ABSTRACT
An 11-year-old female goat had invasive and metastatic endometrial adenocarcinoma in the uterus. There was a notable proliferation of endometrial epithelial cells in a tubular growth pattern, with a desmoplastic response. The endometrial epithelial tumor cells metastasized to the kidney, liver and lung. In contrast to the primary and metastatic tumor cells, pleomorphic tumor cells with a choriocarcinoma-like growth pattern infiltrated the mesometrium. Cell proliferation activity was high in both types of tumor cells. Both types of tumor cells expressed cytokeratins AE1/AE3, 7 and CAM5.2; choriocarcinomatous cells also had positive immunoreactions to human chorionic gonadotropin, human placental alkaline phosphatase and α-inhibin. The present case was diagnosed as endometrial adenocarcinoma with choriocarcinomatous differentiation.
Subject(s)
Adenocarcinoma/veterinary , Choriocarcinoma/veterinary , Endometrial Neoplasms/veterinary , Goat Diseases/pathology , Uterine Neoplasms/veterinary , Adenocarcinoma/pathology , Animals , Choriocarcinoma/pathology , Endometrial Neoplasms/pathology , Female , Goat Diseases/diagnosis , Goats , Uterine Neoplasms/pathologyABSTRACT
To investigate the protective effect of bilberry extracts (BBE) and enzymatically modified isoquercitrin (EMIQ) on the hepatocarcinogenic process involving oxidative stress responses, we used a two-stage hepatocarcinogenesis model in N-diethylnitrosamine-initiated and piperonyl butoxide (PBO)-promoted rats. We examined the modifying effect of co-administration with BBE or EMIQ on the liver tissue environment including oxidative stress responses, cell proliferation and apoptosis, and phosphatase and tensin homolog (PTEN)/Akt and transforming growth factor (TGF)-ß/Smad signalings on the induction mechanism of preneoplastic lesions during early stages of hepatocellular tumor promotion. PBO increased the numbers and area of glutathione S-transferase placental form (GST-P)(+) liver cell foci and the numbers of Ki-67(+) proliferating cells within GST-P(+) foci. Co-administration of BBE or EMIQ suppressed these effects with the reductions of GST-P(+) foci (area) to 48.9-49.4% and Ki-67(+) cells to 55.5-61.4% of the PBO-promoted cases. Neither BBE nor EMIQ decreased microsomal reactive oxygen species induced by PBO. However, only EMIQ suppressed the level of thiobarbituric acid-reactive substances to 78.4% of the PBO-promoted cases. PBO increased the incidences of phospho-PTEN(-) foci, phospho-Akt substrate(+) foci, phospho-Smad3(-) foci and Smad4(-) foci in GST-P(+) foci. Both BBE and EMIQ decreased the incidences of phospho-PTEN(-) foci in GST-P(+) foci to 59.8-72.2% and Smad4(-) foci to 62.4-71.5% of the PBO-promoted cases, and BBE also suppressed the incidence of phospho-Akt substrate(+) foci in GST-P(+) foci to 75.2-75.7% of the PBO-promoted cases. These results suggest that PBO-induced tumor promotion involves facilitation of PTEN/Akt and disruptive TGF-ß/Smad signalings without relation to oxidative stress responses, but this promotion was suppressed by co-treatment with BBE or EMIQ through suppression of cell proliferation activity of preneoplastic liver cells.
