Rapid visualization molecular fluorescence detection of methicillin-resistant Staphylococcus aureus using the multiplex MIRA-qPCR method.

Multidrug-resistant (MDR) bacterial infections constitute a major public health problem worldwide. A rapid method for the detection of methicillin-resistant Staphylococcus aureus (MRSA) is critical for the timely prevention of bacterial infections and the accurate clinical use of drugs. The nuc and mecA genes are potentially indicative of MRSA infection and in this study, a multiplex molecular fluorescence multi-enzyme isothermal rapid amplification visual assay was proposed and established. The method is capable of detecting MRSA at 17 min, 40°C amplification, and is well differentiated from common clinical bacteria in specific assays, with 500 colony-forming units (CFU) mL-1 of MRSA detected under optimal conditions. This method has excellent diagnostic capabilities versus classical methods to detect clinical samples and shows potential in the identification of pathogenic microorganisms in a clinical setting.

Hearing healthcare utilisation among older adults with self-reported hearing loss during the COVID-19 pandemic in the United States.

OBJECTIVE: To ascertain the prevalence, causes, and risk factors of hearing healthcare delays in older people with self-reported hearing loss in the United States. DESIGN: This cross-sectional study used data from the National Health and Ageing Trends Study (NHATS), a nationally representative survey of Medicare beneficiaries. A supplemental COVID-19 survey was mailed to the participants from June to October 2020. STUDY SAMPLE: By January 2021, 3257 participants had returned completed COVID-19 questionnaires, with the majority having been self-administered between July and August 2020. RESULTS: The participants in the study represented 32.7 million older adults in the US, with 29.1% reporting hearing loss. Among over 12.4 million older adults who put off needed or planned medical care, 19.6% of those with self-reported hearing loss and 24.5% of hearing aid or device users stated they delayed hearing appointments. Approximately 629,911 older adults with hearing devices were impacted by the COVID-19 outbreak for audiological services. The top three reasons were deciding to wait, service cancellation, and fear of going. Education and race/ethnicity were associated with delaying hearing healthcare. CONCLUSIONS: The COVID-19 pandemic impacted hearing healthcare utilisation among older adults with self-reported hearing loss in 2020, with both patient- and provider- initiated delays.

Mechanism insights into enhanced treatment of wasted activated sludge by hydrogen-mediated anaerobic digestion.

In the current study, different forms of added gas including H2, CO2, and mixed gas (VH2:VCO2 = 4:1), as well as different hydrogen partial pressures (0.10, 0.30, and 0.50 atm) were investigated for the influence on anaerobic performance in waste activated sludge (WAS) treatment. The mixed gas significantly improved methane production by over 20%, which positively correlated with the hydrogen partial pressure. However, pure H2 (0.5 atm) heavily inhibited methane production by 76.5%. Combined with the microbial metabolic activity study, H2 accelerated the hydrolysis process. Afterward, mixing with CO2 accelerated H2 and organic consumption, thus promoting WAS degradation and methane production. Based on the most extra release of organics, the mixed group exerted the superior performance with hydrogen partial pressure at 0.3 atm. The microbial community analysis evidenced that mixed gas enriched proteolytic and homoacetogenic bacteria and hybrid-trophic methanogens. By metagenomics study, hydrolysis, acetogenic, and methanogenesis pathways were all enhanced via the exogenous addition of H2 and CO2, sustainably transforming WAS towards CH4. This study discovered the mechanism of the enhanced conversion from WAS to CH4 by exogenous H2 and provided a promising approach for WAS reduction and energy recovery.

CpMAX1a, a Cytochrome P450 Monooxygenase Gene of Chimonanthus praecox Regulates Shoot Branching in Arabidopsis.

