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1.
Anat Histol Embryol ; 53(5): e13107, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39282745

RESUMO

The aim of this study was to identify the glandula uropygialis's macroanatomical and histological structure in male and female birds (stork, goose, eagle, pigeon, crow and sparrowhawk) that belong to different populations. A total of 58 specimens were used in the study. The study materials were delivered to our laboratory by nature conservation and national parks, and no animals were euthanised for this study. The longitudinal and transversal lengths, dorsoventral heights and feather length of the glandula uropygialis and papilla uropygialis were measured with a digital calliper. Haematoxylin-eosin and Masson trichrome staining were performed for histological examinations. The shape of the glandula uropygialis was found to be pear-shaped in pigeons and sparrowhawks, heart-shaped in geese, kidney-shaped in eagles and oval-fascule-shaped in storks. In the crow, the shape of the glandula uropygialis was different from the other species in that it was located dorso-caudally. Histomorphological examination of the glands of these species revealed that the gland generally consisted of two lobes, right and left, surrounded by connective tissue from the outside. The parenchyma of these lobes consisted of tubulo-alveolar secretory glands with a radial arrangement from the periphery to the centre. The glands within the lobes differed from the periphery to the centre and although the general histomorphological appearance was similar between bird species, some differences were observed between species. Similar stromal structure was observed in all bird species evaluated in the study. However, the reticular connective tissue forming the roof of the gland was found to be more developed in pigeons, especially in Masson's trichroma staining. The degenerative layer in the glands was more prominent in the eagle, followed by the goose and crow. Although a common general histological structure was observed among bird species, simple histomorphological differences were found between these formations, but no differences were found between the sexes. The results obtained will be compared with the findings of the aves class and will provide a data source for this special gland specific to birds. It is thought that the results obtained may help to determine the functional properties of the gland and contribute to the science of ornithology.


Assuntos
Aves , Animais , Feminino , Masculino , Aves/anatomia & histologia , Plumas/anatomia & histologia , Gansos/anatomia & histologia , Columbidae/anatomia & histologia
2.
Rural Remote Health ; 24(3): 9055, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39285734

RESUMO

INTRODUCTION: Geese harvesting is a longstanding cultural tradition deeply ingrained among the Omushkego Cree in Fort Albany First Nation, embodying a holistic approach to health that integrates Indigenous knowledge, community wellbeing, and resilience. Despite historical disruptions stemming from colonization and assimilation policies, women have played a pivotal role in preserving and passing down traditional practices. The significance of goose harvesting extends beyond providing a nutrient-rich and cost-effective food source; it serves as a vehicle for cultural preservation and education, particularly fostering language acquisition among children. Nevertheless, concerns persist regarding the potential decline in the transmission of Indigenous knowledge. The interruption of intergenerational knowledge transfer not only poses implications for overall wellbeing but also worsens historical trauma within the community. In response to these challenges, the Niska (goose) harvesting program was developed with an aim to revitalize community harvesting practices, with a specific focus on incorporating the perspectives of women, especially in the preparatory and smoking phases of the geese. METHODS: Omushkego Cree women were approached to participate. The study was conducted during the spring of 2018, and employed photovoice and semi-structured interviews that explored the impact of geese preparatory activities on the health and wellbeing of Indigenous women. RESULTS: Major themes from the qualitative data included the importance of knowledge sharing, cultural continuity, healing, and the profound connection to the land. Women emphasized the value of sharing acquired knowledge, passing on traditions, and maintaining a connection to their cultural identity. Cultural continuity, depicted through intergenerational teachings and experiences, emerged as crucial for overall wellbeing. Participants spoke of the healing dynamics derived from engaging in traditional activities, highlighting the positive impact on physical, mental, emotional, and spiritual wellbeing. The land was identified as a central element in this healing process, representing more than just a physical space but an extension of home, contributing to a sense of peace and tranquility. The land became a medium for transmitting cultural teachings, shaping identity, and sustaining a subsistence lifestyle. CONCLUSION: The study emphasizes the importance of future research including more female youth participants to uncover specific challenges and strengths within this demographic. Overall, the Niska program demonstrates a comprehensive approach that intertwines cultural revitalization, community engagement, and holistic wellbeing, emphasizing the need for interventions that go beyond immediate challenges to create enduring positive impacts on Indigenous communities.


Assuntos
Gansos , Saúde da Mulher , Humanos , Feminino , Ontário , Animais , Adulto , Promoção da Saúde/métodos , Promoção da Saúde/organização & administração , Canadenses Indígenas
3.
Emerg Microbes Infect ; 13(1): 2399970, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39221587

RESUMO

Multiple genotypes of highly pathogenic avian influenza (HPAI) H5 clade 2.3.4.4b viruses have caused epizootics in wild birds and poultry. The HPAI H5N1 genotype C virus caused a modest epizootic, whereas the occurrence of the HPAI H5N1 genotype AB virus in 2021 resulted in the largest avian influenza epizootic in Europe to date. Here we studied the pathogenicity of two HPAI H5N1 viruses by experimentally infecting chickens, Pekin ducks, Eurasian wigeons and Barnacle geese. Our study demonstrates that pathogenicity of the H5N1-2021-AB virus is lower in Pekin ducks, Eurasian wigeons and Barnacle geese compared to the H5N1-2020-C virus, whereas virus shedding was high for both viruses. After inoculation with H5N1-2021-C viral antigen expression was higher in the brain of Pekin ducks, Eurasian wigeons and Barnacle geese, which caused higher mortality compared to inoculation with H5N1-2021-AB virus. Subclinical infections occurred in Pekin ducks and Eurasian wigeons and mortality was reduced in Barnacle geese after inoculation with H5N1-2021-AB virus while H5N1-2020-C virus caused high morbidity and mortality in these species. This H5N1-2021-AB virus trait may have contributed to efficient spread of the virus in wild bird populations. Therefore, high mortality, virus shedding and long-lasting viral antigen expression found in Barnacle geese may have increased the risk for introduction into poultry.


Assuntos
Galinhas , Patos , Virus da Influenza A Subtipo H5N1 , Influenza Aviária , Eliminação de Partículas Virais , Animais , Influenza Aviária/virologia , Virus da Influenza A Subtipo H5N1/genética , Virus da Influenza A Subtipo H5N1/patogenicidade , Patos/virologia , Galinhas/virologia , Gansos/virologia , Genótipo , Doenças das Aves Domésticas/virologia
4.
Proc Biol Sci ; 291(2030): 20241448, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-39257318

RESUMO

East Asian herbivorous waterfowl intensively use farmland in spring, next to their natural habitat. Accordingly, they might have expanded their migration strategy from merely tracking the green wave of newly emerging vegetation to also incorporating the availability of post-harvest agricultural seeds (here dubbed the seed wave). However, if and how waterfowl use multiple food resources to time their seasonal migration is still unknown. We test this migration strategy using 167 spring migration tracks of five East Asian herbivorous waterfowl species and mixed-effect resource selection function models. We found that all study species arrived at their core stopover sites in the Northeast China Plain after agricultural seeds became available, extended their stay after spring vegetation emerged and arrived at their breeding sites around the emergence of vegetation. At the core stopover sites, all study species used snowmelt as a cue to track seed availability, although smaller-bodied species tended to arrive later. At the breeding sites, swans tracked the onset of vegetation emergence and geese tracked the mid- or end phases of snowmelt. Our findings suggest that waterfowl track multiple resource waves to fine-tune their migration, highlighting new opportunities for conservation.


Assuntos
Migração Animal , Anseriformes , Herbivoria , Estações do Ano , Animais , Anseriformes/fisiologia , China , Gansos/fisiologia , Ecossistema
5.
Sci Data ; 11(1): 980, 2024 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-39244605

RESUMO

Large datasets are required to develop Artificial Intelligence (AI) models in AI powered smart farming for reducing farmers' routine workload, this paper contributes the first large lion-head goose dataset GooseDetectlion, which consists of 2,660 images and 98,111 bounding box annotations. The dataset was collected with 6 cameras deployed in a goose farm in Chenghai district of Shantou city, Guangdong province, China. Images sampled from videos collected during July 9 -10 in 2022 were fully annotated by a team of fifty volunteers. Compared with another 6 well known animal datasets in literature, our dataset has higher capacity and density, which provides a challenging detection benchmark for main stream object detectors. Six state-of-the-art object detectors have been selected to be evaluated on the GooseDetectlion, which includes one two-stage anchor-based detector, three one-stage anchor-based detectors, as well as two one-stage anchor-free detectors. The results suggest that the one-stage anchor-based detector You Only Look Once version 5 (YOLO v5) achieves the best overall performance in terms of detection precision, model size and inference efficiency.


Assuntos
Inteligência Artificial , Gansos , Animais , China , Fazendas
6.
J Agric Food Chem ; 72(34): 19107-19119, 2024 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-39146474

RESUMO

Amid the growing concern for health-oriented food choices, salt reduction has received widespread attention, particularly in the exploitation of salt alternatives. Peptides with a saltiness-enhancing effect may provide an alternative method for salt reduction. The objective of this study was to isolate and extract novel peptides with salt-reducing effects by fermenting goose blood using a Lactobacillus plantarum strain. Five potential target peptides were screened by a virtual database prediction and molecular docking. Sensory evaluation and E-tongue analysis showed that five peptides (NEALQRM, GDAVKNLD, HAYNLRVD, PEMHAAFDK, and AEEKQLITGL) were identified as target peptides. Particularly, the results of E-tongue showed that GDAVKNLD can increase the saltiness intensity (2.87 ± 0.02) in the complex system. The sensory evaluation results also indicated an increase in saltiness intensity (46.67 ± 4.67 mmol/L NaCl) after adding GDAVKNLD. The results of molecular dynamics simulation indicated that five peptides have good ability to bind tightly to TMC4 receptor, thereby stimulating it to exert an active effect. And these peptides interacted with the TMC4 receptor via hydrogen bonding, hydrophobic interactions, and electrostatic interactions. This research lays a theoretical foundation for discovering novel salty/saltiness-enhancing peptides and provides meaningful contributions to efforts in salt reduction.


Assuntos
Gansos , Simulação de Acoplamento Molecular , Peptídeos , Paladar , Animais , Humanos , Peptídeos/química , Peptídeos/metabolismo , Masculino , Adulto , Feminino , Simulação de Dinâmica Molecular , Cloreto de Sódio/química , Cloreto de Sódio/metabolismo , Lactobacillus plantarum/química , Lactobacillus plantarum/metabolismo , Adulto Jovem , Ligação Proteica
7.
J Anim Sci ; 1022024 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-39158360

RESUMO

Protein kinase A (PKA) plays an important role in cellular life activities. Recently, PKA was found to bind to the inhibitor of nuclear factor-kappaB (IκB), a key protein in the nuclear factor-kappaB (NF-κB) pathway, to form a complex involved in the regulation of inflammatory response. However, the role of PKA in the anti-inflammatory of goose fatty liver is still unclear. A total of 14 healthy 70-d-old male Lander geese were randomly divided into a control group and an overfeeding group. Inflammation level was analyzed by histopathological method in the liver. The mRNA and protein abundance of PKA and tumor necrosis factor-alpha (TNFα), as well as the ubiquitination level of PKA, were detected. Moreover, goose primary hepatocytes were cotreated with glucose, harringtonine, and carbobenzoxy-l-leucyl-l-leucyl-l-leucinal (MG132). Finally, the co-immunoprecipitated samples of PKA from the control and overfeeding group were used for protein mass spectrometry. The results showed that no difference in PKA mRNA expression was observed (P > 0.05), while the PKA protein level in the overfed group was significantly reduced (P < 0.05) when compared with the control group. The ubiquitination level of PKA was higher than that of the control group in fatty liver. The mRNA expression of PKA was elevated but protein abundance was reduced in goose primary hepatocytes with 200 mmol/L glucose treatment (P < 0.05). The PKA protein abundance was dramatically reduced in hepatocytes treated with harringtonine (P < 0.01) when compared with the glucose-supplemented group. Nevertheless, MG132 tended to alleviate the inhibitory effect of harringtonine on PKA protein abundance (P = 0.081). There was no significant difference in TNFα protein level among glucose-treated groups and control (P > 0.05). Protein mass spectrometry analysis showed that 29 and 76 interacting proteins of PKA were screened in goose normal and fatty liver, respectively. Validation showed that PKA interacted with the E3 ubiquitination ligases ring finger protein 135 (RNF135) and potassium channel modulatory factor 1 (KCMF1). In summary, glucose may inhibit the inflammatory response in goose fatty liver by increasing the ubiquitination level of PKA. Additionally, RNF135 and KCMF1 may be involved in the regulation of PKA ubiquitination level as E3 ubiquitination ligases.


No obvious pathological symptoms such as inflammation were observed in fatty goose liver, suggesting that there is a unique mechanism to inhibit the development of inflammation during the goose fatty liver formation. Previous studies have shown that high glucose activated the ubiquitin­proteasome. Protein kinase A (PKA) can interact with a key protein in the nuclear factor-kappaB pathway to activate the pathway and trigger inflammatory response. To further understand how inflammation is suppressed during goose fatty liver formation. The present study showed that inflammation and PKA protein level were reduced in goose fatty liver. Meanwhile, PKA can be modified by ubiquitination in goose liver and hepatocytes. The result of the study implied that glucose deposited during goose fatty liver formation may reduce the PKA protein content by increasing the PKA ubiquitination level, thereby inhibiting the inflammatory response. Our study not only contributes to elucidate the new mechanism for suppressed inflammation in goose fatty liver but also provides a reference for the study of fatty liver in other animals.


Assuntos
Proteínas Quinases Dependentes de AMP Cíclico , Fígado Gorduroso , Gansos , Glucose , Ubiquitinação , Animais , Masculino , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Ubiquitinação/efeitos dos fármacos , Glucose/metabolismo , Fígado Gorduroso/veterinária , Fígado Gorduroso/metabolismo , Inflamação/veterinária , Doenças das Aves Domésticas , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Fator de Necrose Tumoral alfa/genética , Fígado/efeitos dos fármacos , Fígado/metabolismo
8.
Food Chem ; 461: 140919, 2024 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-39181057

RESUMO

The authenticity of salted goose products is concerning for consumers. This study describes an integrated deep-learning framework based on a generative adversarial network and combines it with data from headspace solid phase microextraction/gas chromatography-mass spectrometry, headspace gas chromatography-ion mobility spectrometry, E-nose, E-tongue, quantitative descriptive analysis, and free amino acid and 5'-nucleotide analyses to achieve reliable discrimination of four salted goose breeds. Volatile and non-volatile compounds and sensory characteristics and intelligent sensory characteristics were analyzed. A preliminary composite dataset was generated in InfoGAN and provided to several base classifiers for training. The prediction results were fused via dynamic weighting to produce an integrated model prediction. An ablation study demonstrated that ensemble learning was indispensable to improving the generalization capability of the model. The framework has an accuracy of 95%, a root mean square error (RMSE) of 0.080, a precision of 0.9450, a recall of 0.9470, and an F1-score of 0.9460.


