Inhibition of the ATPase activity of P-glycoprotein by porphyrin photosensitization of multidrug-resistant cells in vitro.
Photochem Photobiol
; 61(4): 390-6, 1995 Apr.
Article
en En
| MEDLINE
| ID: mdl-7740083
ABSTRACT
The effectiveness of photodynamic therapy against P-glycoprotein ATPase activity in multidrug-resistant cells was studied. Chinese hamster ovary AUXB1 (drug-sensitive) and CR1R12 (multidrug-resistant) cell lines were compared with respect to uptake of 14C-polyhematoporphyrin and porphyrin photosensitization. Phototoxicity of Photofrin was similar in both cell lines, and no major differences in uptake or efflux of 14C-polyhematoporphyrin were observed. Porphyrin photosensitization in vitro of CR1R12 cells or isolated plasma membranes from these cells caused inhibition of P-glycoprotein ATPase activity. Application of porphyrin photosensitization at a sublethal level to CR1R12 cells resulted in a small but significant increase in adriamycin-induced cytotoxicity. The hydrophobic "picket-fence" porphyrin, meso-tetrakis-(o-propionamidophenyl)porphyrin, alpha,alpha,alpha,beta-isomer, was more inhibitory toward P-glycoprotein ATPase activity than the two less hydrophobic porphyrins tetraphenylporphine tetrasulfonate and Photofrin.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Porfirinas
/
Adenosina Trifosfatasas
/
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Photochem Photobiol
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos