The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen.
Am J Surg
; 170(5): 502-5, 1995 Nov.
Article
en En
| MEDLINE
| ID: mdl-7485743
ABSTRACT
BACKGROUND:
Thrombospondin (TSP), a cell matrix protein, and transforming growth factor beta (TGF-beta), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-beta on tumor cell invasion. MATERIALS ANDMETHODS:
Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (x 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-beta and the cysteine-serine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated.RESULTS:
TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-beta inhibited TSP-promoted invasion by 50% to 71%.CONCLUSIONS:
TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-beta.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Boca
/
Glicoproteínas de Membrana
/
Carcinoma de Células Escamosas
/
Moléculas de Adhesión Celular
/
Colágeno
Límite:
Humans
Idioma:
En
Revista:
Am J Surg
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos