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1.
Mycoses ; 67(9): e13785, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39245647

RESUMO

Antifungal-resistant dermatophytes (ARD) infection is a hotspot issue in clinical microbiology and the dermatology field. Trichophyton indotineae as the dominant species of dermatophyte with terbinafine-resistance or multidrug resistance, is easy to be missed detection clinically, which brings severe challenges to diagnosis and treatment. ARD infection cases have emerged in China, and it predicts a risk of transmission among human. Based on the existing medical evidence and research data, the Mycology Group of Combination of Traditional and Western Medicine Dermatology and Chinese Antifungal⁃Resistant Dermatophytoses Expert Consensus Group organized experts to make consensus on the management of the infection. Here, the consensus formulated diagnosis and treatment recommendations, to raise attention to dermatophytes drug resistance problem, and expect to provide reference information for the clinical diagnosis, treatment, prevention and control.


Assuntos
Antifúngicos , Consenso , Farmacorresistência Fúngica , Tinha , Humanos , Antifúngicos/uso terapêutico , Antifúngicos/farmacologia , Arthrodermataceae/efeitos dos fármacos , China , Tinha/tratamento farmacológico , Tinha/microbiologia , Tinha/diagnóstico , Trichophyton/efeitos dos fármacos , Trichophyton/isolamento & purificação
3.
Med Mycol ; 62(8)2024 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-39174488

RESUMO

The Trichophyton mentagrophytes complex comprises a group of dermatophyte fungi responsible for various dermatological infections. The increasing drug resistance of this species complex, especially terbinafine resistance of Trichophyton indotineae, is a major concern in dermatologist practice. This study provides a comprehensive analysis of T. mentagrophytes complex strains isolated from patients in Hue City, Vietnam, focusing on their phenotypic and genetic characteristics, antifungal susceptibility profiles, and molecular epidemiology. Keratinophilic fungi from dermatophytosis culture samples were identified morphologically and phenotypically, with species and genotypes confirmed by internal transcribed spacer sequencing and phylogenetic analysis. Antifungal susceptibility testing was carried out to evaluate their susceptibility to itraconazole, voriconazole, and terbinafine. The 24% (n = 27/114) of superficial mycoses were phenotypically attributed to T. mentagrophytes complex isolates. Trichophyton interdigitale, mainly genotype II*, was predominant (44.4%), followed by T. mentagrophytes genotype III* (22.2%), T. indotineae (14.8%), T. tonsurans (11.2%), and T. mentagrophytes (7.4%). While all isolates were susceptible to itraconazole and voriconazole, half of T. indotineae isolates exhibited resistance to terbinafine, linked to the Phe397Leu mutation in the SQLE protein. This study highlighted the presence of terbinafine-resistant T. indotineae isolates in Vietnam, emphasizing the need to investigate dermatophyte drug resistance and implement effective measures in clinical practice.


Species diversity within the Trichophyton mentagrophytes complex isolated from dermatophytosis in Hue City, Vietnam, was observed. Terbinafine-resistant T. indotineae isolates were detected for the first time in Vietnam, emphasizing the importance of implementing antifungal susceptibility testing to effectively manage and prevent the spread of resistant isolates.


Assuntos
Antifúngicos , Farmacorresistência Fúngica , Genótipo , Testes de Sensibilidade Microbiana , Filogenia , Terbinafina , Tinha , Humanos , Vietnã , Antifúngicos/farmacologia , Terbinafina/farmacologia , Tinha/microbiologia , Arthrodermataceae/efeitos dos fármacos , Arthrodermataceae/genética , Arthrodermataceae/classificação , Arthrodermataceae/isolamento & purificação , Masculino , Análise de Sequência de DNA , Itraconazol/farmacologia , DNA Espaçador Ribossômico/genética , Feminino , Pessoa de Meia-Idade , DNA Fúngico/genética , Epidemiologia Molecular , Adulto , Trichophyton
4.
Int J Mol Sci ; 25(15)2024 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-39125871

RESUMO

Caffeine affords several beneficial effects on human health, acting as an antioxidant, anti-inflammatory agent, and analgesic. Caffeine is widely used in cosmetics, but its antimicrobial activity has been scarcely explored, namely against skin infection agents. Dermatophytes are the most common fungal agents of human infection, mainly of skin infections. This work describes the in vitro effect of caffeine during keratinocyte infection by Trichophyton mentagrophytes, one of the most common dermatophytes. The results show that caffeine was endowed with antidermatophytic activity with a MIC, determined following the EUCAST standards, of 8 mM. Caffeine triggered a modification of the levels of two major components of the fungal cell wall, ß-(1,3)-glucan and chitin. Caffeine also disturbed the ultrastructure of the fungal cells, particularly the cell wall surface and mitochondria, and autophagic-like structures were observed. During dermatophyte-human keratinocyte interactions, caffeine prevented the loss of viability of keratinocytes and delayed spore germination. Overall, this indicates that caffeine can act as a therapeutic and prophylactic agent for dermatophytosis.


