Spatiotemporal-Dependent Confinement Effect of Bubble Swarms Enables a Fractal Hierarchical Assembly with Promoted Chirality.

The submarine-confined bubble swarm is considered an important constraining environment for the early evolution of living matter due to the abundant gas/water interfaces it provides. Similarly, the spatiotemporal characteristics of the confinement effect in this particular scenario may also impact the origin, transfer, and amplification of chirality in organisms. Here, we explore the confinement effect on the chiral hierarchical assembly of the amphiphiles in the confined bubble array stabilized by the micropillar templates. Compared with the other confinement conditions, the assembly in the bubble scenario yields a fractal morphology and exhibits a unique level of the chiral degree, ordering, and orientation consistency, which can be attributed to the characteristic interfacial effects of the rapidly formed gas/water interfaces. Thus, molecules with a balanced amphiphilicity can be more favorable for the promotion. Not limited to the pure enantiomers, chiral amplification of the enantiomer-mixed assembly is observed only in the bubble scenario. Beyond the interfacial mechanism, the fast formation kinetics of the confined liquid bridges in the bubble scenario endows the assembly with the tunable hierarchical morphology when regulating the amphiphilicity, aggregates, and confined spaces. Furthermore, the chiral-induced spin selectivity (CISS) effect of the fractal hierarchical assembly was systematically investigated, and a strategy based on photoisomerization was developed to efficiently modulate the CISS effect. This work provides insights into the robustness of confined bubble swarms in promoting a chiral hierarchical assembly and the potential applications of the resulting chiral hierarchical patterns in solid-state spintronic and optical devices.

Staphylococcus warneri strain XSB102 exacerbates psoriasis and promotes keratinocyte proliferation in imiquimod-induced psoriasis-like dermatitis mice.

Psoriasis is one of the common chronic inflammatory skin diseases worldwide. The skin microbiota plays a role in psoriasis through regulating skin homeostasis. However, the studies on the interactions between symbiotic microbial strains and psoriasis are limited. In this study, Staphylococcus strain XSB102 was isolated from the skin of human, which was identified as Staphylococcus warneri using VITEK2 Compact. To reveal the roles of Staphylococcus warneri on psoriasis, XSB102 were applied on the back of imiquimod-induced psoriasis-like dermatitis mice. The results indicated that it exacerbated the psoriasis and significantly increased the thickening of the epidermis. Furthermore, in vitro experiments confirmed that inactivated strain XSB102 could promote the proliferation of human epidermal keratinocytes (HaCaT) cell. However, real-time quantitative PCR and immunofluorescence results suggested that the expression of inflammatory factors such as IL-17a, IL-6, and so on were not significantly increased, while extracellular matrix related factors such as Col6a3 and TGIF2 were significantly increased after XSB102 administration. This study indicates that Staphylococcus warneri XSB102 can exacerbate psoriasis and promote keratinocyte proliferation independently of inflammatory factors, which paves the way for further exploration of the relationship between skin microbiota and psoriasis.

Volatile Dual-Solvent Assisted Intermediate Phase Regulation for Anti-Solvent-Free Perovskite Photovoltaics.

The unprecedented development of perovskite solar cells (PSCs) makes them one of the most promising candidates for terawatt-scale green energy production with low cost. However, the high boiling point solvents during the solution-processed film deposition cause anisotropic crystal growth and toxic solvent vapor during high-throughput manufacturing. Here, a dual-component green solvent consisting of isopropyl acetate and acetonitrile is proposed to form a volatile perovskite precursor, which can realize the high-quality perovskite thin film deposition by intermediate phase regulation. A room-temperature stable perovskite intermediate phase is constructed with the engagement of isopropyl acetate as co-solvent, which suppresses the exploding nucleation rate in volatile perovskite precursor, providing a fine grain growth rate and wide processing window in scalable film deposition. The corresponding PSCs fabricated by blade coating without anti-solvents or gas quenching achieve power conversion efficiency (PCE) of 16.37 % and 15.29 % for the areas of 14.08 cm2 and 37.83 cm2 , respectively.

