Expression patterns of predicted genes from the C. elegans genome sequence visualized by FISH in whole organisms.
Nat Genet
; 11(3): 314-20, 1995 Nov.
Article
en En
| MEDLINE
| ID: mdl-7581456
ABSTRACT
More than 10 megabases of contiguous genome sequence have been submitted to the databases by the Caenorhabditis elegans Genome Sequencing Consortium. To characterize the genes predicted from the sequence, we have developed high resolution FISH for visualization of mRNA distributions in whole animals. The high resolution and sensitivity afforded by the use of directly fluorescently labelled probes and confocal imaging permitted mRNA distributions to be recorded at the cellular and subcellular level. Expression patterns were obtained for 8 out of 10 genes in an initial test set of predicted gene sequences, indicating that FISH is an effective means of characterizing predicted genes in C. elegans.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Caenorhabditis
/
Expresión Génica
/
Genoma
/
Hibridación Fluorescente in Situ
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Nat Genet
Asunto de la revista:
GENETICA MEDICA
Año:
1995
Tipo del documento:
Article
País de afiliación:
Reino Unido