Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.704
Filtrar
1.
Pestic Biochem Physiol ; 204: 106033, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39277359

RESUMO

Spodoptera frugiperda has emerged as a major invasive pest worldwide. The utilization of chemical pesticides not only poses numerous ecological concerns but also fosters resistance in S. frugiperda. In this study, we designed and synthesized three novel thiothiazolidinone compounds (6a, 7b, and 7e) and incorporated innovative thiothiazolidinone structural elements into the piperine skeleton. Treatment with compounds 6a and 7e resulted in the blackening and agglomeration of oviduct eggs within the ovaries of certain female moths, impeding the release of normal eggs. The levels of vitellogenin and vitellogenin receptor, along with three trehalase inhibitors, exhibited a dynamic equilibrium state, leading to no discernible change in egg production but a notable increase in the generation of low-hatching-rate egg fragments. Compared with the injection of 2%DMSO, the eclosion rate of 6a injection was significantly decreased, as followed the spawning time and longevity were prolonged or significantly prolonged in the trehalase inhibitors of 6a, 7b, and 7e. We aimed to investigate the regulatory impacts of three new pepper thiothiazolidinone compounds on the reproduction of S. frugiperda, and to authenticate the efficacy of novel alginase inhibitors in inhibiting the reproduction of S. frugiperda. This research endeavors to aid in the identification of efficient and steadfast trehalase inhibitors, thereby expediting the research and development of potent biological pesticides.


Assuntos
Fertilidade , Spodoptera , Animais , Spodoptera/efeitos dos fármacos , Spodoptera/fisiologia , Feminino , Fertilidade/efeitos dos fármacos , Inseticidas/farmacologia , Capsicum , Trealase/metabolismo , Trealase/antagonistas & inibidores , Vitelogeninas/metabolismo , Tiazolidinas/farmacologia
2.
Pestic Biochem Physiol ; 204: 106058, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39277374

RESUMO

The juvenile hormone binding protein (JHBP) and takeout (TO) genes, mediated by the juvenile hormone (JH), play a crucial role in regulating the reproductive physiology of insects. Our previous study revealed that spinosad-resistant Frankliniella occidentalis (NIL-R) exhibited reduced fecundity and significant changes in JHBP/TO family gene expression. We hypothesized that these genes were involved in regulating the fitness costs associated with resistance. In this study, 45 JHBP/TO genes were identified in F. occidentalis, among which FoTO2 and FoTO10 were duplicates. Additionally, eight genes exhibited significant down-regulation in the NIL-R population. Two genes (FoTO6 and FoTO24) that exhibited the most significant differential expression between the spinosad-susceptible (Ivf03) and NIL-R populations were selected to investigate their roles in resistance fitness using RNA interference (RNAi). Following interference with FoTO6, FoTO24, and their combination, the expression levels of vitellogenin (Vg) were downregulated by 3%-30%, 13%-28%, and 14%-32% from the 2nd day to the 5th day, respectively; Krüppel-homolog 1 (Kr-h1) expression was down-regulated by 3%-65%, 11%-34%, and 11%-39% from the 2nd day to the 5th day, respectively; ovariole length was shortened by approximately 18%, 21%, and 24%, respectively; and the average number of eggs decreased from 407 to 260, 148, and 106, respectively. Additionally, a JH supplementation experiment on the NIL-R population revealed that the expression levels of both FoTO6, FoTO24, Vg and Kr-h1 were significantly upregulated compared with those observed in the Ivf03 population, resulting in increased fecundity. These results suggest that FoTO6 and FoTO24 are involved in JH-mediated regulation of the reproductive fitness cost of resistance to spinosad. Further, FoTO6 and FoTO24 can be considered potential target genes for applying RNAi technology in the scientific management of F. occidentalis.


Assuntos
Proteínas de Insetos , Resistência a Inseticidas , Tisanópteros , Animais , Resistência a Inseticidas/genética , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Tisanópteros/genética , Tisanópteros/fisiologia , Tisanópteros/efeitos dos fármacos , Inseticidas/farmacologia , Feminino , Reprodução/genética , Macrolídeos/farmacologia , Vitelogeninas/genética , Vitelogeninas/metabolismo , Combinação de Medicamentos , Hormônios Juvenis/metabolismo , Hormônios Juvenis/farmacologia , Interferência de RNA , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Aptidão Genética
3.
Development ; 151(20)2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-39171380

RESUMO

Delivering molecular tools into oocytes is essential for developmental and reproductive biology. Microinjection, the conventional method, is equipment intensive, often technically challenging and has a low yield, and is impractical in species with delicate oocytes or restricted spawning seasons. To overcome these limitations, we developed VitelloTag, a cost-effective, high-throughput system using vitellogenin-derived fusion proteins to enable efficient cargo delivery via receptor-mediated endocytosis. We demonstrate its utility by delivering Cas9/sgRNA complexes in two distantly related species for gene knockout.


Assuntos
Oócitos , Vitelogeninas , Oócitos/metabolismo , Animais , Vitelogeninas/metabolismo , Vitelogeninas/genética , Feminino , Sistemas CRISPR-Cas/genética , Endocitose , Técnicas de Inativação de Genes , Microinjeções/métodos , Peixe-Zebra/embriologia
4.
Cell Rep ; 43(8): 114571, 2024 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-39093698

RESUMO

Rice stripe virus (RSV) establishes infection in the ovaries of its vector insect, Laodelphax striatellus. We demonstrate that RSV infection delays ovarian maturation by inhibiting membrane localization of the vitellogenin receptor (VgR), thereby reducing the vitellogenin (Vg) accumulation essential for egg development. We identify the host protein L. striatellus Rab1 protein (LsRab1), which directly interacts with RSV nucleocapsid protein (NP) within nurse cells. LsRab1 is required for VgR surface localization and ovarian Vg accumulation. RSV inhibits LsRab1 function through two mechanisms: NP binding LsRab1 prevents GTP binding, and NP binding LsRab1-GTP complexes stimulates GTP hydrolysis, forming an inactive LsRab1 form. Through this dual inhibition, RSV infection prevents LsRab1 from facilitating VgR trafficking to the cell membrane, leading to inefficient Vg uptake. The Vg-VgR pathway is present in most oviparous animals, and the mechanisms detailed here provide insights into the vertical transmission of other insect-transmitted viruses of medical and agricultural importance.


Assuntos
Receptores de Superfície Celular , Tenuivirus , Proteínas rab1 de Ligação ao GTP , Animais , Feminino , Proteínas rab1 de Ligação ao GTP/metabolismo , Tenuivirus/fisiologia , Tenuivirus/metabolismo , Receptores de Superfície Celular/metabolismo , Proteínas do Ovo/metabolismo , Proteínas de Insetos/metabolismo , Proteínas de Insetos/genética , Vitelogeninas/metabolismo , Proteínas do Nucleocapsídeo/metabolismo , Hemípteros/virologia , Hemípteros/metabolismo , Ovário/virologia , Ovário/metabolismo , Ligação Proteica , Transporte Proteico , Membrana Celular/metabolismo , Membrana Celular/virologia , Doenças das Plantas/virologia
5.
Aquat Toxicol ; 274: 107052, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39163697

RESUMO

To investigate whether environmental concentrations of fragmented polyethylene terephthalate (PET) microplastics (MPs) have additional or combined effects on endocrine-disrupting activity, Java medaka (Oryzias javanicus) were exposed to 17ß-estradiol (E2; 5, 10, 50, and 100 ng L-1), bisphenol A (BPA; 5, 10, 50, and 100 µg L-1), and E2 and BPA combined with PET MPs (1 and 100 particles L-1) for 200 days. The growth parameters, such as body length and weight, were significantly decreased by the highest concentrations of E2 and BPA. A significant reduction in egg production was observed in female fish exposed to BPA, with an additive toxic effect of PET MPs. A female-biased sex ratio was observed in fish exposed to both chemicals. Exposure to E2 significantly increased the hepatosomatic index (HSI) in both sexes, while no significant effect was observed in the gonadosomatic index (GSI). Exposure to BPA significantly increased the HSI in female fish and decreased the GSI in both sexes of fish. An additive effect of PET MPs was observed on the GSI value of female exposed to BPA. Significant elevations in vitellogenin (VTG) levels were observed in both sexes due to exposure to E2 and BPA. Additive effects of PET MPs were observed on VTG levels in males exposed to E2 and BPA. Taken together, even long-term treatment with PET MPs induced only a negligible additive effect on the endocrine-disrupting activity in Java medaka at environmentally relevant concentrations.


Assuntos
Compostos Benzidrílicos , Estradiol , Microplásticos , Oryzias , Fenóis , Polietilenotereftalatos , Reprodução , Poluentes Químicos da Água , Animais , Oryzias/fisiologia , Oryzias/crescimento & desenvolvimento , Compostos Benzidrílicos/toxicidade , Fenóis/toxicidade , Microplásticos/toxicidade , Poluentes Químicos da Água/toxicidade , Feminino , Masculino , Reprodução/efeitos dos fármacos , Polietilenotereftalatos/toxicidade , Disruptores Endócrinos/toxicidade , Vitelogeninas/metabolismo
6.
Environ Sci Pollut Res Int ; 31(39): 51954-51970, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39136918

