Assessment of free-radical scavenging activity of Gypsophila pilulifera: assay-guided isolation of verbascoside as the main active component

Gypsophila pilulifera, Boiss & Heldr, Caryophyllaceae, is a perennial medicinal herb that grows in the southwestern region of Turkey. Except for only one report on the isolation of cytotoxic saponins from the underground parts of G. pilulifera, there are no published thorough phytochemical or bioactivity studies on this species. In the present study, the free-radical scavenging activity of extracts and fractions of the stems of G. pilulifera was evaluated, using a slightly modified and more precise version of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, reported here for the first time. The DPPH assay-guided HPLC-PDA-purification of the active solid-phase extraction fraction (50% methanol in water) of the methanolic extract exhibited verbascoside as the main free-radical scavenger present in this species. The structure of this active compound was resolved by spectroscopy, and the free-radical scavenging potential of verbascoside was determined.

Phytochemistry and bioactivity of Pedicularis sibthorpii growing in Iran

The methanol extract of the aerial parts of the medicinal plant Pedicularis sibthorpii Boiss., Scrophulariaceae, growing in the Azerbaijan province of Iran, was found to be active in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and the antibacterial agar well diffusion assays, but no general toxicity was observed in the brine shrimp lethality assay. A combination of solid-phase extraction (SPE) and preparative reversed-phase high-performance liquid chromatography (prep-RP-HPLC) analyses of the methanolic extract afforded three phenylethanoids (verbascoside, martynoside and isomartynoside), an iridoid (aucubin), a flavonoid (luteolin 7-O-β-D-glucopyranoside) and mannitol, and the structures of these compounds were elucidated unambiguously by spectroscopic means. The distribution of the isolated compounds within the genus Pedicularis has also been discussed.

Essential oil composition and isolation of freeradical-scavenging phenolic glycosides from the aerial parts of Ajuga chamaepitys growing in Iran

From the methanolic extract of the aerial parts of Ajuga chamaepitys (L.) Schreb., Lamiaceae, one of the Iranian medicinal plants, the phenylethanoid glycoside, acteoside, and two flavone glycosides, chrysoeriol 7-O-glucopyranoside (3'-methoxy-luteolin 7-O-glucopyranoside) and apigenin 7-O-rhamnopyranoside, were isolated by a combination of solid-phase extraction (SPE) and preparative reversed-phase high-performance liquid chromatography (prep-RP-HPLC) methods. Structures of the isolated compounds were elucidated by spectroscopic means. The free-radical-scavenging properties of the extracts, fractions and isolated compounds were determined by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. While among the extracts, the MeOH extract showed the highest level of free-radical-scavenging activity (RC50 1.15 × 10-1 mg/mL), chrysoeriol 7-O-glucopyranoside was the most active (RC50 3.00 × 10-3 mg/mL) among the isolated compounds. The GC-MS and the GC-FID analyses revealed α-pinene (23.66%), β-pinene (9.33%), 1-octen-3-ol (9.72%), β-phellandrene (8.70%) and germacrene-D (7.92%) as the major components of the essential oils derived from the aerial parts of this plant. The presence of phenolic glycosides and the α- and β-pinene-rich essential oils in A. chamaepitys may provide some rationale for the traditional medicinal uses of this species in Iran.

Evaluation of antimalarial, free-radical-scavenging and insecticidal activities of Artemisia scoparia and A. Spicigera, Asteraceae

Artemisia species (Asteraceae), widespread throughout the world, are a group of important medicinal plants. The extracts of two medicinal plants of this genus, Artemisia scoparia Waldst. & Kit. and A. spicigera C. Koch, were evaluated for potential antimalarial, free-radical-scavenging and insecticidal properties, using the heme biocrystallisation and inhibition assay, the DPPH assay and the contact toxicity bioassay using the pest Tribolium castaneum, respectively. The methanol extracts of both species showed strong free-radical-scavenging activity and the RC50 values were 0.0317 and 0.0458 mg/mL, respectively, for A. scoparia and A. spicigera. The dichloromethane extracts of both species displayed a moderate level of potential antimalarial activity providing IC50 at 0.778 and 0.999 mg/mL for A. scoparia and A. spicigera, respectively. Both species of Artemisia showed insecticidal properties. However, A. spicigera was more effective than A. scoparia.

