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1.
J Environ Sci (China) ; 148: 364-374, 2025 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-39095171

RESUMO

Increasing nitrogen and phosphorus discharge and decreasing sediment input have made silicon (Si) a limiting element for diatoms in estuaries. Disturbances in nutrient structure and salinity fluctuation can greatly affect metal uptake by estuarine diatoms. However, the combined effects of Si and salinity on metal accumulation in these diatoms have not been evaluated. In this study, we aimed to investigate how salinity and Si availability combine to influence the adsorption of metals by a widely distributed diatom Phaeodactylum tricornutum. Our data indicate that replete Si and low salinity in seawater can enhance cadmium and copper adsorption onto the diatom surface. At the single-cell level, surface potential was a dominant factor determining metal adsorption, while surface roughness also contributed to the higher metal loading capacity at lower salinities. Using a combination of non-invasive micro-test technology, atomic force microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy, we demonstrate that the diversity and abundance of the functional groups embedded in diatom cell walls vary with salinity and Si supply. This results in a change in the cell surface potential and transient metal influx. Our study provides novel mechanisms to explain the highly variable metal adsorption capacity of a model estuarine diatom.


Assuntos
Diatomáceas , Salinidade , Silício , Poluentes Químicos da Água , Adsorção , Silício/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/análise , Estuários , Água do Mar/química , Metais/química
2.
J Environ Sci (China) ; 148: 541-552, 2025 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-39095187

RESUMO

The ocean serves as a repository for various types of artificial nanoparticles. Nanoplastics (NPs) and nano zinc oxide (nZnO), which are frequently employed in personal care products and food packaging materials, are likely simultaneously released and eventually into the ocean with surface runoff. Therefore, their mutual influence and shared destiny in marine environment cannot be ignored. This study examined how nanomaterials interacted and transported through sea sand in various salinity conditions. Results showed that NPs remained dispersed in brine, while nZnO formed homoaggregates. In seawater of 35 practical salinity units (PSU), nZnO formed heteroaggregates with NPs, inhibiting NPs mobility and decreasing the recovered mass percentage (Meff) from 24.52% to 12.65%. In 3.5 PSU brackish water, nZnO did not significantly aggregate with NPs, and thus barely affected their mobility. However, NPs greatly enhanced nZnO transport with Meff increasing from 14.20% to 25.08%, attributed to the carrier effect of higher mobility NPs. Cotransport from brackish water to seawater was simulated in salinity change experiments and revealed a critical salinity threshold of 10.4 PSU, below which the mobility of NPs was not affected by coexisting nZnO and above which nZnO strongly inhibited NP transport. This study highlights the importance of considering the mutual influence and shared destiny of artificial nanoparticles in the marine environment and how their interaction and cotransport are dependent on changes in seawater salinity.


Assuntos
Águas Salinas , Salinidade , Água do Mar , Poluentes Químicos da Água , Óxido de Zinco , Óxido de Zinco/química , Água do Mar/química , Águas Salinas/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/análise , Porosidade , Microplásticos , Modelos Químicos , Nanopartículas Metálicas/química
3.
J Environ Sci (China) ; 149: 254-267, 2025 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-39181640

RESUMO

As a new electrochemical technology, capacitive deionization (CDI) has been increasingly applied in environmental water treatment and seawater desalination. In this study, functional groups modified porous hollow carbon (HC) were synthesized as CDI electrode material for removing Na+ and Cl- in salty water. Results showed that the average diameter of HC was approximately 180 nm, and the infrared spectrum showed that its surface was successfully modified with sulfonic and amino groups, respectively. The sulfonic acid functionalized HC (HC-S) showed better electrochemical and desalting performance than the amino-functionalized HC (HCN), with a maximum Faradic capacity of 287.4 F/g and an adsorptive capacity of 112.97 mg/g for NaCl. Additionally, 92.63% capacity retention after 100 adsorption/desorption cycles demonstrates the excellent stability of HC-S. The main findings prove that HC-S is viable as an electrode material for desalination by high-performance CDI applications.