Assuntos
Antifúngicos , Arthrodermataceae , Cafeína , Queratinócitos , Cafeína/farmacologia , Queratinócitos/efeitos dos fármacos , Queratinócitos/microbiologia , Humanos , Antifúngicos/farmacologia , Arthrodermataceae/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Parede Celular/efeitos dos fármacos , Tinha/tratamento farmacológico , Tinha/microbiologia , Quitina/farmacologia , Quitina/química
5.
Med Mycol ; 62(9)2024 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-39174486

RESUMO

This study analyzed the prevalence and antifungal susceptibility of superficial fungal infections in 295 cases from 2019 to 2020 at a dermatology clinic. Dermatophytes were the predominant pathogens (69.5%), including Trichophytonrubrum, T. interdigitale, Microsporum canis, et al., followed by Candida spp. (29.5%), including Candidaalbicans, Ca. parapsilosis, and Ca. glabrata. The most common infections were onychomycosis (36.3%), tinea cruris (30.5%), and tinea corporis (18.6%). The distribution of SFI types showed variations based on gender, age, and season. Common antifungal agents, including terbinafine, voriconazole, ciclopiroxamine, amphotericin B, itraconazole, and ketoconazole have exhibited low minimum inhibitory concentrations against dermatophytes, especially terbinafine, which has been potent against superficial fungal infections caused by dermatophytes in the local area. Candida spp. strains were generally susceptible or classified as wild-type to 5-flucytosine and amphotericin B, with 92.0% being wild-type for itraconazole. However, resistance to fluconazole and voriconazole was observed in a small percentage of Ca. albicans and Ca. parapsilosis strains. The emergence of drug-resistant Candida underscores the importance of prudent antifungal use and continuous surveillance.


Our study analyzed 295 cases of superficial fungal infections in Taiyuan, located in Northern China. Dermatophytes and Candida spp. were primary pathogens, with varied susceptibilities to antifungals. Results deepen our understanding, emphasizing prudent drug use and surveillance.


Assuntos
Antifúngicos , Arthrodermataceae , Candida , Farmacorresistência Fúngica , Testes de Sensibilidade Microbiana , Centros de Atenção Terciária , Humanos , Antifúngicos/farmacologia , China/epidemiologia , Centros de Atenção Terciária/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Feminino , Adulto , Arthrodermataceae/efeitos dos fármacos , Arthrodermataceae/genética , Arthrodermataceae/classificação , Arthrodermataceae/isolamento & purificação , Adolescente , Adulto Jovem , Criança , Idoso , Candida/efeitos dos fármacos , Candida/classificação , Candida/isolamento & purificação , Candida/genética , Pré-Escolar , Dermatomicoses/microbiologia , Dermatomicoses/epidemiologia , Epidemiologia Molecular , Prevalência , Lactente , Idoso de 80 Anos ou mais
6.
Tunis Med ; 102(8): 447-451, 2024 Aug 05.
Artigo em Francês | MEDLINE | ID: mdl-39129570

RESUMO

BACKGROUND: dermatophytoses are a current fungal infection, caused by keratinophilic fungi (dermatophytes) able to invade the nails, hair and skin of humans and animals. AIM: the aim of this study was to establish the epidemiological and mycological profile of dermatophytes isolated in the parasitology-mycology laboratory of the Mohammed VI University Hospital in Oujda. METHODS: this is a 48-month retrospective study from January 2019 to December 2022.The study includes samples taken or sent to our parasitology-mycology laboratory for mycological study. A direct examination and culture were performed on each biological specimen. Species identification was based on macroscopic and microscopic colony criteria. RESULTS: in the present report we reviewed 950 mycological samples. Dermatophytes were isolated in 505 (53.15%) cases. The most common infections were tinea unguium (n=353; 69.90%), followed by tinea corporis (n=123; 5.74%) and tinea capitis (n=29; 5.98%). Trichophyton rubrum was the most frequently incriminated species. CONCLUSION: dermatophytes are the most frequent mycoses in humans. They are generally benign and often develop in a chronic and frequently recurrent pattern. Mycological examination is essential. It confirms the fungal origin and isolates the species responsible, in order to identify the source of contamination and implement an appropriate treatment.