Assuntos
Aprendizado Profundo , Cromatografia Gasosa-Espectrometria de Massas , Gansos , Paladar , Animais , Nariz Eletrônico , Compostos Orgânicos Voláteis/química , Humanos , Quimiometria , Microextração em Fase Sólida , Cruzamento
9.
Res Vet Sci ; 178: 105380, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39173243

RESUMO

Blastocystis is a common intestinal protist in humans and animals. Currently, Blastocystis infection in bar-headed geese is unknown. To understand the prevalence and distribution of Blastocystis subtypes in bar-headed geese, fecal samples were collected from 358 bar-headed geese in three regions. The total infection rate of Blastocystis in bar-headed in China was 5.9% (21/358), with 5.0% (6/120) in Aba (Ngawa) Tibetan and Qiang Autonomous Prefect, Sichuan province, 11.8% (14/119) in Maqu county, Gansu province, and 0.8% (1/119) in Caohai, Wei ning county, Guizhou province. The differences in prevalence rates by region were statistically significant. Sequences analyses showed that one known subtype (ST7, n = 16) and one potentially novel ST (n = 5) in bar-headed geese were detected in this study. This is the first report on the prevalence and subtype distribution of Blastocystis in bar-headed geese, which will improve our understanding of the epidemiology and public health implications of Blastocystis infection in wild migratory birds.


Assuntos
Infecções por Blastocystis , Blastocystis , Fezes , Gansos , Animais , Infecções por Blastocystis/epidemiologia , Infecções por Blastocystis/veterinária , Infecções por Blastocystis/parasitologia , Gansos/parasitologia , Blastocystis/isolamento & purificação , Blastocystis/classificação , China/epidemiologia , Prevalência , Fezes/parasitologia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/parasitologia , Filogenia
10.
Genes (Basel) ; 15(8)2024 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-39202396

RESUMO

The mitochondrial cytochrome c oxidase subunit I (COI) genes of six endangered goose breeds (Xupu, Yangjiang, Yan, Wuzong, Baizi, and Lingxian) were sequenced and compared to assess the genetic diversity of endangered goose breeds. By constructing phylogenetic trees and evolutionary maps of genetic relationships, the affinities and degrees of genetic variations among the six different breeds were revealed. A total of 92 polymorphic sites were detected in the 741 bp sequence of the mtDNA COI gene after shear correction, and the GC content of the processed sequence (51.11%) was higher than that of the AT content (48.89%). The polymorphic loci within the populations of five of the six breeds (Xupu, Yangjiang, Yan, Baizi, and Lingxian) were more than 10, the haplotype diversity > 0.5, and the nucleotide diversity (Pi) > 0.005, with the Baizi geese being the exception. A total of 35 haplotypes were detected based on nucleotide variation among sequences, and the goose breed haplotypes showed a central star-shaped dispersion; the FST values were -0.03781 to 0.02645, The greatest genetic differentiation (FST = 0.02645) was observed in Yan and Wuzong breeds. The most frequent genetic exchange (Nm > 15.00) was between the Wuzong and Yangjiang geese. An analysis of molecular variance showed that the population genetic variation mainly came from within the population; the base mismatch differential distribution analysis of the goose breeds and the Tajima's D and Fu's Fs neutral detection of the historical occurrence dynamics of their populations were negative (p > 0.10). The distribution curve of the base mismatches showed a multimodal peak, which indicated that the population tended to be stabilised. These results provide important genetic information for the conservation and management of endangered goose breeds and a scientific basis for the development of effective conservation strategies.