Strigolactones (SLs) are a class of important hormones in the regulation of plant branching. In the model plant Arabidopsis, AtMAX1 encodes a cytochrome P450 protein and is a crucial gene in the strigolactone synthesis pathway. Yet, the regulatory mechanism of MAX1 in the shoot branching of wintersweet (Chimonanthus praecox) remains unclear. Here we identified and isolated three MAX1 homologous genes, namely CpMAX1a, CpMAX1b, and CpMAX1c. Quantitative real-time PCR (qRT-PCR) revealed the expression of CpMAX1a in all tissues, being highest in leaves, whereas CpMAX1b was only expressed in stems, while CpMAX1c was expressed in both roots and stem tips. However, CpMAX1a's expression decreased significantly after decapitation; hence, we verified its gene function. CpMAX1a was located in Arabidopsis chloroplasts. Overexpressing CpMAX1a restored the phenotype of the branching mutant max1−3, and reduced the rosette branch number, but resulted in no significant phenotypic differences from the wild type. Additionally, expression of AtBRC1 was significantly upregulated in transgenic lines, indicating that the CpMAX1a gene has a function similar to the homologous gene of Arabidopsis. In conclusion, our study shows that CpMAX1a plays a conserved role in regulating the branch development of wintersweet. This work provides a molecular and theoretical basis for better understanding the branch development of wintersweet.

Ovarian Response, Pregnancy Outcomes, and Complications Between Salpingectomy and Proximal Tubal Occlusion in Hydrosalpinx Patients Before in vitro Fertilization: A Meta-Analysis.

Objectives: Contradictory findings exist in studies comparing salpingectomy and proximal tubal occlusion (PTO) in treating hydrosalpinx patients before in vitro fertilization (IVF). Therefore, this meta-analysis aimed to comprehensively compare ovarian response, pregnancy outcomes, and complications between salpingectomy and PTO in treating these patients. Methods: Embase, PubMed, and Web of Science were searched to identify relevant articles published from 1980 to August 31, 2020. Eight studies that involve 716 hydrosalpinx patients before IVF were included, among whom 408 patients received salpingectomy and 308 patients received PTO. The data were pooled; the standardized mean difference (SMD) or odds ratio (OR) was calculated. Results: Proximal tubal occlusion-treated patients had higher fertilization rate (SMD = 0.35, 95% CI: 0.11-0.59), while similar days of controlled ovarian hyperstimulation (COH) (SMD: 0.15, 95% CI: -0.36-0.67) and number of retrieved oocytes (SMD = -0.22, 95% CI: -0.54-0.10) compared with salpingectomy-treated patients. Furthermore, no difference of implantation rate (OR = 1.17, 95% CI: 0.62-2.20), clinical pregnancy rate (OR = 0.82, 95% CI: 0.59-1.15), ongoing pregnancy rate (OR = 0.64, 95% CI: 0.36-1.13), or live birth rate (OR = 0.67, 95% CI: 0.16-2.72) was shown between salpingectomy-treated patients and PTO-treated patients. Additionally, ectopic pregnancy rate (OR = 1.13, 95% CI: 0.21-5.92) and miscarriage rate (OR = 0.88, 95% CI: 0.31-2.48) were similar between salpingectomy-treated patients and PTO-treated patients. Conclusion: Proximal tubal occlusion exhibits a higher fertilization rate but no obvious benefits on days of COH, number of retrieved oocytes, pregnancy outcomes, and complications over salpingectomy in hydrosalpinx patients before IVF.

A Pilot Study to Develop the Rapid Estimate of Adult Literacy in Audiology.

BACKGROUND: Health literacy describes an individuals' ability to maximize their potential in health care, including one's ability to understand information needed to make informed health decisions. A variety of general and condition-specific health literacy assessment tools have been created to help health professionals assess patients' health literacy skills and tailor the need for health care communication or education; however, there are no such tools available for the audiology field. OBJECTIVE: The purpose of the study was to develop an objective reading recognition audiology-related health literacy assessment tool, the Rapid Estimate of Adult Literacy in Audiology (REALA). METHODS: This was a cross-sectional study (N = 200). The initial version of the REALA contained 99 words specifically related to audiology. The final version, revised to have improved clinical utility, contained a total of 48 words that were selected based on item difficulty, item discrimination score, and point-biserial index using classical item analysis. KEY RESULTS: The total pass rate for the final version of the 48-word REALA was 0.72 (standard deviation = 0.45) and the Cronbach coefficient alpha was 0.93. Once the comprehension component is added to the tool, the REALA can be a valuable health literacy assessment tool that health professionals use to evaluate patients' audiology-related health literacy. CONCLUSION: Once the comprehension component is added to the tool, the REALA can be a valuable health literacy assessment tool that health professionals use to evaluate patients' audiology-related health literacy. [HLRP: Health Literacy Research and Practice. 2022;6(2):e88-e95.] Plain Language Summary: A health literacy assessment tool, the REALA, was developed in the study. The final version of REALA contained 48 words relative to hearing healthcare. The results suggested that REALA can help health professionals assess patients' hearing related health literacy and tailor the need for hearing health care communication or education.

