Your browser doesn't support javascript.
loading
African swine fever virus pB475L evades host antiviral innate immunity via targeting STAT2 to inhibit IFN-I signaling.
Huang, Zhao; Mai, Zhanzhuo; Kong, Cuiying; You, Jianyi; Lin, Sizhan; Gao, Chenyang; Zhang, WenBo; Chen, Xiongnan; Xie, Qingmei; Wang, Heng; Tang, Shengqiu; Zhou, Pei; Gong, Lang; Zhang, Guihong.
Afiliación
  • Huang Z; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University,
  • Mai Z; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; African Swine Fever Regional Laboratory of China, South China Agricultural University, Guangzhou, China.
  • Kong C; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
  • You J; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; African Swine Fever Regional Laboratory of China, South China Agricultural University, Guangzhou, China.
  • Lin S; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; African Swine Fever Regional Laboratory of China, South China Agricultural University, Guangzhou, China.
  • Gao C; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; African Swine Fever Regional Laboratory of China, South China Agricultural University, Guangzhou, China.
  • Zhang W; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; African Swine Fever Regional Laboratory of China, South China Agricultural University, Guangzhou, China.
  • Chen X; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University,
  • Xie Q; College of Animal Science, South China Agricultural University, Guangzhou, China.
  • Wang H; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; African Swine Fever Regional Laboratory of China, South China Agricultural University, Guangzhou, China; Research Center for African Swine F
  • Tang S; Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan University, Shaoguan, China.
  • Zhou P; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; African Swine Fever Regional Laboratory of China, South China Agricultural University, Guangzhou, China. Electronic address: zhoupei@scau.ed
  • Gong L; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; African Swine Fever Regional Laboratory of China, South China Agricultural University, Guangzhou, China. Electronic address: gonglang@scau.e
  • Zhang G; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Research Center for African Swine Fever Prevention and Control, South China Agricultural University, Guangzhou, China; Maoming Branch, Guang
J Biol Chem ; 300(7): 107472, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38879005
ABSTRACT
African swine fever virus (ASFV) causes severe disease in domestic pigs and wild boars, seriously threatening the development of the global pig industry. Type I interferon (IFN-I) is an important component of innate immunity, inducing the transcription and expression of antiviral cytokines by activating Janus-activated kinase-signal transducer and activator of transcription (STAT). However, the underlying molecular mechanisms by which ASFV antagonizes IFN-I signaling have not been fully elucidated. Therefore, using coimmunoprecipitation, confocal microscopy, and dual luciferase reporter assay methods, we investigated these mechanisms and identified a novel ASFV immunosuppressive protein, pB475L, which interacts with the C-terminal domain of STAT2. Consequently, pB475L inhibited IFN-I signaling by inhibiting STAT1 and STAT2 heterodimerization and nuclear translocation. Furthermore, we constructed an ASFV-B475L7PM mutant strain by homologous recombination, finding that ASFV-B475L7PM attenuated the inhibitory effects on IFN-I signaling compared to ASFV-WT. In summary, this study reveals a new mechanism by which ASFV impairs host innate immunity.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Virales / Transducción de Señal / Interferón Tipo I / Virus de la Fiebre Porcina Africana / Factor de Transcripción STAT2 / Inmunidad Innata Límite: Animals / Humans Idioma: En Revista: J Biol Chem Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Virales / Transducción de Señal / Interferón Tipo I / Virus de la Fiebre Porcina Africana / Factor de Transcripción STAT2 / Inmunidad Innata Límite: Animals / Humans Idioma: En Revista: J Biol Chem Año: 2024 Tipo del documento: Article