[Immunological assay of apolipoprotein A-I]. / Dosage immunologique de l'apolipoprotéine A-I.
Ann Biol Clin (Paris)
; 40(3): 187-94, 1982.
Article
en Fr
| MEDLINE
| ID: mdl-6814305
ABSTRACT
Since lipid free apo A-I and lipid rich apo A-I (HDL, LP A, LP A-I) have different accessible antigenic sites, the electroimmunoassay ("rocket") does not allow the determination of the apo A-I concentration in both lipoproteins and serum when lipid free apo A-I are taken as controls. The results are found more significant when an isolated HDL or serum are used as reference. A comparison is made with the results (height of the pics) obtained for the same sample with different antibodies anti apo A-I, checked with the same HDL control, great differences two to one is observed. It is only explained by the various composition of antibodies directed against different apo A-I antigenic sites. Differences obtained in the levels of apo A-I in HDL2 and in HDL3 by immunoprecipitation show that the more lipoproteins are lipid rich the less their apo A-I are reached by antibodies.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Apolipoproteínas
Límite:
Animals
/
Humans
Idioma:
Fr
Revista:
Ann Biol Clin (Paris)
Año:
1982
Tipo del documento:
Article