RESUMEN
Using the self as a reference point at encoding produces a memory advantage over other types of encoding activities. Even simply co-presenting a target item with self-relevant versus other-relevant information can produce an "incidental" self-memory advantage in the absence of any explicit task demand to evaluate the item's self-relevancy. In the present study, we asked whether an incidental self-memory advantage results from (a) the mere co-presentation of a target item with self-relevant information at encoding or (b) relational processing between a target item and self-relevant information at encoding. During incidental encoding, words were presented in two different colors either above or below a name (the participant's own or another person's). Participants judged either the location of each word in relation to the name ("Is the word above or below the name?") or the color of each word to which the name had no relevance ("Is the word in red or green?"). In a subsequent memory test, we found a self-memory advantage for both items and their associated source features in the location judgment task but not in the color judgment task. Our findings show that a memory advantage for a target item presented with self-relevant versus other-relevant information is more likely when a task agenda places, via relational processing demands, the self-relevant/other-relevant information in the focus of attention along with the target item. Potential processes that mediate this attention-dependent effect are discussed.
Asunto(s)
Atención , Memoria , Autoimagen , Procesamiento Espacial , Adulto , Femenino , Humanos , Masculino , Adulto JovenRESUMEN
High abundance proteins like protease inhibitors of plasma display a multitude of interactions in natural environments. Quantitative analysis of such interactions in vivo is essential to study diseases, but have not been forthcoming, as most methods cannot be directly applied in a complex biological environment. Here, we report a quantitative microscale thermophoresis assay capable of deciphering functional deviations from in vitro inhibition data by combining concentration and affinity measurements. We obtained stable measurement signals for the substrate-like interaction of the disease relevant inhibitor α-1-antitrypsin (AAT) Z-variant with catalytically inactive elastase. The signal differentiates between healthy and sick AAT-deficient individuals suggesting that affinity between AAT and elastase is strongly modulated by so-far overlooked additional binding partners from the plasma.
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Análisis Químico de la Sangre/métodos , Elastasa de Leucocito/sangre , alfa 1-Antitripsina/sangre , Análisis Químico de la Sangre/normas , Dominio Catalítico , Células HEK293 , Humanos , Elastasa de Leucocito/química , Elastasa de Leucocito/metabolismo , Unión Proteica , Sensibilidad y Especificidad , alfa 1-Antitripsina/química , alfa 1-Antitripsina/metabolismoRESUMEN
An imbalance between neutrophil-derived proteases and extracellular inhibitors is widely regarded as an important pathogenic mechanism for lung injury. Despite intense efforts over the last three decades, attempts to develop small-molecule inhibitors for neutrophil elastase have failed in the clinic. Here we discover an intrinsic self-cleaving property of mouse neutrophil elastase that interferes with the action of elastase inhibitors. We show that conversion of the single-chain (sc) into a two-chain (tc) neutrophil elastase by self-cleavage near its S1 pocket altered substrate activity and impaired both inhibition by endogenous α-1-antitrypsin and synthetic small molecules. Our data indicate that autoconversion of neutrophil elastase decreases the inhibitory efficacy of natural α-1-antitrypsin and small-molecule inhibitors, while retaining its pathological potential in an experimental mouse model. The so-far overlooked occurrence and properties of a naturally occurring tc-form of neutrophil elastase necessitates the redesign of small-molecule inhibitors that target the sc-form as well as the tc-form of neutrophil elastase.
