos de Altas Latitudes, Valdivia, Chile; Instituto de Ciencias Marinas y Limnológicas, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
  • Perrois G; Departamento Científico, Instituto Antártico Chileno, Punta Arenas, Chile; Tropical & Subtropical Research Center, Korea Institute of Ocean Science and Technology, Jeju 63349, the Republic of Korea.
  • Chaparro C; IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Perpignan, France.
  • Corre E; Faculté de Sciences, CNRS, FR 2424 CNRS, ABIMS, Station Biologique de Roscoff, Université Sorbonne, Roscoff, France.
  • Corsi I; Department of Physical, Earth and Environmental Sciences, University of Siena, Siena, Italy.
  • González-Aravena M; Departamento Científico, Instituto Antártico Chileno, Punta Arenas, Chile.
Ecotoxicol Environ Saf ; 280: 116523, 2024 Jul 15.
Article en En | MEDLINE | ID: mdl-38850707
ABSTRACT
In recent years micro- and nanoplastics and metal-oxide nanomaterials have been found in several environmental compartments. The Antarctic soft clam Laternula elliptica is an endemic Antarctic species having a wide distribution in the Southern Ocean. Being a filter-feeder, it could act as suitable bioindicator of pollution from nanoparticles also considering its sensitivity to various sources of stress. The present study aims to assess the impact of polystyrene nanoparticles (PS-NP) and the nanometal titanium-dioxide (n-TiO2) on genome-wide transcript expression of L. elliptica either alone and in combination and at two toxicological relevant concentrations (5 and 50 µg/L) during 96 h exposure. Transcript-target qRT-PCR was performed with the aim to identify suitable biomarkers of exposure and effects. As expected, at the highest concentration tested, the clustering was clearer between control and exposed clams. A total of 221 genes resulted differentially expressed in exposed clams and control ones, and 21 of them had functional annotation such as ribosomal proteins, antioxidant, ion transport (osmoregulation), acid-base balance, immunity, lipid metabolism, cell adhesion, cytoskeleton, apoptosis, chromatin condensation and cell signaling. At functional level, relevant transcripts were shared among some treatments and could be considered as general stress due to nanoparticle exposure. After applying transcript-target approach duplicating the number of clam samples, four ecologically relevant transcripts were revealed as biomarkers for PS-NP, n-TiO2 and their combination at 50 µg/L, that could be used for monitoring clams' health status in different Antarctic localities.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Titanio / Contaminantes Químicos del Agua / Bivalvos / Nanopartículas / Transcriptoma Límite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Titanio / Contaminantes Químicos del Agua / Bivalvos / Nanopartículas / Transcriptoma Límite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Año: 2024 Tipo del documento: Article