Subject(s)
Anticarcinogenic Agents/therapeutic use , Liver Neoplasms, Experimental/prevention & control , Piperonyl Butoxide/toxicity , Plant Extracts/therapeutic use , Precancerous Conditions/prevention & control , Quercetin/analogs & derivatives , Vaccinium myrtillus/chemistry , Animals , Anticarcinogenic Agents/administration & dosage , Apoptosis/drug effects , Cell Proliferation/drug effects , Cocarcinogenesis , Diethylnitrosamine/toxicity , Glycosylation , Liver Neoplasms, Experimental/chemically induced , Liver Neoplasms, Experimental/metabolism , Liver Neoplasms, Experimental/pathology , Male , Oxidative Stress/drug effects , Plant Extracts/administration & dosage , Plant Extracts/isolation & purification , Precancerous Conditions/chemically induced , Precancerous Conditions/metabolism , Precancerous Conditions/pathology , Quercetin/administration & dosage , Quercetin/isolation & purification , Quercetin/therapeutic use , Rats, Inbred F344ABSTRACT
Three spherical opaque-white tumor nodules were found in close proximity to each other in the liver of a breeding sow, postslaughter, at a veterinary food inspection. The tumor nodules were circumscribed and histologically consisted of discrete hepatocellular and cholangiocellular nests, in association with polygonal-to-oval-shaped cells with slight cellular atypia. Immunohistochemically, all cellular components were negative for carcinoembryonic antigen, but positive for p53. Both cholangiocytes and oval-shaped cells were immunoreactive to anti-cytokeratin antibodies AE1/AE3 and MNF116. In addition, cholangiocytes were exclusively immunoreactive to anti-cytokeratin antibody CAM5.2, and hepatocytes were positive for MNF116 and hepatocyte paraffin 1. All neoplastic cells were positive for the hepatic progenitor cell markers, α-1-fetoprotein, sal-like protein 4, and epithelial cell adhesion molecule. From these results, the present case was diagnosed as hepatocholangiocellular adenoma, arising from epithelial cells of the canals of Hering.
ABSTRACT
N-Methyl-N-nitrosourea (MNU) is an alkylating agent having antiproliferative cytotoxity targeting the neural stem/progenitor cells to cause microcephaly by maternal exposure. This study investigated the effect of transient exposure to MNU on the process of hippocampal neurogenesis in later life using mice. Pregnant mice received a single injection of MNU at 0, 5 and 10 mg/kg body weight, intraperitoneally on gestational day 14, and their offspring were examined on postnatal day (PND) 21 and PND 77. On PND 21, offspring displayed microcephaly and hippocampal formation hypoplasia at 10 mg/kg, decrease of doublecortin (Dcx)(+) cells in the dentate subgranular zone from 5mg/kg, and decrease of TUNEL(+) apoptotic cells and increase of transcript expression of anti-apoptotic Bcl-2 at 10 mg/kg in the dentate gyrus. In the dentate hilus, numbers of reelin(+) or parvalbumin (Pvalb)(+) interneurons or neuron-specific nuclear protein(+) neurons increased at 10 mg/kg. Microcephaly and hippocampal formation hypoplasia continued through PND 77 at 10 mg/kg. Thus, apart from the massive cell killing at the migratory stream causing microcephaly, MNU may decrease Dcx(+) cells reflecting disruption of the differentiation process of late-stage neuronal progenitors and immature granule cells through defective molecular functions by gene mutations. Increase of reelin(+) and Pvalb(+) cells may reflect the disruption of neurogenesis and following neuronal migration. All of the granule cell lineage and interneuron changes disappeared at the adult stage on PND 77 suggesting that MNU mainly targets transient populations of highly proliferative progenitor cells but hardly affects their stem cells having self-renewal ability.
Subject(s)
Alkylating Agents/toxicity , Hippocampus/drug effects , Methylnitrosourea/toxicity , Microcephaly/chemically induced , Neural Stem Cells/drug effects , Neurogenesis/drug effects , Prenatal Exposure Delayed Effects , Animals , Apoptosis/drug effects , Cell Adhesion Molecules, Neuronal/genetics , Cell Adhesion Molecules, Neuronal/metabolism , Cell Lineage , Cell Proliferation/drug effects , Doublecortin Domain Proteins , Doublecortin Protein , Extracellular Matrix Proteins/genetics , Extracellular Matrix Proteins/metabolism , Female , Gestational Age , Hippocampus/metabolism , Hippocampus/pathology , Hippocampus/physiopathology , Injections, Intraperitoneal , Interneurons/drug effects , Interneurons/metabolism , Interneurons/pathology , Male , Maternal Exposure , Mice , Mice, Inbred ICR , Microcephaly/genetics , Microcephaly/metabolism , Microcephaly/pathology , Microcephaly/physiopathology , Microtubule-Associated Proteins/metabolism , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/metabolism , Neural Stem Cells/metabolism , Neural Stem Cells/pathology , Neuropeptides/metabolism , Parvalbumins/genetics , Parvalbumins/metabolism , Pregnancy , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , Reelin Protein , Serine Endopeptidases/genetics , Serine Endopeptidases/metabolism , Time FactorsABSTRACT
Indole-3-carbinol (I3C) and phenobarbital (PB) are cytochrome P450 (CYP) 1A and CYP2B inducers, respectively, and have liver tumor-promoting effects in rats. In this study, we investigated the modifying effects on tumor promotion by I3C and PB co-administration. Six-week-old male F344 rats received a single intraperitoneal injection of N-diethylnitrosamine for initiation treatment. Two weeks after the initiation, rats were given no tumor-promoting agents (DEN alone), I3C (2,500 or 5,000 ppm in diet), PB (60 or 120 ppm in drinking water), or 2,500 ppm I3C + 60 ppm PB for 6 weeks. One week after the I3C/PB treatments, all animals underwent a two-thirds partial hepatectomy. The number and area of liver cell foci positive for glutathione S-transferase placental form (GST-P(+) foci) were not significantly fluctuated in the PB+I3C group in the isoadditive statistical model. On the contrary, the mRNA levels of Cyp2b1/2 and Nqo1 were suppressed and enhanced, respectively, in the PB+I3C group in the isoadditive model, but there was no enhancement in the microsomal reactive oxygen species (ROS) production, thiobarbituric acid-reactive substance levels, and Ki-67(+) cell ratio in this group. The results suggest that the co-administration of I3C and PB causes no modifying effects in liver tumor promotion in rats.
Subject(s)
Anticarcinogenic Agents/pharmacology , Indoles/pharmacology , Liver Neoplasms/chemically induced , Liver Neoplasms/pathology , Phenobarbital/pharmacology , Animals , Anticarcinogenic Agents/administration & dosage , Carcinogens , Cytochrome P-450 CYP2B1/metabolism , Cytochrome P-450 Enzyme System/metabolism , Diethylnitrosamine , Enzyme Induction/drug effects , Glutathione Transferase/metabolism , Indoles/administration & dosage , Male , NAD(P)H Dehydrogenase (Quinone)/metabolism , Phenobarbital/administration & dosage , Rats , Rats, Inbred F344ABSTRACT
To investigate the protective effect of α-lipoic acid (a-LA) on the hepatocarcinogenic process promoted by thioacetamide (TAA), we used a two-stage liver carcinogenesis model in N-diethylnitrosamine (DEN)-initiated and TAA-promoted rats. We examined the modifying effect of co-administered a-LA on the liver tissue environment surrounding preneoplastic hepatocellular lesions, with particular focus on hepatic macrophages and the mechanism behind the decrease in apoptosis of cells surrounding preneoplastic hepatocellular lesions during the early stages of hepatocellular tumor promotion. TAA increased the number and area of glutathione S-transferase placental form (GST-P)(+) liver cell foci and the numbers of proliferating and apoptotic cells in the liver. Co-administration with a-LA suppressed these effects. TAA also increased the numbers of ED2(+), cyclooxygenase-2(+), and heme oxygenase-1(+) hepatic macrophages as well as the number of CD3(+) lymphocytes. These effects were also suppressed by a-LA. Transcript levels of some inflammation-related genes were upregulated by TAA and downregulated by a-LA in real-time RT-PCR analysis. Outside the GST-P(+) foci, a-LA reduced the numbers of apoptotic cells, active caspase-8(+) cells and death receptor (DR)-5(+) cells. These results suggest that hepatic macrophages producing proinflammatory factors may be activated in TAA-induced tumor promotion. a-LA may suppress tumor-promoting activity by suppressing the activation of these macrophages and the subsequent inflammatory responses. Furthermore, a-LA may suppress tumor-promoting activity by suppressing the DR5-mediated extrinsic pathway of apoptosis and the subsequent regeneration of liver cells outside GST-P(+) foci.