Assuntos
Arthrodermataceae , Hospitais Universitários , Humanos , Estudos Retrospectivos , Arthrodermataceae/isolamento & purificação , Feminino , Masculino , Adulto , Pessoa de Meia-Idade , Criança , Adolescente , Adulto Jovem , Idoso , Pré-Escolar , Tinha/epidemiologia , Tinha/microbiologia , Tinha/diagnóstico , Dermatomicoses/epidemiologia , Dermatomicoses/microbiologia , Dermatomicoses/diagnóstico , Marrocos/epidemiologia , Lactente , Micologia/métodos , Idoso de 80 Anos ou mais
7.
J Nat Med ; 78(4): 929-951, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39103726

RESUMO

Fungi, such as Trichophyton rubrum (T. rubrum) and Microsporum canis Bodin Anamorph (M. canis Bodin Anamorph) are the main pathogens of dermatophysis. According to ancient books records, Rumex japonicus Houtt. (RJH) has a miraculous effect on the treatment of dermatophysis. To reveal the anti-fungi (T. rubrum and M. canis Bodin Anamorph) components and its mechanism of the Rumex japonicus Houtt. The vinegar extraction and alcohol precipitation, HPLC and nuclear magnetic resonance spectroscopy (NMR) were employed for analyzing the chemical compositions of RJH; in vitro anti-fungal experiment was investigated including test the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC), spore germination rate, nucleic acid, protein leakage rate, biofilm structure, and the mechanism of anti-fungal and anti-fungal biofilms in RJH. Seven anthraquinones and their glycoside compounds were obtained in this study respectively, such as chrysophanol, physcion, aloe-emodin, emodin, rhein, emodin-8-O-ß-D-glucoside and chrysophanol-8-O-ß-D-glucoside. In vitro anti-fungal experiment results showed that RJH extracts have good anti-fungal activity for dermatophytic fungi. Among them, the MIC of the rhein, emodin and aloe-emodin against T. rubrum are 1.9 µg/ml, 3.9 µg/ml and 15.6 µg/ml, respectively; the MIC of emodin and aloe-emodin against M. canis Bodin Anamorph are 7.8 µg/ml and 62.5 µg/ml, respectively. In addition, its active components can inhibit fungal spore germination and the formation of bud tube, change cell membrane permeability, prevent hyphal growth, destroy biofilm structure, and down-regulate the expression of agglutinin-like sequence family 1 of the adhesion phase of biofilm growth. The study shows that RJH play a fungicidal role.


Assuntos
Antraquinonas , Antifúngicos , Biofilmes , Glicosídeos , Testes de Sensibilidade Microbiana , Microsporum , Rumex , Antraquinonas/farmacologia , Antraquinonas/química , Antraquinonas/isolamento & purificação , Rumex/química , Antifúngicos/farmacologia , Antifúngicos/química , Antifúngicos/isolamento & purificação , Glicosídeos/farmacologia , Glicosídeos/química , Microsporum/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Emodina/farmacologia , Emodina/química , Emodina/análogos & derivados , Arthrodermataceae
8.
Emerg Infect Dis ; 30(9): 1978-1980, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39174019

RESUMO

Antifungal-resistant dermatophyte infections have recently emerged as a global public health concern. A survey of US infectious diseases specialists found that only 65% had heard of this issue and just 39% knew how to obtain testing to determine resistance. Increased clinician awareness and access to testing for antifungal-resistant dermatophytosis are needed.


Assuntos
Antifúngicos , Farmacorresistência Fúngica , Tinha , Humanos , Antifúngicos/uso terapêutico , Antifúngicos/farmacologia , Estados Unidos/epidemiologia , Tinha/microbiologia , Tinha/epidemiologia , Tinha/tratamento farmacológico , Inquéritos e Questionários , Arthrodermataceae/efeitos dos fármacos , Testes de Sensibilidade Microbiana
9.
Am J Trop Med Hyg ; 111(3): 575-577, 2024 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-38981494

RESUMO

Over the past few years, a recalcitrant dermatophytosis has been observed on the Indian subcontinent, including Sri Lanka, which has caused a major public health issue in the region. An emerging species, Trichophyton indotineae, first described as Trichophyton mentagrophytes ITS genotype VIII, is thought to be responsible for this fast-spreading, mostly terbinafine-resistant dermatophytosis. Recalcitrant dermatophytosis is a challenge to dermatologists, and knowing the causative species and antifungal sensitivity in the earlier stage of management would be invaluable. We report a case series of patients with dermatophytosis caused by T. indotineae in Sri Lanka. This is the first detection of this highly terbinafine-resistant strain in Sri Lanka, and existence of this species should be taken seriously by dermatologists and healthcare policymakers for better management of tinea infections and antifungal stewardship in the country.


