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Presence of N-methyl-D-aspartate (NMDA) receptors in neuroblastoma x glioma hybrid NG108-15 cells-analysis using [45Ca2+]influx and [3H]MK-801 binding as functional measures.
Ohkuma, S; Katsura, M; Chen, D Z; Chen, S H; Kuriyama, K.
Afiliación
  • Ohkuma S; Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.
Brain Res Mol Brain Res ; 22(1-4): 166-72, 1994 Mar.
Article en En | MEDLINE | ID: mdl-7912400
In the present study, we have attempted to clarify whether neuroblastoma glioma hybrid NG 108-15 cells (NG cells) possess the NMDA receptor complex using [45Ca2+]influx and [3H]MK-801 binding as functional measures. Glutamate and NMDA dose-dependently increased [45Ca2+]influx and these increases were further enhanced by glycine. Scatchard analysis revealed the presence of a high-affinity binding site for [3H]MK-801 with a KD of 18.8 nM and a Bmax of 0.328 pmol/mg protein. This [3H]MK-801 binding was also increased by NMDA in a dose-dependent manner and this increase was further enhanced by glycine. Both ketamine and MK-801 inhibited glutamate- and NMDA-induced [45Ca2+]influx as well as the increase of [3H]MK-801 binding in a dose-dependent manner. Similarly, Mg2+ and Zn2+ dose-dependently reduced both glutamate-induced [45Ca2+]influx and [3H]MK-801 binding. Spermine, one of the polyamines, showed a biphasic stimulatory effects on glutamate-induced [45Ca2+]influx and [3H]MK-801 binding. These results indicate that NG cells possess a pharmacologically distinct NMDA receptor complex and suggest that these cells may be useful for the analyses on pharmacological and biochemical characteristics of the NMDA receptor complex.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Maleato de Dizocilpina / Receptores de N-Metil-D-Aspartato / Células Híbridas Idioma: En Revista: Brain Res Mol Brain Res Asunto de la revista: BIOLOGIA MOLECULAR / CEREBRO Año: 1994 Tipo del documento: Article País de afiliación: Japón
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Maleato de Dizocilpina / Receptores de N-Metil-D-Aspartato / Células Híbridas Idioma: En Revista: Brain Res Mol Brain Res Asunto de la revista: BIOLOGIA MOLECULAR / CEREBRO Año: 1994 Tipo del documento: Article País de afiliación: Japón