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Elastasa de Leucocito/metabolismo , Pulmón/efectos de los fármacos , Neutrófilos/efectos de los fármacos , Péptidos/metabolismo , Proteínas Inhibidoras de Proteinasas Secretoras/farmacología , Enfisema Pulmonar/tratamiento farmacológico , Secuencia de Aminoácidos , Animales , Dominio Catalítico , Citocinas/biosíntesis , Femenino , Expresión Génica , Células HEK293 , Humanos , Elastasa de Leucocito/administración & dosificación , Elastasa de Leucocito/antagonistas & inhibidores , Elastasa de Leucocito/química , Pulmón/enzimología , Pulmón/patología , Macrófagos/efectos de los fármacos , Macrófagos/patología , Ratones , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , Neutrófilos/enzimología , Neutrófilos/patología , Péptidos/administración & dosificación , Péptidos/antagonistas & inhibidores , Péptidos/química , Proteolisis , Enfisema Pulmonar/inducido químicamente , Enfisema Pulmonar/enzimología , Enfisema Pulmonar/patología , Proteínas Recombinantes/administración & dosificación , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Homología de Secuencia de Aminoácido , Bibliotecas de Moléculas Pequeñas/farmacología , alfa 1-Antitripsina/farmacologíaRESUMEN
Glucose is the molecule that drives milk production, and insulin plays a pivotal role in the glucose metabolism of dairy cows. The effect of insulin on the glucose metabolism is regulated by the secretion of insulin by the pancreas and the insulin sensitivity of the skeletal muscles, the adipose tissue, and the liver. Insulin resistance may develop as part of physiologic (pregnancy and lactation) and pathologic processes, which may manifest as decreased insulin sensitivity or decreased insulin responsiveness. A good knowledge of the normal physiology of insulin is needed to measure the in vivo insulin resistance of dairy cows.
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Enfermedades de los Bovinos/metabolismo , Glucosa/metabolismo , Resistencia a la Insulina , Insulina/metabolismo , Lactancia/metabolismo , Tejido Adiposo/metabolismo , Animales , Bovinos , Enfermedades de los Bovinos/fisiopatología , Femenino , Secreción de Insulina , Lactancia/fisiología , Hígado/metabolismo , Músculo Esquelético/metabolismo , EmbarazoRESUMEN
OBJECTIVE: To study the longitudinal dynamics of anti-myelin oligodendrocyte glycoprotein (MOG) autoantibodies in childhood demyelinating diseases. METHODS: We addressed the kinetics of anti-MOG immunoglobulins in a prospective study comprising 77 pediatric patients. This was supplemented by a cross-sectional study analyzing 126 pediatric patients with acute demyelination and 62 adult patients with multiple sclerosis (MS). MOG-transfected cells were used for detection of antibodies by flow cytometry. RESULTS: Twenty-five children who were anti-MOG immunoglobulin (Ig) positive at disease onset were followed for up to 5 years. Anti-MOG antibodies rapidly and continuously declined in all 16 monophasic patients with acute disseminated encephalomyelitis and in one patient with clinically isolated syndrome. In contrast, in 6 of 8 patients (75%) eventually diagnosed with childhood MS, the antibodies to MOG persisted with fluctuations showing a second increase during an observation period of up to 5 years. Antibodies to MOG were mainly IgG 1 and their binding was largely blocked by pathogenic anti-MOG antibodies derived from a spontaneous animal model of autoimmune encephalitis. The cross-sectional part of our study elaborated that anti-MOG Ig was present in about 25% of children with acute demyelination, but in none of the pediatric or adult controls. Sera from 4/62 (6%) adult patients with MS had anti-MOG IgG at low levels. CONCLUSIONS: The persistence or disappearance of antibodies to MOG may have prognostic relevance for acute childhood demyelination.
Asunto(s)
Autoanticuerpos/análisis , Encefalomielitis Aguda Diseminada/inmunología , Glicoproteína Asociada a Mielina/inmunología , Adolescente , Adulto , Unión Competitiva , Línea Celular , Niño , Preescolar , Estudios de Cohortes , Estudios Transversales , Femenino , Citometría de Flujo , Humanos , Inmunoglobulina G/análisis , Inmunoglobulinas/análisis , Lactante , Cinética , Estudios Longitudinales , Masculino , Esclerosis Múltiple/inmunología , Esclerosis Múltiple/metabolismo , Proteínas de la Mielina , Glicoproteína Mielina-Oligodendrócito , Estudios Prospectivos , TransfecciónRESUMEN
Granzymes are key components of the cytotoxic arm of the immune response, which play critical roles in eliminating host cells infected by intracellular pathogens and transformed cells. Although the induction of cell death is likely a central process underlying the function of these enzymes, little is known about whether granzymes use additional mechanisms to exert their antipathogen activity. This study identifies La, a phosphoprotein involved in multiple roles in cellular and viral RNA metabolism, as the first nonapoptotic substrate of granzyme H (gzmH), a cytotoxic granule protease that is constitutively expressed by NK cells. Cleavage of La by gzmH occurs at Phe-364 (P(1) site) and generates a COOH-terminal truncated form of La that loses nuclear localization and decreases HCV (hepatitis C virus)-internal ribosome entry site (IRES)-mediated translational activity. The ability of gzmH to cleave host proteins involved in essential viral functions provides a novel mechanism by which granzymes can mediate direct antiviral activities.
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Autoantígenos/metabolismo , Granzimas/metabolismo , Hepacivirus/genética , Biosíntesis de Proteínas , Ribonucleoproteínas/metabolismo , Secuencia de Aminoácidos , Línea Celular , Citoplasma/metabolismo , Células HeLa , Humanos , Células Asesinas Naturales , Datos de Secuencia Molecular , Transporte de Proteínas , Ribonucleoproteínas/antagonistas & inhibidores , Antígeno SS-BRESUMEN
Antineutrophil cytoplasmic antibodies (cANCA) against conformational epitopes ofproteinase 3 (PR3) are regarded as an important pathogenic marker in Wegener's granulomatosis (WG). Hence, PR3-based antibody binding assays are widely used for diagnosis and monitoring of the disease. Purification of the native catalytically active serine protease from granulocytes, however, is relatively inefficient, time-consuming and technically demanding. Conformational changes, partial aggregation, denaturation during purification and contaminations with inhibitors or other proteins from plasma and granulocytes, can affect the quality and comparability of PR3-based cANCA determinations. Alternative production of the human PR3 autoantigen by recombinant technologies offers several advantages over the natural antigen, but the complexity and operating expense of these procedures have, so far, delayed the development of new clinical tests. Correct posttranslational processing, conformational identity and antigen stability can be achieved by the expression of PR3 in Sf9 insect cells and in mammalian hosts, HEK293 and HMC-1. Subsequent purification and immobilization of the recombinant antigen is furthermore simplified by the attachment of short carboxy-terminal peptide tags. In contrast to conventional capture techniques with murine monoclonal antibodies, tag-based immobilization of the recombinant antigen does not mask portions of the PR3 surface and improves the efficacy of antigen coating. Moreover, recombinant PR3 variants will have a great potential to study individual anti-PR3 responses and will advance the development of new epitope-based therapeutics.
Asunto(s)
Autoantígenos/biosíntesis , Mieloblastina/biosíntesis , Proteínas Recombinantes/biosíntesis , Secuencia de Aminoácidos , Autoantígenos/química , Técnica del Anticuerpo Fluorescente , Expresión Génica , Humanos , Datos de Secuencia Molecular , Mieloblastina/química , Estructura Secundaria de Proteína , Proteínas Recombinantes/químicaRESUMEN
Detailed analyses of 20 patients with sporadic neurofibromatosis type 1 (NF1) microdeletions revealed an unexpected high frequency of somatic mosaicism (8/20 [40%]). This proportion of mosaic deletions is much higher than previously anticipated. Of these deletions, 16 were identified by a screen of unselected patients with NF1. None of the eight patients with mosaic deletions exhibited the mental retardation and facial dysmorphism usually associated with NF1 microdeletions. Our study demonstrates the importance of a general screening for NF1 deletions, regardless of a special phenotype, because of a high estimated number of otherwise undetected mosaic NF1 microdeletions. In patients with mosaicism, the proportion of cells with the deletion was 91%-100% in peripheral leukocytes but was much lower (51%-80%) in buccal smears or peripheral skin fibroblasts. Therefore, the analysis of other tissues than blood is recommended, to exclude mosaicism with normal cells in patients with NF1 microdeletions. Furthermore, our study reveals breakpoint heterogeneity. The classic 1.4-Mb deletion was found in 13 patients. These type I deletions encompass 14 genes and have breakpoints in the NF1 low-copy repeats. However, we identified a second major type of NF1 microdeletion, which spans 1.2 Mb and affects 13 genes. This type II deletion was found in 8 (38%) of 21 patients and is mediated by recombination between the JJAZ1 gene and its pseudogene. The JJAZ1 gene, which is completely deleted in patients with type I NF1 microdeletions and is disrupted in deletions of type II, is highly expressed in brain structures associated with learning and memory. Thus, its haploinsufficiency might contribute to mental impairment in patients with constitutional NF1 microdeletions. Conspicuously, seven of the eight mosaic deletions are of type II, whereas only one was a classic type I deletion. Therefore, the JJAZ1 gene is a preferred target of strand exchange during mitotic nonallelic homologous recombination. Although type I NF1 microdeletions occur by interchromosomal recombination during meiosis, our findings imply that type II deletions are mediated by intrachromosomal recombination during mitosis. Thus, NF1 microdeletions acquired during mitotic cell divisions differ from those occurring in meiosis and are caused by different mechanisms.
Asunto(s)
Eliminación de Gen , Mosaicismo , Proteínas de Neoplasias/genética , Neurofibromatosis 1/genética , Recombinación Genética , Factores de Transcripción/genética , Adolescente , Adulto , Anciano , Alelos , Animales , Encéfalo/metabolismo , División Celular , Línea Celular , Niño , Facies , Femenino , Marcadores Genéticos , Genotipo , Humanos , Hibridación in Situ , Hibridación Fluorescente in Situ , Discapacidad Intelectual/genética , Leucocitos/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Persona de Mediana Edad , Mitosis , Modelos Genéticos , Datos de Secuencia Molecular , Linaje , Fenotipo , Reacción en Cadena de la Polimerasa , ARN/metabolismo , Análisis de Secuencia de ADNAsunto(s)
Eliminación de Gen , Mosaicismo/genética , Proteínas de Neoplasias , Neurofibromatosis 1/genética , Recombinación Genética , Factores de Transcripción/genética , Adulto , Animales , Línea Celular , Niño , Salud de la Familia , Resultado Fatal , Femenino , Haplotipos/genética , Humanos , Hibridación Fluorescente in Situ , Mitosis/genética , Neurofibromatosis 1/patología , LinajeRESUMEN
Homologous recombination between poorly characterized regions flanking the NF1 locus causes the constitutional loss of approximately 1.5 Mb from 17q11.2 covering > or =11 genes in 5%-20% of patients with neurofibromatosis type 1 (NF1). To elucidate the extent of microheterogeneity at the deletion boundaries, we used single-copy DNA fragments from the extreme ends of the deleted segment to perform FISH on metaphase chromosomes from eight patients with NF1 who had large deletions. In six patients, these probes were deleted, suggesting that breakage and fusions occurred within the adjacent highly homologous sequences. Reexamination of the deleted region revealed two novel functional genes FLJ12735 (AK022797) and KIAA0653-related (WI-12393 and AJ314647), the latter of which is located closest to the distal boundary and is partially duplicated. We defined the complete reading frames for these genes and two expressed-sequence tag (EST) clusters that were reported elsewhere and are associated with the markers SHGC-2390 and WI-9521. Hybrid cell lines carrying only the deleted chromosome 17 were generated from two patients and used to identify the fusion sequences by junction-specific PCRs. The proximal breakpoints were found between positions 125279 and 125479 in one patient and within 4 kb of position 143000 on BAC R-271K11 (AC005562) in three patients, and the distal breakpoints were found at the precise homologous position on R-640N20 (AC023278). The interstitial 17q11.2 microdeletion arises from unequal crossover between two highly homologous WI-12393-derived 60-kb duplicons separated by approximately 1.5 Mb. Since patients with the NF1 large-deletion syndrome have a significantly increased risk of neurofibroma development and mental retardation, hemizygosity for genes from the deleted region around the neurofibromin locus (CYTOR4, FLJ12735, FLJ22729, HSA272195 (centaurin-alpha2), NF1, OMGP, EVI2A, EVI2B, WI-9521, HSA272196, HCA66, KIAA0160, and WI-12393) may contribute to the severe phenotype of these patients.
Asunto(s)
Deleción Cromosómica , Cromosomas Humanos Par 17 , Genes de Neurofibromatosis 1/genética , Neurofibromatosis 1/genética , Secuencia de Bases , Células Cultivadas , Mapeo Cromosómico , ADN/análisis , Evolución Molecular , Duplicación de Gen , Humanos , Hibridación Fluorescente in Situ , Cariotipificación , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Homología de Secuencia de Ácido NucleicoRESUMEN
Homing behavior and function of autoimmune CD4+ T cells in vivo was analyzed before and during EAE, using MBP-specific T cells retrovirally engineered to express the gene of green fluorescent protein. The cells migrate from parathymic lymph nodes to blood and to the spleen. Preceding disease onset, large numbers of effector cells invade the CNS, with only negligible numbers left in the periphery. In early EAE, most (>90%) infiltrating CD4+ cells were effector cells. Migratory effector cells downregulate activation markers (CD25, OX-40) but upregulate several chemokine receptors and adsorb MHC class II on their membranes. Within the CNS, the effector cells are reactivated, with upregulated proinflammatory cytokines and downmodulated T cell receptor-associated structures, presumably reflecting autoantigen recognition in situ.
Asunto(s)
Linfocitos T CD4-Positivos/inmunología , Movimiento Celular , Encefalomielitis Autoinmune Experimental/inmunología , Animales , Sistema Nervioso Central/inmunología , Proteínas Fluorescentes Verdes , Antígenos de Histocompatibilidad Clase II/inmunología , Inyecciones Intraperitoneales , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/metabolismo , Activación de Linfocitos/inmunología , Proteína Básica de Mielina/genética , Proteína Básica de Mielina/metabolismo , Ratas , Ratas Endogámicas LewRESUMEN
A cosmid/bacterial artificial chromosome (BAC) contiguous (contig) map of human chromosome (HSA) 19p13.3 has been constructed, and over 50 genes have been localized to the contig. Genes and anonymous ESTs from approximately 4000 kb of human 19p13.3 were placed on the central mouse chromosome 10 map by genetic mapping and pulsed-field gel electrophoresis (PFGE) analysis. A region of approximately 2500 kb of HSA 19p13.3 is collinear to mouse chromosome (MMU) 10. In contrast, the adjacent approximately 1200 kb are inverted. Two genes are located in a 50-kb region after the inversion on MMU 10, followed by a region of homology to mouse chromosome 17. The synteny breakpoint and one of the inversion breakpoints has been localized to sequenced regions in human <5 kb in size. Both breakpoints are rich in simple tandem repeats, including (TCTG)n, (CT)n, and (GTCTCT)n, suggesting that simple repeat sequences may be involved in chromosome breaks during evolution. The overall size of the region in mouse is smaller, although no large regions are missing. Comparing the physical maps to the genetic maps showed that in contrast to the higher-than-average rate of genetic recombination in gene-rich telomeric region on HSA 19p13.3, the average rate of recombination is lower than expected in the homologous mouse region. This might indicate that a hot spot of recombination may have been lost in mouse or gained in human during evolution, or that the position of sequences along the chromosome (telomeric compared to the middle of a chromosome) is important for recombination rates.
Asunto(s)
Rotura Cromosómica/genética , Cromosomas Humanos Par 19/genética , Evolución Molecular , Mapeo Físico de Cromosoma , Animales , Inversión Cromosómica , Cromosomas Bacterianos/genética , Cósmidos/genética , Electroforesis en Gel de Campo Pulsado , Femenino , Marcadores Genéticos/genética , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Mutantes Neurológicos , Secuencias Repetitivas de Ácidos Nucleicos , Homología de Secuencia de Ácido NucleicoRESUMEN
Segmental neurofibromatosis (NF) is generally thought to result from a postzygotic NF1 (neurofibromatosis type 1) gene mutation. However, this has not yet been demonstrated at the molecular level. Using fluorescence in situ hybridisation (FISH) we identified an NF1 microdeletion in a patient with segmental NF in whom café-au-lait spots and freckles are limited to a single body region. The mutant allele was present in a mosaic pattern in cultured fibroblasts from a café-au-lait spot lesion, but was absent in fibroblasts from normal skin as well as in peripheral blood leukocytes. These findings prove the hypothesis that the molecular basis of segmental cutaneous NF is a mutation in the NF1 gene and that the regional distribution of manifestations reflects different cell clones, commensurate with the concept of somatic mosaicism.
Asunto(s)
Eliminación de Gen , Mosaicismo , Proteínas del Tejido Nervioso/genética , Neurofibromatosis/genética , Adolescente , Humanos , Hibridación Fluorescente in Situ , Masculino , Mutación , Neurofibromina 1 , FenotipoRESUMEN
Large deletions of the NF1 locus occur in 5 to 10% of patients with neurofibromatosis and are commonly associated with specific additional abnormalities characterized by mental retardation, dysmorphic features, and intellectual impairment. To characterize the extent of codeleted genes we constructed a long-range physical BAC/PAC map around the NF1 locus between D17S117 and D17S57 and determined the deletion boundaries in seven unrelated patients. Surprisingly, the proximal and distal breakpoints in five of seven patients fall at almost identical positions, resulting in the loss of at least 11 functional genes. Five of six patients investigated showed a de novo deletion on the maternally derived chromosome. Since D17S117 and D17S57 were previously reported as the outer limits for the great majority of NF1 deletions, we suggest that most NF1 patients with deletion of the entire NF1 gene are hemizygous for the same set of at least 10 additional genes, including SHGC-37343, SHGC-2390, SHGC-34232, OMG, EVI2B, EVI2A, WI-9521, WI-6742, SHGC-34334, and KIAA0160, and thus present with a relatively uniform clinical phenotype.
Asunto(s)
Eliminación de Gen , Proteínas del Tejido Nervioso/genética , Técnicas de Cultivo de Célula , Rotura Cromosómica , Cromosomas Humanos Par 17 , Mapeo Contig , Citogenética , Exones , Etiquetas de Secuencia Expresada , Salud de la Familia , Haplotipos , Humanos , Datos de Secuencia Molecular , Neurofibromatosis/genética , Neurofibromatosis/patología , Neurofibromina 1 , Fenotipo , Mapeo Físico de Cromosoma , Lugares Marcados de SecuenciaRESUMEN
Autosomal recessive limb-girdle muscular dystrophies (AR LGMDs) are a genetically heterogeneous group of disorders that affect mainly the proximal musculature. There are eight genetically distinct forms of AR LGMD, LGMD 2A-H (refs 2-10), and the genetic lesions underlying these forms, except for LGMD 2G and 2H, have been identified. LGMD 2A and LGMD 2B are caused by mutations in the genes encoding calpain 3 (ref. 11) and dysferlin, respectively, and are usually associated with a mild phenotype. Mutations in the genes encoding gamma-(ref. 14), alpha-(ref. 5), beta-(refs 6,7) and delta (ref. 15)-sarcoglycans are responsible for LGMD 2C to 2F, respectively. Sarcoglycans, together with sarcospan, dystroglycans, syntrophins and dystrobrevin, constitute the dystrophin-glycoprotein complex (DGC). Patients with LGMD 2C-F predominantly have a severe clinical course. The LGMD 2G locus maps to a 3-cM interval in 17q11-12 in two Brazilian families with a relatively mild form of AR LGMD (ref. 9). To positionally clone the LGMD 2G gene, we constructed a physical map of the 17q11-12 region and refined its localization to an interval of 1.2 Mb. The gene encoding telethonin, a sarcomeric protein, lies within this candidate region. We have found that mutations in the telethonin gene cause LGMD 2G, identifying a new molecular mechanism for AR LGMD.
Asunto(s)
Cromosomas Humanos Par 17 , Proteínas Musculares/genética , Distrofias Musculares/genética , Secuencia de Aminoácidos , Secuencia de Bases , Mapeo Cromosómico , Conectina , Exones , Femenino , Genes Recesivos , Marcadores Genéticos , Humanos , Intrones , Masculino , Repeticiones de Microsatélite , Datos de Secuencia Molecular , Proteínas Musculares/química , Distrofias Musculares/clasificación , Núcleo Familiar , Linaje , Regiones Promotoras Genéticas , Sarcómeros/genética , Sarcómeros/metabolismo , Alineación de SecuenciaRESUMEN
Neutrophil elastase, proteinase-3, and azurocidin are primary components of neutrophil azurophilic granules and are encoded by closely linked genes (gene symbols ELA2, PRTN3, and AZU1, respectively) in a region of approximately 50 kb. These genes are coordinately expressed in a granulocyte-specific fashion, but the mechanisms defining this pattern of expression are unknown. To understand the role of chromatin organization in governing the expression of ELA2, PRTN3, and AZU1, we mapped this region of chromosome 19 and identified the adipsin (complement factor D) gene in proximity to the 3' end of ELA2. We then examined the changes in chromatin structure at the locus which accompany myeloid cell differentiation and identified 17 DNase I hypersensitive sites (DHS 1 to 17) in U-937 cells, an early myelomonocytic cell line expressing high levels of neutrophil elastase. Chemically induced differentiation and concomitant downregulation of AZU1, PRTN3, and ELA2 transcription in U-937 cells is not accompanied by changes in the DHS-pattern. Mature neutrophils, however, do not carry any of these hypersensitive sites, indicating a large degree of chromatin remodeling at this locus accompanying terminal granulocytic differentiation. Sixteen of the 17 DHS identified in U-937 cells are also present in the HL-60 myelomonocytic cell line. Hematopoietic cell lines representing the early erythroid and lymphocyte lineages, and a nonhematopoietic cell line display a subset of the hypersensitive sites. The altered chromatin structure specific to cells that actively transcribe the AZU1-PRTN3-ELA2 genes suggests that chromatin reorganization is an important mechanism regulating the myeloid-specific transcription of this gene cluster.
Asunto(s)
Cromatina/genética , Cromosomas Humanos Par 19 , Elastasa de Leucocito/genética , Leucopoyesis/genética , Cromatina/ultraestructura , Mapeo Cromosómico , Regulación de la Expresión Génica , Ligamiento Genético , Células HL-60 , Humanos , Células U937RESUMEN
AIM: To examine the membranous and nuclear distribution of beta catenin in the epithelial cells of gut polyps from Peutz-Jeghers syndrome and juvenile polyposis in comparison with other types of polyps and tumours. METHODS: Immunohistochemistry for beta catenin and proliferation markers was performed on conventional paraffin sections. Immunohistological staining was carried out on Peutz-Jeghers syndrome polyps from four different families, on juvenile polyposis polyps from two different families, on solitary juvenile polyps, and on hyperplastic polyps. The immunohistochemistry was evaluated qualitatively in relation to defined areas of the polyps. RESULTS: All polyps from the hamartomatous polyposis syndromes (Peutz-Jeghers syndrome and juvenile polyposis) showed nuclear localization of beta catenin in some epithelial cell nuclei. In Peutz-Jeghers syndrome polyps beta catenin positive nuclei were seen at the base of the deep crypt infoldings. In juvenile polyposis polyps and in some solitary juvenile polyps they were found in irregularly distributed cryptal epithelial cells corresponding to the proliferative compartments. Normal mucosa of the gut and hyperplastic polyps of the colon do not show nuclear staining for beta catenin. CONCLUSIONS: The dysregulation of cellular beta catenin distribution is not only a phenomenon of adenoma formation and adenoma progression in the colon--it is at least focally present in polyps of the hamartomatous type and is related to the proliferation zones of these polyps. The nuclear translocation of beta catenin most probably reflects a disturbed beta catenin metabolism. In view of the different functions of beta catenin during development and cell differentiation, the nuclear translocation of beta catenin is likely to be an important factor in enhanced cell proliferation which escapes local control mechanisms.
Asunto(s)
Poliposis Adenomatosa del Colon/metabolismo , Cadherinas/metabolismo , Proteínas del Citoesqueleto/metabolismo , Síndrome de Peutz-Jeghers/metabolismo , Transactivadores , Adenocarcinoma/metabolismo , Neoplasias Colorrectales/metabolismo , Humanos , Técnicas para Inmunoenzimas , Mucosa Intestinal/metabolismo , Pólipos Intestinales/metabolismo , Proteínas de Neoplasias/metabolismo , beta CateninaRESUMEN
Tryptase-like activities of T and NK cells contribute to the induction of target cell apoptosis, but only granzyme A (GzmA) has been shown to exhibit Z-Lys-SBzl esterase activity in murine T cells. GzmA-deficient mice exhibit residual Z-Lys-SBzl hydrolyzing activity and almost normal levels of lymphocyte-mediated cytotoxicity. Here we report the cloning and biochemical characterization of recombinant mouse granzyme K (GzmK). The purified murine protein shows Z-Lys-SBzl hydrolyzing activity and is inhibited by bikunin, the light chain of inter-alpha-trypsin inhibitor, like the human homolog. We conclude that GzmK expressed by GzmA-deficient T cells accounts for the remaining Z-Lys-SBzl activity. Functional similarities between GzmA and GzmK may explain the subtle immunological deficits observed in GzmA-deficient mice.