Promoting direct interspecies electron transfer and acetoclastic methanogenesis for enhancing anaerobic digestion of butanol octanol wastewater by coupling granular activated carbon and exogenous hydrogen.

Butanol octanol wastewater (BOW) generated from syngas conversion of coal contained abundant toxic organic pollutants. Anaerobic digestion is a promising technology for BOW, but abundant toxic substances would inhibit the activity of anaerobic microorganisms. Granular activated carbon (GAC) and exogenous hydrogen (EH2) were employed to enhance anaerobic digestion of BOW. The results indicated that methane production increased to 289.55 ± 17.43 mL CH4/g COD in EH2/GAC group, which was 1.07, 2.04, and 1.98 times of that in GAC, EH2, and control groups, respectively. In EH2/GAC group. The relative abundance of Geobacter and Methanosaeta increased rapidly to 25.36% and 52.81%, respectively, and the relative abundance of Clostridium was 9.78%. The underlying mechanism might be that GAC promoted the enrichment of Geobacter, and EH2 changed metabolic mechanism of Clostridium, stimulating the enrichment of Methanosaeta. Direct interspecies electron transfer was promoted by EH2/GAC, thus improving the methane production rate of BOW.

Phgdh serves a protective role in Il­1ß induced chondrocyte inflammation and oxidative­stress damage.

The primary pathological changes observed in osteoarthritis (OA) involve inflammation and degeneration of chondrocytes. 3­phosphoglycerate dehydrogenase (Phgdh), a rate­limiting enzyme involved in the conversion of 3­phosphoglycerate to serine, serves as a crucial molecular component of cell growth and metabolism. However, its effects on chondrocytes in OA have not been determined. In the present study, a rat model of OA was used to investigate the expression levels of Phgdh in vivo and in vitro. Additionally, the role of Phgdh in extracellular matrix (ECM) synthesis, inflammation, apoptosis and oxidative stress levels of chondrocytes was detected in vitro. Phgdh expression was decreased in OA, and Phgdh overexpression promoted ECM synthesis, decreased levels inflammatory cytokines, such as Il­6, TNF­α, a disintegrin and metalloproteinase with thrombospondin motifs 5 and MMP13, and decreased apoptosis. Furthermore, expression of Phgdh effectively increased expression levels of the cellular antioxidant enzymes catalase and superoxide dismutase 1, and decreased the levels of reactive oxygen species in chondrocytes; and this may have been regulated by a Kelch like ECH associated protein 1/nuclear factor erythroid 2­related factor 2 axis. Taken together, these results suggest that Phgdh may be used to manage the progression of OA.

Coupling granular activated carbon and exogenous hydrogen to enhance anaerobic digestion of phenol via predominant syntrophic acetate oxidation and hydrogenotrophic methanogenesis pathway.

Anaerobic digestion is a promising biological method for treating phenol-containing wastewater. However, the low methane yield of phenol due to the biological toxicity limits its potential application. This study presents a novel method to enhance the conversion rate of phenol to methane by coupling of granular activated carbon and exogenous hydrogen (GAC/H2). The cumulative methane production in the GAC/H2, H2, GAC, and control groups were 408.2 ± 16.2, 336.5 ± 5.7, 287.2 ± 26. 2, and 258.1 ± 8.6 mL‬ CH4/g COD, respectively. Compared with the control group, the hydrogenotrophic methanogenic activity and electron transfer activity of GAC/H2 group were increased by 403.9 and 367.4%, respectively. The results of the 16SrRNA analysis indicated GAC enhanced the relative abundances of Syntrophus and Syntrophorhabdus, and hydrogen promoted the relative abundances of Cryptanaerobacter, Aminicenantes, and Methanobacterium. Therefore, the coupling of GAC and exogenous hydrogen promoted a dominate SAO-HM pathway to convert phenol to methane.

Do We Have Effective Patient Education Materials for Age-Related Hearing Loss?