Composition, antioxidant and chemotherapeutic properties of the essential oils from two Origanum species growing in Pakistan

The GC-MS analyses of Origanum majorana L. (OME) and Origanum vulgare L. (OVE), Lamiaceae, essential oils helped identification of 39 (96.4 percent of the total oils) and 43 (92.9 percent of the total oils) components, respectively. The major constituents of OME were terpinene-4-ol (20.9 percent), linalool (15.7 percent), linalyl-acetate (13.9 percent), limonene (13.4 percent) and α-terpineol (8.57 percent), whereas, thymol (21.6 percent), carvacrol (18.8 percent), o-cymene (13.5 percent) and α-terpineol (8.57 percent) were the main components of OVE. In the disc diffusion and the resazurin microtitre assays, OME showed better antibacterial activity than OVE with larger zones of inhibition (16.5-27.0 mm) and smaller MIC (40.9-1250.3 μg/mL) against the tested bacterial strains. Only OVE displayed anti-heme biocrystallization activity with an IC50 at 0.04 mg/mL. In the DPPH assay, OVE showed better radical-scavenging activity than OME (IC50=65.5 versus 89.2 μg/mL) and both OME and OVE inhibited lionleic acid oxidation. However, in the bleaching β-carotene assay, OVE exhibited better antioxidant activity than OME. In the MTT assay, OME was more cytotoxic than OVE against different cancer cell types, such as MCF-7, LNCaP and NIH-3T3, with IC50s of 70.0, 85.3 and 300.5 μg/mL, respectively. Overall, some components of OME and OVE may have antiparasitic and chemotherapeutic activity.

Anthocephalus cadamba (Roxb.) Miq., Rubiaceae, extract shows hypoglycemic effect and eases oxidative stress in alloxan-induced diabetic rats

The hydroethanolic extract of the flowering tops of Anthocephalus cadamba (Roxb.) Miq., Rubiaceae, a Bangladeshi medicinal plant, was studied for its potential hypoglycemic effect and antioxidant property in alloxan-induced diabetic rats. The extract induced significant reduction in serum glucose, and transaminases, e.g. aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatases (ALP), activities. Significant changes in the thiobarbituric acid reactive substances (TBARS), peroxidase and catalase levels during the experimental period were also observed. The results established that the hydroethanolic extract of the flowering tops of A. cadamba possesses hypoglycemic property and is able to protect liver and brain from oxidative damages caused by diabetes.

Anti-inflammatory activity of Adenanthera pavonina L., Fabaceae, in experimental animals / Atividade anti-inflamatória de Adenanthera pavonina L., Fabaceae, em animais experimentais

Adenanthera pavonina L, Fabaceae alt. Leguminosae, Bengali name 'rakta kombol', is an Indian medicinal plant. It is endemic to Southern China and India, and widely naturalized in Malaysia, Western and Eastern Africa as well as in most islands of both the Pacific and Caribbean regions. This plant has been used in traditional medicine for the treatment of asthma, boil, diarrhoea, gout, inflammations, rheumatism, tumour and ulcers, and as a tonic. The dried and ground bark of A. pavonina L. was extracted, successively, with petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EtOAc) and methanol (MeOH), and the resulting extracts were assessed in vivo for anti-inflammatory activity on carrageenan-induced rat hind paw oedema as a model of inflammation. The extracts were administered orally at the doses of 200 and 400 mg/kg body weight, and statistically significant (p<0.001) anti-inflammatory effects were observed in a dose dependant manner. The MeOH extract (400 mg/kg) showed 37.10 percent (p<0.01) inhibition of inflammation at the first hour of the study and, the DCM extract (400 mg/kg) exhibited 33.11 percent (p<0.001) inhibition of inflammation at the third hour of the study which was comparable with that of reference standard drug dichlofenac sodium. The results of this study supported some of the traditional medicinal uses of this plant.