Assuntos
Complexo IV da Cadeia de Transporte de Elétrons , Espécies em Perigo de Extinção , Gansos , Haplótipos , Filogenia , Animais , Gansos/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Variação Genética , DNA Mitocondrial/genética , Cruzamento , China , Mitocôndrias/genética
11.
Poult Sci ; 103(9): 104039, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39111150

RESUMO

Low hatchability has been a persistent challenge in the goose industry. Establishing standard atlases and comprehending embryonic development patterns are essential to improving the hatching rates of goose eggs. However, comprehensive descriptions of normal atlases, embryonic development, and energy requirements in geese are lacking. In this study, a total of 120 fertile eggs from well-known large Shitou goose were incubated using 12 nesting purebred female geese. During hatching, both the temperature of the eggshells and the weight of eggs were recorded, and daily photographs of the embryos were captured to monitor their development closely. After hatching, counted the number of pores per unit area of eggshells by choosing eggs from without sperm, dead embryos, and normally hatched. Furthermore, 150 Shitou goose eggs were hatched by automatic incubator, with adjustments made based on observed normal developmental stages that incubated by female geese. The eggs were carefully opened to meticulously document embryonic morphology and create a detailed development map. Measurements were taken of the eye diameter, length of the lower beak, tarsometatarsus bone, and embryo length. Subsequently, an analysis was conducted to assess the calcium, phosphorus, crude protein, and crude fat content to study the energy requirements for embryo development. characteristics on the 7th, 15th, 23rd and 28th days of Shitou goose hatching corresponded to the 5th, 10th, 17th and 19th days of chicken egg incubation, respectively. These days were distinguished individually by "visible embryo's eye", "closure", "sealing the door", and "flashing hair". Besides, the hatch rate of the incubator reached 86.67%, and the cumulative water loss rate increased with embryo age. Notably, normally developing embryos displayed a significantly higher number of pores on the eggshell surface compared to dead embryos (P < 0.05). Additionally, embryonic body length, eyeball diameter, and lower beak length exhibited continuous growth until day 19 of incubation, while tarsometatarsus length increased steadily from days 12 to 31. Liver size measurement began on the 10th day of incubation, while both leg and chest muscles showed continuous growth from the 12th day. For energy demand, the embryo primarily relied on protein sourced from the egg yolk within the first 10 days of development. Afterward, the egg yolk provided both protein and fat for embryonic growth. In summary, this study has generated a comprehensive developmental map for Shitou goose embryos, offering valuable insights into their growth and morphological changes throughout the incubation period. This map can serve as a reference for optimizing machine incubation techniques to enhance goose egg hatching rates and provide fresh perspectives on the development of geese.


Assuntos
Desenvolvimento Embrionário , Gansos , Animais , Gansos/embriologia , Gansos/fisiologia , Gansos/crescimento & desenvolvimento , Feminino , Desenvolvimento Embrionário/fisiologia , Metabolismo Energético , Embrião não Mamífero/fisiologia , Embrião não Mamífero/embriologia , Óvulo/fisiologia , Casca de Ovo/fisiologia
12.
Front Cell Infect Microbiol ; 14: 1424212, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39165916

RESUMO

Introduction: Goose astrovirus (GAstV) is a newly emerging pathogen that is currently widespread among geese, causing visceral gout and leading to substantial gosling mortalities, posing a severe threat to the waterfowl industry. GAstV II is the predominant epidemic strain, characterized by its high morbidity and mortality rate. Consequently, there is an urgent necessity to develop an effective diagnostic approach to control the dissemination of GAstV II, particularly in clinical farms with limited laboratory resources. Methods: In this study, a novel multi-enzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) combined assay was developed. Different primers designed specific targeting a highly conserved region within the viral RdRp gene for the detection of GAstV II. Primers optimized and MIRA-LFD assay analyzed its performance regarding limits of detection, specificity, and efficiency of detection. Results: The developed MIRA amplification is conducted at a constant temperature and accomplished within 10 minutes. Subsequent naked-eye observation of the LFD strips merely takes 5 minutes. The established MIRA-LFD method exhibits high specificity, with no cross-reaction with other pathogens and attains a detection sensitivity of 1 copy/µl, which is consistent with the reverse transcription quantitative PCR (RT-qPCR) assay. Further evaluation with clinical samples indicates that the accuracy of this MIRA-LFD method correlates well with RT-qPCR for the detection of GAstV II. Conclusion: In summary, the convenience, sensitivity, and rapidity of this newly developed detection method offer a significant advantage for on-site diagnosis of GAstV II.


Assuntos
Infecções por Astroviridae , Gansos , Técnicas de Diagnóstico Molecular , Técnicas de Amplificação de Ácido Nucleico , Doenças das Aves Domésticas , Sensibilidade e Especificidade , Animais , Infecções por Astroviridae/diagnóstico , Infecções por Astroviridae/veterinária , Infecções por Astroviridae/virologia , Gansos/virologia , Técnicas de Amplificação de Ácido Nucleico/métodos , Técnicas de Diagnóstico Molecular/métodos , Doenças das Aves Domésticas/diagnóstico , Doenças das Aves Domésticas/virologia , Avastrovirus/genética , Avastrovirus/isolamento & purificação , Primers do DNA/genética , RNA Viral/genética
13.
Fa Yi Xue Za Zhi ; 40(3): 254-260, 2024 Jun 25.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-39166306

