Your browser doesn't support javascript.
loading
Methanolic Extract and Brominated Compound from the Brazilian Marine Sponge Aplysina fulva Are Neuroprotective and Modulate Inflammatory Profile of Microglia.
Nunes, Catarina de Jesus; Santos, Cinthia Cristina; Soares, Erica Novaes; Lima, Irlã Santos; Alves, Uesley Vieira; Lanna, Emílio; Batista, Ronan; do Nascimento, Ravena Pereira; Costa, Silvia Lima.
Afiliación
  • Nunes CJ; Laboratory of Neurochemistry and Cell Biology (LabNq), Department of Biochemistry and Biophysics, Institute of Science and Health, Federal University of Bahia, Salvador 40231-300, Bahia, Brazil.
  • Santos CC; Laboratory of Neurochemistry and Cell Biology (LabNq), Department of Biochemistry and Biophysics, Institute of Science and Health, Federal University of Bahia, Salvador 40231-300, Bahia, Brazil.
  • Soares EN; Laboratory of Neurochemistry and Cell Biology (LabNq), Department of Biochemistry and Biophysics, Institute of Science and Health, Federal University of Bahia, Salvador 40231-300, Bahia, Brazil.
  • Lima IS; Laboratory of Neurochemistry and Cell Biology (LabNq), Department of Biochemistry and Biophysics, Institute of Science and Health, Federal University of Bahia, Salvador 40231-300, Bahia, Brazil.
  • Alves UV; Laboratory of Research in Bioactive Substances (LAPESBI), Department of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador 40170-115, Bahia, Brazil.
  • Lanna E; Biology Institute, Federal University of Bahia, Salvador 40170-115, Bahia, Brazil.
  • Batista R; Laboratory of Research in Bioactive Substances (LAPESBI), Department of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador 40170-115, Bahia, Brazil.
  • do Nascimento RP; Laboratory of Neurochemistry and Cell Biology (LabNq), Department of Biochemistry and Biophysics, Institute of Science and Health, Federal University of Bahia, Salvador 40231-300, Bahia, Brazil.
  • Costa SL; Laboratory of Neurochemistry and Cell Biology (LabNq), Department of Biochemistry and Biophysics, Institute of Science and Health, Federal University of Bahia, Salvador 40231-300, Bahia, Brazil.
Mar Drugs ; 22(6)2024 May 22.
Article en En | MEDLINE | ID: mdl-38921546
ABSTRACT
Neurodegenerative diseases involve neuroinflammation and a loss of neurons, leading to disability and death. Hence, the research into new therapies has been focused on the modulation of the inflammatory response mainly by microglia/macrophages. The extracts and metabolites of marine sponges have been presented as anti-inflammatory. This study evaluated the toxicity of an extract and purified compound from the Brazilian marine sponge Aplysina fulva as well as its neuroprotection against inflammatory damage associated with the modulation of microglia response. PC12 neuronal cells and neonatal rat microglia were treated with the methanolic extract of A. fulva (AF-MeOH, 0.1-200 µg/mL) or with its purified dimethyl ketal of 3,5-dibromoverongiaquinol (AF-H1, 0.1-100 µM). Cytotoxicity was determined by MTT tetrazolium, Trypan blue, and propidium iodide; microglia were also treated with the conditioned medium (CM) from PC12 cells in different conditions. The microglia phenotype was determined by the expression of Iba-1 and CD68. AF-MeOH and AF-H1 were not toxic to PC12 or the microglia. Inflammatory damage with Escherichia coli lipopolysaccharide (LPS, 5 µg/mL) was not observed in the PC12 cells treated with AF-MeOH (1-10 µg/mL) or AF-H1 (1-10 µM). Microglia subjected to the CM from PC12 cells treated with LPS and AF-MeOH or AF-H1 showed the control phenotype-like (multipolar, low-CD68), highlighting the anti-neuroinflammatory and neuroprotective effect of components of this marine sponge.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Poríferos / Microglía / Fármacos Neuroprotectores Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Mar Drugs Asunto de la revista: BIOLOGIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Poríferos / Microglía / Fármacos Neuroprotectores Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Mar Drugs Asunto de la revista: BIOLOGIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil