ABSTRACT
In response to alkaline ambient pH, the Aspergillus nidulans PacC transcription factor mediating pH regulation of gene expression is activated by proteolytic removal of a negative-acting C-terminal domain. We demonstrate interactions involving the approximately 150 C-terminal PacC residues and two regions located immediately downstream of the DNA binding domain. Our data indicate two full-length PacC conformations whose relative amounts depend upon ambient pH: one 'open' and accessible for processing, the other 'closed' and inaccessible. The location of essential determinants for proteolytic processing within the two more upstream interacting regions probably explains why the interactions prevent processing, whereas the direct involvement of the C-terminal region in processing-preventing interactions explains why C-terminal truncating mutations result in alkalinity mimicry and pH-independent processing. A mutant PacC deficient in pH signal response and consequent processing behaves as though locked in the 'closed' form. Single-residue substitutions, obtained as mutations bypassing the need for pH signal transduction, identify crucial residues in each of the three interactive regions and overcome the processing deficiency in the 'permanently closed' mutant.
Subject(s)
Fungal Proteins , Transcription Factors/metabolism , Amino Acid Sequence , Amino Acid Substitution , Aspergillus nidulans/genetics , Aspergillus nidulans/metabolism , Binding Sites/genetics , Endopeptidases/metabolism , Gene Expression Regulation , Genes, Fungal , Hydrogen-Ion Concentration , Models, Biological , Molecular Sequence Data , Point Mutation , Protein Conformation , Protein Processing, Post-Translational , Protein Structure, Tertiary , Sequence Deletion , Signal Transduction , Transcription Factors/chemistry , Transcription Factors/genetics , Two-Hybrid System Techniques , Zinc Fingers/geneticsABSTRACT
Completing the molecular analysis of the six pal genes of the ambient pH signal transduction pathway in Aspergillus nidulans, we report the characterization of palC and palH. The derived translation product of palH contains 760 amino acids with prediction of seven transmembrane domains in its N-terminal moiety. Remarkably, a palH frameshift mutant lacking just over half the PalH protein, including almost all of the long hydrophilic region C-terminal to the transmembrane domains, retains some PalH function. The palC-derived translation product contains 507 amino acids, and the null phenotype of a frameshift mutation indicates that at least one of the C-terminal 142 residues is essential for function. Uniquely among the A. nidulans pH-signalling pal genes, palC appears to have no Saccharomyces cerevisiae homologue, although it does have a Neurospora crassa expressed sequence tag homologue. In agreement with findings for the palA, palB and palI genes of this signalling pathway, levels of the palC and palH mRNAs do not appear to be pH regulated.
Subject(s)
Aspergillus nidulans/genetics , Aspergillus nidulans/metabolism , Fungal Proteins/genetics , Fungal Proteins/metabolism , Signal Transduction , Amino Acid Sequence , Base Sequence , Cloning, Molecular , Frameshift Mutation , Hydrogen-Ion Concentration , Molecular Sequence Data , Sequence Homology, Amino Acid , Transcription, GeneticABSTRACT
We describe the cloning, sequence and expression of gabA, encoding the gamma-amino-n-butyrate (GABA) permease of the fungus Aspergillus nidulans. Sequence changes were determined for three up-promoter (gabI ) and six gabA loss-of-function mutations. The predicted protein contains 517 residues and shows 30.3% overall identity with a putative GABA permease of Arabidopsis thaliana, 29.6% identity with the yeast choline transporter and 23.4% identity with the yeast UGA4 GABA permease. Structural predictions favour 11-12 transmembrane domains. Comparison of the genomic and cDNA sequences shows the presence of 19 introns, an unusually large number of introns for, we believe, any fungal gene. In agreement with the wealth of genetic data available, transcript level analyses demonstrate that gabA is subject to carbon catabolite and nitrogen metabolite repression, omega-amino acid induction and regulation in response to ambient pH (being acid-expressed). In agreement with this, we report consensus binding sites 5' to the coding region, six each for CreA and AREA and one for PacC, the transcription factors mediating carbon catabolite and nitrogen metabolite repression and response to ambient pH respectively.
