ABSTRACT
MicroRNAs play a critical role in regulating gene expression post-transcriptionally. Variations in mature microRNA sequences, known as isomiRs, arise from imprecise cleavage and nucleotide substitution or addition. These isomiRs can target different mRNAs or compete with their canonical counterparts, thereby expanding the scope of miRNA post-transcriptional regulation. Our study investigated the relationship between cis-acting single-nucleotide polymorphisms (SNPs) in precursor miRNA regions and isomiR composition, represented by the ratio of a specific 5'-isomiR subtype to all isomiRs identified for a particular mature miRNA. Significant associations between 95 SNP-isomiR pairs were identified. Of note, rs6505162 was significantly associated with both the 5'-extension of hsa-miR-423-3p and the 5'-trimming of hsa-miR-423-5p. Comparison of breast cancer and normal samples revealed that the expression of both isomiRs was significantly higher in tumors than in normal tissues. This study sheds light on the genetic regulation of isomiR maturation and advances our understanding of post-transcriptional regulation by microRNAs.
ABSTRACT
OBJECTIVE: We aim to explore the mediating effect of hypertensive disorders of pregnancy (HDP) on the relationship between pre-pregnancy body mass index (BMI) and the risk of preterm birth (PTB) in women with singleton live births. METHODS: Demographic and clinical data of 3,249,159 women with singleton live births were extracted from the National Vital Statistics System (NVSS) database in this retrospective cohort study. The associations between pre-pregnancy BMI and HDP, HDP, and PTB, and pre-pregnancy BMI and PTB were evaluated using univariate and multivariate logistic regression analyses, with odds ratios (ORs) and 95% confidence intervals (CIs). Structural equation modeling (SEM) was used to explore the mediating effect of HDP on the relationship between pre-pregnancy BMI and PTB. RESULTS: In total, 324,627 women (9.99%) had PTB. After adjustment for covariables, there were significant associations between pre-pregnancy BMI and HDP [OR = 2.07, 95% CI: 2.05-2.09)], HDP and PTB [OR = 2.54, 95% CI: (2.52-2.57)], and pre-pregnancy BMI and PTB [OR = 1.03, 95% CI: 1.02-1.03)]. The effect of pre-pregnancy BMI on PTB was significantly mediated by HDP with a mediation proportion of 63.62%, especially in women of different ages and having gestational diabetes mellitus (GDM) or not. CONCLUSION: HDP may play a mediating role in the effect of pre-pregnancy BMI on PTB risk. Women preparing for pregnancy should pay close attention to BMI, and pregnant women should monitor and develop interventions for HDP to reduce the risk of PTB.
Subject(s)
Hypertension, Pregnancy-Induced , Premature Birth , Pregnancy , Female , Infant, Newborn , Humans , Premature Birth/epidemiology , Premature Birth/etiology , Hypertension, Pregnancy-Induced/epidemiology , Body Mass Index , Retrospective Studies , Risk Factors , Live BirthABSTRACT
The flow field is one of the most important external conditions affecting the development of biofouling community on artificial reefs (ARs), especially the microbial community. In this article, we investigated the temporal dynamics of microbial communities between the stoss side and the lee side of ARs. The results showed that the composition and structure of microbial and macrobenthic communities between the stoss side and the lee side both presented obvious temporal variations. Microbial diversity and richness were higher on the stoss side than that on the lee side. There was a greater impact on bacterial and archaeal communities on temporal scale compared to that on micro-spatial scale, which was not suitable for the fungal community. The organism biomass, abundance and coverage of macrobenthic community on the lee side were higher than those on the stoss side, and the microbial diversity on the stoss side increased significantly with the organism coverage.
Subject(s)
Biofouling , Microbiota , Bacteria , Archaea , BiomassABSTRACT
Seasonal low-oxygen in marine ranching in the northern Yellow Sea has been one of the major environmental problems in coastal waters in recent years. Nitrogen (N) and phosphorus (P) are important nutrients, which are susceptible to the concentration of dissolved oxygen (DO). This article studied the effects of low-oxygen on nutrients represented by N and P fractions in marine ranching in the northern Yellow Sea. The results showed that there were significant layer differences in temperature and salinity during the low-oxygen period. In the seawater, the nutrient distribution in the death disaster zone of sea cucumbers and the non-disaster zone was similar, and DO had a strong positive correlation with dissolved inorganic nitrogen (DIN). In the sediment, significant regional differences existed in nutrient concentration, and the concentration of total phosphorus (TP) decreased significantly with the increase in DO content. The results showed that the sources and sinks of nitrogen and phosphorus nutrients were inconsistent in this zone, and migration and transformation of the existing form of nitrogen with the seasonal changes in the water environment was a main factor for N distribution. This study extended the understanding of the effects of seasonal low-oxygen on N and P.
Subject(s)
Nitrogen , Phosphorus , Nitrogen/analysis , Phosphorus/analysis , Oxygen , Seasons , Environmental Monitoring , ChinaABSTRACT
Skin microbiotas play a crucial role in the health, homeostasis, and immune function of amphibians. The contaminants in water could affect the structure and composition of microbial communities. The effects of coexisting pollutants on frogs cannot be adequately explained by a single exposure due to the coexistence of Cd and DEHP in the environment. Following exposure to Cd and/or DEHP, we examined the histological characteristics of Rana chensinensis tadpoles. We also used the 16S rRNA gene sequencing technique to assess the relative abundance of skin microbial communities among tadpoles from each treatment group. Our findings indicate that R. chensinensis' skin experienced some degree of injury due to exposure to Cd and DEHP, which led to the imbalance of their skin microbial community homeostasis and thus interfered with the normal trial status of the host. That may eventually lead to the decline of the amphibian population.
Subject(s)
Diethylhexyl Phthalate , Microbiota , Animals , Larva , Cadmium/toxicity , RNA, Ribosomal, 16S/genetics , Anura , RanidaeABSTRACT
Temperature is one of the important factors affecting the growth, development, and metamorphosis of amphibians. Endochondral ossification during metamorphosis plays a crucial role in amphibian survival and adaptation on land. In this study, we explored the effects of different temperature treatments on the growth, development, and endochondral ossification of Rana chensinensis tadpoles during metamorphosis. The results showed that high temperature exposure may affect the skeletal development of tadpoles during metamorphosis, such as reduction of bone length and ossification of limbs, thyroid gland damage and change of ossification-related genes expression levelsï¼and ultimately affect the movement and survival of tadpoles in the terrestrial environment. These results provide an experimental reference for further research on the effects of temperature on amphibian growth and development and provide an important theoretical basis for the decline of the amphibian population caused by temperature.
Subject(s)
Osteogenesis , Ranidae , Animals , Larva , Osteogenesis/genetics , Temperature , Thyroid Gland/metabolism , Metamorphosis, BiologicalABSTRACT
Multiple types of artificial reefs have been widely deployed in the coast of northern Yellow Sea, which can enhance fishery resources, restore coastal habitats and improve the marine environment. Meiofauna plays important ecological roles in marine ecosystem, but the response mechanism of meiofaunal community to different types of artificial reef is still poorly understood. In this study, we characterized the meiofaunal communities of concrete artificial reef habitat (CAR), rocky artificial reef habitat (RAR), ship artificial reef habitat (SAR) and adjacent natural habitat (NH) using 18S rRNA gene high-throughput sequencing technology, and explored the relationship of community-environment. The results showed that the diversity and community structure of meiofauna differed significantly on both spatial and temporal scales. Spatial differences were mainly contributed to the flow field effects and biological effects generated by artificial habitats, while temporal differences were driven by temperature (T) and dissolved oxygen (DO). The dominant taxa of meiofauna included arthropods, annelids, platyhelminths and nematodes. Platyhelminths were mainly positively influenced by artificial habitats but annelids were the opposite. Co-occurrence network analysis revealed that NH was more sensitive to environmental change than artificial habitat, while the performance of CAR and SAR were more stable. These results indicated that meiofauna can respond accordingly to different types of artificial habitats, and could be superimposed over the normal seasonal effects. The current study could provide fundamental data for understanding the response mechanism of meiofaunal community to different types of artificial habitats and a reference for assessments of the impact of artificial reefs on the marine environment.