Adenanthera pavonina L, Fabaceae, conhecida na região de Bengala por "rakta kombol", é uma planta medicinal endêmica do sul da China e da Índia e amplamente difundida na Malásia, África Ocidental e Oriental, bem como na maioria das ilhas de ambas as regiões do Pacífico e Caribe. Esta planta tem sido utilizada na medicina tradicional para o tratamento da asma, febre, diarréia, gota, inflamações, reumatismo tumor, úlceras, e como tônico. Cascas secas e moídas de A. pavonina foi submetida a extração sucessiva com éter de petróleo (PE), diclorometano (DCM), acetato de etila (AcOEt) e metanol (MeOH), e os extratos foram avaliados in vivo para atividade anti-inflamatória induzida por carragenina tendo como ensaio modelo de inflamação edema de pata em ratos.. Os extratos foram administrados por via oral em doses de 200 e 400 mg/kg de peso corporal, e foram observadas efeitos anti-inflamatórios de maneira dose-dependente, estatisticamente significativos (p<0,001). O extrato metanólico (400 mg/kg) apresentou 37,10 por cento (p<0,01) de inibição da inflamação na primeira hora do estudo e, o extrato DCM (400 mg/kg) apresentou 33,11 por cento (p<0,001) na inibição da inflamação na terceira hora do estudo, que foi comparável ao resultado da substância de referência diclofenato de sódio. Os resultados deste estudo confirmaram alguns dos usos tradicionais desta planta medicinal.

Free radical scavengers from the aerial parts of Grammosciadium platycarpum Boiss. & Hausskn. (Apiaceae) and GC-MS analysis of the essential oils from its fruits / Substâncias eliminadoras de radicais livres das partes aéreas de Grammosciadium platycarpum Boiss. & Hausskn. (Apiaceae) e análise por CG-MS dos óleos essenciais de seus frutos

Grammosciadium platycarpum Boiss. & Hausskn (Apiaceae) is one of three endemic Iranian species of the genus Grammosciadium DC. Consumption of the aerial parts of this plant affects renal function and causes diuresis. In the DPPH assay the methanol extract showed the highest level of free radical scavenging activity (RC50 = 1.196 x 10-2 mg/mL) among the extracts. Reversed-phase preparative HPLC analyses of the methanol extract yielded nine flavonoids, which were responsible for the free radical scavenging activity of the MeOH extract. The GC-MS analyses of the essential oils led to the identification of 29 terpenoids, mainly monoterpenes (non-oxygenated 3.97 percent and oxygenated 77.49 percent) accounting for over 96 percent of the total oils.

Grammosciadium platycarpum Boiss. & Hausskn (Apiaceae) é uma das três espécies endémicas no Irã do gênero Grammosciadium DC. Consumo da parte aérea da planta afeta a função renal e causa diurese. No ensaio por DPPH o extrato metanólico apresentou o maior nível de atividade de antioxidante por radicais livres (RC50 = 1,196 x 10-2 mg/mL) entre os extratos. Análises por HPLC preparativa de fase reversa do extrato metanólico resultou no isolamento de nove flavonóides, os quais foram responsáveis pela atividade de antioxidante do extrato metanólico. A análises por CG-EM dos óleos essenciais levou à identificação de 29 terpenóides, principalmente monoterpenos (não-oxigenados 3,97 por cento e oxigenados, 77,49 por cento), os quais representam mais de 96 por cento do total de óleos.

Antinociceptive, anti-inflammatory and diuretic properties of Polygonum barbatum (L.) Hara var. barbata / Propriedades antinociceptiva, antiinflamatória e diurética dos extratos de P. barbatum (L.) Hara var. barbata

The antinociceptive, anti-inflammatory and diuretic properties of the extracts of P. barbatum (L.) Hara var. barbata, Polygonaceae, at the doses of 200 and 400 mg/kg body weight, were evaluated in mice/rat models using, respectively, the acetic-acid-induced writhing method, the carrageenan-induced edema test and the Lipschitz method. In the acetic-acid-induced writhing test in mice, all extracts displayed a dose dependent analgesic effect. The most potent analgesic activity was observed with the petroleum ether extract at the dose of 400 mg/kg body weight with an inhibition of writhing response 46.8 percent compared to 62.2 percent for the positive control aminopyrine. Petroleum ether extract at the dose of 400 mg/kg body weight also displayed the highest levels of anti-inflammatory activity after 2 h with the 39.3 percent inhibition of paw edema, and this effect was better than the effect observed by the conventional anti-inflammatory agent phenylbutazone (maximum inhibition of 38.3 percent after 4 h). All extracts increased urine volume in a dose-dependent manner, and the ethyl acetate extract showed a significant level of diuresis comparable to that of the standard diuretic agent furosemide.

