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The role of cell swelling and haemolysis in Sendai virus-induced cell fusion and in the diffusion of incorporated viral antigens.
J Cell Sci ; 42: 153-67, 1980 Apr.
Article en En | MEDLINE | ID: mdl-6249830
The role of the haemolytic activity of Sendai virus in cell-cell fusion has been examined in monolayers of human erythrocytes and erythrocyte ghosts fused with either haemolytic or non-haemolytic virus. Morphological observations indicate that cell swelling and haemolysis is a distinct event in cell-cell fusion irrespective of whether it is virally induced or, in the case of non-haemolytic virus, experimentally induced. Osmotic swelling appears to be the driving force by which cells which have established sites of membrane fusion expand such sites to form poly-erythrocytes. Immunofluorescent labelling of viral antigens incorporated into the erythrocyte membrane as a result of viral envelope-cell fusion indicates that diffusion of antigens in the plane of the membrane is restricted in intact erythrocytes and resealed erythrocyte ghosts but not in haemolysed erythrocytes or unsealed ghosts. A perturbation of the erythrocyte membrane resulting from osmotic lysis appears to form a prerequisite for the lateral diffusion of viral elements.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Virus de la Parainfluenza 1 Humana / Eritrocitos / Hemólisis Límite: Humans Idioma: En Revista: J Cell Sci Año: 1980 Tipo del documento: Article
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Virus de la Parainfluenza 1 Humana / Eritrocitos / Hemólisis Límite: Humans Idioma: En Revista: J Cell Sci Año: 1980 Tipo del documento: Article