Purpose The purpose of this study was to explore and compare the readability and suitability of patient education materials (PEMs) on topics of age-related hearing loss (ARHL) supplied by electronic health record (EHR) systems and organizations specializing in communication sciences and disorders (CSD). Method PEMs on ARHL were identified through a computerized search of EHR databases and CSD organization websites. Selected PEMs were assessed using three readability indices as well as the Suitability Assessment of Materials (SAM; Doak et al., 1996), which is a standardized tool to assess the content and design of written educational materials. Ten PEMs from EHR databases and 17 PEMs from CSD organizations were analyzed. Results Overall, 66.7% of PEMs were written above the eighth-grade readability target. PEMs from CSD organizations were significantly more difficult to read compared to those from EHR databases. In total, 85.2% of PEMs were classified as "adequate" using the SAM analysis. No significant SAM score differences were found between PEMs from CSD organizations and those from EHR databases. Common areas of weakness among PEMs were (a) failure to include a summary of key information, (b) reading level, (c) vocabulary (too advanced for the intended audience), and (d) limited subdivision of complex topics. Conclusions The readability and suitability of PEMs on topics of ARHL supplied by EHR providers and CSD organizations are not supportive of the health literacy skills of the average U.S. adult. It is critical to improve the readability, suitability, and comprehensibility of PEMs on ARHL to make information about hearing health care more accessible and usable.

Development of a Revised Performance-Perceptual Test Using Quick Speech in Noise Test Material and Its Norms.

BACKGROUND: Two audiometric speech measures have been recognized to have associations with hearing aid use success: the Quick Speech in Noise (QuickSIN) test and the Performance-Perceptual Test (PPT). The PPT involves using the same speech test material (Hearing in Noise Test [HINT]) twice, to evaluate patients' objective and subjective speech recognition performance in noise and the discrepancy between the two measures (Performance-Perceptual Discrepancy [PPDIS]). Using the QuickSIN to conduct the PPT (revised-PPT) may provide clinicians two pieces of important information from one test to help predict hearing aid use success and the need for counseling. Moreover, we could achieve the desired clinical efficiency without purchasing additional test materials. PURPOSE: This study aimed (1) to evaluate the validity and reliability of using the QuickSIN speech material to administer the PPT and (2) to establish normative data across listeners with normal hearing (NH) and hearing loss (HL). RESEARCH DESIGN: This study used a repeated measures design. STUDY SAMPLE: Of the total 65 participants between 18 and 88 years of age, 20 (31%) had NH and 45 (69%) had sensorineural HL, ranging from mild to profound in both ears. Thirty-two of the 45 participants with HL were hearing aid users. DATA COLLECTION AND ANALYSIS: All participants completed the original PPT using HINT and the revised-PPT using QuickSIN, via sound field. Generalized linear mixed models were used to compare the performance, perceptual, and PPDIS data between the two test materials across all participants. Normative data for the revised-PPT were established from all participants. RESULTS: Significant main effects for both the test material and hearing status were found for the performance and perceptual data. All interactions were nonsignificant. There were no significant PPDIS differences between the original PPT and the revised-PPT. Normative values for the revised-PPT were established and comparable to the norms for the original PPT in the present study. The test-retest results suggested that the revised-PPT has good reliability. In addition, it appeared that there was a negative association between underestimation of hearing ability and hearing aid use success. CONCLUSIONS: It is concluded that the QuickSIN speech material can replace HINT to measure PPT. The revised-PPT may serve as a useful and efficient clinical tool in any clinics for hearing aid fitting and counseling.

Efficacy of aspirin, prednisone, and multivitamin triple therapy in treating unexplained recurrent spontaneous abortion: A cohort study.

OBJECTIVE: To assess the efficacy of aspirin, prednisone, and multivitamin triple therapy in treating unexplained recurrent spontaneous abortion (URSA). METHODS: Data were reviewed from women with early RSA attending a hospital in Beijing, China, 2013-2017. Those with no abnormal indices (e.g., endocrine, coagulation, immune, genetic) were diagnosed as having URSA, and received aspirin, prednisone, and multivitamin therapy (triple therapy group, n=106) or folic acid monotherapy (control group, n=65). Treatment efficacy was evaluated as the rate of successful treatment (12-week pregnancy with obvious embryo and embryonic heart, nuchal translucency thickness <0.25 cm, size consistent with gestational age, no early malformation). RESULTS: Overall, 362 women had early RSA and 171 (47.2%) had URSA. The rate of successful pregnancy was similar between the triple therapy (89.6%) and control (92.3%) groups (P=0.343). The rate of successful treatment was higher in the triple therapy (86.3%) than in the control (53.3%) group (P<0.001). In multivariate logistic regression analysis, triple therapy was associated with higher odds of successful treatment, whereas the number of spontaneous pregnancy losses was associated with lower odds of successful treatment. CONCLUSION: The triple therapy of aspirin, prednisone, and multivitamin was found to be a good treatment option for women with URSA.