As propriedades antinociceptiva, antiinflamatória e diurética dos extratos de P. barbatum (L.) Hara var. barbata, Polygonaceae, nas doses de 200 e 400 mg/kg de peso corpóreo foram avaliadas em modelos utilizando camundongos/ratos, respectivamente, o método de contorções induzidas por ácido acético, o teste de edema induzido por carragenina e o método de Lipschitz. No método de contorções induzidas por ácido acetic, todos os extratos apresentaram efeito nociceptivo dose dependente. O efeito nociceptivo mais potente foi observado com o extrato de éter de petróleo na dose de 400 mg/kg com uma inibição das contorções de 46,8 por cento comparado com o controle positivo de aminopirina de 62,2 por cento. O extrato de éter de petróleo na dose de 400 mg/kg também mostrou maior atividade antiinflammatoria após 2 h com 39,3 por cento de inibição do edema de pata, e este efeito foi maior que o observado para o agente antiinflamatório convencional fenilbutazona (inibição máxima de 38,4 por cento após 4 h). Todos os extratos aumentaram o volume de urina de maneira dose dependente e o extrato acetato de etila mostrou um nível de significância de diurese comparável ao agente diurético padrão, furosemida.

Antidiarrhoeal property of the hydroethanolic extract of the flowering tops of Anthocephalus cadamba / Propriedade antidiarréica do extrato hidroetanólico dos topos floridos de Anthocephalus cadamba

A propriedade antidiarréica do extrato hidroetanólico dos topos floridos de Anthocephalus cadamba foi avaliada em animais experimentais. O extrato hidroetanólico seco (250-500 mg/kg massa corpórea, v.o.) exibiu uma diminuição dose-dependente do número total de excrementos na diarréia induzida por óleo de castor em camundongos. O extrato também causou uma redução significativa (p < 0.01) e dose-dependente do acúmulo de fluidos intestinais e do trânsito gastrointestinal de 64,59 por cento e 71,19 por cento nas doses de 250 e 500 mg/kg. As taxas de redução foram de 37,85 por cento e 74,91 por cento, respectivamente, com o grupo controle e da droga padrão.

The antidiarrhoeal property of the hydroethanolic extract of the flowering tops of Anthocephalus cadamba was assessed on experimental animals. The dry hydroethanolic extract (250-500 mg/kg body mass, p.o.) exhibited a dose-dependent decrease in the total number of faecal droppings in castor oil-induced diarrhoea in mice. The extract also produced a significant (p < 0.01) and dose-dependent reduction in intestinal fluids accumulation and in the gastrointestinal transit from 64.59 percent and 71.19 percent at doses of 250 and 500 mg/kg. The reduction rates were 37.85 percent and 74.91 percent, respectively, with the control and standard drug group.

Coumarins from the aerial parts of Prangos uloptera (Apiaceae) / Cumarinas das partes aéreas de Prangos uloptera (Apiaceae)

Phytochemical studies on the aerial parts of Prangos uloptera, an endemic Iranian species of the genus Prangos, yielded five coumarins, xanthotoxin (1), prangenin (2), scopoletin (3), deltoin (4) and prangolarin (5). The structures of these coumarins were elucidated by spectroscopic means, and the antioxidant potential of 1-5 was evaluated by the DPPH assay. The chemotaxonomic significance of 1-5 is also discussed.

Os estudos fitoquímicos das partes aéreas de Prangos uloptera, uma espécie do gênero Prangos endêmica do Irã, forneceram cinco cumarinas, xantotoxina (1), prangenina (2), escopoletina (3), deltoína (4) e prangolarina (5). As estruturas destas cumarinas foram elucidas através de métodos espectroscópicos e o potencial antioxidante de 1-5 foi avaliado pelo ensaio de DPPH. O significado quimiotaxonômico de 1-5 também é discutido.

