Objetivos: Evaluar la asociación entre los hallazgos histológicos de la biopsia de glándula salival menor (BGSM) y los anticuerpos anti La (La/SS-B), anti Ro (Ro/SS-A), anticuerpos antinucleares (FAN) y factor reumatoideo (FR), y comparar el valor de estos como test diagnóstico con la BGSM considerada como patrón oro. Material y métodos: Se incluyeron pacientes con sospecha de síndrome de Sjögren primario (SSp) derivados para realización de BGSM. Se realizó medición de anticuerpos y se consideró BGSM positiva a los grados III y IV de la clasificación de Chisholm. Resultados: Se incluyeron 218 pacientes, 95% género femenino, con una mediana de edad de 54 años y de tiempo de evolución de los síntomas sicca de 12 meses. El 36% de las biopsias fueron positivas. El 33% de los pacientes presentaban anticuerpos anti Ro/SS-A anti La/SS-B positivos, 62% FAN positivo y el 31% FR positivo. Se encontró asociación estadísticamente significativa entre la BGSM y anti Ro/SS-A, anti La/SS-B, FAN y FR. El FAN resultó ser el anticuerpo más sensible (84% IC95%: 75-92), siendo los más específicos: anti Ro/ SS-A y/o anti La/ SS-B (78% IC95%: 71-85) y el FR (78% IC95%: 69-87). Conclusión: Ante la sospecha clínica de SSp, los anticuerpos anti Ro/ SS-A y anti La/ SS-B son de gran valor para arribar al diagnóstico, siendo la BGSM especialmente útil en los pacientes seronegativos. Los resultados también sugieren la utilidad del FAN y el FR para la clasificación de SSp (AU)
Objectives: To assess the association between histologic findings in the minor salivary gland biopsy (MSGB) and anti La (La/SS-B) y antiRo antibodies (Ro/SS-A), antinuclear antibodies (ANA) and Rheumatoid Factor (RF), and compare the value of the latter as diagnostic tests with MSGB, considered as the gold standard. Material and method: Patients with suspected Primary Sjögren Syndrome (PSS) referred for MSGB were included. Antibody measurements were performed. Grade III and IV biopsy results were considered positive. Results: Two hundred and eighteen (218) patients were included, 95% females, with a median age of 54 years and 12 months median duration of sicca symptoms. 36 of the biopsies were positive. 33% of patients had positive anti Ro/SS-Aanti La/SS-B antibodies, 62% had positive ANA, and 31% positive RF. Astatistically significant association was found between MSGB and anti Ro/SS-A anti La/SS-B, ANA and RF. ANA were the most sensitive antibodies (84%. 95% CI: 75- 92), and the most specific were: Anti Ro/ SS-A and/or anti La/ SS-B (78%. 95% CI: 71-85) and RF (78%. 95% CI: 69-87) (AU)
Humans , Sjogren's Syndrome/diagnosis , Keratoconjunctivitis Sicca/diagnosis , Salivary Glands, Minor/pathology , Antibody Formation , Biopsy/methods , Cross-Sectional Studies
OBJECTIVES: To assess the association between histologic findings in the minor salivary gland biopsy (MSGB) and anti La (La/SS-B)y antiRo antibodies (Ro/SS-A), antinuclear antibodies (ANA) and Rheumatoid Factor (RF),and compare the value of the latter as diagnostic tests with MSGB, considered as the gold standard. MATERIAL AND METHOD: Patients with suspected Primary Sjögren Syndrome (PSS) referred for MSGB were included. Antibody measurements were performed. Grade III and IV biopsy results were considered positive. RESULTS: Two hundred and eighteen (218) patients were included, 95% females, with a median age of 54 years and 12 months median duration of sicca symptoms. 36 of the biopsies were positive. 33% of patients had positive anti Ro/SS-A anti La/SS-B antibodies, 62% had positive ANA, and 31% positive RF. A statistically significant association was found between MSGB and anti Ro/SS-A anti La/SS-B, ANA and RF. ANA were the most sensitive antibodies (84%. 95% CI: 75- 92), and the most specific were: Anti Ro/ SS-A and/or anti La/ SS-B (78%. 95% CI: 71-85) and RF (78%. 95% CI: 69-87). CONCLUSION: On PSS clinical suspicion, anti Ro/ SS-A y anti La/ SS-B antibodies have a great value to achieve the diagnosis, with MSGB useful for diagnosis of seronegative patients. The results also suggest the importance of ANA and RF for PSS classification.