Assuntos
Carbono , Eletrodos , Purificação da Água , Purificação da Água/métodos , Carbono/química , Porosidade , Adsorção , Água do Mar/química , Salinidade , Cloreto de Sódio/química
4.
Sci Rep ; 14(1): 21260, 2024 09 11.
Artigo em Inglês | MEDLINE | ID: mdl-39261551

RESUMO

In the present study, we developed and validated an experimental life support system (ELSS) designed to investigate coral reef associated bacterial communities. The microcosms in the ELSS consisted of coral reef sediment, synthetic seawater, and specimens of five benthic reef species. These included two hard corals Montipora digitata and Montipora capricornis, a soft coral Sarcophyton glaucum, a zoanthid Zoanthus sp., and a sponge Chondrilla sp.. Physicochemical parameters and bacterial communities in the ELSS were similar to those observed at shallow coral reef sites. Sediment bacterial evenness and higher taxonomic composition were more similar to natural-type communities at days 29 and 34 than at day 8 after transfer to the microcosms, suggesting microbial stabilization after an initial recovery period. Biotopes were compositionally distinct but shared a number of ASVs. At day 34, sediment specific ASVs were found in hosts and visa versa. Transplantation significantly altered the bacterial community composition of M. digitata and Chondrilla sp., suggesting microbial adaptation to altered environmental conditions. Altogether, our results support the suitability of the ELSS developed in this study as a model system to investigate coral reef associated bacterial communities using multi-factorial experiments.


Assuntos
Antozoários , Bactérias , Recifes de Corais , Microbiota , Animais , Antozoários/microbiologia , Bactérias/classificação , Bactérias/genética , Sistemas de Manutenção da Vida , Sedimentos Geológicos/microbiologia , Água do Mar/microbiologia
5.
Sci Rep ; 14(1): 21401, 2024 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-39271926

RESUMO

Current climate projections for mid-latitude regions globally indicate an intensification of wind-driven coastal upwelling due to warming conditions. The dynamics of mid-latitude coastal upwelling are marked by environmental variability across temporal scales, which affect key physiological processes in marine calcifying organisms and can impact their large-scale distribution patterns. In this context, marine invertebrates often exhibit phenotypic plasticity, enabling them to adapt to environmental change. In this study, we examined the physiological performance (i.e., metabolism, Thermal Performance Curves, and biomass and calcification rates) of individuals of the intertidal mollusk Chiton granosus, a chiton found from northern Peru to Cape Horn (5° to 55°S). Our spatial study design indicated a pattern of contrasting conditions among locations. The Talcaruca site, characterized by persistent upwelling and serving as a biogeographic break, exhibited lower pH and carbonate saturation states, along with higher pCO2, compared to the sites located to the north and south of this location (Huasco and Los Molles, respectively). In agreement with the spatial pattern in carbonate system parameters, long-term temperature records showed lower temperatures that changed faster over synoptic scales (1-15 days) at Talcaruca, in contrast to the more stable conditions at the sites outside the break. Physiological performance traits from individuals from the Talcaruca population exhibited higher values and more significant variability, along with significantly broader and greater warming tolerance than chitons from the Huasco and Los Molles populations. Moreover, marked changes in local abundance patterns over three years suggested population-level responses to the challenging environmental conditions at the biogeographic break. Thus, C. granosus from the Talcaruca upwelling zone represents a local population with wide tolerance ranges that may be capable of withstanding future upwelling intensification on the Southern Eastern Pacific coast and likely serving as a source of propagules for less adapted populations.


Assuntos
Temperatura , Animais , Ecossistema , Biomassa , Peru , Água do Mar , Adaptação Fisiológica , Mudança Climática , Calcificação Fisiológica
6.
Nutrients ; 16(17)2024 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-39275197

RESUMO

This study looked at how desalinated seawater, which has low minerals and high boron, could affect bone health. Prior research suggests that low mineral water may harm bone health and boron could be beneficial, but the overall impact on bone health is still unclear. Eighty-nine-week-old male Balb/C mice were allocated into eight groups and administered either tap water or purified water with varying boron concentrations (0, 5, 40, and 200 mg/L). They were kept in an environment mimicking tropical conditions (35-40 °C, 70-80% humidity) and underwent daily treadmill exercise for 13 weeks. At the 14th week, serum, femora, and lumbar vertebrae were collected for mineral metabolism, bone biomarker, microstructure, and biomechanics evaluation. Boron exposure improved bone formation, microstructure, and biomechanics initially but the benefits weakened with higher levels of exposure (p < 0.05). Co-exposure to purified water elevated serum boron but weakened the promotion of boron on bone minerals and the bone benefits of boron compared to tap water (p < 0.05). Thus, when studying the health effects of boron in desalinated seawater, it is crucial to look at various health effects beyond bone health. Furthermore, it is important to consider the mineral composition of drinking water when using boron for bone health benefits.