Assessment of anti-hyperlipidemic effect of Citrullus colocynthis

Hyperlipidemia is a well-known risk factor for several illnesses including atherosclerosis, heart and vascular diseases and stroke. In the search for potential anti-hyperlipidemic agents from plants to prevent these conditions, the pulp and the seeds of Citrullus colocynthis were assessed for their effects on the lipid profile of hyperlipidemic New Zealand rabbits. In the experimental groups that received the pulp of C. colocynthis or 100 mg/kg of seeds, the lipid profiles were significantly reduced when compared to the control group (P<0.05).

Hiperlipidemia é um fator de risco bem conhecido para diversas doenças inclusive aterosclerose, doenças cardíacas e derrame cerebral. Na procura de potenciais agentes antihiperlipidêmicos a partir de plantas para prevenir essas doenças, a polpa e as sementes de Citrullus colocynthis foram testadas para verificar seus efeitos no perfil lipídico de coelhos Nova Zelândia hiperlipidêmicos. Nos grupos experimentais que receberam a polpa de C. colocynthis ou 100 mg/kg das sementes, os perfis lipídicos foram significantemente reduzidos quando comparados ao grupo de controle (P<0,05).

Bioactivity of two Turkish endemic Centaurea species, and their major constituents

The antioxidant activity, general toxicity and cytotoxicity of the methanol extracts of Centaurea urvillei subs. armata and C. mucronifera have been assessed, respectively, by the DPPH assay, the brine shrimp lethality and the MTT cytotoxicity assays. The reversed-phase HPLC analysis of the methanol extracts afforded two bioactive dibenzylbutyrolactone-type lignans, matairesinoside (1) and arctiin (2). The structures of these lignans were elucidated by comprehensive spectroscopic analyses as well as by the direct comparison of experimental data with respective literature data.

A atividade antioxidante, a toxicidade geral e a citotoxidade dos extratos metanólicos de Centaurea urvillei subs. armata e C. mucronifera foram analisados, respectivamente, pelo ensaio DPPH e pelos ensaios de letalidade de Artemia salina e de citotoxidade MTT. A análise dos extratos metanólicos em CLAE de fase reversa apresentou duas lignanas biotivas do tipo dibenzilbutirolactona, matairesinosídeo (1) e arctiina (2). As estruturas destas lignanas foram elucidadas através de análises espectroscópicas completas bem como por comparação direta dos dados experimentais com os respectivos dados da literatura.

Neuropharmacological properties of Launaea resedifolia

Launaea resedifolia (L.) Kuntze (family: Asteraceae), synonym Scorzonera resedifolia L., is a medicinal plant used in the Libyan folkloric medicine mainly for the treatment of hepatic pains. However, there is no report on any pharmacological evaluation of L. resedifolia available to date. In this study, the neuropharmacological potential of the ethanol extract of this plant has been assessed in animal models. Launaea resedifolia extracts exhibited an inhibitory effect on the locomotor activity of mice in the open field test, an anti-nociceptive effect by increasing the hot plate reaction time in the hot plate test, and an anti-inflammatory activity in the carrageenan-induced paw oedema. Furthermore, a sedative effect was evident from the decrease in the onset of pentobarbitone sleeping time and increase in the duration of pentobarbitone sleeping time rats. The ethanol extract also demonstrated a significant decrease in the mortality rate induced by picrotoxin by about 66 percent, and a considerable reduction in the body weight of mice compared to the control groups.

Launaea resedifolia (L.) Kuntze (família: Asteraceae), sinônimo Scorzonera resedifolia L., é uma planta medicinal utilizada na medicina popular da Líbia para o tratamento de problemas hepáticos. Todavia, não há relato de qualquer avaliação farmacológica de L. resedifolia disponível na literatura. Neste estudo, o potencial neurofarmacológico do extrato etanólico desta planta foi analisado em modelos animais. Os extratos de Launaea resedifolia exibiram um efeito inibitório na atividade locomotora de camundongos no teste de campo aberto, um efeito anti-nociceptivo pelo aumento do tempo de reação em placa quente no teste da placa quente e uma atividade anti-inflamatória no edema de pata induzido por carragenina. Além disso, um efeito sedativo foi evidente pela diminuição no início do tempo de sono barbitúrico e aumento na duração do tempo de sono barbitúrico em ratos. O extrato etanólico também demonstrou uma diminuição significativa na taxa de mortalidade induzida por picrotoxina em aproximadamente 66 por cento e uma redução considerável no peso corporal de camundongos comparada aos grupos controle.