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Viperidae snakes infected by mammalian-associated trypanosomatids and a free-living kinetoplastid.
Nantes, Wesley Arruda Gimenes; Liberal, Sany Caroline; Santos, Filipe Martins; Dario, Maria Augusta; Mukoyama, Lincoln Takashi Hota; Woidella, Katrine Berres; Rita, Paula Helena Santa; Roque, André Luiz Rodrigues; de Oliveira, Carina Elisei; Herrera, Heitor Miraglia; Jansen, Ana Maria.
Afiliación
  • Nantes WAG; Environmental Sciences and Agricultural Sustainability Postgraduation, Dom Bosco Catholic University, Campo Grande, 79117-900, Brazil. Electronic address: wesley_anantes@hotmail.com.
  • Liberal SC; Environmental Sciences and Agricultural Sustainability Postgraduation, Dom Bosco Catholic University, Campo Grande, 79117-900, Brazil.
  • Santos FM; Environmental Sciences and Agricultural Sustainability Postgraduation, Dom Bosco Catholic University, Campo Grande, 79117-900, Brazil.
  • Dario MA; Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil.
  • Mukoyama LTH; Biotechnology Postgraduation, Dom Bosco Catholic University, Campo Grande 79117-900, Brazil.
  • Woidella KB; Biomedicine undergraduate, Dom Bosco Catholic University, Campo Grande 79117-900, Brazil.
  • Rita PHS; Bioterium, Dom Bosco Catholic University, Campo Grande 79117-900, Brazil.
  • Roque ALR; Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil.
  • de Oliveira CE; Environmental Sciences and Agricultural Sustainability Postgraduation, Dom Bosco Catholic University, Campo Grande, 79117-900, Brazil; Biotechnology Postgraduation, Dom Bosco Catholic University, Campo Grande 79117-900, Brazil.
  • Herrera HM; Environmental Sciences and Agricultural Sustainability Postgraduation, Dom Bosco Catholic University, Campo Grande, 79117-900, Brazil; Biotechnology Postgraduation, Dom Bosco Catholic University, Campo Grande 79117-900, Brazil.
  • Jansen AM; Environmental Sciences and Agricultural Sustainability Postgraduation, Dom Bosco Catholic University, Campo Grande, 79117-900, Brazil; Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil.
Infect Genet Evol ; 123: 105630, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38936526
ABSTRACT
Trypanosomatids have achieved significant evolutionary success in parasitizing various groups, yet reptiles remain relatively unexplored. The utilization of advanced molecular tools has revealed an increased richness of trypanosomatids in vertebrate hosts. The aim of this study was to identify the trypanosomatid species infecting Bothrops moojeni and Crotalus durissus kept in captivity from 2000 to 2022. Blood samples were obtained from 106 snakes 73C. durissus and 33 B. moojeni. Whole blood was collected for hemoculture, blood smears and centrifugated to obtain the blood clot that had its DNA extracted and submitted to Nested PCR (18S rDNA gene) to detect Trypanosomatidae. Positive samples were quantified and submitted to both conventional (Sanger) and next generation sequencing (NGS). Cloning of the amplified PCR product was performed for only one individual of C. durissus. To exclude the possibility of local vector transmission, attempts to capture sandflies were conducted using six CDC-LT type light traps. Molecular diagnosis revealed that 34% of the snakes presented trypanosomatid DNA, 47.94% in C. durissus and 3.9% in B. moojeni. The cloning process generated four colonies identified as a new MOTU named Trypanosomatidae sp. CROT. The presence of DNA of five trypanosomatids (Trypanosoma cruzi TcII/VI, Trypanosoma sp. DID, Trypanosoma cascavelli, Trypanosomatidae sp. CROT, Leishmania infantum and Leishmania sp.) and one free-living kinetoplastid (Neobodo sp.) was revealed through NGS and confirmed by phylogenetic analysis. The haplotypic network divided the T. cascavelli sequences into two groups, 1) marsupials and snakes and 2) exclusive to marsupials. Therefore, the diversity of Kinetoplastea is still underestimated. Snakes have the ability to maintain infection with T. cruzi and L. infantum for up to 20 years and the DNA finding of Neobodo sp. in the blood of a C. durissus suggests that this genus can infect vertebrates.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Filogenia Límite: Animals Idioma: En Revista: Infect Genet Evol Asunto de la revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Filogenia Límite: Animals Idioma: En Revista: Infect Genet Evol Asunto de la revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Año: 2024 Tipo del documento: Article