Quantitative analysis of cell proliferation and orientation on substrata with uniform parallel surface micro-grooves.
Biomaterials
; 17(11): 1093-9, 1996 Jun.
Article
en En
| MEDLINE
| ID: mdl-8718969
In order to quantify the effect of the substrata surface topography on cellular behaviour, planar and micro-textured silicon substrata were produced and made suitable for cell culture by radio frequency glow discharge treatment. These substrata possessed parallel surface grooves with a groove and ridge width of 2.0 (SilD02), 5.0 (SilD05) and 10 microns (SilD10). Groove depth was approximately 0.5 micron. Rat dermal fibroblasts (RDFs) were cultured on these substrata and a tissue culture polystyrene control surface for 1, 2, 3, 5 and 7 days. After incubation the cell proliferation was quantified with a Coulter Counter, and RDF size, shape and orientation with digital image analysis. Cell counts proved that neither the presence of the surface grooves nor the dimension of these grooves had an effect on the cell proliferation. However, RDFs on SilD02, and to a lesser extent on SilD05 substrata, were elongated and aligned parallel to the surface grooves. Orientation of the RDFs on SilD10 substrata proved to be almost comparable to the SilD00 substrata. Finally, it was observed that the cells on the micro-textured substrata were capable of spanning the surface grooves.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Silicio
/
Técnicas Citológicas
Límite:
Animals
Idioma:
En
Revista:
Biomaterials
Año:
1996
Tipo del documento:
Article
País de afiliación:
Países Bajos