Severe multi-year drought coincident with Hittite collapse around 1198-1196 BC.

The potential of climate change to substantially alter human history is a pressing concern, but the specific effects of different types of climate change remain unknown. This question can be addressed using palaeoclimatic and archaeological data. For instance, a 300-year, low-frequency shift to drier, cooler climate conditions around 1200 BC is frequently associated with the collapse of several ancient civilizations in the Eastern Mediterranean and Near East1-4. However, the precise details of synchronized climate and human-history-scale associations are lacking. The archaeological-historical record contains multiple instances of human societies successfully adapting to low-frequency climate change5-7. It is likely that consecutive multi-year occurrences of rare, unexpected extreme climatic events may push a population beyond adaptation and centuries-old resilience practices5,7-10. Here we examine the collapse of the Hittite Empire around 1200 BC. The Hittites were one of the great powers in the ancient world across five centuries11-14, with an empire centred in a semi-arid region in Anatolia with political and socioeconomic interconnections throughout the ancient Near East and Eastern Mediterranean, which for a long time proved resilient despite facing regular and intersecting sociopolitical, economic and environmental challenges. Examination of ring width and stable isotope records obtained from contemporary juniper trees in central Anatolia provides a high-resolution dryness record. This analysis identifies an unusually severe continuous dry period from around 1198 to 1196 (±3) BC, potentially indicating a tipping point, and signals the type of episode that can overwhelm contemporary risk-buffering practices.

Can heavy metal pollution induce bacterial resistance to heavy metals and antibiotics in soils from an ancient land-mine?

Microbial resistance to antibiotics is a growing challenge to human health. Recent evidence has indicated that antibiotic resistance can be co-selected for by exposure to heavy metals in agricultural soils. It remains unknown if this is a concern in other environments contaminated by metals. We here investigated soil microbial activities, composition and tolerance to heavy metals and antibiotics in a mining soil survey. We found that microbial respiration, growth, and biomass were affected by available metal concentrations. Most of the variation in microbial PLFA composition was explained by differences in heavy metal and pH. Additionally, pollution-induced bacterial community tolerance to toxicants including Cu, Pb, Zn, tetracycline and vancomycin was determined. Although only bacterial tolerance to Pb increased with higher levels of metals, the links between bacterial metal tolerance and soil metal concentrations were clear when considered together with previously published reports, suggesting that bacterial metal tolerance were universally elevated in the surveyed soils. The induced levels of heavy metal tolerance coincided with elevated levels of tolerance to vancomycin, but not to tetracycline. Our study showed that heavy metals can co-select for resistance to clinically important antibiotics also in ecosystems without manure input or antibiotic pollution.

Paleopathological study of a podal osteochondroma from the prehistoric Hypogeum of Calaforno (Sicily).

In this article, we report a case of isolated podal osteochondroma from the prehistoric Hypogeum of Calaforno (Giarratana, Ragusa, Sicily). Although the phalanx exhibiting the benign tumoral mass comes from a context featuring several commingled remains, the very good state of preservation of this bone allowed us to perform a comprehensive study of the neoplasm by applying a multidisciplinary approach encompassing archeology, morphology, stereomicroscopy, and radiology. The results from this very ancient specimen have been assessed in the light of the available paleopathological literature and clinical implications currently encountered in modern patients.

Genome-wide diversity and global migration patterns in dromedaries follow ancient caravan routes.

Dromedaries have been essential for the prosperity of civilizations in arid environments and the dispersal of humans, goods and cultures along ancient, cross-continental trading routes. With increasing desertification their importance as livestock species is rising rapidly, but little is known about their genome-wide diversity and demographic history. As previous studies using few nuclear markers found weak phylogeographic structure, here we detected fine-scale population differentiation in dromedaries across Asia and Africa by adopting a genome-wide approach. Global patterns of effective migration rates revealed pathways of dispersal after domestication, following historic caravan routes like the Silk and Incense Roads. Our results show that a Pleistocene bottleneck and Medieval expansions during the rise of the Ottoman empire have shaped genome-wide diversity in modern dromedaries. By understanding subtle population structure we recognize the value of small, locally adapted populations and appeal for securing genomic diversity for a sustainable utilization of this key desert species.

Fingerprint evidence for the division of labour and learning pottery-making at Early Bronze Age Tell es-Sâfi/Gath, Israel.