Intraspinal neural axis abnormalities in severe spinal deformity: a 10-year MRI review.

PURPOSE: Documents indicated that the average prevalence of intraspinal neural axis abnormalities (INAAs) in presumed idiopathic scoliosis (PIS) patients was about 17.7%. However, paucity study focuses on the incidence of INAAs in severe spinal deformity (SSDs). In this study, we investigate the incidence of intraspinal neural axis abnormalities (IINAAs) and the clinical relevance in SSD at a single center. METHODS: All the patients with SSDs admitted for spinal surgery were evaluated from 2003 to 2014. INCLUSION CRITERIA: patients who present with coronal Cobb over 90° (and/or the sagittal Cobb ≥ 90°); patients with whole spine magnetic resonance imaging (MRI) done preoperatively; and patients with documented clinical findings preoperatively. EXCLUSION CRITERIA: ankylosing spondylitis, adult onset scoliosis, scoliosis secondary to bone destruction, and spinal dysraphism. RESULTS: 101 patients fulfilled the criteria were included. 43 patients were detected with INAAs (42.6%, 43/101). The most common INAAs was syrinx (S) (16/43, 37.2%). Of which, 43.7% (7/16), 37.5% (6/16), and 18.7% (3/16) were spindle, slit, and swelling types, respectively. Most of them were located in thoracic (6/16, 37.5%) and cervical (5/16, 31.3%) region. MRI revealed Chiari malformation with syringomyelia (C + S) in ten patients (10/43, 23.2%), Chiari malformation (C) in 6 patients (6/43, 13.9%) and others in 11 patients (11/43, 25.6%). As to the etiology, most patients with INAAs were PIS (34/43, 79.1%). On clinical examination, 16 of 101 patients (16/101, 15.8%) had abnormal neurologic signs. 15 of 16 patients (15/16, 93.7%) with abnormal neurologic signs had INAAs on MRI. On the other hand, 28 of 43 patients (28/43, 65.1%) with INAAs on MRI presented neurologically intact. 28 of 85 patients (28/85, 32.9%) with neurologically intact were detected with INAAs on MRI. CONCLUSION: The incidence of INAAs in SSDs was 42.6%. 65.1% of them present intact neurologic status. The most common neural anomaly was syrinx. Preoperative whole spine MRI must be beneficial for SSDs even in the absence of neurological findings. These slides can be retrieved under Electronic Supplementary Material.

Development of a Shortened Version of the Spatial Hearing Questionnaire (SHQ-S) for Screening Spatial-Hearing Ability.

PURPOSE: The Spatial Hearing Questionnaire (SHQ) was developed to address how to measure spatial-hearing ability in complex listening situations (Tyler, Perreau, & Ji, 2009). It has been translated and validated into various languages, including Chinese, Dutch, French, and Persian. Although the SHQ contains only 24 items, it could be time-consuming in a busy clinic to administer. The purposes of this study were to develop and validate a shortened version of the SHQ (SHQ-S) and to compare self-perceived spatial-hearing ability across adults with normal hearing (NH), hearing loss (HL), and cochlear implants (CIs). METHOD: This was a retrospective study. The full version of the SHQ was administered to measure self-perceived spatial-hearing ability for 51 adults with NH at Augustana College, 47 adults with essentially mild to moderately severe sensorineural HL at Illinois State University, and 72 adult CI users at the University of Iowa. Exploratory factor analysis was performed for the full version for the data collected from adults with NH and HL. Appropriate items were chosen to develop the SHQ-S from the results of the exploratory factor analysis. Confirmatory factor analysis was then applied to test the factor structure of the SHQ-S for all participants. One-way analysis of variance was used to compare the self-perceived spatial-hearing performance scores between the 3 groups. RESULTS: The exploratory factor analysis revealed scores loaded on 2 factors. Six items from the full version were chosen accordingly. The results of the confirmatory factor analysis indicated that that a shortened version of 6 items is sufficient to measure spatial-hearing ability. The internal consistency reliability of the SHQ-S was high. The main effect of the one-way analysis of variance was significant for the groups, F(2, 167) = 36.0, p < .0001. The comparisons with the Tukey adjustment indicated that the NH group reported significantly better spatial-hearing ability than either the HL or the CI group (both adjusted p values < .05). There was no significant difference between the participants with HL and CI users. CONCLUSIONS: The psychometric characteristics of the 6-item SHQ-S were similar to those of the full version of the SHQ. We conclude that the SHQ-S is a reliable and valid tool for measuring spatial-hearing ability and screening for spatial-hearing difficulties. Participants with NH reported better spatial-hearing ability than those with HL or with CIs, whereas the CI users and participants with HL perceived similar spatial-hearing ability in the present study.