Subject(s)
Aspergillus nidulans/genetics , Membrane Transport Proteins/genetics , Organic Anion Transporters , Amino Acid Sequence , Base Sequence , Cloning, Molecular , Exons , Fungal Proteins/genetics , Fungal Proteins/metabolism , Gene Expression Regulation, Fungal , Membrane Transport Proteins/metabolism , Molecular Sequence Data , Mutation , Promoter Regions, Genetic , Sequence Analysis , Sequence Homology, Amino Acid , Transcription, GeneticABSTRACT
The Aspergillus nidulans transcription factor PacC, which mediates pH regulation, is proteolytically processed to a functional form in response to ambient alkaline pH. The full-length PacC form is unstable in the presence of an operational pH signal transduction pathway, due to processing to the relatively stable short functional form. We have characterized and used an extensive collection of pacC mutations, including a novel class of "neutrality-mimicking" pacC mutations having aspects of both acidity- and alkalinity-mimicking phenotypes, to investigate a number of important features of PacC processing. Analysis of mutant proteins lacking the major translation initiation residue or truncated at various distances from the C terminus showed that PacC processing does not remove N-terminal residues, indicated that processing yields slightly heterogeneous products, and delimited the most upstream processing site to residues approximately 252 to 254. Faithful processing of three mutant proteins having deletions of a region including the predicted processing site(s) and of a fourth having 55 frameshifted residues following residue 238 indicated that specificity determinants reside at sequences or structural features located upstream of residue 235. Thus, the PacC protease cuts a peptide bond(s) remote from these determinants, possibly thereby resembling type I endonucleases. Downstream of the cleavage site, residues 407 to 678 are not essential for processing, but truncation at or before residue 333 largely prevents it. Ambient pH apparently regulates the accessibility of PacC to proteolytic processing. Alkalinity-mimicking mutations L259R, L266F, and L340S favor the protease-accessible conformation, whereas a protein with residues 465 to 540 deleted retains a protease-inaccessible conformation, leading to acidity mimicry. Finally, not only does processing constitute a crucial form of modulation for PacC, but there is evidence for its conservation during fungal evolution. Transgenic expression of a truncated PacC protein, which was processed in a pH-independent manner, showed that appropriate processing can occur in Saccharomyces cerevisiae.
Subject(s)
Aspergillus nidulans/metabolism , Fungal Proteins/metabolism , Transcription Factors/metabolism , Amino Acid Sequence , Aspergillus nidulans/genetics , Binding Sites , Fungal Proteins/chemistry , Fungal Proteins/genetics , Hydrogen-Ion Concentration , Molecular Sequence Data , Mutation , Plasmids/genetics , Protein Processing, Post-Translational , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Signal Transduction , Transcription Factors/chemistry , Transcription Factors/genetics , Transformation, GeneticABSTRACT
The zinc finger regions of the Aspergillus nidulans PacC transcription factor, mediating regulation of gene expression by ambient pH, and the Saccharomyces cerevisiae Rim1p transcription factor, mediating control of meiosis and invasiveness, are homologous and both transcription factors undergo proteolytic processing of the C-terminus for conversion to the functional form. In both cases, functioning of a signal transduction pathway involving several gene products is a necessary prerequisite for processing. We now show that the Aspergillus PalI pH signal transduction component is homologous to the Saccharomyces Rim9p meiotic signal transduction component throughout a region containing four hydrophobic, putative membrane-spanning segments. This suggests that PalI might be a membrane sensor for ambient pH. Deletion of the palI gene established that the less extreme phenotype of palI mutations compared with mutations in the other five genes of the pH signalling pathway is a general feature of palI mutations.
