ABSTRACT
OBJECTIVE: To investigate the correlation between matrix metalloproteinase-2 (MMP-2) gene polymorphism and cataract. PATIENTS AND METHODS: 104 cataract patients and 100 healthy subjects were enrolled and assigned to the observation group and control group, respectively. General clinical data of the enrolled subjects were collected. The inflammatory factors were detected, and the rs243865 polymorphism of MMP-2 gene was detected using the TaqMan-MGB probe. RESULTS: The levels of interleukin-6 (IL-6), IL-1ß, C-reactive protein (CRP), and tumor necrosis factor-1α (TNF-1α) in the observation group were higher than those in the control group (p<0.05). There were significant differences in the genotype and allele distribution frequency between the two groups (p<0.05). In the genetic model analysis, the additive model was remarkably different between the two groups (p<0.05). However, the recessive model and dominant model were not different between the two groups (p>0.05). CONCLUSIONS: Cataract is correlated with inflammatory factors, and the rs243865 polymorphism of MMP-2 gene has a correlation with the incidence of cataract.
Subject(s)
Cataract/genetics , Genetic Predisposition to Disease , Matrix Metalloproteinase 2/genetics , Polymorphism, Single Nucleotide , Aged , C-Reactive Protein/administration & dosage , Case-Control Studies , Cataract/epidemiology , Cataract/immunology , Female , Gene Frequency , Humans , Incidence , Interleukin-1beta/blood , Interleukin-6/blood , Male , Tumor Necrosis Factor-alpha/bloodABSTRACT
MicroRNA-32 (miR-32) has been shown to be dysregulated in some human malignancies and this has been found to be correlated with tumor progression. However, its role in uveal melanoma formation and progression remains largely unknown. Thus, the aim of this study was to explore the expression and function of miR-32 in human uveal melanoma. Using quantitative reverse transcription-polymerase chain reaction, we detected miR-32 expression in uveal melanoma tumor tissues and cell lines. The effects of miR-32 on the biological behavior of uveal melanoma cells were also investigated. Finally, the potential regulatory function of miR-32 on EZH2 expression was confirmed. miR-32 expression levels were significantly downregulated in uveal melanoma samples and cell lines (both P < 0.01). Ectopic expression of miR-32 could inhibit uveal melanoma cell proliferation, migration, and invasion, and promote cell apoptosis in vitro. Further, EZH2 was confirmed as a direct target of miR-32 by using the luciferase reporter assay. These findings indicate that miR-32 may function as a novel tumor suppressor in uveal melanoma and could be a potential therapeutic target for this disease.