The Effects of Parent-Focused Slow Relaxed Speech Intervention on Articulation Rate, Response Time Latency, and Fluency in Preschool Children Who Stutter.

Purpose: This study investigated the effects of an intervention to reduce caregivers' articulation rates with children who stutter on (a) disfluency, (b) caregiver and child's articulation rates, and (c) caregiver and child's response time latency (RTL). Method: Seventeen caregivers and their preschool children who stuttered participated in a group study of treatment outcomes. One speech sample was collected as a baseline, and 2 samples were collected after treatment. Posttreatment samples were of caregivers speaking as they typically would and using reduced articulation rates. Results: Caregivers reduced articulation rates significantly in the 2 posttreatment samples, and a significant decrease of stuttering-like disfluencies (SLD) was found in the children in those 2 samples. No direct relationship was found between the caregiver's articulation rate and RTL, and there was a small correlation of RTL with the lower levels of SLD found postintervention. No significant relationships were found between the reduced levels of SLD and articulation rates for either caregivers or children. Conclusions: Results suggest caregivers can be trained to slow their speech, and children increased their fluency at the end of a program designed to slow caregiver articulation. The intentionally slower rate of the caregivers, however, was not significantly related to fluency.

Effectiveness and intermediates of microcystin-LR degradation by UV/H2O2 via 265 nm ultraviolet light-emitting diodes.

Although the degradation of cyanotoxins by 254 nm UV/H2O2 has been well elucidated, the efficiency and mechanism involved are not necessarily true for other UV wavelengths. The degradation of microcystin-LR (MC-LR), a representative cyanotoxin, was explored by UV/H2O2 using 265 nm ultraviolet light-emitting diode (UV-LED). The results indicated that 265 nm UV/H2O2 treatment had a high removal efficiency of MC-LR ([MC-LR] = 0.1 µM, apparent rate constants reached 0.2077 min-1, half-time at 3.3 min). The qualitative analyses demonstrated that three novel intermediates, C48H74N10O15 (molecular weight = 1030.5335), C36H58N10O14 (854.4134), and C33H54N10O14 (814.3821), were generated in 265 nm UV/H2O2. Five published intermediates were also confirmed. The generative pathway of these products mainly involved free hydroxyl radical oxidation, resulting in consecutive hydroxyl substitutions and hydroxyl additions of unsaturated bonds in MC-LR. The toxicity of MC-LR was weaken with a relative low mineralization. The electrical energy per order values were calculated to be in the range of 0.00447 to 0.00612 kWh m-3 order-1 for 100-5000 µg L-1 MC-LR. Overall, 265 nm UV-LED/H2O2 can be used as an alternative effective technology to improve the removal efficiency of MC-LR in water.

Degradation of ciprofloxacin by 280 nm ultraviolet-activated persulfate: Degradation pathway and intermediate impact on proteome of Escherichia coli.

In this study, the degradation of ciprofloxacin (CIP) was explored using ultraviolet activated persulfate (UV/PS) with 280 nm ultraviolet light-emitting diodes (UV-LEDs), and the toxicological assessment of degrading intermediates was performed using iTRAQ labeling quantitative proteomic technology. The quantitative mass spectrum results showed that 280 nm UV/PS treatment had a high transformation efficiency of CIP ([CIP] = 3 µM, [S2O82-] = 210 µM, apparent rate constants 0.2413 min-1). The high resolution mass spectrum analyses demonstrated that the primary intermediates included C15H16FN3O3 (m/z 306.1248) and C17H18FN3O4 (m/z 348.1354). The former one was formed by the cleavage of piperazine ring, while the later one was generated by the addition of a hydroxyl on the quinolone backbone. The toxicological assessment demonstrated that 56 and 110 proteins had significant up regulations and down regulations, respectively, in the Escherichia coli exposed to degraded CIP compared to untreated CIP. The majority of up-regulated proteins, such as GapA, SodC, were associated with primary metabolic process rather than responses to stress and toxic substance, inferring that the moderate UV/PS treatment can reduce the antibacterial activity of CIP by incomplete mineralization. Consequently, these results provided a novel insight into the application of UV-LED/PS treatment as a promising removal methodology for quinolones.