RESUMO

OBJECTIVES: To establish a rapid, accurate, and sensitive multiplex PCR detection method for the simultaneous identification of the six common edible meats (beef, lamp, chicken, pork, goose, duck), and to evaluate its application value in meat adulteration identification. METHODS: Based on complete mitochondrial genomic sequences of six species in the GenBank database, DNA sequences (cattle:16S rRNA; sheep:COX-1; chickens:Cytb; pig:COX-1; goose:NADH2; duck:16S rRNA) with intra-species conservation and inter-species specificity were screened, and species-specific primers were designed to construct a multiplex PCR detection system that can simultaneously detect the meat of six common species. The species specificity, sensitivity and reproducibility of the system were studied, and the simulated mixture sample detection was performed. RESULTS: This study successfully constructed a multiplex PCR detection system that can detect the meats of six common species simultaneously. The system was not effective in DNA amplification of non-target species. When the DNA template sizes were 0.062 5-2 ng/µL, the amplified products of all six species could be detected. The duck component was still detected when the mixing ratio of duck and beef was as low as 0.5%. CONCLUSIONS: This study constructs and establishes a multiplex PCR detection system with strong specificity, high sensitivity, and good reproducibility. It can accurately identify the components of animal origin in common edible meats and provide a simple and practical method for identifying adulteration of common edible meats and meat products in China.


Assuntos
Galinhas , Primers do DNA , Patos , Gansos , Carne , Reação em Cadeia da Polimerase Multiplex , Sensibilidade e Especificidade , Especificidade da Espécie , Animais , Reação em Cadeia da Polimerase Multiplex/métodos , Patos/genética , Reprodutibilidade dos Testes , Carne/análise , Ovinos , Suínos , Bovinos , RNA Ribossômico 16S/genética , Contaminação de Alimentos/análise
14.
Int J Mol Sci ; 25(16)2024 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-39201373

RESUMO

Low or insufficient testosterone levels caused by caponization promote fat deposition in animals. However, the molecular mechanism of fat deposition in caponized animals remains unclear. This study aimed to investigate the metabolomics and transcriptomic profiles of adipose tissues and study the effect of testosterone and leptin on the proliferation of adipocytes. We observed a significant enlargement in the areas of adipocytes in the abdominal fat tissues in capon, as well as increased luciferase activity of the serum leptin and a sharp decrease in the serum testosterone in caponized gander. Metabolomics and transcriptomic results revealed differentially expressed genes and differentially expressed metabolites with enhanced PARR signal pathway. The mRNA levels of peroxisome proliferators-activated receptor γ, fatty acid synthase, and suppressor of cytokine signaling 3 in goose primary pre-adipocytes were significantly upregulated with high leptin treatment and decreased significantly with increasing testosterone dose. Hence, reduced testosterone and increased leptin levels after caponization possibly promoted adipocytes proliferation and abdominal fat deposition by altering the expression of PPAR pathway related genes in caponized ganders. This study provides a new direction for the mechanism through which testosterone regulates the biological function of leptin and fat deposition in male animals.


Assuntos
Adipogenia , Leptina , Transdução de Sinais , Testosterona , Animais , Leptina/metabolismo , Testosterona/farmacologia , Testosterona/metabolismo , Adipogenia/efeitos dos fármacos , Masculino , Transdução de Sinais/efeitos dos fármacos , Adipócitos/metabolismo , Adipócitos/efeitos dos fármacos , Gansos/metabolismo , Receptores Ativados por Proliferador de Peroxissomo/metabolismo , Receptores Ativados por Proliferador de Peroxissomo/genética , Proliferação de Células/efeitos dos fármacos , Tecido Adiposo/metabolismo , Tecido Adiposo/efeitos dos fármacos , PPAR gama/metabolismo , PPAR gama/genética , Orquiectomia
15.
Poult Sci ; 103(9): 104065, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39043024

RESUMO

Outbreaks of short beak and dwarfism syndrome (SBDS), caused by a novel goose parvovirus (NGPV), have occurred in China since 2015. The NGPV, a single-stranded DNA virus, is thought to be vertically transmitted. However, the mechanism of NGPV immune evasion remains unclear. In this study, we investigated the impact of NGPV infection on the Cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway in duck embryonic fibroblast (DEF) cells. Our findings demonstrate that NGPV infection stimulates the mRNA expression of cGAS but results in weak IFN-ß induction. NGPV impedes the expression of IFN-ß and downstream interferon-stimulated genes, thereby reducing the secretion of IFN-ß induced by interferon-stimulating DNA (ISD) and poly (I: C). RNA-seq results show that NGPV infection downregulates interferon mRNA expression while enhancing the mRNA expression of inflammatory factors. Additionally, the results of viral protein over-expression indicate that VP1 exhibits a remarkable ability to inhibit IFN-ß expression compared to other viral proteins. Results indicated that only the intact VP1 protein could inhibit the expression of IFN-ß, while the truncated proteins VP1U and VP2 do not possess such characteristics. The immunoprecipitation experiment showed that both VP1 and VP2 could interact with IRF7 protein, while VP1U does not. In summary, our findings indicate that NGPV infection impairs the host's innate immune response by potentially modulating the expression and secretion of interferons and interferon-stimulating factors via IRF7 molecules, which are regulated by the VP1 protein.