Subject(s)
Aspergillus nidulans/genetics , Fungal Proteins/genetics , Fungal Proteins/metabolism , Meiosis , Membrane Proteins , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Signal Transduction , Amino Acid Sequence , Aspergillus nidulans/metabolism , Base Sequence , Blotting, Northern , Cloning, Molecular , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Deoxyribonucleases, Type II Site-Specific/genetics , Deoxyribonucleases, Type II Site-Specific/metabolism , Hydrogen-Ion Concentration , Molecular Sequence Data , Mutation , Repressor Proteins , Saccharomyces cerevisiae/metabolism , Sequence Homology, Amino Acid , Transcription Factors/genetics , Transcription Factors/metabolismABSTRACT
The three zinc fingers of PacC, the transcription factor mediating pH regulation in Aspergillus nidulans, are necessary and sufficient to recognise specifically the target ipnA2 site. Missing nucleoside footprints confirmed the core target (double-stranded) hexanucleotide 5'-GCCAAG-3'. Any base substitution resulted in substantial or complete loss of binding, excepting A5 (partially replaceable by G). A T preceding the hexanucleotide enhanced binding. Interference footprinting indicates that the four Gs and A4 participate in specific contacts and that five pyrimidines are essential for binding. The size of the target sequence and the amino acid sequence of finger 1 suggested that its probe helix would not participate in base-specific contacts. Using site-directed mutagenesis and analogy to GLI, we propose that finger 1 crucially interacts with finger 2, a pair of conserved Trp residues in the Cys knuckles contacting hydrophobically. Finger 2 would also participate in extensive base contacts with the 5' moiety of the hexanucleotide. The specificity mutation Lys159Gln shows that finger 3 binds the 3' moiety of the hexanucleotide. Replacement of residues in positions +3 (His128Asn) and +2 (Gln155Lys) of the reading helices of fingers 2 and 3, respectively, prevented binding. Our biochemical and molecular data plus modelling using previously determined zinc finger-DNA complexes, predict specific contacts of fingers 2 and 3 to ipnA2. Our data indicate compact organisation of the PacC-ipnA2 complex (with nearly every base involved in specific contacts), illustrate the binding versatility of zinc finger domains and should facilitate analysis of other PacC family members, including Saccharomyces cerevisiae RIM1.
Subject(s)
Aspergillus nidulans/chemistry , DNA/metabolism , Fungal Proteins , Transcription Factors/chemistry , Transcription Factors/metabolism , Zinc Fingers , Amino Acid Sequence , Base Composition , Binding Sites , DNA Footprinting , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Oligonucleotides , Protein Conformation , Transcription Factors/geneticsSubject(s)
Aspergillus/physiology , Gene Expression Regulation, Fungal , Promoter Regions, Genetic , Acid Phosphatase/biosynthesis , Acid Phosphatase/genetics , Amino Acid Sequence , Aspergillus/genetics , Base Sequence , Culture Media , Hydrogen-Ion Concentration , Metalloendopeptidases/biosynthesis , Metalloendopeptidases/genetics , Molecular Sequence DataABSTRACT
A wide domain regulatory gene implicated in modulating gene expression in response to ambient pH has been cloned and sequenced from the industrially useful filamentous fungus Aspergillus niger. This gene, pacC, is able to restore a pacC+ phenotype to A. nidulans pacCc11 and pacCc14 mutants with respect to extent of conidiation, conidial pigment intensity and acid phosphatase regulation. The pacC gene of A. niger comprises three exons, encodes a three-zinc-finger protein of 677 amino acids, and shows pH-dependent regulation of expression: mRNA levels are elevated under alkaline conditions and considerably reduced under acidic conditions. The occurrence of PacC consensus binding targets within the sequences upstream of pacC may indicate autoregulation.