Humidity-Controlled Molecular Assembly and Photoisomerization Behavior with a Bubble-Assisted Patterning Approach.

Precise control of molecular assembly is of great significance in the application of functional molecules. This work has systematically investigated the humidity effect in bubble-assisted molecular assembly. This work finds humidity is critical in the evolution of the soft confined space, leading to the formation of microscale liquid confined space under high humidity, and nanoscale liquid confined space under low humidity. It is also revealed that the differences in surface wettability and adhesion play the key role. Consequently, a flat pattern with thermodynamically favorable ordered structure and a sharp pattern with dynamically favorable disordered structure are achieved, which show different solid-state photoisomerization behaviors and photoresponsiveness. Interestingly, conductivity of sharp pattern with disordered structure is higher than that of flat pattern with layered ordered structure due to electronic transport mechanism of different spatial dimensions. This work opens a new way for manipulating the molecular self-assembly to control the morphology and function of molecular patterns.

Bubble wall confinement-driven molecular assembly toward sub-12 nm and beyond precision patterning.

Patterning is attractive for nanofabrication, electron devices, and bioengineering. However, achieving the molecular-scale patterns to meet the demands of these fields is challenging. Here, we propose a bubble-template molecular printing concept by introducing the ultrathin liquid film of bubble walls to confine the self-assembly of molecules and achieve ultrahigh-precision assembly up to 12 nanometers corresponding to the critical point toward the Newton black film limit. The disjoining pressure describing the intermolecular interaction could predict the highest precision effectively. The symmetric molecules exhibit better reconfiguration capacity and smaller preaggregates than the asymmetric ones, which are helpful in stabilizing the drainage of foam films and construct high-precision patterns. Our results confirm the robustness of the bubble template to prepare molecular-scale patterns, verify the criticality of molecular symmetry to obtain the ultimate precision, and predict the application potential of high-precision organic patterns in hierarchical self-assembly and high-sensitivity sensors.

Orientation-Controlled Ultralong Assembly of Janus Particles Induced by Bubble-Driven Instant Quasi-1D Interfaces.

Constructing precisely oriented assemblies and exploring their orientation-dependent properties remain a challenge for Janus nanoparticles (JNPs) due to their asymmetric characteristics. Herein, we propose a bubble-driven instant quasi-1D interfacial strategy for the oriented assembly of JNP chains in a highly controllable manner. It is found that the rapid formation of templated bubbles can promote the interfacial orientation of JNPs kinetically, while the confined quasi-1D interface in the curved liquid bridge can constrain the disordered rotation of the particles, yielding well-oriented JNP chains in a long range. During the evaporation process, the interfacial orientation of the JNPs can be transferred to the assembled chains. By regulating the amphiphilicity of the JNPs, both heteraxial and coaxial JNP assemblies are obtained, which show different polarization dependences on light scattering, and the related colorimetric logic behaviors are demonstrated. This work demonstrates the great potential of patterned interfacial assembly with a manageable orientation and shows the broad prospect of asymmetric JNP assembly in constructing novel optoelectronic devices.

Passive silicon on-chip optic accelerometer for low frequency vibration detection.

In this article, a fiber optic accelerometer based on a Fabry-Perot interferometer is presented and prepared by micromachining on a silicon substrate. The inertial sensing structure of the accelerometer is built by a center block mass and four folded springs with a high thickness-to-width ratio, providing an in-plane resonance vibration. The miniature-sized sensor has large flexibility in structural design and its acceleration response can be predicted by theoretical estimation and finite element simulation. Experimental results show that the proposed sensor's sensitivity is over 161 rad/g in frequency range of 1 to 63 Hz. It also achieved a low average noise level of 67.4 ng/Hz1/2 on the working band. The sensor's transverse crosstalk is less than 1.38%, proving its potential ability in low frequency vibration detection applications.

Miniature tri-axis accelerometer based on fiber optic Fabry-Pérot interferometer.