Assuntos
Osso e Ossos , Boro , Camundongos Endogâmicos BALB C , Águas Minerais , Água do Mar , Animais , Boro/farmacologia , Masculino , Água do Mar/química , Camundongos , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/metabolismo , Densidade Óssea/efeitos dos fármacos , Água Potável , Biomarcadores/sangue , Vértebras Lombares/efeitos dos fármacos , Fêmur/efeitos dos fármacos
7.
Int J Mol Sci ; 25(17)2024 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-39273589

RESUMO

In samples of harmful algal blooms (HABs), seawater can contain a high abundance of microorganisms and elemental ions. Along with the hardness of the walls of key HAB dinoflagellates such as Prorocentrum triestinum, this makes RNA extraction very difficult. These components interfere with RNA isolation, causing its degradation, in addition to the complex seawater properties of HABs that could hinder RNA isolation for effective RNA sequencing and transcriptome profiling. In this study, an RNA isolation technique was established through the modification of the Trizol method by applying the Micropestle System on cell pellets of P. triestinum frozen at -20 °C, obtained from 400 mL of culture with a total of 107 cells/mL. The results of the modified Trizol protocol generated quality RNA samples for transcriptomics sequencing, as determined by their measurement in Analyzer Agilent 4150.


Assuntos
Dinoflagellida , Dinoflagellida/genética , RNA/isolamento & purificação , RNA/genética , Guanidinas/química , Análise de Sequência de RNA/métodos , Proliferação Nociva de Algas , Perfilação da Expressão Gênica/métodos , Transcriptoma , Nucleotídeos/genética , Nucleotídeos/isolamento & purificação , Água do Mar , Fenóis
8.
Science ; 385(6714): eaab2661, 2024 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-39265021

RESUMO

Lipids comprise a significant fraction of sinking organic matter in the ocean and play a crucial role in the carbon cycle. Despite this, our understanding of the processes that control lipid degradation is limited. We combined nanolipidomics and imaging to study the bacterial degradation of diverse algal lipid droplets and found that bacteria isolated from marine particles exhibited distinct dietary preferences, ranging from selective to promiscuous degraders. Dietary preference was associated with a distinct set of lipid degradation genes rather than with taxonomic origin. Using synthetic communities composed of isolates with distinct dietary preferences, we showed that lipid degradation is modulated by microbial interactions. A particle export model incorporating these dynamics indicates that metabolic specialization and community dynamics may influence lipid transport efficiency in the ocean's mesopelagic zone.


Assuntos
Bactérias , Metabolismo dos Lipídeos , Oceanos e Mares , Fitoplâncton , Bactérias/metabolismo , Bactérias/genética , Bactérias/classificação , Gotículas Lipídicas/metabolismo , Interações Microbianas , Microbiota , Água do Mar/microbiologia , Água do Mar/química , Fitoplâncton/metabolismo
9.
Science ; 385(6714): 1167-1168, 2024 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-39265029
10.
Sci Data ; 11(1): 994, 2024 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-39266528

RESUMO

The Changjiang Estuary and adjacent East China Sea are well-known hypoxic aquatic environments. Eutrophication-driven hypoxia frequently occurs in coastal areas, posing a major threat to the ecological environment, including altering community structure and metabolic processes of marine organisms, and enhancing diversion of energy shunt into microbial communities. However, the responses of microbial communities and their metabolic pathways to coastal hypoxia remain poorly understood. Here, we studied the microbial communities collected from spatiotemporal samplings using metagenomic sequencing in the Changjiang Estuary and adjacent East China Sea. This generated 1.31 Tbp of metagenomics data, distributed across 103 samples corresponding to 8 vertical profiles. We further reported 1,559 metagenome-assembled genomes (MAGs), of which 508 were high-quality MAGs (Completeness > 90% and Contamination < 10%). Phylogenomic analysis classified them into 181 archaeal and 1,378 bacterial MAGs. These results provided a valuable metagenomic dataset available for further investigation of the effects of hypoxia on marine microorganisms.