Subject(s)
Ecosystem , Nematoda , Animals , High-Throughput Nucleotide Sequencing , Coral ReefsABSTRACT
Climate change such as global warming is considered a major threat to amphibians. The guts of amphibians are home to trillions of microbes, which are key regulators of gastrointestinal digestion and play a crucial role in lipid metabolites. The aim of this study was to evaluate the effect of temperature change on intestinal microbiota and lipid metabolism in Rana chensinensis tadpoles. Morphological and intestinal microbiota data of R. chensinensis larvae exposed to different temperatures (15 °C, 21 °C, and 26 °C) were measured. The results show that the warm temperature causes histological damage to the intestinal epithelium. In addition, temperature treatments alter the diversity and composition of gut microbes in R. chensinensis tadpoles. At the phylum level of intestinal microbial community, Campilobacterota was detected only in the warm group. At the genera level, unclassified_f__Enterobacteriaceae was markedly declined in the warm group but was notably enriched in the cold group. For lipid metabolism-related genes, the expression levels of GPR109A, HDAC1, and APOA-I decreased significantly in both warm and cold treatment groups, while the expression levels of CLPS and LIPASE increased significantly. Collectively, these observations demonstrated that warm and cold temperatures may reduce the immune capacity of tadpoles by changing the composition of intestinal microorganisms and the expression of genes related to lipid metabolism, affecting the survival of tadpoles.
Subject(s)
Gastrointestinal Microbiome , Animals , Larva , Temperature , Lipid Metabolism , RanidaeABSTRACT
Artificial reefs have been widely deployed in the northern Yellow Sea. However, the differences in the ecological benefits on different types of artificial reef habitats are still poorly understood. In this study, the temporal and spatial differences on benthic fish communities were evaluated among concrete artificial reef habitat (CAR), rocky artificial reef habitat (RAR), ship artificial reef habitat (SAR) around Xiaoshi Island in northern Yellow Sea. The results indicated that all three types of artificial reef habitats can enhance the diversity variables of benthic fish communities, and fish abundance, species richness and Shannon-Wiener index of CAR were generally better than the other two. CAR and RAR hosted similar community composition of benthic fish, and all types of habitats showed significant differences in community composition between winter-spring and summer-autumn. Environmental factors, especially water temperature, can also affect the community composition by affecting the migration of temperature-preferred species. Overall, the enhancement effects of artificial habitats on fisheries productivity varied with fish species and reef types. This study will help to understand the ecological effects of different types of artificial reefs in northern Yellow Sea, and then could give an insight for scientific construction of artificial reefs in this region.
Subject(s)
Ecosystem , Fishes , Animals , Coral Reefs , Fisheries , SeasonsABSTRACT
Macroalgae and their rocky substrates both support diverse and abundant microbiota, performing essential ecological functions in marine ecosystem. However, the differences in the epiphytic microbial communities on macroalgae and rocky substrate are still poorly understood. In this study, the epiphytic microbial communities on four macroalgae (Corallina officinalis, Rhodomela confervoides, Sargassum thunbergii, and Ulva linza) and their rocky substrates from Weihai coast zone were characterized using high-throughput sequencing technology. The results showed that the alpha diversity indices were greater in rocky substrates than that in macroalgae. The microbial similarities among macroalgae and rocky substrate groups tended to decrease from the high taxonomic ranks to lower ranks, only 22.69% of the total amplicon sequence variants (ASVs) were shared between them. The functional analysis revealed that the microbiotas were mainly involved in metabolic activities. This study would provide the theoretical foundation for macroalgal cultivation and algal reef applications.
Subject(s)
Microbiota , Rhodophyta , Seaweed , Ulva , Bacteria , EcosystemABSTRACT
Gastrointestinal tract and intestine microbiota can both have deep effects on the lipid metabolism and immune function of amphibians. Additionally, the composition and structure of the microbial community are influenced by environmental pollutions. It is noteworthy that environmental compounds such as Cd and DEHP are pervasive in the aquatic environment and do not exist in isolation, and single exposure experiments cannot well explain the effects of unpredictable interactions between co-existing compounds on amphibians. In this study, we calculated the parameters of morphological and histological indices of Rana chensinensis tadpoles after treated with Cd and/or DEHP. The 16S rRNA gene sequencing technology was used to assess the relative abundance of intestinal microbial community among tadpoles from each treatment groups. We also examined the mRNA expression levels of lipid digestion and absorption and SCFAs related-genes. Our results indicated that all morphological and histological indices were significantly declined in the Cd treatment group, while the mixed treatment group was similar to the control group. Compared with the control group, the relative abundances of Firmicutes, Proteobacteria and Verrucomicrobia exhibited distinctive differences in Cd and/or DEHP treatment groups. Further, RT-qPCR results revealed that the expression levels of lipid metabolism and SCFAs related-genes were also significantly altered among the treatment groups. Taken together, the present study highlighted a new evidence that the alterations in intestinal microbial community and mRNA expression levels of larval amphibians after exposure to Cd and/or DEHP may impair lipid storage and transport, as well as reduce anti-inflammatory capacity, which may ultimately lead to a decline in amphibian populations.