RESUMO

Phthalates, such as di-n-butyl phthalate (DBP) and di-isopentyl phthalate (DiPeP), are pollutants with a high potential for endocrine disruption. This study aimed to evaluate parameters of endocrine disruption in specimens of the Neotropical fish Rhamdia quelen exposed to DBP and DiPeP through their food. After 30 days of exposure, the fish were anesthetized and then euthanized, and blood, hypothalamus, liver, and gonads were collected. DBP caused statistically significant alterations in the serotoninergic system of males (5 and 25 ng/g) and females (5 ng/g) of R. quelen and it increased testosterone levels in females (25 ng/g). DiPeP significantly altered the dopaminergic system in females, reduced plasma estradiol levels (125 ng/g) and hepatic vitellogenin expression (25 ng/g), and changed the antioxidant system in gonads (125 ng/g). The results suggest that DBP and DiPeP may have different response patterns in females, with the former being androgenic and the latter being anti-estrogenic. These findings provide additional evidence regarding the molecular events involving DBP and DiPeP in the endocrine disruption potential in juvenile specimens of Rhamdia quelen.


Assuntos
Antioxidantes , Peixes-Gato , Dibutilftalato , Disruptores Endócrinos , Neurotransmissores , Vitelogeninas , Animais , Vitelogeninas/metabolismo , Vitelogeninas/sangue , Dibutilftalato/toxicidade , Disruptores Endócrinos/toxicidade , Feminino , Antioxidantes/metabolismo , Masculino , Neurotransmissores/metabolismo , Poluentes Químicos da Água/toxicidade , Ácidos Ftálicos/toxicidade , Gônadas/efeitos dos fármacos
7.
Artigo em Inglês | MEDLINE | ID: mdl-39111515

RESUMO

The frequency of detection and concentrations of bifenthrin, a pyrethroid insecticide, in the waterways inhabited by the endangered species, steelhead trout (Oncorhynchus mykiss), has become a significant concern for regulatory agencies. Endocrine disruption has been observed with estrogenic and anti-estrogenic responses in fish species at different life stages. Since several studies have indicated alterations in dopaminergic signaling associated with endocrine responses, juvenile steelhead were exposed to environmentally relevant concentrations of 60 or 120 ng/L bifenthrin for two weeks. Fish brains were assessed for dopamine levels and the expression of genes involved in dopaminergic and estrogenic processes, such as catechol-o-methyltransferase (comt) and monoamine oxidase (mao). Vitellogenin (vtg) and estrogenic receptors (ERα1, ERß1, and ERß2) were also evaluated in livers of the animals. Dopamine concentrations were significantly higher in fish brains following bifenthrin exposure. Consistent with a reduction in dopamine clearance, there was a significant decrease in the mRNA expression of comt with increased bifenthrin concentration. Hepatic expression of ERα1 and ERß2 mRNA was significantly decreased with increased bifenthrin concentration. These data support the possible mechanism of bifenthrin altering the dopaminergic pathway at low ng/L concentrations, in juvenile steelhead, which could interfere with endocrine feedback loops. These findings support the need for and importance of identifying species and life stage differences in pesticide modes of action to reduce uncertainties in risk assessments.


Assuntos
Encéfalo , Dopamina , Inseticidas , Oncorhynchus mykiss , Piretrinas , Poluentes Químicos da Água , Animais , Piretrinas/toxicidade , Poluentes Químicos da Água/toxicidade , Oncorhynchus mykiss/metabolismo , Dopamina/metabolismo , Inseticidas/toxicidade , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Catecol O-Metiltransferase/genética , Catecol O-Metiltransferase/metabolismo , Disruptores Endócrinos/toxicidade , Receptores de Estrogênio/metabolismo , Receptores de Estrogênio/genética , Proteínas de Peixes/metabolismo , Proteínas de Peixes/genética , Vitelogeninas/metabolismo , Vitelogeninas/genética
8.
Arch Insect Biochem Physiol ; 116(3): e22125, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38973236

RESUMO

Insect pest control can be achieved by the application of RNA interference (RNAi), a key molecular tool in functional genomics. Whereas most RNAi research has focused on insect pests, few studies have been performed on natural enemies. Validating the efficacy of RNAi in natural enemies is crucial for assessing its safety and enabling molecular research on these organisms. Here, we assessed the efficacy of RNAi in the ladybird beetle Eriopis connexa Germar (Coleoptera: Coccinellidae), focusing on genes related to reproduction, such as vitellogenin (Vg) and its receptor (VgR). In the transcriptome of E. connexa, we found one VgR (EcVgR) and two Vg genes (EcVg1 and EcVg2). These genes have been validated by in silico analyses of functional domains and evolutionary relationships. Five-day-old females were injected with 500 ng/µL of a specific double-stranded RNA (dsRNA) (dsEcVg1, dsEcVg2, or dsEcVgR) for RNAi tests, while nonspecific dsRNA (dsGFP or dsAgCE8.1) was used as a control. Interestingly, dsEcVg2 was able to knockdown both Vg genes, while dsEcVg1 could silence only EcVg1. Additionally, the viability of the eggs was significantly reduced when both Vg genes were knocked down at the same time (after treatment with dsEcVg2 or "dsEcVg1+dsEcVg2"). Ultimately, malformed, nonviable eggs were produced when EcVgR was silenced. Interestingly, no dsRNA treatment had an impact on the quantity of eggs laid. Therefore, the feasibility of RNAi in E. connexa has been confirmed, suggesting that this coccinellid is an excellent Neotropical model for molecular research on natural enemies and for studying RNAi nontarget effects.