A fiber optic accelerometer with a high sensitivity, low noise, and compact size is proposed for low-frequency acceleration sensing. The sensor is composed of a 20 mm diameter spherical outer frame and a three-dimensional spring-mass structure as the inertial sensing element. Three Fabry-Pérot interferometers (FPI) are formed between flat fiber facets and cubic mass surfaces to measure the FPI cavity length change caused by acceleration. The dynamic signal sensing of the designed accelerometer is performed, which shows a high acceleration sensitivity of 42.6 dB re rad/g with a working band of 1-80 Hz. An average minimum detectable acceleration of 4.5 µg/Hz1/2 can be obtained. The sensor features simple assembling, small size, light weight, and good consistency. Its transverse sensitivity is measured to be less than 3% (-30 dB) of the sensitive axis. The experimental result indicates that the proposed accelerometer has application potential in areas such as seismic wave detection and structural health monitoring.

3D Optical Heterostructure Patterning by Spatially Allocating Nanoblocks on a Printed Matrix.

Heterostructures have attracted enormous interest due to the properties arising from the coupling and synergizing between multiscale structures and the promising applications in electronics, mechanics, and optics. However, it is challenging for current technologies to precisely integrate cross-scale micro/nanomaterials in three dimensions (3D). Herein, we realize the precise spatial allocation of nanoblocks on micromatrices and programmable 3D optical heterostructure patterning via printing-assisted self-assembly. This bottom-up approach fully exploits the advantages of printing in on-demand patterning, low cost, and mass production, as well as the merits of solution-based colloidal assembly for simple structuring and high-precision regulating, which facilitates the patterned integration of multiscale materials. Importantly, the luminescent nanoparticle assembly can be accurately coupled to the dye-doped polymer matrix by regulating the interface wettability, enabling facile multicolor tuning in a single heterostructure. Thus, the heterostructure can be specially encoded for anticounterfeiting and encryption applications due to the morphology-dependent and interface-coupling-induced luminescence. Moreover, with the capability to achieve single-nanoparticle resolution, these findings have great potential for designing photonic superstructures and advanced optical devices.

Influence of Surfacing Fe-Based Alloy Layers on Wire Arc Additive Manufactured Ni-Based Superalloys Material on Its Microstructure and Wear Properties.

Wire arc additively manufactured (WAAM) Ni-based materials have good properties but are costly and hard to cut, leading to difficulties in machining after welding and wasting the materials. To overcome these shortcomings, this work proposes a method of surfacing Fe-based alloy layers on WAAM Ni-based material. The effect of this method on the microstructure and wear properties of WAAM Ni-based materials is discussed. In this work, a Fe-based alloy (JX103) was welded as the last layers of the WAAM Ni-based superalloy (JX201) material. The hardness, microstructure, and wear behavior of the material with different residual Fe-based materials were tested and analyzed. Our results indicate that the surface hardness was smoothly increased from HV350 to HV400 by overlaying Fe-based alloy layers. Microstructure analysis shows that γ-Fe gradually disappears, and the carbide form changes from WAAM Ni-based superalloys to Fe-based alloys. In the fusion boundary, the occurrence of cellular dendritic growth, a type -Ⅱ boundary, and low dilution indicate good crack resistance and good connection performance between these two materials. The wear test showed that the wear resistance of JX201 was decreased by changing the last layer to JX103. However, as the residual thickness of JX103 decreased, the influence gradually reduced. Meanwhile, the wear mechanism changed from severe abrasive and adhesive wear to light abrasive wear. When the thickness is less than 0.5 mm, the wear weight per minute is at the same level as the sample without JX103.

A Eurasian avian-like H1N1 swine influenza reassortant virus became pathogenic and highly transmissible due to mutations in its PA gene.