Assuntos
Archaea , Metagenoma , China , Archaea/genética , Bactérias/genética , Bactérias/classificação , Oxigênio , Água do Mar/microbiologia , Filogenia , Metagenômica , Oceanos e Mares , Microbiota
11.
BMC Plant Biol ; 24(1): 854, 2024 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-39266948

RESUMO

Casuarina equisetifolia trees are used as windbreaks in subtropical and tropical coastal zones, while C. equisetifolia windbreak forests can be degraded by seawater atomization (SA) and seawater encroachment (SE). To investigate the mechanisms underlying the response of C. equisetifolia to SA and SE stress, the transcriptome and metabolome of C. equisetifolia seedlings treated with control, SA, and SE treatments were analyzed. We identified 737, 3232, 3138, and 3899 differentially expressed genes (SA and SE for 2 and 24 h), and 46, 66, 62, and 65 differentially accumulated metabolites (SA and SE for 12 and 24 h). The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that SA and SE stress significantly altered the expression of genes related to plant hormone signal transduction, plant-pathogen interaction, and starch and sucrose metabolism pathways. The accumulation of metabolites associated with the biosynthetic pathways of phenylpropanoid and amino acids, as well as starch and sucrose metabolism, and glycolysis/gluconeogenesis were significantly altered in C. equisetifolia subjected to SA and SE stress. In conclusion, C. equisetifolia responds to SA and SE stress by regulating plant hormone signal transduction, plant-pathogen interaction, biosynthesis of phenylpropanoid and amino acids, starch and sucrose metabolism, and glycolysis/gluconeogenesis pathways. Compared with SA stress, C. equisetifolia had a stronger perception and response to SE stress, which required more genes and metabolites to be regulated. This study enhances our understandings of how C. equisetifolia responds to two types of seawater stresses at transcriptional and metabolic levels. It also offers a theoretical framework for effective coastal vegetation management in tropical and subtropical regions.


Assuntos
Água do Mar , Estresse Fisiológico , Estresse Fisiológico/genética , Água do Mar/química , Transcriptoma , Regulação da Expressão Gênica de Plantas , Metaboloma , Plântula/genética , Plântula/fisiologia , Metabolômica , Multiômica
12.
Molecules ; 29(17)2024 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-39275045

RESUMO

Posidonia oceanica significantly contributes to the health of oceans and coastal areas; however, its progressive decline is becoming an increasing source of concern. The present preliminary study aims to assess the chemical parameters that describe the state of preservation of the aforementioned plant meadows located in the Tremiti Islands archipelago. To better understand the plants' response to external factors, the emission of biogenic volatile organic compounds (BVOCs) was investigated using Posidonia oceanica as a biological indicator. Subsequently, the heavy metal concentrations (Ag, Al, As, Ba, Be, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Sb, Se, Sn, Ti, Tl, V, Zn) in sediments, leaves, and seawater were determined and pollution indicators were calculated to assess the deviation from the natural background levels of sediments. The dimethyl sulfoniopropionate (DMSP) to dimethyl sulfoxide (DMSO) ratio was calculated to evaluate the oxidative stress levels in the meadows because the DMSP naturally present in Posidonia oceanica is oxidized to DMSO and decreases the ratio of DMSP/DMSO. BVOC analysis revealed dimethyl sulphide (DMS) as the most abundant molecule. Morphological features led to variations in metal concentrations across sampling sites, with sheltered bays displaying a higher metal content. Degradation is indicated by a greater DMSO content in the outer leaves. In accordance with the metal content, the bioindicator ratio confirms greater degradation on the south side, which aligns with increased oxidative stress.


Assuntos
Alismatales , Ilhas , Metais Pesados , Alismatales/química , Itália , Metais Pesados/análise , Compostos Orgânicos Voláteis/análise , Água do Mar/química , Água do Mar/análise , Monitoramento Ambiental/métodos , Sedimentos Geológicos/química , Sedimentos Geológicos/análise , Poluentes Químicos da Água/análise , Folhas de Planta/química , Estresse Oxidativo
13.
Microb Biotechnol ; 17(9): e70009, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39264362