Assuntos
Antifúngicos , Genótipo , Terbinafina , Tinha , Humanos , Tinha/tratamento farmacológico , Tinha/microbiologia , Sri Lanka , Antifúngicos/uso terapêutico , Antifúngicos/farmacologia , Masculino , Feminino , Terbinafina/uso terapêutico , Adulto , Pessoa de Meia-Idade , Farmacorresistência Fúngica , Trichophyton/genética , Trichophyton/isolamento & purificação , Trichophyton/efeitos dos fármacos , Trichophyton/classificação , Arthrodermataceae/genética , Arthrodermataceae/isolamento & purificação , Arthrodermataceae/efeitos dos fármacos , Arthrodermataceae/classificação
10.
J Mycol Med ; 34(3): 101498, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38986424

RESUMO

Dermatophyte infections frequently pose diagnostic challenges, especially when occurring alongside ichthyosis, a genetic skin disorder characterized by dry, thickened, scaly skin. This case series outlines three cases where dermatophyte infections overlapped with ichthyosis, emphasizing the complexities in clinical identification and differential diagnosis. Atypical clinical presentations in these cases led to initial misdiagnoses. Ichthyosis, a genetic skin disorder characterized by thickened and scaly skin, creates an environment conducive to dermatophyte settlement, complicating the diagnostic process. The cases highlight the importance of considering fungal infections, even when clinical features deviate from the expected course. A vigilant diagnostic approach, including mycological examinations, is crucial for accurate identification and timely management.


Assuntos
Dermatomicoses , Ictiose , Humanos , Masculino , Ictiose/microbiologia , Ictiose/complicações , Ictiose/diagnóstico , Dermatomicoses/microbiologia , Dermatomicoses/diagnóstico , Feminino , Diagnóstico Diferencial , Adulto , Coinfecção/microbiologia , Coinfecção/diagnóstico , Pessoa de Meia-Idade , Arthrodermataceae/isolamento & purificação , Arthrodermataceae/classificação , Antifúngicos/uso terapêutico
11.
J Mycol Med ; 34(3): 101496, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38986423

RESUMO

BACKGROUND: Despite changes in the epidemiology of dermatophyte infections, the incidence of fungal infections associated with Trichophyton species still remains high among dogs and cats. The objective of the present study was to isolate and characterize dermatophytes from dogs and cats in Iran. METHOD: From December 2022 to May 2023, skin and hair samples were collected from symptomatic and asymptomatic cats and dogs in Mazandaran, a northern province of Iran. The samples were then inoculated into Mycosel™ Agar. Dermatophyte isolates were identified by sequencing the internal transcribed spacer region. Antifungal susceptibility tests were conducted using the Clinical and Laboratory Standards Institute (CLSI-M38-A3). RESULT: Of the 250 samples collected (from 200 dogs and 50 cats), 20 (from 19 dogs and one cat) (8.0 %) were positive for dermatophyte growth. Based on sequence and phylogenetic analysis, all isolates belonged to T. mentagrophytes II*. Of these positive samples, 14 (70.0 %), 3 (15.0 %), 2 (10.0 %), and 1 (2.0 %) were isolated from asymptomatic stray dogs, symptomatic stray dogs, symptomatic domestic dogs, and symptomatic cats, respectively. Luliconazole and terbinafine displayed potent activity against all T. mentagrophytes isolates, with Minimum inhibitory concentration (MIC) values of 0.016 µg/ml. Miconazole and griseofulvin demonstrated higher MIC (1 and 8 µg/ml). CONCLUSION: The present study indicated that T. mentagrophytes II* asymptomatic carriage is frequent in stray dogs in Iran. The potential risk to public health needs to be evaluated However, T. mentagrophytes genotype VIII, considered as an endemic and emerging human pathogenic clone in several countries, was not detected during the present survey.


Assuntos
Antifúngicos , Arthrodermataceae , Doenças do Gato , Doenças do Cão , Testes de Sensibilidade Microbiana , Filogenia , Tinha , Cães/microbiologia , Gatos/microbiologia , Animais , Irã (Geográfico)/epidemiologia , Doenças do Cão/microbiologia , Doenças do Cão/epidemiologia , Doenças do Gato/microbiologia , Doenças do Gato/epidemiologia , Antifúngicos/farmacologia , Tinha/microbiologia , Tinha/epidemiologia , Tinha/veterinária , Arthrodermataceae/isolamento & purificação , Arthrodermataceae/efeitos dos fármacos , Arthrodermataceae/genética , Arthrodermataceae/classificação , Masculino , Feminino , Cabelo/microbiologia , Infecções Assintomáticas/epidemiologia
12.
Braz J Biol ; 84: e275974, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39046047