Previous studies have shown that the Eurasian avian-like H1N1 (EA H1N1) swine influenza viruses circulated widely in pigs around the world and formed multiple genotypes by acquiring non-hemagglutinin and neuraminidase segments derived from other swine influenza viruses. Swine influenza control is not a priority for the pig industry in many countries, and it is worrisome that some strains may become more pathogenic and/or transmissible during their circulation in nature. Our routine surveillance indicated that the EA H1N1 viruses obtained different internal genes from different swine influenza viruses and formed various new genotypes. In this study, we found that a naturally isolated swine influenza reassortant, A/swine/Liaoning/265/2017 (LN265), a representative strain of one of the predominant genotypes in recent years, is lethal in mice and transmissible in ferrets. LN265 contains the hemagglutinin, neuraminidase, and matrix of the EA H1N1 virus; the basic polymerase 2, basic polymerase 1, acidic polymerase (PA), and nucleoprotein of the 2009 H1N1 pandemic virus; and the nonstructural protein of the North American triple-reassortment H1N2 virus. By generating and testing a series of reassortants and mutants, we found that four gradually accumulated mutations in PA are responsible for the increased pathogenicity and transmissibility of LN265. We further revealed that these mutations increase the messenger RNA transcription of viral proteins by enhancing the endonuclease cleavage activity and viral RNA-binding ability of the PA protein. Our study demonstrates that EA H1N1 swine influenza virus became pathogenic and transmissible in ferrets by acquiring key mutations in PA and provides important insights for monitoring field strains with pandemic potential.

Four-wavelength quadrature phase demodulation technique for extrinsic Fabry-Perot interferometric sensors.

In this Letter, we report a four-wavelength quadrature phase demodulation technique for extrinsic Fabry-Perot interferometric (EFPI) sensors and dynamic signals. Four interferometric signals are obtained from four different laser wavelengths. A wavelength interval of four wavelengths is chosen according to the free spectrum range (FSR) of EFPI sensors to generate two groups of anti-phase signals and two groups of orthogonal signals. The linear fitting (LF) method is applied to two groups of anti-phase signals to eliminate the dc component and ac amplitude to obtain two normalized orthogonal signals. The differential cross multiplication (DCM) method is then used to demodulate the phase signal from these two normalized orthogonal signals. The proposed LF and DCM (LF-DCM) based four-wavelength quadrature phase demodulation overcomes the drawback of the traditional ellipse fitting (EF) and DCM (EF-DCM) based dual-wavelength demodulation method that it is not suitable for weak signal demodulation since the ellipse degenerates into a straight line, which makes the EF algorithm invalid. Moreover, it also avoids the assumption that the dc component and ac amplitude of interferometric signals are identical, which is widely used in three-wavelength demodulation. An EFPI acoustic sensor is tested to prove the four-wavelength quadrature phase demodulation and experimental results show that the proposed phase demodulation method shows advantages of large dynamic range and wide frequency band. Linearity is as high as 0.9999 and a high signal-to-noise ratio (SNR) is observed from 1 Hz to 100 kHz.

Large-Dynamic-Range and High-Stability Phase Demodulation Technology for Fiber-Optic Michelson Interferometric Sensors.

A large-dynamic-range and high-stability phase demodulation technology for fiber-optic Michelson interferometric sensors is proposed. This technology utilizes two output signals from a 2 × 2 fiber-optic coupler, the interferometric phase difference of which is π. A linear-fitting trigonometric-identity-transformation differential cross-multiplication (LF-TIT-DCM) algorithm is used to interrogate the phase signal from the two output signals from the coupler. The interferometric phase differences from the two output signals from the 2 × 2 fiber-optic couplers with different coupling ratios are all equal to π, which ensures that the LF-TIT-DCM algorithm can be applied perfectly. A fiber-optic Michelson interferometric acoustic sensor is fabricated, and an acoustic signal testing system is built to prove the proposed phase demodulation technology. Experimental results show that excellent linearity is observed from 0.033 rad to 3.2 rad. Moreover, the influence of laser wavelength and optical power is researched, and variation below 0.47 dB is observed at different sound pressure levels (SPLs). Long-term stability over thirty minutes is tested, and fluctuation is less than 0.36 dB. The proposed phase demodulation technology obtains large dynamic range and high stability at rather low cost.

A Direct Writing Approach for Organic Semiconductor Single-Crystal Patterns with Unique Orientation.

Organic semiconductor single-crystal (OSSC) patterns with precisely controlled orientation are of great significance to the integrated fabrication of devices with high and uniform performance. However, it is still challenging to achieve purely oriented OSSC patterns due to the complex nucleation and growth process of OSSCs. Here, a general direct writing approach is presented to readily obtain high-quality OSSC patterns with unique orientation. In specific, a direct writing method is demonstrated wherein the microscale meniscus is manipulated, which makes it possible to precisely control the nucleation and growth process of the OSSC because of its comparable size to the crystal nuclei. The resulting OSSC patterns are highly crystalline and purely oriented, in which each ribbon crystal shows a deviation angle of 33° to the printing direction. The mechanism of orientation purification is revealed experimentally and theoretically, and the results show that the TCL deformation caused by the difference in wettability and adhesive force, as well as the asymmetry of fluid concentration distribution, are the key factors leading to the selective deposition and unique orientation. Moreover, organic field-effect transistors (OFETs) and polarization-sensitive photodetectors are prepared based on the OSSC patterns with unique orientation, which exhibit higher device performance compared to the non-purely oriented crystal-based OFETs.

Sensitivity enhanced fiber optic hydrophone based on an extrinsic Fabry-Perot interferometer for low-frequency underwater acoustic sensing.

A miniaturized fiber optic hydrophone (FOH) based on a composite metal diaphragm with an air back cavity and a high finesse extrinsic Fabry-Perot interferometric (EFPI) scheme for low-frequency underwater acoustic sensing is proposed and experimentally demonstrated in this paper. A composite metal diaphragm is used to improve the stability of the hydrophone. A balance channel is used to equilibrate the hydrostatic pressure and maintain an air cavity, which improves the mechanical sensitivity. In addition, a white light interferometry (WLI) phase demodulation is used to demodulate the high finesse interferometer consisted of the fiber collimator end face and the diaphragm, which improves the phase sensitivity. Experimental results show that the enhanced phase sensitivity of the hydrophone is about -122.5 dB re 1 rad/µPa @ 200 Hz and the sensitivity fluctuation is below 2.5 dB between 3 Hz and 400 Hz, while the minimal detectable pressure (MDP) is 63.7 µPa/Hz1/2 @ 400 Hz. Due to its miniaturized structure and high sensitivity, the FOH may have an enormous potential in underwater target detection.

Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China.

The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls; the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza.

Computational Insights Into the Effects of the R190K and N121Q Mutations on the SARS-CoV-2 Spike Complex With Biliverdin.

The SARS-CoV-2 spike has been regarded as the main target of antibody design against COVID-19. Two single-site mutations, R190K and N121Q, were deemed to weaken the binding affinity of biliverdin although the underlying molecular mechanism is still unknown. Meanwhile, the effect of the two mutations on the conformational changes of "lip" and "gate" loops was also elusive. Thus, molecular dynamics simulation and molecular mechanics/generalized Born surface area (MM/GBSA) free energy calculation were conducted on the wild-type and two other SARS-CoV-2 spike mutants. Our simulations indicated that the R190K mutation causes Lys190 to form six hydrogen bonds, guided by Asn99 and Ile101, which brings Lys190 closer to Arg102 and Asn121, thereby weakening the interaction energy between biliverdin and Ile101 as well as Lys190. For the N121Q mutation, Gln121 still maintained a hydrogen bond with biliverdin; nevertheless, the overall binding mode deviated significantly under the reversal of the side chain of Phe175. Moreover, the two mutants would stabilize the lip loop, which would restrain the meaningful upward movement of the lip. In addition, N121Q significantly promoted the gate loop deviating to the biliverdin binding site and compressed the site. This work would be useful in understanding the dynamics binding biliverdin to the SARS-CoV-2 spike.

Passive Homodyne Phase Demodulation Technique Based on LF-TIT-DCM Algorithm for Interferometric Sensors.