RESUMO

Carotenoids are natural pigments utilized as colourants and antioxidants across food, pharmaceutical and cosmetic industries. They exist in carbon chain lengths of C30, C40, C45 and C50, with C40 variants being the most common. Bacterioruberin (BR) and its derivatives are part of the less common C50 carotenoid group, synthesized primarily by halophilic archaea. This study analysed the compositional characteristics of BR extract (BRE) isolated from 'Haloferax marinum' MBLA0078, a halophilic archaeon isolated from seawater near Yeoungheungdo Island in the Republic of Korea, and investigated its antioxidant activity and protective effect on lipopolysaccharide (LPS)-induced C2C12 myotube atrophy. The main components of BRE included all-trans-BR, monoanhydrobacterioruberin, 2-isopentenyl-3,4-dehydrorhodopin and all-trans-bisanhydrobacterioruberin. BRE exhibited higher antioxidant activity and DNA nicking protection activity than other well-known C40 carotenoids, such as ß-carotene, lycopene and astaxanthin. In C2C12 myotubes, LPS treatment led to a reduction in myotube diameter and number, as well as the hypertranscription of the muscle-specific ubiquitin ligase MAFbx and MuRF1. BRE mitigated these changes by activating the Akt/mTOR pathway. Furthermore, BRE abolished the elevated cellular reactive oxygen species levels and the inflammation response induced by LPS. This study demonstrated that 'Hfx. marinum' is an excellent source of natural microbial C50 carotenoids with strong antioxidant capacity and may offer potential protective effects against muscle atrophy.


Assuntos
Antioxidantes , Carotenoides , Lipopolissacarídeos , Fibras Musculares Esqueléticas , Antioxidantes/farmacologia , Animais , Camundongos , Fibras Musculares Esqueléticas/efeitos dos fármacos , Fibras Musculares Esqueléticas/metabolismo , Linhagem Celular , Carotenoides/farmacologia , República da Coreia , Água do Mar/microbiologia
14.
Artigo em Inglês | MEDLINE | ID: mdl-39264709

RESUMO

A Gram-stain-negative, yellow-pigmented, and strictly aerobic bacterium, designated as strain MSW5T, was isolated from seawater of the Yellow Sea in South Korea. The cells were non-motile rods exhibiting oxidase- and catalase-positive activities. Growth was observed at 15-25 °C (optimum, 25 °C) and pH 5.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 1.0-5.0% (w/v) NaCl (optimum, 2.0%). Menaquinone-6 was the sole respiratory quinone, and iso-C15 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C15 : 0 3-OH, and C15 : 1 ω6c were the major cellular fatty acids. Major polar lipids included phosphatidylethanolamine, two unidentified aminolipids, and three unidentified lipids. Phylogenetic analyses based on 16S rRNA gene sequences and 92 concatenated core protein sequences revealed that strain MSW5T formed a distinct lineage within the genus Polaribacter. The genome of strain MSW5T was 3582 kb in size with a 29.1 mol% G+C content. Strain MSW5T exhibited the highest similarity to Polaribacter atrinae WP25T, with a 97.9% 16S rRNA gene sequence similarity. However, the average nucleotide identity and digital DNA-DNA hybridization values were 79.4 and 23.3%, respectively, indicating that strain MSW5T represents a novel species. Based on its phenotypic, chemotaxonomic, and phylogenetic characteristics, strain MSW5T is proposed to represent a novel species, with the name Polaribacter ponticola sp. nov. The type strain is MSW5T (=KACC 22340T=NBRC 116025T). In addition, whole genome sequence comparisons and phenotypic features suggested that Polaribacter sejongensis and Polaribacter undariae belong to the same species, with P. undariae proposed as a later heterotypic synonym of P. sejongensis. An emended description of Polaribacter sejongensis is also proposed.


Assuntos
Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano , Ácidos Graxos , Filogenia , RNA Ribossômico 16S , Água do Mar , Análise de Sequência de DNA , Vitamina K 2 , RNA Ribossômico 16S/genética , Ácidos Graxos/análise , Água do Mar/microbiologia , República da Coreia , DNA Bacteriano/genética , Vitamina K 2/análogos & derivados , Vitamina K 2/análise , Fosfatidiletanolaminas , Hibridização de Ácido Nucleico , Bacteroidetes/genética , Bacteroidetes/classificação , Bacteroidetes/isolamento & purificação , Fosfolipídeos/análise , Fosfolipídeos/química
15.
Water Sci Technol ; 89(2): 319-332, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-39219132

