Dietary vitamin E reverses the effects of ultraviolet light irradiation on rat skin glycosaminoglycans.
Biochim Biophys Acta
; 1156(3): 239-44, 1993 Mar 21.
Article
en En
| MEDLINE
| ID: mdl-8461312
ABSTRACT
Chondroitin 4-sulfate (Ch 4-S), three dermatan sulfates (DS18, DS45a, DS45b) and hyaluronic acid (HA) were the major glycosaminoglycans (GAG) isolated from the skin of 4 groups of albino rats. The yields from Group 1 (control) were Ch 4-S, 0.015%; HA, 0.028% and DS (total), 0.098% (w/w). Traces of heparin were detected only in rats irradiated with ultraviolet (UV) light (Group II), in the GAG pool isolated with 45% ethanol. Yields increased by at least 28% (w/w) in Group II, but decreased, except HA's, also by at least 28%, below the level of the control, in irradiated rats that also ingested vitamin E (Group III). The sulfate composition of these GAG determined by infrared spectroscopy was as follows approx. 17% (w/w) for DS18, 21-30% for DS45a, 21-35% for DS45b and 26-44% for Ch 4-S. A 60-70% (mol/mol) N-acetylation of hexosamine in DS45 was estimated by Fourier transform 1H-NMR spectroscopy; the IdUA composition of this DS was 30-46% (mol/mol), and the uronic acid/hexosamine ratio ranged from 2.501 to 1.61. The data show UV light irradiation of rat skin to result in an abnormally elevated production of the major GAG and oversulfation of Ch 4-S and DS. These effects are reversed, except for the sulfation of DS45b, when the irradiated animals also ingest vitamin E.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Protectores contra Radiación
/
Piel
/
Rayos Ultravioleta
/
Vitamina E
/
Glicosaminoglicanos
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1993
Tipo del documento:
Article