A passive homodyne phase demodulation technique based on a linear-fitting trigonometric-identity-transformation differential cross-multiplication (LF-TIT-DCM) algorithm is proposed. This technique relies on two interferometric signals whose interferometric phase difference is odd times of π. It is able to demodulate phase signals with a large dynamic range and wide frequency band. An anti-phase dual wavelength demodulation system is built to prove the LF-TIT-DCM algorithm. Comparing the traditional quadrature dual wavelength demodulation system with an ellipse fitting DCM (EF-DCM) algorithm, the phase difference of two interferometric signals of the anti-phase dual wavelength demodulation system is set to be π instead of π/2. This technique overcomes the drawback of EF-DCM-that it is not able to demodulate small signals since the ellipse degenerates into a straight line and the ellipse fitting algorithm is invalidated. Experimental results show that the dynamic range of the proposed anti-phase dual wavelength demodulation system is much larger than that of the traditional quadrature dual wavelength demodulation system. Moreover, the proposed anti-phase dual wavelength demodulation system is hardly influenced by optical power, and the laser wavelength should be strictly limited to lower the reference error.

A Novel Intronic Circular RNA Antagonizes Influenza Virus by Absorbing a microRNA That Degrades CREBBP and Accelerating IFN-ß Production.

Virus-host interactions are complicated processes, and multiple cellular proteins promote or inhibit viral replication through different mechanisms. Recent progress has implicated circular RNAs (circRNAs) in cancer biology and progression; however, the role of circRNAs in viral infection remains largely unclear. Here, we detected 11,620 circRNAs in A549 cells and found that 411 of them were differentially expressed in influenza virus-infected A549 cells. We characterized a novel intronic circRNA, AIVR, that was upregulated in influenza virus-infected A549 cells and found that silencing of AIVR significantly promoted influenza virus replication in A549 cells. We further found that AIVR predominantly localizes in the cytoplasm and works as a microRNA (miRNA) sponge. One of the miRNAs absorbed by AIVR binds the mRNA of CREBBP, which is an important component of the large nucleoprotein complex interferon beta (IFN-ß) enhanceosome that accelerates IFN-ß production. AIVR overexpression significantly increased the mRNA and protein levels of IFN-ß in the influenza virus-infected A549 cells. Therefore, the upregulation of AIVR is a cellular antiviral strategy, with AIVR exerting its antiviral effect by absorbing miRNA and promoting the expression of CREBBP to facilitate IFN-ß production. Our study provides new insights into the roles of circRNAs in the cellular innate antiviral response. IMPORTANCE Circular RNAs (circRNAs) are new members of the long noncoding RNA families and have been identified in a variety of organisms, including plants, animals, and humans. Accumulating data indicate that circRNAs perform multiple functions in a variety of cellular processes associated with human diseases, such as Alzheimer's disease and cancer; however, the roles of circRNAs in virus infection have been largely uninvestigated. In this study, we investigated the cellular circRNA response upon influenza virus infection and found that 411 circRNAs were differentially expressed in the virus-infected cells. We identified a novel human intronic circRNA (we named AIVR) that antagonizes influenza virus replication. Upregulated circRNA AIVR absorbs an miRNA that binds the mRNA of CREBBP, leading to an increase in the cellular expression of CREBBP and then accelerating IFN-ß production. This study advances the understanding of the roles of circRNAs in the cellular innate antiviral response.

A Bubble-Assisted Approach for Patterning Nanoscale Molecular Aggregates.

We demonstrate a new approach to pattern functional organic molecules with a template of foams, and achieve a resolution of sub 100 nm. The bubble-assisted assembly (BAA) process is consisted of two periods, including bubble evolution and molecular assembly, which are dominated by the Laplace pressure and molecular interactions, respectively. Using TPPS (meso-tetra(4-sulfonatophenyl) porphyrin), we systematically investigate the patterns and assembly behaviour in the bubble system with a series of characterizations, which show good uniformity in nanoscale resolution. Theoretical simulations reveal that TPPS's J-aggregates contribute to the ordered construction of molecular patterns. Finally, we propose an empirical rule for molecular patterning approach, that the surfactant and functional molecules should have the same type of charge in a two-component system. This approach exhibits promising feasibility to assemble molecular patterns at nanoscale resolution for micro/nano functional devices.