Assuntos
Fator Regulador 7 de Interferon , Infecções por Parvoviridae , Parvovirinae , Doenças das Aves Domésticas , Transdução de Sinais , Animais , Doenças das Aves Domésticas/virologia , Doenças das Aves Domésticas/imunologia , Infecções por Parvoviridae/veterinária , Infecções por Parvoviridae/virologia , Infecções por Parvoviridae/imunologia , Fator Regulador 7 de Interferon/metabolismo , Fator Regulador 7 de Interferon/genética , Parvovirinae/genética , Parvovirinae/fisiologia , Proteínas Aviárias/genética , Proteínas Aviárias/metabolismo , Patos , Gansos , Interferon Tipo I/metabolismo , Interferon Tipo I/genética , Interferon Tipo I/imunologia
16.
Poult Sci ; 103(9): 103931, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38972281

RESUMO

Hybrid breeding has proven to enhance meat quality and is extensively utilized in goose breeding. Nevertheless, there is a paucity of research investigating the molecular mechanisms that underlie the meat quality of hybrid geese. In this study, we employed the Sichuan White Goose as the maternal line for hybridization with the Zhedong White Goose and Tianfu Meat Goose P3 line. We assessed the growth and slaughter meat quality performance of 10-wk-old hybrid offspring in comparison to Sichuan white goose purebred offspring. The results indicate that hybrid geese have significantly improved performance in growth and slaughter meat quality. Furthermore, we conducted a comprehensive analysis of the chest muscles of hybrid offspring through transcriptomics and metabolomics to unravel the effects of hybrid breeding on growth and meat quality. A total of 673 differentially expressed genes (DEGs), and 93 differentially expressed metabolites were identified. The joint analysis highlighted the significant enrichment of DEGs AMPD1, AMPD3, RRM2, ENTPD3, and the metabolite UMP in the nucleotide metabolism pathway. These findings underscore the crucial role of these genetic and metabolic factors in regulating muscle growth and meat quality in hybrid populations.


Assuntos
Gansos , Carne , Metaboloma , Transcriptoma , Animais , Gansos/genética , Gansos/crescimento & desenvolvimento , Gansos/fisiologia , Carne/análise , Hibridização Genética , Músculos Peitorais/metabolismo , Masculino , Feminino , Cruzamento
17.
Poult Sci ; 103(9): 104025, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39003791

RESUMO

Free gossypol (FG), the primary antinutritional component in cottonseed meal, can adversely affect the growth and health of poultry. Although younger geese are particularly sensitive to FG, the precise effects of FG on geese remain elusive. This study aimed to investigate the effects of gossypol acetate (GA), a form of FG, on the growth, serum biochemical parameters, and intestinal health of goslings. Seventy-two healthy male goslings, aged 7-day-old with similar body weight (BW), were randomly divided into 3 groups: a control group and 2 GA-treated groups (GA25 and GA50), which were orally administered GA (25 and 50 mg/kg BW) daily for 14 d. The results showed that oral administration of GA significantly suppressed BW, altered serum parameters, and impaired intestinal health in a dose- and time-dependent manner. Specifically, GA adversely affected intestinal morphology, induced oxidative stress, and inflammation, diminished immune function, and increased intestinal permeability and apoptosis of intestinal cells, consequently impairing nutrient absorption and utilization of goslings. Overall, these data indicate that GA adversely affects the growth, serum parameters, and intestinal health of goslings, providing valuable information further to understand the toxic effects of gossypol on goslings.


Assuntos
Gansos , Gossipol , Intestinos , Animais , Gossipol/farmacologia , Gossipol/administração & dosagem , Masculino , Gansos/crescimento & desenvolvimento , Intestinos/efeitos dos fármacos , Ração Animal/análise , Distribuição Aleatória , Dieta/veterinária , Relação Dose-Resposta a Droga , Estresse Oxidativo/efeitos dos fármacos , Análise Química do Sangue/veterinária
18.
Sci Data ; 11(1): 741, 2024 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-38972874

RESUMO

Our study presents the assembly of a high-quality Taihu goose genome at the Telomere-to-Telomere (T2T) level. By employing advanced sequencing technologies, including Pacific Biosciences HiFi reads, Oxford Nanopore long reads, Illumina short reads, and chromatin conformation capture (Hi-C), we achieved an exceptional assembly. The T2T assembly encompasses a total length of 1,197,991,206 bp, with contigs N50 reaching 33,928,929 bp and scaffold N50 attaining 81,007,908 bp. It consists of 73 scaffolds, including 38 autosomes and one pair of Z/W sex chromosomes. Importantly, 33 autosomes were assembled without any gap, resulting in a contiguous representation. Furthermore, gene annotation efforts identified 34,898 genes, including 436,162 RNA transcripts, encompassing 806,158 exons, 743,910 introns, 651,148 coding sequences (CDS), and 135,622 untranslated regions (UTR). The T2T-level chromosome-scale goose genome assembly provides a vital foundation for future genetic improvement and understanding the genetic mechanisms underlying important traits in geese.