Subject(s)
Aspergillus niger/genetics , Genes, Fungal , Genes, Regulator , Transcription Factors/chemistry , Transcription Factors/genetics , Acid Phosphatase/metabolism , Amino Acid Sequence , Aspergillus niger/chemistry , Aspergillus niger/metabolism , Base Sequence , Blotting, Northern , Cloning, Molecular , Electrophoresis, Polyacrylamide Gel , Fungal Proteins/chemistry , Fungal Proteins/genetics , Gene Expression Regulation, Fungal/genetics , Hydrogen-Ion Concentration , Molecular Sequence Data , Phenotype , Protein Biosynthesis/genetics , Restriction Mapping , Sequence Alignment , Zinc Fingers/geneticsABSTRACT
Extremes of pH are an occupational hazard for many microorganisms. In addition to efficient pH homeostasis, survival effectively requires a regulatory system tailoring the syntheses of molecules functioning beyond the cell boundaries (permeases, secreted enzymes, and exported metabolites) to the pH of the growth environment. Our previous work established that the zinc finger PacC transcription factor mediates such pH regulation in the fungus Aspergillus nidulans in response to a signal provided by the products of the six pal genes at alkaline ambient pH. In the presence of this signal, PacC becomes functional, activating transcription of genes expressed at alkaline pH and preventing transcription of genes expressed at acidic pH. Here we detect two forms of PacC in extracts, both forming specific retardation complexes with a PacC-binding site. Under acidic growth conditions or in acidity-mimicking pal mutants (defective in ambient pH signal transduction), the full-length form of PacC predominates. Under alkaline growth conditions or in alkalinity-mimicking pacCc mutants (independent of the ambient pH signal), a proteolysed version containing the amino-terminal approximately 40% of the protein predominates. This specifically cleaved shorter version is clearly functional, both as an activator for alkaline-expressed genes and as a repressor for acid-expressed genes, but the full-length form of PacC must be inactive. Thus, PacC proteolysis is an essential and pH-sensitive step in the regulation of gene expression by ambient pH. Carboxy-terminal truncations, resulting in a gain-of-function (pacCc) phenotype, bypass the requirement for the pal signal transduction pathway for conversion of the full-length to the proteolyzed functional form.
Subject(s)
Aspergillus nidulans/genetics , Aspergillus nidulans/metabolism , Fungal Proteins , Gene Expression Regulation, Fungal , Genes, Fungal , Hydrogen-Ion Concentration , Transcription Factors/metabolism , Aspergillus nidulans/growth & development , Base Sequence , Binding Sites , Blotting, Western , Consensus Sequence , Endopeptidases/metabolism , Genotype , Mutagenesis, Site-Directed , Protein Biosynthesis , Recombinant Fusion Proteins/metabolism , Transcription Factors/genetics , Transcription, Genetic , Zinc FingersABSTRACT
The pH regulation of gene expression in Aspergillus nidulans is mediated by pacC, whose 678 residue-derived protein contains three putative Cys2His2 zinc fingers. Ten pacCc mutations mimicking growth at alkaline pH remove between 100 and 214 C-terminal residues, including a highly acidic region containing an acidic glutamine repeat. Nine pacC+/- mutations mimicking acidic growth conditions remove between 299 and 505 C-terminal residues. Deletion of the entire pacC coding region mimics acidity but leads additionally to poor growth and conidiation. A PacC fusion protein binds DNA with the core consensus GCCARG. At alkaline ambient pH, PacC activates transcription of alkaline-expressed genes (including pacC itself) and represses transcription of acid-expressed genes. pacCc mutations obviate the need for pH signal transduction.
Subject(s)
Acid-Base Equilibrium , Aspergillus nidulans/genetics , DNA-Binding Proteins/metabolism , Fungal Proteins/genetics , Transcription Factors/physiology , Amino Acid Sequence , Base Sequence , Binding Sites , Cloning, Molecular , Epistasis, Genetic , Gene Expression Regulation, Fungal , Molecular Sequence Data , Mutagenesis, Site-Directed , Promoter Regions, Genetic , RNA, Messenger/genetics , Sequence Alignment , Sequence Homology, Amino Acid , Structure-Activity Relationship , Zinc FingersABSTRACT
Two new genes, palH and palI, where mutations mimic the effects of acidic growth pH have been identified in Aspergillus nidulans. A palH mutation is phenotypically indistinguishable from mutations in the palA, palB, palC, and palF genes, whereas palI mutations differ only in that they allow some growth at pH 8. Mutations in palA, B, C, F, and H are epistatic to a palI mutation and the significance of this epistasis is discussed. Additionally, palE and palB mutations have been shown to be allelic. Thus, a total of six genes where mutations mimic acidic growth conditions has been identified.