Antibodies, Antinuclear/blood , Rheumatoid Factor/blood , Salivary Glands, Minor/pathology , Sjogren's Syndrome/diagnosis , Adult , Biomarkers/blood , Biopsy , Cross-Sectional Studies , Female , Humans , Male , Middle Aged , Sensitivity and Specificity , Sjogren's Syndrome/blood , Sjogren's Syndrome/immunology , Sjogren's Syndrome/pathology
El hallazgo de anticuerpos específicos y datos histopatológicos son indispensables para llegar al diagnóstico de síndrome de Sjögren (SS). La biopsia de glándulas salivales menores (BGSM), si bien es un procedimiento sencillo, debe ser realizada en una institución a fin de evitar complicaciones. Objetivo: Estimar la frecuencia de complicaciones mediatas e inmediatas y el rédito de la técnica. Materiales y métodos: Se incluyeron los pacientes derivados al Hospital Rivadavia para realización de biopsia, entre octubre del 2007 y mayo del 2010. Los pacientes fueron citados a la semana y al mes del procedimiento para control de la lesión. Resultados: Frecuencia de complicaciones inmediatas (n = 186): 15 pacientes: 8,1%, IC del 95%, 4,7-13,2 (sangrado 7,5%, lipotimia 3,2%, hematomas 2,7%; no hubo accidentes). Complicaciones mediatas (n = 164): 16 pacientes: 9,75%, IC del 95%, 5,9-15,6 (dolor 7,32%, signos inflamatorios 3,66%, trastornos de sensibilidad 3,05%, granuloma 1,22%). No hubo casos de infecciones, ni dehiscencia del punto de sutura. Rédito microscópico: total 154 biopsias: se obtuvo tejido glandular en el 90,9%, IC del 95%, 85-95 (típica, sialoadenitis, infiltrado grado III y IV). Conclusiones: La BGSM presenta una baja frecuencia de complicaciones mediatas e inmediatas y un alto rédito en el estudio anatomo-patológico (AU)
Findings of specific antibodies and histopathology data are essential for the diagnosis of Sjögren syndrome (SS). Although the minor salivary gland biopsy (MSGB) is technically simple, it needs to be performed in a medical institution to avoid complications. Objective: To determine the frequency of complications and the usefulness of this technique. Materials and methods: Patients who underwent a minor salivary gland biopsy for a possible diagnosis of SS at Rivadavia Hospital between October 2007 and May 2010 where included. The patients were seen a week and a month after the procedure for follow up. Results: Frequency of acute complications (n = 186): 15 patients; 8.1%, 95%CI: 4.7-13.2 (Bleeding 7.5%, syncope 3.2%, hematoma 2.7%. No accidents occurred). Medium term complications (n = 164): 16 patients: 9.75%, 95%CI: 5.9-15.6 (pain 7.32%, inflammation 3.66%, sensitivity disorders 3.05%, granuloma 1.22%,). No infections or suture dehiscence occurred. Microscopic results: 154 biopsy reports were received: glandular 90.9%, 95%CI: 85-95 (typical, sialadenitis, grade III and IV infiltration). Conclusions: MSGB has very low frequency of medium term and acute complications and it has high usefulness (AU)
Humans , Male , Female , Middle Aged , Biopsy/methods , Biopsy , Salivary Gland Diseases/complications , Salivary Gland Diseases/diagnosis , Salivary Glands, Minor/cytology , Salivary Glands, Minor/pathology , Salivary Glands, Minor , Sjogren's Syndrome/complications , Immunosuppressive Agents/therapeutic use , Salivary Glands/microbiology , Salivary Glands/pathology , Prospective Studies , Longitudinal Studies , Methotrexate/therapeutic use , Azathioprine/therapeutic use
UNLABELLED: Findings of specific antibodies and histopathology data are essential for the diagnosis of Sjögren syndrome (SS). Although the minor salivary gland biopsy (MSGB) is technically simple, it needs to be performed in a medical institution to avoid complications. OBJECTIVE: To determine the frequency of complications and the usefulness of this technique. MATERIALS AND METHODS: Patients who underwent a minor salivary gland biopsy for a possible diagnosis of SS at Rivadavia Hospital between October 2007 and May 2010 where included. The patients were seen a week and a month after the procedure for follow up. RESULTS: Frequency of acute complications (n=186): 15 patients; 8.1%, 95% CI: 4.7-13.2 (Bleeding 7.5%, syncope 3.2%, hematoma 2.7%. No accidents occurred). Medium term complications (n=164): 16 patients: 9.75%, 95% CI: 5.9-15.6 (pain 7.32%, inflammation 3.66%, sensitivity disorders 3.05%, granuloma 1.22%,). No infections or suture dehiscence occurred. Microscopic results: 154 biopsy reports were received: glandular 90.9%, 95% CI: 85-95 (typical, sialadenitis, grade III and IV infiltration). CONCLUSIONS: MSGB has very low frequency of medium term and acute complications and it has high usefulness.
Biopsy , Salivary Glands, Minor/pathology , Sjogren's Syndrome/diagnosis , Autoantibodies/blood , Biopsy/adverse effects , Female , Hemorrhage/epidemiology , Hemorrhage/etiology , Humans , Immunosuppressive Agents/therapeutic use , Male , Middle Aged , Pain/epidemiology , Pain/etiology , Prospective Studies , Sensitivity and Specificity , Sjogren's Syndrome/blood , Sjogren's Syndrome/complications , Sjogren's Syndrome/drug therapy , Sjogren's Syndrome/etiology , Sjogren's Syndrome/pathology , Syncope/epidemiology , Syncope/etiology