RESUMO

Marine fungi communities play a crucial role in the recycling of nutrients, restoration of biological systems, and the overall functioning of ecosystems. While aquatic fungal communities do react to pollution, there is a significant lack of information regarding the changes in the fungal community's structure, caused by marine pollution. In this study, we aim to address this gap in knowledge by investigating the range and makeup of fungal species present in marine environments in a polluted bay in Tunisia, spanning a biodiversity hotspot (Monastir Bay). Sequence analysis of the internal transcribed spacer region from culturable mycobiome and physicochemical parameters were investigated at seven sites in the bay. A total of 32 fungal taxa were identified at the genus and/or species levels and were assigned to four major groups (Aspergillacae 37.5%, Dothiomyceta 21.87%, Sordariamyceta 28.12%, and Yeasts 12.5%) with a remarkable predominance of Aspergillus genus. Assessment of the Shannon-Wiener diversity index and the Simpson dominance index revealed that the highest species diversity index (0.84) was recorded at the Kheniss site. Our results suggest the existence of diverse fungal communities, can be considered a useful community model for further ecological and evolutionary study of fungi in the bay.


Assuntos
Baías , Biodiversidade , Fungos , Tunísia , Fungos/genética , Fungos/classificação , Fungos/isolamento & purificação , Baías/microbiologia , Micobioma , Mar Mediterrâneo , Água do Mar/microbiologia , Monitoramento Ambiental
16.
Water Sci Technol ; 89(2): 454-469, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-39219141

RESUMO

Nanofiltration (NF) has been used as the default sulfate removal process in platforms to treat seawater for water flooding. Seawater is generally pretreated by chlorination and cartridge filters to reduce fouling of the membranes; however, this pretreatment is insufficient to provide water quality high enough to maintain the productivity of the NF membranes. In this study, the performances of two different pretreatment routes were evaluated. Microfiltration (MF) was evaluated as a replacement for cartridge filters, and the advanced oxidation process UV/H2O2 was evaluated as an additional stage of pretreatment upstream of the cartridge filters. The permeability of the NF membranes after 12 h of seawater sulfate removal in a bench system was 4.4 L·h-1·m-2·bar-1 when the UV/H2O2 process was adopted as the pretreatment and 2.9 L·h-1·m-2·bar-1 when the MF process was adopted, compared to 1.6 L·h-1·m-2·bar-1 achieved for the pretreatment with the cartridge filter alone. These results indicate that NF membrane fouling was significantly higher when seawater was pretreated only by the cartridge filter in comparison to both proposed pretreatments. An economic analysis showed that both systems are economically viable and can potentially reduce the operational costs of the NF sulfate removal process on platforms.


Assuntos
Filtração , Água do Mar , Purificação da Água , Purificação da Água/métodos , Purificação da Água/instrumentação , Filtração/métodos , Filtração/instrumentação , Membranas Artificiais , Sulfatos/química , Nanotecnologia , Peróxido de Hidrogênio/química
17.
NPJ Biofilms Microbiomes ; 10(1): 78, 2024 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-39227595

RESUMO

Protists are less studied for their role and diversity in ecosystems. Notably, protists have played and still play an important role in microbialites. Microbialites, or lithified microbial mats, represent the oldest evidence of fossil biofilms (~3.5 Gyr). Modern microbialites may offer a unique proxy to study the potential role of protists within a geological context. We examined protist diversity in freshwater (Kelly and Pavilion Lake in British Columbia, Canada) and marine (Highborne Cay, Bahamas) to hypersaline (Shark Bay, Australia) microbialites to decipher their geomicrobiological role. The freshwater microbialite communities were clearly distinct from their marine and hypersaline counterparts. Chlorophytes had higher numerical abundance in freshwater microbialites; whereas pennate diatoms dominated numerically in marine microbialites. Despite the differences, protists across ecosystems may have adopted similar roles and functions. We suggest a consistent biogeochemical role of protists across microbialites globally; but that salinity may shape protist composition and evolution in these ecosystems.