Assuntos
Besouros , Técnicas de Silenciamento de Genes , Interferência de RNA , Animais , Besouros/genética , Feminino , Vitelogeninas/genética , Vitelogeninas/metabolismo , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Reprodução/genética , RNA de Cadeia Dupla/genética , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Proteínas do Ovo/genética , Proteínas do Ovo/metabolismo , Controle Biológico de Vetores
9.
Environ Pollut ; 359: 124525, 2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-39004206

RESUMO

With aging population increasing globally, the use of pharmaceutically active compounds is rising. The cardiovascular drug telmisartan has been widely detected in various environmental compartments, including biota, surface waters, and sewage treatment plant effluents at concentrations ranging from ng/L to µg/L levels. This study evaluated the effects of telmisartan on the microcrustacean Daphnia magna at a wide range of concentrations (0.35, 0.70, 1.40, 500, and 1000 µg/L) and revealed significant ecotoxicological implications of this drug, even at environmentally relevant concentration. Acute exposure to telmisartan (1.40, 500, and 1000 µg/L) resulted in a notable decrease in heart rate, while chronic exposure accelerated the time to the first brood by 3 days and reduced neonate body size. Molecular investigations revealed marked downregulation of vitellogenin genes (Vtg1 and Vtg2). Non-monotonic dose responses were observed for gene expression, early-stage body length, and the total number of offspring produced, while the heart rate and time to the first brood showed clear concentration-dependent responses. These findings highlight the potential risks, notably to reproductive capacity, associated with exposure to telmisartan in environmentally relevant concentration, suggesting the need for further studies on the potential long-term ecological consequences.


Assuntos
Daphnia , Reprodução , Telmisartan , Poluentes Químicos da Água , Animais , Daphnia/efeitos dos fármacos , Daphnia/genética , Telmisartan/toxicidade , Poluentes Químicos da Água/toxicidade , Reprodução/efeitos dos fármacos , Vitelogeninas/genética , Vitelogeninas/metabolismo , Daphnia magna
10.
Pestic Biochem Physiol ; 203: 106014, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39084805

RESUMO

Energy metabolism is essential for insect development, reproduction and detoxification. Insects often reallocate energy and resources to manage external stress, balancing the demands of detoxification and reproduction. Glucose transport 4 (Glut4), a glucose transporter, is involved in glucose and lipid metabolism. However, the specific molecular mechanism of Glut4 in insect reproduction, and its role in the response to insecticide-induced oxidative stress remain unclear. In this study, LmGlut4 was identified and analyzed in Locusta migratoria. Silencing of LmGlut4 significantly reduced vitellogenin (Vg) biosynthesis in the fat body and Vg absorption by oocytes, ultimately hindering ovarian development and oocyte maturation. Knockdown of LmGlut4 also inhibited the biosynthesis of key insect hormones, such as juvenile hormone (JH), 20-hydroxyecdysone (20E) and insulin. Furthermore, LmGlut4 knockdown led to reduced triglyceride (TG) and glycogen content in the fat body and ovary, as well as decreased capacity for trehalose biosynthesis in adipocytes. Additionally, dsLmGlut4-treated locusts showed heightened sensitivity to deltamethrin, leading to increased triglyceride depletion during detoxification. This study sheds light on the biological function of LmGlut4 in the ovary and provides potential target genes for exploring biological pest management strategies.