Assuntos
Gansos , Genoma , Telômero , Animais , Gansos/genética , Telômero/genética , Anotação de Sequência Molecular
19.
Microb Pathog ; 194: 106825, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39074517

RESUMO

Short beak and dwarfism syndrome (SBDS) is attributed to Novel Goose Parvovirus (NGPV), which has inflicted significant economic losses on farming in China. Despite its significant impact, limited research has been conducted on the pathogenesis of this disease. The SD strain, a parvovirus variant isolated from ducks in Shandong province, was identified and characterized in our study. Phylogenetic analysis and sequence comparisons confirmed the classification of the SD strain as a member of NGPV. Based on this information, we established an animal model of SBDS by inoculating Cherry Valley ducks with the SD strain. Our findings indicate that infection with the SD strain leads to a reduction in body weight, beak length, width, and tibia length. Notably, significant histopathological alterations were observed in the thymus, spleen, and intestine of the infected ducks. Furthermore, the SD strain induces bone disorders and inflammatory responses. To evaluate the impact of NGPV on intestinal homeostasis, we performed 16S rDNA sequencing and gas chromatography to analyze the composition of intestinal flora and levels of short-chain fatty acids (SCFAs) in the cecal contents. Our findings revealed that SD strain infection induces dysbiosis in cecal microbial and a decrease in SCFAs production. Subsequent analysis revealed a significant correlation between bacterial genera and the clinical symptoms in NGPV SD infected ducks. Our research providing novel insights into clinical pathology of NGPV in ducks and providing a foundation for the research of NGPV treatment targeting gut microbiota.


Assuntos
Patos , Infecções por Parvoviridae , Filogenia , Doenças das Aves Domésticas , Animais , Patos/virologia , Infecções por Parvoviridae/veterinária , Infecções por Parvoviridae/virologia , Infecções por Parvoviridae/patologia , Doenças das Aves Domésticas/virologia , Doenças das Aves Domésticas/patologia , China , Parvovirinae/genética , Parvovirinae/isolamento & purificação , Parvovirinae/patogenicidade , Microbioma Gastrointestinal , Intestinos/patologia , Intestinos/virologia , RNA Ribossômico 16S/genética , Modelos Animais de Doenças , Disbiose/virologia , Disbiose/veterinária , Ácidos Graxos Voláteis/metabolismo , Gansos/virologia , Baço/patologia , Baço/virologia , Bico/virologia , Bico/patologia
20.
Microb Pathog ; 194: 106829, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39084310

RESUMO

Goose astroviruses (GAstVs) are important pathogens which can cause gout in goslings leading to huge economic losses for the goose farming industry in China. In 2023, an infectious disease characterized by visceral gout broke out in commercial goose farms in Guangxi and Guangdong provinces of China. In this study, two GAstV strains of GXNN and GDCS were successfully isolated from these two disease-ridden goose farms. The complete genomic lengths of these two strains were 7166 bp, and phylogenetic analysis showed that they were both GAstV-2 subtypes. The 3-dimensional structures of the capsid protein were predicted and six characteristic mutation sites at amino acid positions 60, 61, 228, 229, 456 and 523 were found within the strong antigenic regions. A recombination event occurred at 6833-7070 nt between the GAstV TZ03 and Turkey astrovirus CA/00 and this was detected in both the GXNN and GDCS strains. Another recombinant event occurred at 63-2747 nt between the GAstV XT1 and GAstV SDPY and this was detected in the GDCS strain. When 1-day-old goslings were infected with the novel GXNN and GDCS strains, they showed severe visceral gout. This was accompanied by enlarged spleens, liver hemorrhages and urate deposits in the kidneys and ureters and their blood urea nitrogen levels were significantly elevated. The mortality rates of the GXNN- and GDCS-infected groups were pathogenically high at 80 % and 60 %, respectively. These results will promote our understanding of the evolution and epidemic potential of GAstVs in China.


Assuntos
Infecções por Astroviridae , Proteínas do Capsídeo , Gansos , Genoma Viral , Gota , Filogenia , Doenças das Aves Domésticas , Animais , Gansos/virologia , China , Infecções por Astroviridae/veterinária , Infecções por Astroviridae/virologia , Doenças das Aves Domésticas/virologia , Doenças das Aves Domésticas/patologia , Gota/virologia , Gota/veterinária , Gota/patologia , Proteínas do Capsídeo/genética , Avastrovirus/genética , Avastrovirus/patogenicidade , Avastrovirus/isolamento & purificação , Avastrovirus/classificação , Virulência , Astroviridae/genética , Astroviridae/isolamento & purificação , Astroviridae/patogenicidade
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