Assuntos
Ecossistema , Salinidade , Colúmbia Britânica , Eucariotos/classificação , Biodiversidade , Água Doce/microbiologia , Biofilmes/crescimento & desenvolvimento , Água do Mar/microbiologia , Austrália , Diatomáceas/crescimento & desenvolvimento , Sedimentos Geológicos/microbiologia
18.
Artigo em Inglês | MEDLINE | ID: mdl-39235837

RESUMO

Two bacterial strains, Y60-23T and HN-65T, were isolated from marine sediment samples collected from Xiaoshi Island, Weihai, and Dongzhai Harbour, Haikou, PR China, respectively. Based on the 16S rRNA gene sequences, strain Y60-23T exhibited 96.0% similarity to its most related type strain Hyphobacterium vulgare KCTC 52487T, while strain HN-65T exhibited 97.3% similarity to its most related type strain Hyphobacterium indicum 2ED5T. The 16S rRNA gene sequence similarity between the two strains was 95.8%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains Y60-23T and HN-65T belonged to the genus Hyphobacterium. Cells of strains Y60-23T and HN-65T were rod-shaped, Gram-stain-negative, aerobic, non-motile, prosthecate and multiplied by binary fission. The major cellular fatty acids (>10.0%) of strain Y60-23T were C18 : 1 ω7c and C17 : 0, while those of strain HN-65T were iso-C17 : 1 ω9c, iso-C17 : 0 and C18 : 1 ω7c. The major respiratory quinone in both strains was ubiquinone-10 (Q-10) and the major polar lipids were monoglycosyl diglyceride, sulfoquinovosyl diacylglycerol and glucuronopyranosyl diglyceride. The genomic DNA G+C contents of strains Y60-23T and HN-65T were 63.9 and 60.7 mol%, respectively. The average nucleotide identity value between the two strains was 72.1% and the DNA-DNA hybridization value was 18.4%, clearly distinguishing them from each other. According to the results of the phenotypic, chemotaxonomic, phylogenetic and genomic analyses, the two strains represented two novel species within the genus Hyphobacterium, for which the names Hyphobacterium marinum sp. nov. and Hyphobacterium lacteum sp. nov. were proposed with the type strains Y60-23T (=MCCC 1H01433T=KCTC 8172T) and HN-65T (=MCCC 1H01434T=KCTC 8169T), respectively.


Assuntos
Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano , Ácidos Graxos , Sedimentos Geológicos , Filogenia , RNA Ribossômico 16S , Análise de Sequência de DNA , Sedimentos Geológicos/microbiologia , RNA Ribossômico 16S/genética , Ácidos Graxos/química , Ácidos Graxos/análise , DNA Bacteriano/genética , China , Hyphomicrobiaceae/genética , Hyphomicrobiaceae/classificação , Hyphomicrobiaceae/isolamento & purificação , Hibridização de Ácido Nucleico , Água do Mar/microbiologia , Ubiquinona/análogos & derivados , Fosfolipídeos/análise
20.
Harmful Algae ; 138: 102686, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39244229

RESUMO

Several Dinophysis species can produce potent lipophilic toxins that pose a risk to human health when contaminated seafood is consumed, especially filter-feeding bivalve mussels. In the mussel farms of the Northwestern Adriatic Sea, seawater and seafood are regularly monitored for the presence of Dinophysis species and their associated toxins, but the current methodological approaches, such as light microscopy determinations, require a long time to make results available to local authorities. A molecular qPCR-based assay can be used to quantify various toxic Dinophysis species in a shorter timeframe. However, this approach is not currently employed in official testing activities. In this study, field samples were collected monthly or bi-weekly over one year from various mussel farms along the Northwestern Adriatic coast. The abundance of Dinophysis species in the seawater was determined using both traditional microscopy and qPCR assays. In addition, the concentration of lipophilic toxins for DSP in mussel flesh was quantified using LC-MS/MS focusing on the okadaic acid group. Dinophysis spp. site-specific single cells were isolated and analysed by qPCR yielding a mean rDNA copy number per cell of 1.21 × 104 ± 1.81 × 103. The qPCR assay gave an efficiency of 98 % and detected up to 10 copies of the rDNA target gene. The qPCR and light microscopy determinations in environmental samples showed a significant positive correlation (Spearman rs = 0.57, p-value < 0.001) with a ratio of 2.24 between the two quantification methods, indicating that light microscopy estimates were generally 44.6 % lower than those obtained by the qPCR assay. The qPCR approach showed several advantages such as rapidity, sensitivity and efficiency over conventional microscopy analysis, showing its potential future role in phytoplankton monitoring under the Official Controls Regulations for shellfish.


Assuntos
Bivalves , Dinoflagellida , Animais , Dinoflagellida/genética , Dinoflagellida/classificação , Bivalves/química , Monitoramento Ambiental/métodos , Toxinas Marinhas/análise , Água do Mar/química , Aquicultura
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