Protocol to process fresh human cerebral cortex biopsies for patch-clamp recording and immunostaining.
STAR Protoc
; 5(4): 103313, 2024 Sep 17.
Article
en En
| MEDLINE
| ID: mdl-39292560
ABSTRACT
Cerebral cortex biopsies enable the investigation of native developing and mature human brain tissue. Here, we present a protocol to process human cortical biopsies from the surgical theater to the laboratory. We describe steps for the preparation of viable acute slices for patch-clamp recording using dedicated chemical solutions for transport and sectioning. We then explain procedures for tissue fixation and post hoc immunostaining to correlate physiological properties to morphological features and protein detection. For complete details on the use and execution of this protocol, please refer to Libé-Philippot et al.1.
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1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Año:
2024
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Article