Validation of the Chinese Translation of the Spatial Hearing Questionnaire and Its Short Form.

PURPOSE: Few questionnaires address how to measure spatial hearing ability in complex listening situations. The purpose of the study was (a) to validate the Chinese translation of the Spatial Hearing Questionnaire (C-SHQ) among Chinese participants and (b) to provide a shortened version for the purpose of clinical screening. METHOD: This was a cross-sectional study. The C-SHQ was developed from the process of translation and back-translation of the original 24-item, English version (Tyler, Perreau, & Ji, 2009). The C-SHQ was administered to 146 patients at the Department of Otolaryngology Clinic of Sichuan Provincial People's Hospital between October 2013 and May 2014 at Sichuan, China. Exploratory factor analysis and reliability tests were performed for the full version, and confirmatory factor analysis was applied for the shortened version of the C-SHQ. RESULTS: The exploratory factor analysis revealed scores loaded on 3 similar factors compared with the original SHQ. The internal consistency reliability was high (Cronbach's α = 0.99). The confirmatory factor analysis indicated that a shortened version of 12 items is sufficient to measure spatial hearing abilities. CONCLUSIONS: The C-SHQ and its short form are both reliable and valid questionnaires, which are suitable for both research and clinical settings to measure spatial hearing ability in the Chinese population.

Sequential dynamic artificial neural network modeling of a full-scale coking wastewater treatment plant with fluidized bed reactors.

This study proposed a sequential modeling approach using an artificial neural network (ANN) to develop four independent models which were able to predict biotreatment effluent variables of a full-scale coking wastewater treatment plant (CWWTP). Suitable structure and transfer function of ANN were optimized by genetic algorithm. The sequential approach, which included two parts, an influent estimator and an effluent predictor, was used to develop dynamic models. The former parts of models estimated the variations of influent COD, volatile phenol, cyanide, and NH4 (+)-N. The later parts of models predicted effluent COD, volatile phenol, cyanide, and NH4 (+)-N using the estimated values and other parameters. The performance of these models was evaluated by statistical parameters (such as coefficient of determination (R (2) ), etc.). Obtained results indicated that the estimator developed dynamic models for influent COD (R (2) = 0.871), volatile phenol (R (2) = 0.904), cyanide (R (2) = 0.846), and NH4 (+)-N (R (2) = 0.777), while the predictor developed feasible models for effluent COD (R (2) = 0.852) and cyanide (R (2) = 0.844), with slightly worse models for effluent volatile phenol (R (2) = 0.752) and NH4 (+)-N (R (2) = 0.764). Thus, the proposed modeling processes can be used as a tool for the prediction of CWWTP performance.

The effect on localization of frequency-specific gain reduction schemes when matched and mismatched across ears.

OBJECTIVE: To evaluate the impact of non-adaptive matched and mismatched gain reduction schemes on localization in spatially-separated noise. DESIGN: Inspired by the function of commercial noise reduction algorithms, five frequency-specific gain reduction filter schemes were created, three for a modulated babble-noise and two for an unmodulated speech-shaped noise. Applying these schemes as both matched and mismatched conditions across ears, localization of five everyday sounds in noise was measured in a virtual environment using insert earphones. The performance in the reference scheme (no gain reduction in either ear) was measured as well. STUDY SAMPLE: Twenty-four adult bilateral hearing-aid users were enrolled in this study. RESULTS: One of the two mismatched gain reduction schemes for the unmodulated noise had a small but negative impact on localization compared to the reference scheme. For that scheme more high-frequency reduction was noted than for the other schemes. Matching gain reduction across ears restored the deteriorated localization performance. No localization performance differences were observed in the modulated babble-noise regardless of whether the scheme was matched or mismatched across ears. CONCLUSIONS: The impact of noise-induced gain reduction on localization in noise was trivial in the study regardless of whether gain reduction schemes were matched or not across ears.