Assuntos
Transportador de Glucose Tipo 4 , Inseticidas , Locusta migratoria , Nitrilas , Ovário , Piretrinas , Interferência de RNA , Animais , Piretrinas/farmacologia , Feminino , Nitrilas/farmacologia , Ovário/metabolismo , Ovário/efeitos dos fármacos , Transportador de Glucose Tipo 4/metabolismo , Transportador de Glucose Tipo 4/genética , Locusta migratoria/genética , Locusta migratoria/efeitos dos fármacos , Locusta migratoria/metabolismo , Inseticidas/farmacologia , Proteínas de Insetos/metabolismo , Proteínas de Insetos/genética , Vitelogeninas/metabolismo , Vitelogeninas/genética , Metabolismo Energético/efeitos dos fármacos , Corpo Adiposo/metabolismo , Corpo Adiposo/efeitos dos fármacos , Hormônios Juvenis/metabolismo , Hormônios Juvenis/farmacologia , Oócitos/metabolismo , Oócitos/efeitos dos fármacos , Triglicerídeos/metabolismo
12.
Aquat Toxicol ; 273: 107023, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39059103

RESUMO

Nanoplastics (NPs) have been commonly detected in aquatic ecosystems, and their negative effects on aquatic organisms have raised concerns in the scientific community and general public. The acute toxicity, neurotoxicity, and metabolic toxicity induced by NPs on fishes have been reported by many studies, although less attention has been focused on how mother exposed to NPs affected their offspring in aquatic organisms. Here, female zebrafish (F0) were exposed to 0, 200 and 2000 µg/L polystyrene nanoplastics (PS-NPs) for 42 d, with their offspring (F1) reared in clear water until sexual maturity. The results showed that PS-NPs were detected in various organs of F0 and F1. PS-NPs exposure significantly decreased gonadal 17-estradiol (E2), while increasing testosterone (T) contents. Lower levels of cyp19a1a, lhr and erα expressions in the 2000 µg/L group were consistent with a reduced number of mature oocytes (MO), but an increase in perinucleolar oocytes (PO). Interestingly, the expression of vtg was only up-regulated by 200 µg/L PS-NPs. After exposure, the egg production was dramatically reduced, but the hatching rate and heartbeat of F1 embryos from treated females were significantly higher than those observed in females from the control group. Maternal PS-NPs exposure significantly decreased the E2 and T levels in F1 adults, while PS-NPs exposure significantly up-regulated the sox9a but down-regulated the foxl2a in F1 larvae of 30 days post fertilization (dpf). This study showed that PS-NPs caused reproductive toxicity by changing the hypothalamic-pituitary-gonadal (HPG) axis-related genes, impairing the reproductive capacity of female zebrafish, affecting the development and disrupting the endocrine function of F1. These results suggested that PS-NPs had adverse effects on fish reproductive system both in the directly exposed generation and in their unexposed offspring.


Assuntos
Poliestirenos , Reprodução , Poluentes Químicos da Água , Peixe-Zebra , Animais , Feminino , Poluentes Químicos da Água/toxicidade , Poliestirenos/toxicidade , Reprodução/efeitos dos fármacos , Estradiol , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismo , Testosterona/metabolismo , Nanopartículas/toxicidade , Embrião não Mamífero/efeitos dos fármacos , Exposição Materna , Vitelogeninas/metabolismo , Oócitos/efeitos dos fármacos
13.
Int J Mol Sci ; 25(12)2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38928442

RESUMO

To enhance our understanding of teleost reproductive physiology, we identified six Sichuan bream (Sinibrama taeniatus) vitellogenin genes (vtg1-6) and characterized their sequence structures. We categorized them into type Ⅰ (vtg1,4,5 and 6), type Ⅱ (vtg2) and type Ⅲ (vtg3) based on differences in their subdomain structure. The promoter sequence of vtgs has multiple estrogen response elements, and their abundance appears to correlate with the responsiveness of vtg gene expression to estrogen. Gene expression analyses revealed that the vitellogenesis of Sichuan bream involves both heterosynthesis and autosynthesis pathways, with the dominant pathway originating from the liver. The drug treatment experiments revealed that 17ß-estradiol (E2) tightly regulated the level of vtg mRNA in the liver. Feeding fish with a diet containing 100 µg/g E2 for three weeks significantly induced vtg gene expression and ovarian development, leading to an earlier onset of vitellogenesis. Additionally, it was observed that the initiation of vtg transcription required E2 binding to its receptor, a process primarily mediated by estrogen receptor alpha in Sichuan bream. The findings of this study provide novel insights into the molecular information of the vitellogenin gene family in teleosts, thereby contributing to the regulation of gonadal development in farmed fish.


Assuntos
Estrogênios , Vitelogeninas , Animais , Vitelogeninas/genética , Vitelogeninas/metabolismo , Estrogênios/metabolismo , Estrogênios/farmacologia , Vitelogênese/genética , Estradiol/farmacologia , Estradiol/metabolismo , Regiões Promotoras Genéticas , Feminino , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Filogenia , Regulação da Expressão Gênica/efeitos dos fármacos , Família Multigênica , Fígado/metabolismo , Genoma , Receptor alfa de Estrogênio/genética , Receptor alfa de Estrogênio/metabolismo
14.
Aquat Toxicol ; 273: 107000, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38875953

RESUMO

Nodularin is a potent cyanotoxin that has been detected in aquatic environments as well as in the body of aquatic organisms throughout the world, but its effects on the reproductive system are yet to be explored. The present study investigated the toxic effects of environmentally relevant concentrations of nodularin on the reproductive endocrine system of female zebrafish (Danio rerio). After exposure to nodularin for 14 days, decreased gonadosomatic Index (GSI), germinal vesicle breakdown (GVBD), and decreased level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17ß-estradiol (E2) level and increased testosterone (T) content in female zebrafish suggested that nodularin may disrupt both oocyte growth and maturation. In support of this data, alteration in different marker gene expression on the hypothalamic-pituitary-gonadal-liver (HPGL) axis was observed. Transcriptional levels of genes related to steroidogenesis including cytochrome P450 aromatase (cyp19a1a) in the ovary and primary vitellogenin genes (vtg1, vtg2, and vtg3) in the liver were down-regulated and marker genes for oxidative stress (sod, cat, and gpx) were up-regulated on HPGL axis. These findings revealed for the first time that nodularin is a potent endocrine-disrupting compound posing oxidative stress and causes reproductive endocrine toxicity in female zebrafish, emphasizing the importance of assessing its environmental risks.


Assuntos
Ovário , Poluentes Químicos da Água , Peixe-Zebra , Animais , Peixe-Zebra/fisiologia , Feminino , Poluentes Químicos da Água/toxicidade , Ovário/efeitos dos fármacos , Vitelogeninas/genética , Vitelogeninas/metabolismo , Sistema Endócrino/efeitos dos fármacos , Estradiol , Disruptores Endócrinos/toxicidade , Reprodução/efeitos dos fármacos , Fígado/efeitos dos fármacos , Testosterona , Estresse Oxidativo/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismo , Hormônio Foliculoestimulante , Hormônio Luteinizante
15.
Aquat Toxicol ; 273: 107004, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38901218

RESUMO

The extensive use of herbicide metamifop (MET) in rice fields for weeds control will inevitably lead to its entering into water environments and threaten the aquatic organisms. Previous researches have demonstrated that sublethal exposure of MET significantly affected zebrafish development. Yet the long-term toxicological impacts of MET on aquatic life remains unknown. Herein, we investigated the potential effects of MET (5 and 50 µg/L) on zebrafish during an entire life cycle. Since the expression level of male sex differentiation-related gene dmrt1 and sex hormone synthesis-related gene cyp19a1b were significantly changed after 50 µg/L MET exposure for only 7 days, indicators related to sex differentiation and reproductive system were further investigated. Results showed that the transcript of dmrt1 was inhibited, estradiol content increased and testosterone content decreased in zebrafish of both sexes after MET exposure at 45, 60 and 120 dpf. Histopathological sections showed that the proportions of mature germ cells in the gonads of male and female zebrafish (120 dpf) were significantly decreased. Moreover, males had elevated vitellogenin content while females did not after MET exposure; MET induced feminization in zebrafish, with the proportion of females significantly increased by 19.6% while that of males significantly decreased by 13.2% at 120 dpf. These results suggested that MET interfered with the expression levels of gonad development related-genes, disrupted sex hormone balance, and affected sex differentiation and reproductive system of female and male zebrafish, implying it might have potential endocrine disrupting effects after long-term exposure.


Assuntos
Diferenciação Sexual , Vitelogeninas , Poluentes Químicos da Água , Peixe-Zebra , Animais , Diferenciação Sexual/efeitos dos fármacos , Masculino , Feminino , Poluentes Químicos da Água/toxicidade , Vitelogeninas/metabolismo , Vitelogeninas/genética , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismo , Herbicidas/toxicidade , Aromatase/genética , Aromatase/metabolismo , Estradiol , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Testosterona , Gônadas/efeitos dos fármacos , Reprodução/efeitos dos fármacos
16.
Aquat Toxicol ; 273: 107002, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38936242

RESUMO

This study aimed to investigate the toxicity and endocrine disrupting potential of a complex mixture of polycyclic aromatic hydrocarbons (PAHs) in the estrogen pathway using hepatocytes of Nile tilapia Oreochromis niloticus, the hepatocytes were exposed to various concentrations of the PAH mixture, and multiple endpoints were evaluated to assess their effects on cell viability, gene expression, oxidative stress markers, and efflux activity. The results revealed that the PAH mixture had limited effects on hepatocyte metabolism and cell adhesion, as indicated by the non-significant changes observed in MTT metabolism, neutral red retention, and crystal violet staining. However, significant alterations were observed in the expression of genes related to the estrogen pathway. Specifically, vitellogenin (vtg) exhibited a substantial increase of approximately 120% compared to the control group. Similarly, estrogen receptor 2 (esr2) showed a significant upregulation of approximately 90%. In contrast, no significant differences were observed in the expression of estrogen receptor 1 (esr1) and the G protein-coupled estrogen receptor 1 (gper1). Furthermore, the PAH mixture elicited complex responses in oxidative stress markers. While reactive oxygen species (ROS) and reactive nitrogen species (RNS) levels remained unchanged, the activity of catalase (Cat) was significantly reduced, whereas superoxide dismutase (Sod) activity, glutathione S-transferase (Gst) activity, and non-protein thiols levels were significantly elevated. In addition, the PAH mixture significantly influenced efflux activity, as evidenced by the increased efflux of rhodamine and calcein, indicating alterations in multixenobiotic resistance (MXR)-associated proteins. Overall, these findings, associated with bioinformatic analysis, highlight the potential of the PAH mixture to modulate the estrogen pathway and induce oxidative stress in O. niloticus hepatocytes. Understanding the mechanisms underlying these effects is crucial for assessing the ecological risks of PAH exposure and developing appropriate strategies to mitigate their adverse impacts on aquatic organisms.


Assuntos
Ciclídeos , Disruptores Endócrinos , Estrogênios , Hepatócitos , Estresse Oxidativo , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Animais , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Ciclídeos/metabolismo , Ciclídeos/genética , Poluentes Químicos da Água/toxicidade , Disruptores Endócrinos/toxicidade , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Estrogênios/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Vitelogeninas/metabolismo , Vitelogeninas/genética , Espécies Reativas de Oxigênio/metabolismo
17.
Chemosphere ; 362: 142616, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38906194

RESUMO

The water accommodated fraction (WAF) of spilled crude oil is a severe threat to the health of marine fish. This study was conducted to investigate the effects of short-term embryonic exposure to the WAF on the ovarian development and reproductive capability of F0 adult female marine medaka (Oryzias melastigma). Following embryonic exposure to the WAF with nominal total petroleum hydrocarbon concentrations of 0.5, 5, 50, and 500 µg/L for 7 days, the number of spawned eggs and gonadosomatic indices of F0 adult females were significantly reduced at 130 days postfertilization. In these F0 adult females, the proportion of mature oocytes was significantly lower, the level of 17ß-estradiol was lower, and the level of testosterone was greater than those in control group. The mRNA levels of the follicle-stimulating hormone ß subunit, luteinizing hormone ß subunit, cytochrome P450 aromatase 19b, estrogen receptor α and ß, and androgen receptor α and ß genes were upregulated, while the mRNA level of the salmon-type gonadotropin-releasing hormone was downregulated in F0 adult females exposed to the WAF during the embryonic stage. Additionally, the methylation level of vitellogenin (vtg) in F0 adult females was significantly elevated, this might have, in turn, downregulated the mRNA level of vtg. The mortality rate of the unexposed F1 embryos was significantly increased and the hatching success was significantly reduced. These results collectively indicated the necessity of incorporating and evaluating the effects of short-term early-life exposure to crude oil in the assessment of risks to the reproductive health of marine fish.


Assuntos
Oryzias , Petróleo , Reprodução , Vitelogeninas , Poluentes Químicos da Água , Animais , Feminino , Oryzias/fisiologia , Poluentes Químicos da Água/toxicidade , Petróleo/toxicidade , Reprodução/efeitos dos fármacos , Vitelogeninas/metabolismo , Vitelogeninas/genética , Estradiol , Embrião não Mamífero/efeitos dos fármacos , Poluição por Petróleo , Aromatase/metabolismo , Aromatase/genética , Ovário/efeitos dos fármacos , Testosterona/metabolismo
18.
Vet Parasitol ; 329: 110212, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38781831

RESUMO

Tick infestations transmit various infectious agents and result in significant socioeconomic consequences. Currently, the primary focus of tick control efforts is identifying potential targets for immune intervention. In a previous study, we identified a highly conserved protein abundant in tick haemolymph extracellular vesicles (EVs) known as translationally controlled tumour protein (TCTP). We have found that native TCTP is present in various tissues of the Rhipicephalus haemaphysaloides tick, including salivary glands, midgut, ovary, and fat body. Notably, TCTP is particularly abundant in the tick ovary and its levels increase progressively from the blood-feeding stage to engorgement. When the TCTP gene was knocked down by RNAi, there was a noticeable delay in ovarian development, and the reproductive performance, in terms of egg quantity and survival, was also hindered. Our investigations have revealed that the observed effects in ovary and eggs in dsRNA-treated ticks are not attributable to cell death mechanisms like apoptosis and autophagy but rather to the reduction in the expression of vitellogenin (Vg1, Vg2, and Vg3) and ferritin (ferritin 1 and ferritin 2) proteins crucial for ovarian development and embryo survival in ticks. Additionally, phylogenetic analysis and structural comparisons of RhTCTP and its orthologues across various tick species, vertebrate hosts, and humans have shown that TCTP is conserved in ticks but differs significantly between ticks and their hosts, particularly in the TCTP_1 and TCTP_2 domains. Overall, TCTP plays a vital role in tick reproductive development and presents itself as a potential target for tick control in both humans and animals.


Assuntos
Ovário , Oviposição , Rhipicephalus , Proteína Tumoral 1 Controlada por Tradução , Animais , Feminino , Rhipicephalus/genética , Rhipicephalus/fisiologia , Rhipicephalus/crescimento & desenvolvimento , Filogenia , Vitelogeninas/genética , Vitelogeninas/metabolismo , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo
19.
Arch Insect Biochem Physiol ; 116(1): e22120, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38739744

RESUMO

The vitellogenin receptor (VgR) is essential for the uptake and transport of the yolk precursor, vitellogenin (Vg). Vg is synthesized in the fat body, released in the hemolymph, and absorbed in the ovaries, via receptor-mediated endocytosis. Besides its important role in the reproductive pathway, Vg occurs in nonreproductive worker honey bee, suggesting its participation in other pathways. The objective was to verify if the VgR occurs in the hypopharyngeal glands of Apis mellifera workers and how Vg is internalized by these cells. VgR occurrence in the hypopharyngeal glands was evaluated by qPCR analyses of VgR and immunohistochemistry in workers with different tasks. The VgR gene is expressed in the hypopharyngeal glands of workers with higher transcript levels in nurse honey bees. VgR is more expressed in 11-day-old workers from queenright colonies, compared to orphan ones. Nurse workers with developed hypopharyngeal glands present higher VgR transcripts than those with poorly developed glands. The immunohistochemistry results showed the co-localization of Vg, VgR and clathrin (protein that plays a major role in the formation of coated vesicles in endocytosis) in the hypopharyngeal glands, suggesting receptor-mediated endocytosis. The results demonstrate that VgR performs the transport of Vg to the hypopharyngeal glands, supporting the Ovary Ground Plan Hypothesis and contributing to the understanding of the role of this gland in the social context of honey bees.


Assuntos
Proteínas do Ovo , Hipofaringe , Proteínas de Insetos , Receptores de Superfície Celular , Animais , Abelhas/metabolismo , Abelhas/genética , Receptores de Superfície Celular/metabolismo , Receptores de Superfície Celular/genética , Proteínas de Insetos/metabolismo , Proteínas de Insetos/genética , Proteínas do Ovo/metabolismo , Proteínas do Ovo/genética , Hipofaringe/metabolismo , Feminino , Vitelogeninas/metabolismo , Vitelogeninas/genética , Clatrina/metabolismo
20.
Insect Biochem Mol Biol ; 170: 104139, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38815735

RESUMO

Peroxisomes are ubiquitous cellular organelles participating in a variety of critical metabolic reactions. PEX14 is an essential peroxin responsible for peroxisome biogenesis. In this study, we identified the human PEX14 homolog in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). N. lugens PEX14 (NlPEX14) showed significant topological similarity to its human counterpart. It is expressed throughout all developmental stages, with the highest expression observed in adult insects. Down-regulation of NlPEX14 through injection of NlPEX14-specific double-strand RNA impaired nymphal development. Moreover, females subjected to dsNlPEX14 treatment exhibited a significantly reduced lifespan. Additionally, we found abnormal ovarian development and a significant decrease in the number of eggs laid in NlPEX14-downregulated females. Further experiments support that the shortening of lifespan and the decrease in female fecundity can be attributed, at least partially, to the accumulation of fatty acids and reduced expression of vitellogenin. Together, our study reveals an indispensable function of NlPEX14 for insect reproduction and establishes a causal connection between the phenotypes and peroxisome biogenesis, shedding light on the importance of peroxisomes in female fecundity.


Assuntos
Fertilidade , Hemípteros , Proteínas de Insetos , Animais , Hemípteros/genética , Hemípteros/metabolismo , Hemípteros/fisiologia , Hemípteros/crescimento & desenvolvimento , Feminino , Proteínas de Insetos/metabolismo , Proteínas de Insetos/genética , Peroxissomos/metabolismo , Longevidade , Ninfa/crescimento & desenvolvimento , Ninfa/metabolismo , Ninfa/genética , Peroxinas/metabolismo , Peroxinas/genética , Proteínas de Membrana/metabolismo , Proteínas de Membrana/genética , Vitelogeninas/metabolismo , Vitelogeninas/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA