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Exploring the potential of genetic analysis in historical blood spots for patients with iodine-deficient goiter and thyroid carcinomas in Switzerland and Germany (1929-1989).
Schulte, Janine; Hotz, Gerhard; Szinnai, Gabor; Christ, Emanuel; Foderà, Gaspare; Krüsi, Karl; Nussberger, Peter; Kron, Sarah; Schulz, Iris.
Afiliación
  • Schulte J; Health Department Basel-Stadt, Institute of Forensic Medicine, University Basel, Pestalozzistrasse 22, Basel, 4056, Switzerland.
  • Hotz G; Natural History Museum Basel, Augustinergasse 2, Basel, 4001, Switzerland.
  • Szinnai G; Pediatric Endocrinology/Diabetology, University Children's Hospital Basel UKBB, University of Basel, Basel, 4056, Switzerland.
  • Christ E; Division of Endocrinology, Diabetology, Metabolism and Center of Endocrine and Neuroendocrine Tumors, University Hospital Basel, Basel, 4056, Switzerland.
  • Foderà G; Documentation Center, Municipal Administration, Riehen, 4125, Switzerland.
  • Krüsi K; Documentation Center, Municipal Administration, Riehen, 4125, Switzerland.
  • Nussberger P; Surgery Department, Adullam Hospital, Riehen, 4125, Switzerland.
  • Kron S; Health Department Basel-Stadt, Institute of Forensic Medicine, University Basel, Pestalozzistrasse 22, Basel, 4056, Switzerland.
  • Schulz I; Health Department Basel-Stadt, Institute of Forensic Medicine, University Basel, Pestalozzistrasse 22, Basel, 4056, Switzerland. iris.schulz@bs.ch.
BMC Med Genomics ; 17(1): 171, 2024 Jun 28.
Article en En | MEDLINE | ID: mdl-38943113
ABSTRACT
Iodine deficiency-induced goiter continues to be a global public health concern, with varying manifestations based on geography, patient's age, and sex. To gain insights into clinical occurrences, a retrospective study analyzed medical records from patients with iodine deficiency-induced goiter or thyroid cancer who underwent surgery at the Community Hospital in Riehen, Switzerland, between 1929 and 1989. Despite today's adequate iodine supplementation, a significant risk for iodine-independent goiter remains in Switzerland, suggesting that genetic factors, among others, might be involved. Thus, a pilot study exploring the feasibility of genetic analysis of blood spots from these medical records was conducted to investigate and enhance the understanding of goiter development, potentially identify genetic variations, and explore the influence of dietary habits and other environmental stimuli on the disease.Blood prints from goiter patients' enlarged organs were collected per decade from medical records. These prints had been made by pressing, drawing, or tracing (i.e., pressed and drawn) the removed organs onto paper sheets. DNA analysis revealed that its yields varied more between the prints than between years. A considerable proportion of the samples exhibited substantial DNA degradation unrelated to sample collection time and DNA mixtures of different contributors. Thus, each goiter imprint must be individually evaluated and cannot be used to predict the success rate of genetic analysis in general. Collecting a large sample or the entire blood ablation for genetic analysis is recommended to mitigate potential insufficient DNA quantities. Researchers should also consider degradation and external biological compounds' impact on the genetic analysis of interest, with the dominant contributor anticipated to originate from the patient's blood.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias de la Tiroides / Bocio / Yodo Límite: Adult / Female / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: BMC Med Genomics Asunto de la revista: GENETICA MEDICA Año: 2024 Tipo del documento: Article País de afiliación: Suiza

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias de la Tiroides / Bocio / Yodo Límite: Adult / Female / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: BMC Med Genomics Asunto de la revista: GENETICA MEDICA Año: 2024 Tipo del documento: Article País de afiliación: Suiza