Analysis of PTEN/MMAC1 alterations in aerodigestive tract tumors.
Cancer Res
; 58(3): 509-11, 1998 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-9458098
ABSTRACT
PTEN/MMAC1 is a candidate tumor suppressor gene recently identified at chromosomal band 10q23. It is mutated in sporadic brain, breast, and prostate cancer and in the germ line of patients with hereditary Cowden disease. We searched for genetic alterations of the PTEN/MMAC1 gene in 39 primary head and neck cancers (HNSCCs), 42 primary non-small cell lung cancers (NSCLCs), 80 pancreatic cancer xenografts, and 37 cell lines and xenografts from colon, lung, and gastric cancers. Microsatellite analysis revealed loss of heterozygosity at markers near the gene in 41% of primary HNSCCs, 50% of NSCLCs, and 39% of the pancreatic cancers. Three cases of HNSCCs displayed homozygous deletion involving the gene. We sequenced the entire coding region of the PTEN/MMAC1 gene in the remaining tumors displaying loss of heterozygosity and found one terminating mutation in a HNSCC sample. Thus, a second inactivation event was observed in 4 of 39 primary HNSCC cases. By use of a protein truncation assay, one terminating mutation was also identified in one of eight NSCLC cell lines. Our results suggest that PTEN/MMAC1 gene inactivation plays a role in the genesis of some tumor types.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN de Neoplasias
/
Carcinoma de Células Escamosas
/
Proteínas Tirosina Fosfatasas
/
Carcinoma de Pulmón de Células no Pequeñas
/
Monoéster Fosfórico Hidrolasas
/
Proteínas Supresoras de Tumor
/
Neoplasias del Sistema Digestivo
/
Neoplasias de Cabeza y Cuello
/
Neoplasias Pulmonares
/
Proteínas de Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos