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1.
Dig Liver Dis ; 40(9): 731-6, 2008 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-18387860

RESUMEN

BACKGROUND: The aetiology of ulcerative colitis is inadequately understood, and drug therapy has been empirical rather than based on sound understanding of disease aetiology. This has been a major factor for refractoriness and adverse drug effects as additional complications. However, ulcerative colitis by its very nature is exacerbated and perpetuated by inflammatory cytokines, which are released by peripheral granulocytes and monocytes as well. Additionally, active ulcerative colitis is often associated with elevated peripheral granulocytes and monocytes with activation behaviour and are found in vast numbers within the colonic mucosa. Hence, from the clinicopathologic viewpoint, granulocytes and monocytes are appropriate targets for therapy in ulcerative colitis. Based on this thinking, an Adacolumn has been developed for depleting excess granulocytes and monocytes by adsorption. METHODS: By colonoscopy, biopsy and histology, we investigated the impact of granulocyte and monocyte adsorption (GMA) on the mucosal level of granulocytes and monocytes in patients with active ulcerative colitis. Forty-five patients (26 steroid naïve and 19 steroid-dependent), mean age 44.7 yr, were included. Twenty patients had total colitis and 25 had left-sided colitis. Each patient was given up to 11 GMA sessions over 12 weeks. No patient received additional medications within 4 weeks (steroid) to 8 weeks (other immunosuppressants) prior to entry or during the GMA course. Colonoscopy together with biopsy was done at entry and within 2 weeks after the last GMA session. RESULTS: At entry, the mean clinical activity index was 12.6; range 10-16. A total of 400 colonic biopsies were examined, which revealed massive infiltration of the colonic mucosa by granulocytes, and GMA was associated with striking reduction of granulocytes in the mucosa. At week 12, 33 of 45 patients (73.3%, P<0.01) had achieved clinical remission (the mean clinical activity index

Asunto(s)
Colitis Ulcerosa/terapia , Granulocitos/inmunología , Leucaféresis/métodos , Monocitos/inmunología , Esteroides/uso terapéutico , Adulto , Biopsia con Aguja , Estudios de Cohortes , Colitis Ulcerosa/diagnóstico , Colitis Ulcerosa/inmunología , Colitis Ulcerosa/mortalidad , Colonoscopía , Citocinas/inmunología , Citocinas/metabolismo , Femenino , Estudios de Seguimiento , Granulocitos/metabolismo , Humanos , Inmunohistoquímica , Mucosa Intestinal/inmunología , Mucosa Intestinal/patología , Masculino , Persona de Mediana Edad , Monocitos/metabolismo , Probabilidad , Medición de Riesgo , Sensibilidad y Especificidad , Índice de Severidad de la Enfermedad , Tasa de Supervivencia , Resultado del Tratamiento
2.
Int J Clin Pharmacol Res ; 25(3): 101-5, 2005.
Artículo en Inglés | MEDLINE | ID: mdl-16366417

RESUMEN

Methotrexate (MTX)-induced acute lung injury developed in a female patient with rheumatoid arthritis. She was successfully treated with high-dose glucocorticoid therapy. During her hospital stay, the serum concentration of surfactant protein (SP)-D, which was markedly elevated on admission, was finally normalized and the disease resolved. However, the serum concentration of Klebs von den Lungen (KL)-6 remained high. Although the mechanisms of lung injury by MTX have not been well defined, serial measurements of serum SPD might be useful for the clinical evaluation of drug-induced acute lung injury.


Asunto(s)
Antirreumáticos/efectos adversos , Artritis Reumatoide/tratamiento farmacológico , Enfermedades Pulmonares/inducido químicamente , Metotrexato/efectos adversos , Enfermedad Aguda , Anciano , Antirreumáticos/uso terapéutico , Glucocorticoides/uso terapéutico , Humanos , Enfermedades Pulmonares/diagnóstico por imagen , Enfermedades Pulmonares/tratamiento farmacológico , Masculino , Metotrexato/uso terapéutico , Tomografía Computarizada por Rayos X , Resultado del Tratamiento
3.
Mod Rheumatol ; 12(2): 144-7, 2002 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24383902

RESUMEN

Abstract In the Sauvé-Kapandji (S-K) procedure for rheumatoid wrist reconstruction, the distal end of the ulna is fixed to the radius with screws. Recently, absorbable screws have increasingly been used instead of metal ones. However, the clinical usefulness of absorbable screws in S-K procedures for rheumatoid patients is still unknown. The purpose of this article is to evaluate the effect of absorbable screws in this procedure by comparing their clinical results with those of metal screws. Poly-l-lactic acid (PLLA) absorbable screws were used in 23 wrists, and metal screws were used in 20 wrists. We evaluated the presence of general or local reactions to PLLA, the stability of the ulnar head, the time to bone union, changes in the shape of the distal ulna, and the presence of bone resorption around the screws. There were no complications with the use of PLLA screws, and their fixation stability was adequate to form sufficient bone union. In five cases in the metal screw group, bone resorption around the screws occurred between 1 and 2 years after surgery. Bone resorption around the PLLA screws was not observed. We conclude that absorbable screws may be more useful than metal screws in the S-K procedure for rheumatoid wrist reconstruction.

4.
Appl Opt ; 40(30): 5495-502, 2001 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-18364834

RESUMEN

In recent years there has been increasing interest in the use of polarization for imaging objects in a cluttered environment. Examples are optical imaging through clouds, optical detection of objects in a biological medium, and microwave detection of objects in clutter. We extend previous studies of continuous-wave scattering to pulse-polarization scattering in discrete scatterers. We solve the time-dependent vector radiative transfer equation for a plane-parallel medium by using Mie scattering and the discrete ordinates method. The time-dependent degree of polarization and cross-polarization discrimination are calculated and verify the advantages of circular over linear polarization in maintaining greater copolarized components rather than cross-polarized components.

5.
Neurol Med Chir (Tokyo) ; 40(11): 589-93, 2000 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-11109798

RESUMEN

A 16-year-old male presented with a large, solid hemangioblastoma located in the upper cervical cord manifesting as hyperactive reflexes, subtle weakness, and diminished position sense in all extremities. Neuroimaging studies indicated venous congestion due to arteriovenous shunt through the tumor. Preoperative embolization was accomplished without morbidity, and resulted in marked devascularization of the tumor and elimination of an early filling vein. Four days after embolization, the tumor was totally excised without excessive intraoperative bleeding. His neurological deficits gradually improved after surgery. Preoperative embolization is a valuable adjunct to surgical excision of large intramedullary hemangioblastomas, especially those associated with arteriovenous shunt, as cord dysfunction related to venous congestion and the risk of torrential intraoperative bleeding are reduced.


Asunto(s)
Embolización Terapéutica , Hemangioblastoma/irrigación sanguínea , Hemangioblastoma/terapia , Procedimientos Neuroquirúrgicos , Neoplasias de la Médula Espinal/irrigación sanguínea , Neoplasias de la Médula Espinal/terapia , Adolescente , Vértebras Cervicales , Terapia Combinada , Embolización Terapéutica/métodos , Hemangioblastoma/diagnóstico , Hemangioblastoma/cirugía , Humanos , Masculino , Cuidados Preoperatorios/métodos , Recuperación de la Función , Neoplasias de la Médula Espinal/diagnóstico , Neoplasias de la Médula Espinal/cirugía , Resultado del Tratamiento
6.
Br J Plast Surg ; 53(7): 607-11, 2000 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-11000078

RESUMEN

The effects of chitin sheet interposition with and without brain gangliosides on the regeneration of hypoglossal nerve fibres was studied in the rat following resection of a 5mm length of the nerve. At 10 weeks after operation, the number of horseradish peroxidase (HRP)-labelled motor neurones, indicative of the axonal repair process, on the side treated with chitin and gangliosides was higher than on the control side (where 5mm of the nerve was simply resected). The ratios of HRP-positive neurones in the right hypoglossal nucleus (treated side)/left hypoglossal nucleus (intact side) was 0 in the 5mm-resected group, 53% in the chitin-grafted group, 88% in the ganglioside (0.2 microg)-injected group, 90% in the ganglioside (2 microg)-injected group, 91% in the chitin with ganglioside (0.2mg)-injected group, 91% in the chitin with ganglioside (2 microg)-injected group and 85% in the autograft group, respectively. There were significant differences between the 5 mm-resected group and chitin-grafted group, ganglioside-injected group, chitin with ganglioside group and autograft group, and between the chitin-grafted group and ganglioside-injected, chitin with ganglioside and autograft groups (P< 0.005, respectively). Our results indicated that the use of chitin and gangliosides stimulated the regeneration of severed motor nerve fibres. These findings suggest that chitin and gangliosides might be therapeutically useful for treatment of neuronal degeneration.


Asunto(s)
Quitina/uso terapéutico , Gangliósidos/uso terapéutico , Nervio Hipogloso/fisiología , Regeneración Nerviosa/efectos de los fármacos , Animales , Evaluación Preclínica de Medicamentos , Femenino , Peroxidasa de Rábano Silvestre , Neuronas Motoras/fisiología , Ratas , Ratas Wistar
7.
Cell Mol Neurobiol ; 20(5): 529-40, 2000 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-10930130

RESUMEN

1. Chitin is known to promote skin wound healing. In this study, chitin, prepared from Zuwai crab shell, was used as a bridge between the proximal and distal stumps of cut hypoglossal nerves in shrews. We compared the effects of chitin on the regeneration of transected right hypoglossal nerve axons, with those of porcine dermis, bovine dermal aterocollagen, and autologous nerve bundles. 2. To assess the survival of neurones, the size of neuronal cell body, and number of motoneurones were determined in the absence of any bridged material and in the presence of porcine dermis, bovine dermal aterocollagen, chitin, or autologous nerve bundles as a bridge. 3. Our results revealed a significantly better outcome in chitin and autologous nerve bridged groups; the size of neuronal cell body and number of hypoglossal neurones were higher than in the other groups. Chitin also enhanced the regeneration of neurones; the number of horseradish peroxide positive neurones indicative of repaired axonal processes was significantly higher in chitin and autologous nerve-bridged groups than in other groups. 4. Our results demonstrated that the use of chitin sheet or autograft successfully prevented the death of severed neurones and promoted the regeneration of the lesioned nerve. Although the mechanisms underlying the effects of chitin are still unknown, chitin seems to be a potentially useful biocompatible material for nerve repair and regeneration.


Asunto(s)
Muerte Celular/fisiología , Quitina/farmacología , Nervio Hipogloso/citología , Nervio Hipogloso/fisiología , Regeneración Nerviosa/efectos de los fármacos , Animales , Axotomía , Recuento de Células , Tamaño de la Célula , Peroxidasa de Rábano Silvestre , Nervio Hipogloso/cirugía , Ensayo de Materiales , Neuronas Motoras/citología , Neuronas Motoras/fisiología , Musarañas , Cicatrización de Heridas/efectos de los fármacos
8.
Kurume Med J ; 47(2): 147-9, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-10948652

RESUMEN

While alcohol abuse is a possible etiologic factor in osteonecrosis in the femoral head (ON), the relationship between alcoholic liver dysfunction and ON is uncertain. Among 336 patients with alcoholic liver dysfunction who had radiographic examination of the hip at two hospitals for alcohol abuse treatment in southern Japan, the records for 291 men and 1 woman (mean age, 47.8 years; range, 24 to 72 years) had adequate information available concerning daily and cumulative alcohol intake, duration of intake, serum concentrations of liver enzymes, and platelet count. These variables were investigated for any correlation between the 8 patients with radiographic evidence of ON and the 284 without. Liver biopsy was performed in 223 patients. Except for alanine aminotransferase, liver enzyme concentrations were significantly lower in patients with ON than in those without. Histologically, 2 patients with ON were diagnosed with cirrhosis; 1 with pre-cirrhotic changes; and 2 with fibrosis. These results suggested that ON occurred in the late stages of liver disease when serum enzyme concentrations had returned to normal or were only mildly elevated.


Asunto(s)
Alcoholismo/complicaciones , Necrosis de la Cabeza Femoral/etiología , Adulto , Anciano , Alcoholismo/patología , Femenino , Necrosis de la Cabeza Femoral/patología , Humanos , Masculino , Persona de Mediana Edad
9.
J Neurosurg ; 92(6): 1045-9, 2000 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10839269

RESUMEN

The authors describe the case of a 51-year-old man with a Type 1 dural arteriovenous fistula (AVF) located at the junction of the transverse and sigmoid sinuses. The dural AVF developed after the patient underwent a craniotomy for an acute extradural hematoma. The patient suffered pulsatile tinnitus 3 months after surgery. After several attempts at transarterial embolization (TAE), the venous channel located close to the skull fracture was accessed via a transfemoral-transvenous approach and was embolized by administering a liquid nonadhesive agent. Successful embolization of the dural AVF was achieved both clinically and radiologically without causing considerable hemodynamic alterations. This procedure, either alone or combined with TAE, would seem to be an alternative treatment for dural AVFs in this location, without causing compromise of flow within the affected sinuses, when selective venous access is available.


Asunto(s)
Fístula Arteriovenosa/terapia , Duramadre/irrigación sanguínea , Embolización Terapéutica/métodos , Enfermedad Aguda , Fístula Arteriovenosa/diagnóstico , Fístula Arteriovenosa/etiología , Encefalopatías/cirugía , Angiografía Cerebral , Senos Craneales , Craneotomía/efectos adversos , Hematoma/cirugía , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Acúfeno/etiología , Tomografía Computarizada por Rayos X
10.
Neurol Med Chir (Tokyo) ; 40(3): 169-72, 2000 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-10842488

RESUMEN

A 21-year-old female presented with an unusual case of posterior fossa arteriovenous malformation (AVM) associated with ipsilateral persistent primitive trigeminal artery (PPTA), manifesting as intraparenchymal hemorrhage involving both the brain stem and the left cerebellar hemisphere. The presenting symptoms were compatible with Wallenberg's syndrome and Foville's syndrome on the left side. She was initially treated conservatively, and subsequently with transarterial embolization followed by stereotactic radiosurgery. This case combined the rare association of posterior fossa AVM and PPTA, with the clinical presentation of intraparenchymal hemorrhage causing both Wallenberg's syndrome and Foville's syndrome.


Asunto(s)
Malformaciones Arteriovenosas/cirugía , Cerebelo/irrigación sanguínea , Hemorragia Cerebral/cirugía , Nervio Trigémino/irrigación sanguínea , Adulto , Malformaciones Arteriovenosas/diagnóstico por imagen , Tronco Encefálico/irrigación sanguínea , Hemorragia Cerebral/diagnóstico por imagen , Terapia Combinada , Fosa Craneal Posterior/irrigación sanguínea , Embolización Terapéutica , Femenino , Humanos , Síndrome Medular Lateral/diagnóstico por imagen , Síndrome Medular Lateral/cirugía , Radiografía , Radiocirugia
11.
Glycobiology ; 9(11): 1247-52, 1999 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-10536040

RESUMEN

We have examined the time course of the neuronal death and regeneration of rat axotomized hypoglossal nerve with various conditions of the nerve resection, and established a useful system to measure neurotrophic activities of bioactive substances. In this system, neuronal death can be evaluated by counting surviving neurons in the nucleus of hypoglossal neuron at the brain stem, and the degree of the regeneration can be measured by counting horseradish peroxidase-positive cells at the same region after injection of horseradish peroxidase into tongue. Using this system, the effects of brain gangliosides on rat hypoglossal nerve regeneration following 5 mm transection were examined. The addition of a ganglioside mixture from bovine brain as well as the autograft strongly prevented the death of neurons and promoted the regeneration of the lesioned nerve at 10 weeks after the operation. Further analyses on the dose effects and injection sites of gangliosides were performed. Although the mechanisms of the neurotrophic effects of the gangliosides are unknown, the therapeutic application of gangliosides for neuronal degeneration is a promising approach.


Asunto(s)
Apoptosis/efectos de los fármacos , Química Encefálica , Desnervación , Gangliósidos/farmacología , Traumatismos del Nervio Hipogloso , Neuronas Motoras/efectos de los fármacos , Regeneración Nerviosa/efectos de los fármacos , Fármacos Neuroprotectores/farmacología , Degeneración Retrógrada , Animales , Bovinos , Recuento de Células , Relación Dosis-Respuesta a Droga , Gangliósidos/administración & dosificación , Gangliósidos/aislamiento & purificación , Peroxidasa de Rábano Silvestre , Nervio Hipogloso/efectos de los fármacos , Nervio Hipogloso/fisiología , Inyecciones , Neuronas Motoras/patología , Fármacos Neuroprotectores/administración & dosificación , Fármacos Neuroprotectores/aislamiento & purificación , Ratas , Ratas Wistar , Lengua/inervación
12.
Rheumatology (Oxford) ; 38(8): 714-20, 1999 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-10501417

RESUMEN

OBJECTIVE: To investigate the effect of the synovial fluid from knee joints of rheumatoid arthritis (RA) patients with different severities of joint destruction on osteoclastogenesis and bone resorption. METHODS: Synovial fluid was harvested from the knee joints of 59 RA patients and 37 ostcoarthritis (OA) patients. RA patients with Larsen's knee grade 1-3 were classified as mild RA (n = 30) and those with grade 4 or 5 as severe RA (n = 29). Cytokine concentrations in synovial fluid were measured by ELISA. Osteoclastogenesis was measured by tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cell (MNC) formation in a co-culture of mouse osteoblastic cells and bone marrow cells, and bone resorption by 45Ca release from pre-labelled cultured neonatal mouse calvariae. RESULTS: The synovial fluid of severe RA patients significantly stimulated TRAP-positive MNC formation and 45Ca release compared to those of mild RA and OA patients. Among the bone-resorptive cytokines fibroblast growth factor-2 (FGF-2), tumour necrosis factor alpha (TNF-alpha), interleukin-1alpha (IL-1alpha), IL-6 and soluble IL-6 receptor (sIL-6R), only FGF-2 concentration in the synovial fluid was positively correlated to Larsen's grade, and severe RA patients showed significantly higher FGF-2 concentrations than mild RA patients. Osteoclastogenesis in a co-culture system which was stimulated by the synovial fluid of severe RA patients was significantly inhibited by a neutralizing antibody against FGF-2 and this inhibition was stronger than antibodies against other cytokines. CONCLUSION: The increase in endogenous FGF-2 levels in the synovial fluid of RA patients may play a role in the joint destruction by inducing osteoclastogenesis.


Asunto(s)
Artritis Reumatoide/fisiopatología , Resorción Ósea , Factor 2 de Crecimiento de Fibroblastos/fisiología , Animales , Artritis Reumatoide/metabolismo , Artritis Reumatoide/patología , Calcio/metabolismo , Diferenciación Celular/efectos de los fármacos , Técnicas de Cocultivo , Inhibidores de la Ciclooxigenasa/farmacología , Citocinas/metabolismo , Relación Dosis-Respuesta a Droga , Femenino , Factor 2 de Crecimiento de Fibroblastos/metabolismo , Humanos , Masculino , Ratones , Persona de Mediana Edad , Nitrobencenos/farmacología , Osteoartritis/metabolismo , Osteoartritis/patología , Osteoartritis/fisiopatología , Osteoclastos/citología , Osteoclastos/efectos de los fármacos , Sulfonamidas/farmacología , Líquido Sinovial/metabolismo
13.
Intern Med ; 37(9): 792-5, 1998 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-9804092

RESUMEN

A 66-year-old man was admitted with destructive arthropathy, and calcium pyrophosphate dihydrate was demonstrated in the synovial fluid specimen. He was found to have a hyponatremia. The serum sodium concentration was 121 mmol/l, plasma arginine vasopressin (AVP) 6.6 pmol/l, and serum interleukin (IL)-6 96 pg/l. The clinical findings suggest the diagnosis of syndrome of inappropriate secretion of antidiuretic hormone (SIADH). However, destructive arthropathy with increased values of C-reactive protein and IL-6 is the only background of SIADH in this patient. We suggest the possibility that IL-6 produced at inflammatory lesions may have stimulated an excessive release of AVP resulting in the hyponatremia and hypochloremia of SIADH.


Asunto(s)
Arginina Vasopresina/metabolismo , Artritis/complicaciones , Hiponatremia/etiología , Síndrome de Secreción Inadecuada de ADH/etiología , Anciano , Arginina Vasopresina/sangre , Artritis/fisiopatología , Proteína C-Reactiva/análisis , Pirofosfato de Calcio/análisis , Enfermedad Crónica , Humanos , Interleucina-6/sangre , Masculino , Líquido Sinovial/química
14.
Cardiovasc Drugs Ther ; 12(1): 3-12, 1998 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-9607127

RESUMEN

The effect of bezafibrate on plasma lipoproteins was investigated in Japanese familial combined hyperlipidemic patients with or without an impaired glucose tolerance accompanied by a low-density lipoprotein subclass, with the major gradient gel peak at a particle diameter of less than 25.5 nm. Bezafibrate treatment at a dose of 400 mg/d for 12 weeks produced an antiatherogenic effect on lipoprotein profiles, as reflected by a decrease in plasma triglyceride levels, an increase in plasma high-density lipoprotein-cholesterol levels, induction of the large-size subclass of low-density lipoprotein, and disappearance of intermediate-density lipoproteins. The plasma total and low-density lipoprotein-cholesterol-lowering effect of bezafibrate was significant in patients without impaired glucose tolerance but was not significant in patients with impaired glucose tolerance. Bezafibrate increased lipoprotein lipase activity and decreased the activity of cholesteryl ester transfer protein, both in patients with or without impaired glucose tolerance. There was no difference in the distribution of signal peptide insertion/deletion or Xbal polymorphisms of the apolipoprotein B gene in patients with or without impaired glucose tolerance. Mechanisms other than lipoprotein lipase, cholesteryl ester transfer protein activities, and an apolipoprotein B gene polymorphism may be responsible for the resistance to lowering of plasma total and low-density lipoprotein cholesterol levels with bezafibrate treatment in familial combined hyperlipidemic patients with impaired glucose tolerance.


Asunto(s)
Bezafibrato/uso terapéutico , Glicoproteínas , Hiperlipidemia Familiar Combinada/tratamiento farmacológico , Hipolipemiantes/uso terapéutico , Lipoproteínas/sangre , Adulto , Anciano , Apolipoproteínas B/genética , Proteínas Portadoras/sangre , Proteínas de Transferencia de Ésteres de Colesterol , Ésteres del Colesterol/sangre , Femenino , Prueba de Tolerancia a la Glucosa , Humanos , Hiperlipidemia Familiar Combinada/sangre , Hiperlipidemia Familiar Combinada/genética , Lipoproteína Lipasa/sangre , Lipoproteínas LDL/sangre , Masculino , Persona de Mediana Edad
15.
Cardiovasc Drugs Ther ; 12(1): 19-28, 1998 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-9607129

RESUMEN

The proliferation of vascular smooth muscle cells (VSMCs) plays an important role in the formation of atherosclerotic lesions and restenosis after angioplasty. It has been suggested that probucol inhibits VSMCs proliferation, but this effect has not been directly demonstrated. In this study we investigated the effect of probucol on neointimal formation after balloon injury in normocholesterolemic rabbits and examined whether probucol could inhibit the proliferation of rabbit cultured VSMC stimulated by fetal bovine serum (FBS). Probucol inhibited the formation of neointima by about 63% 2 weeks after balloon injury. Probucol inhibited the increase in the number of cultured VSMCs and bromodeoxyuridine (BrdU) incorporation stimulated by 10% FBS in a dose-dependent manner. Also, 10% FBS stimulated the activities of mitogen-activated protein kinase (MAP kinase) and protein kinase C (PKC) in cultured VSMCs. Probucol inhibited these activities in a dose-dependent fashion. These results suggest that probucol may inhibit neointimal formation after balloon injury in normocholesterolemic rabbits by preventing the proliferation of VSMCs via inactivation of MAP kinase and PKC.


Asunto(s)
Anticolesterolemiantes/farmacología , Arterias Carótidas/efectos de los fármacos , Músculo Liso Vascular/efectos de los fármacos , Probucol/farmacología , Túnica Íntima/efectos de los fármacos , Animales , Arterias Carótidas/patología , División Celular/efectos de los fármacos , Células Cultivadas , Colesterol/sangre , Lipoproteínas HDL/sangre , Músculo Liso Vascular/metabolismo , Inhibidores de Proteínas Quinasas , Conejos , Triglicéridos/sangre
16.
Neurol Med Chir (Tokyo) ; 38 Suppl: 152-5, 1998.
Artículo en Inglés | MEDLINE | ID: mdl-10234998

RESUMEN

We studied the effects of intracarotid papaverine and prostaglandin E1 incorporated in lipid microsphere (Lipo-PGE1) in relation with transcranial Doppler parameters such as mean flow velocity (MFV) and pulsatile index (PI) of the proximal segment of the middle cerebral artery. Eighty patients with subarachnoid hemorrhage (SAH) were included in this study. In the case of angiographic vasospasm, papaverine at 7 mg/min with total dose below 300 mg per artery and 10-20 micrograms of Lipo-PGE1 were injected in the supraclinoid portion of the internal carotid artery. Vasospasm was improved in 24 patients (63%), however, it was unchanged in 14 patients (37%). The former patients had more favorable outcomes than the latter patients (p < 0.005). After intracarotid injection therapy, the correlation between MFV and PI was classified into three types: type 1, both MFV and PI decreased; type 2, MFV decreased but PI increased; and type 3, both MFV and PI fluctuated. The Glasgow Outcome Scale 3 months after SAH was as follows: type 1 (n = 15), good in 14 (93%) and moderate disability in one (7%); type 2 (n = 9), good in eight (89%) and vegetative state in one (11%); and type 3 (n = 14), moderate disability in five (36%), severe disability in seven (50%), and death in two (14%). Chi-square analysis showed significant differences between type 1 and type 3 (p < 0.005), and type 2 and type 3 (p < 0.005). In conclusion, intracarotid papaverine combined with Lipo-PGE1 was effective for vasospasm but type 3 patients require a different treatment protocol.


Asunto(s)
Alprostadil/uso terapéutico , Arterias Carótidas/diagnóstico por imagen , Ataque Isquémico Transitorio/diagnóstico por imagen , Ataque Isquémico Transitorio/tratamiento farmacológico , Papaverina/uso terapéutico , Vasodilatadores/uso terapéutico , Encéfalo/irrigación sanguínea , Humanos , Inyecciones Intraarteriales , Microesferas , Resultado del Tratamiento , Ultrasonografía Doppler Transcraneal/métodos
17.
Cardiovasc Drugs Ther ; 11(4): 567-74, 1997 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-9358961

RESUMEN

The effects of cerivastatin sodium (BAY w 6228), a new type of inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, on plasma cholesterol concentrations and the induction of hepatic LDL receptors were investigated with beagle dogs and Hep G2 cells. Oral administration of cerivastatin (0.01, 0.03, and 0.1 mg/kg per day) for 3 weeks reduced plasma total and very low-density lipoprotein plus low-density lipoprotein (VLDL + LDL) cholesterol concentrations and increased hepatic LDL receptor binding activity in dogs. Scatchard plot analysis revealed a 1.9-fold increase in the maximum binding capacity of hepatic LDL receptors in cerivastatin-treated animals. Similar results were obtained by administration of pravastatin (1.0 and 5.0 mg/kg/day) for 3 weeks. Binding activity of the LDL receptor, as well as receptor mRNA and protein concentrations, were increased in a dose-dependent manner (0.01-1.0 microM) by exposure of Hep G2 cells to cerivastatin. The results suggest that cerivastatin reduces plasma cholesterol concentrations by increasing hepatic LDL receptor expression. The mechanism of lowering cholesterol concentration by cerivastatin was the same as with the other previously examined HMG-CoA reductase inhibitors, but the effects with cerivastatin were apparent at doses much lower than the effective doses of the other drugs. Cerivastatin, therefore, shows potential for clinical use as a potent and efficacious plasma cholesterol-lowering drug.


Asunto(s)
Colesterol/sangre , Inhibidores de Hidroximetilglutaril-CoA Reductasas/farmacología , Hígado/efectos de los fármacos , Piridinas/farmacología , Receptores de LDL/metabolismo , Animales , Northern Blotting , Línea Celular , Perros , Humanos , Lipoproteínas LDL/metabolismo , Hígado/metabolismo , Masculino , Microsomas Hepáticos/efectos de los fármacos , Microsomas Hepáticos/metabolismo , ARN Mensajero/análisis , Receptores de LDL/genética
18.
DNA Res ; 4(4): 273-80, 1997 Aug 31.
Artículo en Inglés | MEDLINE | ID: mdl-9405935

RESUMEN

The extensive sequence homology that exists among the catalytic domains of phosphatidylinositol 3- and 4-kinases allowed us to clone a novel human gene encoding a putative phosphatidylinositol kinase, NPIK. Among other known phosphatidylinositol 3- and 4-kinases, NPIK was most closely related to yeast PIK1 phosphatidylinositol 4-kinase. Several forms of NPIK cDNAs were isolated, and expression of NPIK message was detected in a wide variety of tissues. Fluorescence in situ hybridization and radiation hybrid analyses assigned the NPIK gene to human chromosome 1. Recombinant NPIK protein catalyzed a conversion from phosphatidylinositol to phosphatidylinositol 4-phosphate. The catalytic activity of NPIK was augmented by Triton X-100, and was reduced in the presence of adenosine. Using green fluorescent protein system we determined that NPIK is localized in the cytoplasm. Taken together, the data suggest that NPIK may play a pivotal role in regulating the synthesis of phosphatidylinositol 4-phosphate at the site(s) accessible from cytoplasm.


Asunto(s)
1-Fosfatidilinositol 4-Quinasa/metabolismo , Proteínas de Saccharomyces cerevisiae , 1-Fosfatidilinositol 4-Quinasa/genética , Secuencia de Aminoácidos , Secuencia de Bases , Mapeo Cromosómico , Cromosomas Humanos Par 1 , Clonación Molecular , ADN Complementario , Humanos , Hibridación Fluorescente in Situ , Datos de Secuencia Molecular , ARN Mensajero/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Fracciones Subcelulares/enzimología
19.
Metabolism ; 46(4): 355-8, 1997 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-9109834

RESUMEN

We determined the most effective dosage of pentaerythritol tetranicotinate (niceritrol) to reduce plasma lipoprotein(a) [Lp(a)] levels in 44 Japanese patients (16 men and 28 women; mean age, 59.2 +/- 10.8 years) with hyperlipidemia types IIa, IIb, and IV. Patients received oral niceritrol at a dosage of 750 mg (3 tablets)/d for 8 weeks, followed by 1,500 mg (6 tablets)/d for 8 weeks. Administration of niceritrol 750 mg/d for 8 weeks decreased total and low-density lipoprotein (LDL) cholesterol in patients with type IIa hyperlipidemia and decreased triglycerides in patients with type IV hyperlipidemia, but did not affect Lp(a). However, niceritrol 1,500 mg/d for 8 weeks decreased Lp(a) in patients with initial Lp(a) levels greater than 30 mg/dL in addition to decreasing total and LDL cholesterol and triglycerides. These results suggest that the effective dosage of niceritrol to reduce the serum Lp(a) concentration in Japanese hyperlipidemic patients with a high Lp(a) level (> or = 30 mg/dL) is greater than 1,500 mg/d.


Asunto(s)
Hipolipemiantes/farmacología , Lipoproteína(a)/sangre , Niceritrol/farmacología , Anciano , HDL-Colesterol/sangre , LDL-Colesterol/sangre , Femenino , Humanos , Masculino , Persona de Mediana Edad , Triglicéridos/sangre
20.
Cytogenet Cell Genet ; 77(3-4): 261-3, 1997.
Artículo en Inglés | MEDLINE | ID: mdl-9284931

RESUMEN

A full-length cDNA homologous to RAB7, a member of the RAB-related GTP-binding protein subfamily, was isolated from a human placenta cDNA library. This cDNA, designated RAB7L1, has an open reading frame of 609 nucleotides encoding 203 amino acids. Northern analysis showed that the mRNA is ubiquitously expressed in human tissues, although signal intensities were different among the various organs examined. This gene was located on chromosome band 1q32 by fluorescence in situ hybridization.


Asunto(s)
Cromosomas Humanos Par 1/genética , Proteínas de Unión al GTP/genética , Proteínas de Unión al GTP rab , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Mapeo Cromosómico , Cromosomas Humanos Par 1/ultraestructura , Clonación Molecular , ADN Complementario/genética , Perros , Femenino , Humanos , Hibridación Fluorescente in Situ , Datos de Secuencia Molecular , Placenta/metabolismo , Embarazo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas , Homología de Secuencia de Aminoácido , Proteínas de Unión a GTP rab7
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