RESUMO

The therapeutic potential of medicinal plants is known as an alternative in treatment of human affections; in effect, the conventional application of these medicinal sources has several limitations like low bioavailability, solubility and stability, which affect its pharmacological efficacy. In recent decades, extraordinary advances have been made in new drug delivery systems using nanocarriers. This work consisted in determining the in vitro antifungal activity of the methanolic extract of Euphorbia tirucalli formulated in polymeric nanoparticles. The antifungal activity was determined by the microdilution method in 96-well microplates, applying nanoparticles loaded with plant extract (NP-Ext) obtained by nanoprecipitation on clinical isolates of Trichophyton rubrum and T. interdigitalis. Regarding the nanoparticles, the lots used did not present significant differences in their physicochemical characteristics, with a size of 91.885 ± 1.621nm, polydispersity index of 0.152 ± 0.025 and Z-potential of -6.047 ± 0.987. The quantification of the extract in the polymeric matrix was determined by infrared spectroscopy (FTIR), where an efficiency and encapsulation percentage of 22.15 ± 0.82 and 2.95 ± 0.11, respectively, were obtained. The in vitro antifungal activity of the crude and formulated extract was obtained calculating the Minimum Inhibitory Concentration (MIC) of each one; a MIC of 125 µg/mL was obtained against T. rubrum and T. interdigitalis with the crude extract, while a MIC value of 55.55 and 0.1 µg/mL was obtained with NP-Ext, respectively, against these same. Conclusions: biological activity is closely linked to the phytochemical profile of the extract; while the improvement of said potential with the NP-Ext with the dosage form was directly related to the physicochemical characteristics of the nanocarrier.


Assuntos
Antifúngicos , Euphorbia , Testes de Sensibilidade Microbiana , Nanopartículas , Extratos Vegetais , Euphorbia/química , Antifúngicos/farmacologia , Antifúngicos/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Nanopartículas/química , Espectroscopia de Infravermelho com Transformada de Fourier , Trichophyton/efeitos dos fármacos , Polímeros/farmacologia , Polímeros/química , Arthrodermataceae
13.
Eur J Dermatol ; 34(3): 260-266, 2024 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-39015959

RESUMO

Onychomycosis, a fungal nail infection, is primarily caused by dermatophytes, yeasts, and non-dermatophyte moulds (NDMs). The incidence of this disease and the predominance of specific pathogens vary across different regions and evolve. This study aimed to elucidate the epidemiology of onychomycosis and the pattern of causative pathogens in Beijing, and to ascertain the in vitro antifungal susceptibility profiles of Trichophyton rubrum against itraconazole (ITR), terbinafine (TER), and fluconazole (FLU). Involving 245 patients of onychomycosis with positive fungal culture results, the study implemented internal transcribed spacer (ITS) sequencing of ribosomal DNA (rDNA) on all collected samples. The mean age of the participants was 37.93 ± 13.73 years, with a male-to-female ratio of 1.53:1. The prevalence of toenail infections was significantly higher than that of fingernails. Distal and lateral subungual onychomycosis (DLSO) were the most frequent clinical classifications. PCR results indicated that dermatophytes were the most prevalent pathogens, followed by yeasts and NDMs, among which T. rubrum was the most dominant dermatophyte. TER demonstrated high sensitivity to T. rubrum. However, in clinical settings, some patients with onychomycosis exhibit a poor response to TER treatment. The relationship between in vitro antifungal sensitivity and clinical effectiveness is complex, and understanding the link between in vitro MIC values and clinical efficacy requires further investigation.


Assuntos
Antifúngicos , Fluconazol , Dermatoses do Pé , Itraconazol , Testes de Sensibilidade Microbiana , Onicomicose , Terbinafina , Humanos , Onicomicose/microbiologia , Onicomicose/tratamento farmacológico , Onicomicose/epidemiologia , Masculino , Antifúngicos/farmacologia , Antifúngicos/uso terapêutico , Feminino , Adulto , Pessoa de Meia-Idade , Terbinafina/farmacologia , Terbinafina/uso terapêutico , Dermatoses do Pé/microbiologia , Dermatoses do Pé/tratamento farmacológico , Itraconazol/farmacologia , Itraconazol/uso terapêutico , Fluconazol/farmacologia , Arthrodermataceae/efeitos dos fármacos , Adulto Jovem , Dermatoses da Mão/microbiologia , Dermatoses da Mão/tratamento farmacológico , Dermatoses da Mão/epidemiologia , China/epidemiologia , Prevalência , Trichophyton/efeitos dos fármacos , Idoso , Adolescente
14.
PLoS One ; 19(7): e0306829, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38980893

RESUMO

An unambiguous identification of dermatophytes causing dermatophytoses is necessary for accurate clinical diagnosis and epidemiological implications. In the current taxonomy of the Arthrodermataceae, the etiological agents of dermatophytoses consist of seven genera and members of the genera Trichophyton are the most prevalent etiological agents at present. The genera Trichophyton consists of 16 species that are grouped as clades, but the species borderlines are not clearly delimited. The aim of the present study was to determine the discriminative power of subtilisin gene variants (SUB1-SUB12) in family Arthrodermataceae, particularly in Trichophyton. Partial and complete reads from 288 subtilisin gene sequences of 12 species were retrieved and a stringent filtering following two different approaches for analysis (probability of correct identification (PCI) and gene gap analysis) conducted to determine the uniqueness of the subtilisin gene subtypes. SUB1 with mean PCI value of 60% was the most suitable subtilisin subtype for specific detection of T.rubrum complex, however this subtype is not reported in members of T. mentagrophytes complex which is one of the most prevalent etiological agent at present. Hence, SUB7 with 40% PCI value was selected for testing its discriminative power in Trichophyton species. SUB7 specific PCR based detection of dermatophytes was tested for sensitivity and specificity. Sequences of SUB7 from 42 isolates and comparison with the ITS region showed that differences within the subtilisin gene can further be used to differentiate members of the T. mentagrophytes complex. Further, subtilisin cannot be used for the differentiation of T. benhamiae complex since all SUB subtypes show low PCI scores. Studies on the efficiency and limitations of the subtilisin gene as a diagnostic tool are currently limited. Our study provides information that will guide researchers in considering this gene for identifying dermatophytes causing dermatophytoses in human and animals.


Assuntos
Arthrodermataceae , Arthrodermataceae/genética , Arthrodermataceae/isolamento & purificação , Humanos , Tinha/microbiologia , Tinha/diagnóstico , Subtilisina/genética , Trichophyton/genética , Trichophyton/isolamento & purificação , Filogenia , Proteínas Fúngicas/genética
15.
Mycoses ; 67(7): e13768, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-39036952

RESUMO

BACKGROUND: There is a concerning rise in antifungal-resistant dermatophytosis globally, with resistance to terbinafine conferred by point mutations in the squalene epoxidase (SQLE) gene. OBJECTIVES: Report changes in the prevalence and profile of SQLE mutations in onychomycosis patients in the United States. METHODS: A longitudinal cohort study of toenail samples was collected from suspected onychomycosis patients over an 18-month period from 2022 to 2023. Samples were submitted from across the United States and subjected to multiplex real-time polymerase chain reactions for dermatophyte detection, with further screening of SQLE mutations at four known hotspots (393Leu, 397Phe, 415Phe and 440His). RESULTS: A total of 62,056 samples were submitted (mean age: 57.5 years; female: 60.4%). Dermatophytes were detected in 38.5% of samples, primarily Trichophyton rubrum complex (83.6%) and T. mentagrophytes complex (10.7%). A survey of SQLE mutations was carried out in 22,610 dermatophyte samples; there was a significant increase in the prevalence of SQLE mutations between the first quarter of 2022 and the second quarter of 2023 (29.0 to 61.9 per 1000 persons). The Phe397Leu substitution was the predominant mutation; Phe415Ser and His440Tyr have also emerged which were previously reported as minor mutations in skin samples. The temporal change in mutation rates can be primarily attributed to the Phe415Ser substitution. Samples from elderly patients (>70 years) are more likely to be infected with the T. mentagrophytes complex including strains harbouring the Phe415Ser substitution. CONCLUSION: The prevalence of SQLE mutations among onychomycosis patients with Trichophyton infections may be underestimated. Older individuals may have a higher risk.


Assuntos
Antifúngicos , Arthrodermataceae , Farmacorresistência Fúngica , Onicomicose , Esqualeno Mono-Oxigenase , Terbinafina , Humanos , Onicomicose/microbiologia , Onicomicose/epidemiologia , Onicomicose/tratamento farmacológico , Esqualeno Mono-Oxigenase/genética , Feminino , Pessoa de Meia-Idade , Masculino , Terbinafina/farmacologia , Terbinafina/uso terapêutico , Farmacorresistência Fúngica/genética , Estados Unidos/epidemiologia , Antifúngicos/farmacologia , Antifúngicos/uso terapêutico , Estudos Longitudinais , Idoso , Arthrodermataceae/genética , Arthrodermataceae/efeitos dos fármacos , Adulto , Mutação , Estudos de Coortes , Trichophyton/genética , Trichophyton/efeitos dos fármacos , Adulto Jovem , Prevalência , Mutação Puntual , Idoso de 80 Anos ou mais , Adolescente , Unhas/microbiologia
16.
AAPS PharmSciTech ; 25(6): 156, 2024 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-38981986

RESUMO

Commercial topical formulations containing itraconazole (poorly water soluble), for mycotic infections, have poor penetration to infection sites beneath the nails and skin thereby necessitating oral administration. To improve penetration, colloidal solutions of itraconazole (G1-G4) containing Poloxamer 188, tween 80, ethanol, and propylene glycol were prepared and incorporated into HFA-134-containing sprays. Formulations were characterized using particle size, drug content, and Fourier-transform infrared spectroscopy (FTIR). In vitro permeation studies were performed using Franz diffusion cells for 8 h. Antimycotic activity on Candida albicans and Trichophyton rubrum was performed using broth micro-dilution and flow cytometry, while cytotoxicity was tested on HaCaT cell lines. Particle size ranged from 39.35-116.80 nm. FTIR and drug content revealed that G1 was the most stable formulation (optimized formulation). In vitro release over 2 h was 45% for G1 and 34% for the cream. There was a twofold increase in skin permeation, fivefold intradermal retention, and a sevenfold increase in nail penetration of G1 over the cream. Minimum fungicidal concentrations (MFC) against C. albicans were 0.156 and 0.313 µg/mL for G1 and cream, respectively. The formulations showed optimum killing kinetics after 48 h. MFC values against T. rubrum were 0.312 and 0.625 µg/mL for the G1 and cream, respectively. Transmission electron microscopy revealed organelle destruction and cell leakage for G1 in both organisms and penetration of keratin layers to destroy T. rubrum. Cytotoxicity evaluation of G1 showed relative safety for skin cells. The G1 formulation showed superior skin permeation, nail penetration, and fungicidal activity compared with the cream formulation.


Assuntos
Antifúngicos , Candida albicans , Coloides , Itraconazol , Antifúngicos/farmacologia , Antifúngicos/administração & dosagem , Candida albicans/efeitos dos fármacos , Itraconazol/farmacologia , Itraconazol/administração & dosagem , Itraconazol/química , Humanos , Animais , Trichophyton/efeitos dos fármacos , Testes de Sensibilidade Microbiana/métodos , Química Farmacêutica/métodos , Tamanho da Partícula , Pele/metabolismo , Pele/efeitos dos fármacos , Pele/microbiologia , Absorção Cutânea/efeitos dos fármacos , Linhagem Celular , Células HaCaT , Unhas/efeitos dos fármacos , Unhas/microbiologia , Unhas/metabolismo , Arthrodermataceae
17.
J Mycol Med ; 34(3): 101489, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38925022

RESUMO

Dermatophytosis is a critical sort of skin infection caused by dermatophytes. The long-term treatment of such skin infections may be improved through the application of nanotechnology. This study aimed to prepare griseofulvin zinc Nanohybrid emulsion (GF-Zn-NHE) to improve griseofulvin activity against dermatophytes and some opportunistic pathogenic yeasts and bacteria. The GF-Zn-NHE is prepared by ultra-homogenization ultra-sonication strategies and validated by UV-visible spectroscopy analysis that confirms presences of griseofulvin and Zn-NPs peaks at 265 and 360 nm, respectively. The GF-Zn-NHE has mean distribution size 50 nm and zeta potential in the range from -40 to -36 mV with no significant changes in size distribution and particle size within 120 day ageing. Fourier transform infrared spectroscopy spectrum confirmed the presence of griseofulvin and Zn-NPs stretching vibration peaks. Gamma ray has a negative influence on GF-Zn-NE production and stability. GF-Zn-NHE drug release 95% up to 24 h and 98% up to 72 h of GF was observed and Zinc 90% up to 24 h and 95% up to 72 h, respectively. High antimicrobial activity was observed with GF-Zn-NHE against dermatophytic pathogens in compare with GF, GF-NE, zinc nitrate and ketoconazole with inhibition zone ranged from 14 to 36 mm. The results have shown that the MIC value for Cryptococcus neoformans, Prophyromonas gingivalis and Pseudomonas aeruginosa is 0.125 mg ml -1 and for Trichophyton rubrum, L. bulgaricus and Escherichia coli value is 0.25 mg ml -1 and for Candida albicans, Malassezia furfur and Enterococcus faecalis is 0.5 mg ml -1 and finally 1 mg ml -1 for Streptococcus mutans. TEM of treated Cryptococcus neoformans cells with GF-Zn-NHE displayed essentially modified morphology, degradation, damage of organelles, vacuoles and other structures.


Assuntos
Antifúngicos , Arthrodermataceae , Emulsões , Griseofulvina , Testes de Sensibilidade Microbiana , Zinco , Griseofulvina/farmacologia , Griseofulvina/química , Zinco/farmacologia , Zinco/química , Emulsões/farmacologia , Emulsões/química , Antifúngicos/farmacologia , Antifúngicos/química , Humanos , Arthrodermataceae/efeitos dos fármacos , Tamanho da Partícula , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Tinha/microbiologia , Tinha/tratamento farmacológico , Espectroscopia de Infravermelho com Transformada de Fourier , Nanopartículas Metálicas/química
19.
Mycopathologia ; 189(4): 52, 2024 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-38864945

RESUMO

Treatment-resistant dermatophytosis caused by the members of the Trichophyton mentagrophytes/Trichophyton interdigitale species group (TMTISG) is increasing worldwide. We aimed to determine the prevalence of TMTISG in patients with dermatophytosis in two centers from north of Iran and detect the possible mutations in the squalene epoxidase (SQLE) gene in relevant terbinafine (TRB) resistant pathogenic isolates. From November 2021 to December 2022, 1960 patients suspected to dermatophytosis and referred to two mycology referral laboratories in the north of Iran were included in the study. Identification of all dermatophyte isolates was confirmed by RFLP of rDNA internal transcribed spacer (ITS) regions. Antifungal susceptibility testing against five common antifungals using the CLSI-M38-A3 protocol was performed. The TMTISG isolates resistant to TRB, were further analyzed to determine the possible mutations in the SQLE gene. Totally, 647 cases (33%) were positive for dermatophytosis of which 280 cases (43.3%) were identified as members of TMTISG. These were more frequently isolated from tinea corporis 131 (44.56%) and tinea cruris 116 (39.46%). Of 280 TMTISG isolates, 40 (14.3%) were resistant to TRB (MIC ≥ 4 µg/mL), all found to be T. indotineae in ITS sequencing. In SQLE sequencing 34 (85%) of TRB-resistant isolates had coincident mutations of Phe397Leu and Ala448Thr whereas four and two isolates had single mutations of Phe397Leu and Leu393Ser, respectively. Overall, the resistance of Iranian TMTISG isolates to TRB greatly occurred by a mutation of Phe397Leu in the SQLE gene as alone or in combination with Ala448Thr. Nevertheless, for the occurrence of in vitro resistance, only the presence of Phe397Leu mutation seems to be decisive.


Assuntos
Antifúngicos , Arthrodermataceae , Farmacorresistência Fúngica , Testes de Sensibilidade Microbiana , Esqualeno Mono-Oxigenase , Terbinafina , Tinha , Irã (Geográfico)/epidemiologia , Farmacorresistência Fúngica/genética , Humanos , Antifúngicos/farmacologia , Terbinafina/farmacologia , Estudos Transversais , Tinha/microbiologia , Tinha/epidemiologia , Prevalência , Arthrodermataceae/genética , Arthrodermataceae/efeitos dos fármacos , Masculino , Feminino , Esqualeno Mono-Oxigenase/genética , Adulto , Pessoa de Meia-Idade , Mutação , Idoso , Adulto Jovem , Adolescente , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Criança
20.
Mycopathologia ; 189(4): 51, 2024 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-38864977

RESUMO

Data on the epidemiology of tinea capitis (TC), an infection of the scalp by dermatophytes, are scarce in Cameroon. This study aimed to determine the prevalence of TC among school-children in the Dschang Subdivision, Western Cameroon. A cross-sectional study was carried out in June 2021 in Dschang including pupils aged 5-13. First, a standardized questionnaire was administered to participant for the collection of sociodemographic data. Then, samples were collected and cultured onto Sabouraud-Chloramphenicol-Gentamicin Agar. The etiological agents were identified based on their morphological features and with MALDI-TOF mass spectrometry. A total of 1070 children were clinically examined and 108 (10.1%) children presented with TC lesions. The mean age of the 1070 participants was 8.3 ± 2.6 years (range: 5-13 years); 772 (72.2%) were males. The use of borehole water (OR = 0.01, 95%CI[0.001-0.03]), spring water (OR = 0.2, 95%CI[0.08-0.50]), rainwater (OR = 0.004, 95%CI[0.001-0.016]), and hairdressing salons visits (OR = 0.413, 95%CI[0.196-0.872]) were associated with a decreased TC risk in the multivariate logistic regression analysis. In contrast, sharing bed with siblings (OR = 4.48, 95%CI[2.095-9.60]) was associated with an increased TC risk in children. Among the 32 dermatophytes isolated in culture, Microsporum audouinii was the most frequent (43.8%), followed by Trichophyton rubrum (25.0%) and T. soudanense (25.0%). Microsporum canis and T. violaceum were both rarely isolated. Further studies are warranted to assess the association of TC with domestic water usage that has been highlighted in this study.


Assuntos
Tinha do Couro Cabeludo , Tinha do Couro Cabeludo/epidemiologia , Tinha do Couro Cabeludo/microbiologia , Humanos , Camarões/epidemiologia , Criança , Masculino , Feminino , Adolescente , Estudos Transversais , Pré-Escolar , Prevalência , Inquéritos e Questionários , Microsporum/isolamento & purificação , Fatores de Risco , Arthrodermataceae/isolamento & purificação , Arthrodermataceae/classificação , Instituições Acadêmicas , Trichophyton/isolamento & purificação
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