Subject(s)
Aspergillus nidulans/genetics , Chemoreceptor Cells/physiology , Genes, Fungal , Alleles , Chromosome Mapping , Hydrogen-Ion Concentration , MutationABSTRACT
Transcription of the ipnA gene encoding isopenicillin N synthetase, an enzyme of secondary metabolism, is under the control of the pH regulatory system in the fungus Aspergillus nidulans. External alkaline pH or mutations in pacC, the wide domain regulatory gene which mediates pH regulation, override carbon regulation of ipnA transcript levels, resulting in elevation of the levels of this message in sucrose broth. Strains carrying these mutations, which mimic growth at alkaline pH, produce higher levels of penicillins when grown in sucrose broth compared with the wild type strain grown under carbon derepressing conditions. ipnA transcription is regulated by carbon (C) source, but extreme mutations in creA (the regulatory gene mediating carbon catabolite repression) only slightly increase repressed transcript levels. Precise deletion of the only in vitro CreA binding site present in a region of the ipnA promoter involved in carbon regulation has no effect on ipnA expression. The levels of ipnA transcript in broths with acetate or glycerol as principal C sources are inconsistent with direct or indirect creA-mediated transcriptional control of the gene. We conclude that a second, creA-independent mechanism of carbon repression controls expression of this gene. All derepressing C sources tested result in alkalinization of the growth media. In contrast, all repressing C sources result in external acidification. Neither acidic external pH nor pal mutations, mimicking the effects of growth at acid pH, prevent carbon derepression, providing strong support for independent regulatory mechanisms, one mediating carbon regulation (via thus far unidentified genes) and another mediating pH regulation (through the pacC-encoded transcriptional regulator). External pH measurements suggest that these two independent forms of regulation normally act in concert. We propose that external alkalinity represents a physiological signal which triggers penicillin biosynthesis.
Subject(s)
Aspergillus nidulans/enzymology , Oxidoreductases/genetics , Penicillins/biosynthesis , Acetates/metabolism , Aspergillus nidulans/genetics , Base Sequence , Carbon/metabolism , DNA, Fungal , Fungal Proteins/metabolism , Gene Expression Regulation, Enzymologic , Genes, Fungal , Genes, Regulator , Glycerol/metabolism , Hydrogen-Ion Concentration , Kinetics , Molecular Sequence Data , Mutation , Oxidoreductases/metabolism , Promoter Regions, Genetic , Repressor Proteins/metabolism , Transcription, GeneticABSTRACT
We have cloned an 11-kbp segment of the genomic DNA of Aspergillus nidulans which complements mutations in nirA, the pathway-specific regulatory gene of the nitrate assimilation pathway. Gene disruption in the corresponding region of the nuclear DNA leads to a phenotype and a gene complementation pattern indistinguishable from that observed in known noninducible nirA mutants. Transformation studies with subclones of the 11-kbp genomic segment showed that a nonreverting null mutation nirA87, maps to a 1.5-kbp stretch within that segment. These data confirm that the cloned segment contains the nirA gene. The gene is completely encompassed in the 11-kbp genomic segment, as a plasmid carrying the corresponding insert gives rise to multicopy transformants exhibiting better growth than wild type on nitrate or nitrite as the sole nitrogen source. Southern and genetic analyses of transformants obtained with various plasmid subclones established a gene size of at most 5.9 kbp. Northern (RNA) hybridization experiments revealed a 4-kb nirA transcript which is barely visible in the wild type but clearly seen in a transformant carrying about 10 gene copies. In both strains, nirA mRNA is synthesized constitutively. Upstream of nirA, a neighboring transcript about 2.8 kbp in length which is transcribed from the opposite strand with respect to nirA was localized. The transcript levels of niaD and niiA, encoding the nitrate and nitrite reductase core proteins, respectively, were investigated in nirA mutants and a nirA multicopy transformant. The results show that the nirA product regulates the transcript steady-state level of these structural genes and that it is a limiting factor for their expression.
