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Copper chelate affinity chromatography of human fibroblast and leucocyte interferons.
J Gen Virol ; 43(3): 701-6, 1979 Jun.
Article en En | MEDLINE | ID: mdl-479851
ABSTRACT
Human fibroblast and human leucocyte interferons display a strong affinity for the copper chelate of bis-carboxymethyl amino agarose, binding tenaciously over a wide pH range (7.4 to 4.0). Their binding is apparently irreversible on a sorbent saturated with copper (24.8 mumol of Cu2+/ml of column bed). However, both interferons can be partially recovered from sorbents of lower copper content, prepared by leaching the columns with sodium citrate at pH 9.0. The recovery of fibroblast interferon from a leached sorbent (5.8 mumol of Cu2+/ml of column bed) is about 30% and that of leucocyte interferon about 60%. Moreover, the strength of binding of leucocyte interferon can be modulated by leaching copper chelate-agarose with citrate of varying concentration.
Asunto(s)
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Cromatografía de Afinidad / Interferones / Fibroblastos / Leucocitos Límite: Humans Idioma: En Revista: J Gen Virol Año: 1979 Tipo del documento: Article
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Cromatografía de Afinidad / Interferones / Fibroblastos / Leucocitos Límite: Humans Idioma: En Revista: J Gen Virol Año: 1979 Tipo del documento: Article