Subject(s)
Diethylhexyl Phthalate , Gastrointestinal Microbiome , Animals , Cadmium/metabolism , Diethylhexyl Phthalate/metabolism , Fatty Acids/metabolism , Larva , Lipid Metabolism , RNA, Ribosomal, 16S/genetics , RanidaeABSTRACT
Seasonal hypoxia, as an increasingly recognized environmental issue, frequently occurred in marine ranching from northern Yellow Sea, China. Although microorganisms play an important ecological role in marine ecosystems, but little is known on the response mechanism of microbial community to seasonal hypoxia in marine ranching. A total of 132 seawater samples and 47 sediment samples were collected from the marine ranching, both in the death disaster zone of sea cucumbers and the non-disaster zone, and in different months. 16S rRNA gene high-throughput sequencing was used to explore the microbial community and its influencing factors. The results showed that the stratification in community composition and dissolved oxygen content appeared in August. The Alpha diversity in seawater was higher in summer than in winter, and significant differences in Beta diversity appeared between the death disaster zone of sea cucumbers and the non-disaster zone in sediments. In addition, environmental effects explained more of the variation in bacterial community composition in seawater as compared with spatial effects did, whereas, sedimentary bacterial communities were more closely related to spatial effects. The present results could provide fundamental data for understanding the response mechanism of the microbial community to seasonal hypoxia in marine ranching and are of great significance for the management and protection of marine ranching.
Subject(s)
Microbiota , Geologic Sediments , Humans , Hypoxia , Phylogeny , RNA, Ribosomal, 16S/genetics , Seasons , SeawaterABSTRACT
Neoantigen peptides arising from genetic alterations may serve as targets for personalized cancer vaccines and as positive predictors of response to immune checkpoint therapy. Mutations in genes regulating RNA splicing are common in hematological malignancies leading to dysregulated splicing and intron retention (IR). In this study, we investigated IR as a potential source of tumor neoantigens in multiple myeloma (MM) patients and the relationship of IR-induced neoantigens (IR-neoAg) with clinical outcomes. MM-specific IR events were identified in RNA-sequencing data from the Multiple Myeloma Research Foundation CoMMpass study after removing IR events that also occurred in normal plasma cells. We quantified the IR-neoAg load by assessing IR-induced novel peptides that were predicted to bind to major histocompatibility complex (MHC) molecules. We found that high IR-neoAg load was associated with poor overall survival in both newly diagnosed and relapsed MM patients. Further analyses revealed that poor outcome in MM patients with high IR-neoAg load was associated with high expression levels of T-cell co-inhibitory molecules and elevated interferon signaling activity. We also found that MM cells exhibiting high IR levels had lower MHC-II protein abundance and treatment of MM cells with a spliceosome inhibitor resulted in increased MHC-I protein abundance. Our findings suggest that IR-neoAg may represent a novel biomarker of MM patient clinical outcome and further that targeting RNA splicing may serve as a potential therapeutic strategy to prevent MM immune escape and promote response to checkpoint blockade.
Subject(s)
Antigens, Neoplasm/genetics , Biomarkers, Tumor/genetics , Multiple Myeloma/genetics , Sequence Analysis, RNA/methods , Cell Line, Tumor , Female , Gene Expression Regulation, Neoplastic , Gene Regulatory Networks , Humans , Introns , Male , Mutation , Prognosis , RNA Splicing , Survival AnalysisABSTRACT
We conducted a large-scale epidemiological investigation to detect the prevalence of Toxoplasma gondii in four marine bivalve shellfish species collected from six representative coastal regions of Weihai, eastern China. Between January 2018 and December 2018, 14,535 marine bivalve shellfish pooled into 2907 samples were randomly collected and examined for T. gondii DNA by a nested PCR assay targeting B1 gene. The results showed that 2.8% (82) of the 2907 pooled samples were tested positive for T. gondii DNA. Two T. gondii genotype (ToxoDB Genotype #9 and ToxoDB Genotype #1) were identified PCR-restriction fragment length polymorphism analysis. Factors that were found significantly associated with the presence of T. gondii DNA in marine bivalve shellfish included the source of samples (being wild) (odds ratio [OR], 3.34; 95% confidence interval [CI], 2.00-5.84; p < 0.01), surface runoff near the sampling site (OR, 2.64; 95% CI, 1.47-4.72; p < 0.01), and presence of cats near the sampling site (OR, 1.77; 95% CI, 1.02-3.07; p = 0.04). Moreover, the prevalence of T. gondii DNA in marine bivalve shellfish correlated with temperature (Pearson's correlation: R = 0.75, p = 0.0049) and precipitation (R = 0.87, p = 0.00021). These findings provide new insights into the presence of T. gondii DNA in marine bivalve shellfish and highlight the impact of human activity on marine pollution by such an important terrestrial pathogen pollutant.
Subject(s)
Bivalvia/parasitology , Toxoplasmosis, Animal/epidemiology , Animals , Bivalvia/genetics , China/epidemiology , Genotype , Humans , Polymerase Chain Reaction/methods , Prevalence , Risk Factors , Shellfish , Toxoplasma/geneticsABSTRACT
Concrete and wood are commonly used to manufacture artificial reefs (ARs) worldwide for marine resource enhancement and habitat restoration. Although microbial biofilms play an important role in marine ecosystems, the microbial communities that colonize concrete and wooden ARs and their temporal succession have rarely been studied. In this study, the temporal succession of the microbial communities on concrete and wooden AR blocks and the driving factors were investigated. The composition of the microbial communities underwent successive shifts over time: among the six dominant phyla, the relative abundances of Proteobacteria, Cyanobacteria and Gracilibacteria significantly decreased in wood, as did that of Cyanobacteria in concrete. Operational taxonomic units (OTU) richness and Shannon index were significantly higher in concrete than in wood. Non-metric multidimensional scaling ordination placed the microbial communities in two distinct clusters corresponding to the two substrate materials. The macrobenthic compositions of concrete and wood were broadly similar and shifted over time, especially in the first five weeks. The Shannon index of the microbial communities in concrete and wood increased significantly with the organism coverage. The results provide fundamental data on microbial community succession during the initial deployment of ARs and contribute to understanding the ecological effects of ARs.
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Increasing evidence exisits for the role that shellfish play in the epidemiology of Toxoplasma gondii in marine environment. However, limited information is available on the level of T. gondii infection in wild marine snails, which can play a role in the transmission of T. gondii to other marine organisms and humans. In this study, the prevalence of T. gondii DNA in wild marine snails collected from three coastal cities in China was determined. Between January 2018 and November 2019, 1,206 wild marine snails were randomly collected and examined for the presence of T. gondii DNA using a nested polymerase chain reaction (PCR) targeting T. gondii B1 gene. The amplified products were genotyped using multilocus PCR-restriction fragment length polymorphism analysis. We also examined whether species of snail, sampling region, sampling season, surface runoff near samplic site, residential water discharge near samplic site, and proximity to livestock farms are associated with the occurrence of T. gondii DNA in marine snails. Our results showed that 23 (1.91%) snails were positive for T. gondii B1 gene. The genotype of two of the 23 T. gondii amplicons was consistent with ToxoDB Genotype #9. Multiple logistic regression revealed that surface runoff near the sampling site (P = 0.039, odds ratio [OR] = 3.413, 95% confidence interval [CI]: 1.07-10.94) and residential water discharge near the sampling site (P = 0.021, OR = 3.990, 95%CI: 1.24-12.87) are more likely to be associated with the presence of T. gondii DNA in marine snails. The detection of T. gondii DNA in marine snails in China highlights the potential impact of the anthropogenic activities on marine organisms and the potential foodborne risk posed to humans with such an important terrestrial pathogen.
Subject(s)
DNA, Protozoan/isolation & purification , Genotype , Snails/parasitology , Toxoplasma/genetics , Animals , China/epidemiology , DNA, Protozoan/genetics , Humans , Logistic Models , Odds Ratio , Polymerase Chain Reaction/veterinary , Risk Factors , Toxoplasma/isolation & purificationABSTRACT
Invasive plants often change a/biotic soil conditions to increase their competitiveness. We compared the microbially mediated soil nitrogen (N) cycle of invasive Mikania micrantha and two co-occurring native competitors, Persicaria chinensis and Paederia scandens. We assessed how differences in plant tissue N content, soil nutrients, N cycling rates, microbial biomass and activity, and diversity and abundance of N-cycling microbes associated with these species impact their competitiveness. Mikania micrantha outcompeted both native species by transferring more N to plant tissue (37.9-55.8% more than natives). We found total soil N to be at lowest, and available N highest, in M. micrantha rhizospheres, suggesting higher N cycling rates compared with both natives. Higher microbial biomass and enzyme activities in M. micrantha rhizospheres confirmed this, being positively correlated with soil N mineralization rates and available N. Mikania micrantha rhizospheres harbored highly diverse N-cycling microbes, including N-fixing, ammonia-oxidizing and denitrifying bacteria and ammonia-oxidizing archaea (AOA). Structural equation models indicated that M. micrantha obtained available N via AOA-mediated nitrification mainly. Field data mirrored our experimental findings. Nitrogen availability is elevated under M. micrantha invasion through enrichment of microbes that participate in N cycling, in turn increasing available N for plant growth, facilitating high interspecific competition.
Subject(s)
Mikania , China , Introduced Species , Nitrogen , Soil , Soil MicrobiologyABSTRACT
Mikania micrantha is one of the top 100 worst invasive species that can cause serious damage to natural ecosystems and substantial economic losses. Here, we present its 1.79 Gb chromosome-scale reference genome. Half of the genome is composed of long terminal repeat retrotransposons, 80% of which have been derived from a significant expansion in the past one million years. We identify a whole genome duplication event and recent segmental duplications, which may be responsible for its rapid environmental adaptation. Additionally, we show that M. micrantha achieves higher photosynthetic capacity by CO2 absorption at night to supplement the carbon fixation during the day, as well as enhanced stem photosynthesis efficiency. Furthermore, the metabolites of M. micrantha can increase the availability of nitrogen by enriching the microbes that participate in nitrogen cycling pathways. These findings collectively provide insights into the rapid growth and invasive adaptation.
Subject(s)
Genome, Plant , Mikania/growth & development , Mikania/genetics , Mikania/physiology , Biosynthetic Pathways/genetics , Biosynthetic Pathways/physiology , Carbon Cycle , Carbon Dioxide/metabolism , Chromosomes, Plant , Ecology , Ecosystem , Evolution, Molecular , Genomics , Introduced Species , Nitrogen/metabolism , Nitrogen Cycle , Photosynthesis/physiology , Plant Leaves/growth & development , Plant Leaves/metabolism , Sequence Analysis, DNA , TranscriptomeABSTRACT
In this study, the complete mitochondrial genome of the hybrid of Haliotis discus hannai (â) × Haliotis iris (â) was sequenced and analyzed for the first time. The mitogenome had a length of 16,719 bp and contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and 1 control region (CR). The sequence identity of the complete mtDNA sequences between the hybrid F1 and H. discus hannai was 99.40%, which revealed that the mitogenome of the hybrid subjected to the maternal inheritance rule. The phylogenetic analysis also presented that the hybrid F1 was relatively more close to H. discus hannai.
ABSTRACT
OBJECTIVE: The Carbon fiber reinforced carbon matrix composites were employed for reconstruction of large circumferential defect of the cervical trachea. The biocompatibility and biofunctionality of the new type carbonaceous tracheal prosthesis were evaluated, and the feasibility for cervical tracheal reconstruction discussed. METHODS: Two types of carbonaceous tracheal prosthesis with different weaving methods of carbon fiber were used on eight healthy canines. Three to six tracheal cartilage rings were resected circumferentially. The 2 cm long tracheal prosthesis was transplanted into canines and the anastomosis was completed by end-to-end, tracheal-into-prosthesis and prosthesis-into-tracheal method. The function of breathing, eating and infection was observed after surgery. Four months later, the five survival canines were sacrificed and the prosthesis with surrounding tissues was removed for observation by optical microscopy and scanning electron microscopy. RESULTS: All dogs had cough symptom in different degree lasted 1 - 4 weeks after surgery. Two dogs with tracheal-into-prosthesis anastomosis showed eating disorders in different degree. One canine died due to airway obstruction caused by dislocation of prosthesis within three weeks after operation. Another two deaths in 11th week and 12th week were attributed to suffocation because of hypergranulation and scar formation. Prosthesis was surrounded by connective tissues and anchored firmly to the neighboring tissues. Most part of the luminal surface of tracheal prosthesis was not covered by respiratory mucosa. However, the inner layer showed scant re-epithelialization beyond the anastomosis. CONCLUSIONS: The implantation of the carbonaceous tracheal prosthesis can maintain the normal respiratory function of the experimental canines, but hypergranulation and scar formation around the end of the tracheal prosthesis and epithelium on the luminal surface of the prosthesis are questions still remained to be solved.