Subject(s)
Aspergillus nidulans/genetics , Genes, Fungal , Genes, Regulator , Nitrates/metabolism , Aspergillus nidulans/metabolism , Cloning, Molecular/methods , Crosses, Genetic , DNA, Fungal/genetics , DNA, Fungal/isolation & purification , Genetic Linkage , Genomic Library , Genotype , Plasmids , Restriction Mapping , Transformation, GeneticABSTRACT
As shown by both bioassay and high-performance liquid chromatographic (HPLC) analysis, penicillin G production by Aspergillus nidulans is subject to regulation by the pH of the growth medium. Penicillin titres were highest at alkaline pH and in strains carrying mutations in the regulatory gene pacC which mimics the effects of growth at alkaline pH. They were lowest at acid pH and in strains carrying mutations in the palA, palB, palC, palE or palF genes which mimic the effects of growth at acid pH.
Subject(s)
Aspergillus nidulans/physiology , Penicillins/biosynthesis , Aspergillus nidulans/genetics , Aspergillus nidulans/growth & development , Chromatography, High Pressure Liquid , Fermentation , Genotype , Hydrogen-Ion Concentration , Penicillin G/analysisABSTRACT
We describe examples of wA gene inactivation (resulting in white conidiospores) obtained during transformation of Aspergillus nidulans. One wA- transformant was obtained by transformation with a prn+ plasmid of a strain with green conidia (wA+) which was unable to catabolize L-proline (prn-). This transformant contains a very large number of plasmid copies integrated at a single site inseparable from the wA locus. Passage of this transformant through the sexual cycle generated a variety of novel phenotypes for L-proline utilization, the number and frequency of which depended upon the cleistothecium from which the progeny were obtained, suggesting that the altered phenotypes were due to premeiotic events. The most extreme phenotype was severe hypersensitivity to L-proline. Hypersensitive progeny had a much reduced number of integrated plasmid copies enabling us to identify and clone putative prn-wA fusion sequences and subsequently retrieve wA sequences from a wild-type gene library. One of the wild-type clones overlapped the different sites of the insertional mutations in two wA- transformants and complemented the wA3 allele. Sequences within this clone hybridized to a transcript that was developmentally regulated in the wild type and absent in a number of mutants defective in conidiospore development. A reiterated sequence was also found in the region of the wA gene.
Subject(s)
Aspergillus nidulans/genetics , DNA Transposable Elements , Gene Expression Regulation, Fungal , Genes, Fungal , Transformation, Genetic , Aspergillus nidulans/growth & development , Blotting, Southern , Cloning, Molecular , Phenotype , Plasmids , Proline/metabolism , Repetitive Sequences, Nucleic Acid , Restriction MappingABSTRACT
DNA-mediated genetic transformation of Aspergillus nidulans has been achieved by incubating protoplasts from a strain of A. nidulans carrying a deletion in the acetamidase structural gene with DNA of derivatives of plasmid pBR322 containing the cloned structural gene for acetamidase [Hynes et al., Mol. Cell. Biol. 3 (1983) 1430-1439; p3SR2] in the presence of polyethylene glycol and CaCl2. The highest frequency obtained was 25 transformants per microgram of DNA. No enhancement of the transformation frequency was observed when DNAs of plasmids carrying either a fragment of the A. nidulans ribosomal repeat (p3SR2rr) or a fragment containing a possible A. nidulans mitochondrial origin of replication (p3SR2mo) in addition to the acetamidase gene were used. Both pBR322 and acetamidase gene sequences become integrated into the genome of A. nidulans in transformant strains. Integration events into the residual sequences adjacent to the deletion in the acetamidase gene, and probably (for p3SR2rr and p3SR2mo) into the ribosomal repeat unit are described.