The organization of craft production has long been a marker for broader social, economic and political changes that accompanied urbanism. The identity of producers who comprised production groups, communities, or workshops is out of reach using conventional archaeological data. There has been some success using epidermal prints on artefacts to identify the age and sex of producers. However, forensic research indicates that a combination of ridge breadth and ridge density would best identify the age and sex of individuals. To this end, we combine mean ridge breadth (MRB) and mean ridge density (MRD) to distinguish the age and sex of 112 fingerprints on Early Bronze Age (EB) III pottery from the early urban neighbourhood at Tell es-Sâfi/Gath, Israel, dating to a 100 year time span. Our analysis accounts for the shrinkage of calcareous fabrics used to make six type of vessels, applies a modified version of the Kamp et al. regression equation to the MRB for each individual print, and infers sex by correlating MRD data to appropriate modern reference populations. When the results are combined, our analyses indicate that most fingerprints were made by adult and young males and the remainder by adult and young females. Children's prints are in evidence but only occur on handles. Multiple prints of different age and sex on the same vessels suggest they were impressed during the training of young potters. Production appears dominated by adult and young males working alone, together, and in cooperation with adult and/or young females. Vessels with prints exclusively by females of any age are rare. This male dominant cooperative labour pattern contrasts recent studies showing that adult women primarily made Neolithic figurines in Anatolia, and more females than males were making pottery prior to the rise of city-states in northern Mesopotamia.

Frankfurters made with pork meat, emmer wheat (Triticum dicoccum Schübler) and almonds nut (Prunus dulcis Mill.): evaluation during storage of a novel food from an ancient recipe.

The physical, chemical and microbiological characteristics of reformulated meat-based frankfurters, derived from an ancient Roman cookbook and produced from pork meat, emmer wheat, almond, fish sauce and spices, were investigated during storage. Two different formulations were considered, with pork thigh and pork shoulder muscles, respectively. The products contain 13-16% protein, 14-19% fat, 14% carbohydrates, >7% fibre and a polyunsaturated/saturated fatty acids ratio above 0.4. During the 24 days of storage, both products evidenced a decrease in the pH and increases in the total volatile nitrogen content and TBARs value, whereas, the aw remained stable. From a microbiological perspective, increases in the total viable count and lactic acid bacteria up to 4.8 log cfu/g occurred during storage, but no pathogens were found. Sensory analyses revealed a change in odour and flavour at 18 days, with the detection of a fermented and rancid taste. Survival sensory analysis defined a shelf life of the products of between 18.6 and 22.7 days.

Heterogeneity in global vegetation and terrestrial climate change during the late Eocene to early Oligocene transition.

Rapid global cooling at the Eocene - Oligocene Transition (EOT), ~33.9-33.5 Ma, is widely considered to mark the onset of the modern icehouse world. A large and rapid drop in atmospheric pCO2 has been proposed as the driving force behind extinctions in the marine realm and glaciation on Antarctica. However, the global terrestrial response to this cooling is uncertain. Here we present the first global vegetation and terrestrial temperature reconstructions for the EOT. Using an extensive palynological dataset, that has been statistically grouped into palaeo-biomes, we show a more transitional nature of terrestrial climate change by indicating a spatial and temporal heterogeneity of vegetation change at the EOT in both hemispheres. The reconstructed terrestrial temperatures show for many regions a cooling that started well before the EOT and continued into the Early Oligocene. We conclude that the heterogeneous pattern of global vegetation change has been controlled by a combination of multiple forcings, such as tectonics, sea-level fall and long-term decline in greenhouse gas concentrations during the late Eocene to early Oligocene, and does not represent a single response to a rapid decline in atmospheric pCO2 at the EOT.

Whence 'zoster'? The convoluted classical origins of a sometimes illogical term.

The term 'zoster' is nowadays associated with 'herpes zoster', the condition resulting from reactivation of the latent varicella-zoster virus which causes shingles. But in antiquity the meaning of 'zoster', a Latin word originating from the Greek for a belt or girdle, was variously associated in men with a form of body armour which could enclose just one half of the body; in women with a garment worn around the waist and sometimes called a 'zona'; and with a place, Zoster, linked mythologically then with the goddess Leto and her zona. Around 48 AD, the Roman physician Scribonius Largus became the first to associate 'zona' with 'herpes', and to attribute a medical meaning to 'zona', here an abbreviation of 'zona ignea' ('fiery girdle'). Although in the past the terms 'zoster' and 'zona' were sometimes used interchangeably, today only 'zoster' remains-even when etymologically illogical in those patients whose zoster rash occurs in body areas other than the trunk.

Mobile NMR: An essential tool for protecting our cultural heritage.

What is 'cultural heritage'? Is it simply our legacy of physical artifacts - or is it our collective legacy as human societies - how we want to be remembered by future generations? With time, negligence, and even military conflict working to erase the past, we must ask: Can a better understanding of our shared heritage assists us in addressing cultural differences in the present day? And how can science both help us understand the historic record and work to preserve it? In this perspective article, we examine an emerging scientific method, mobile nuclear magnetic resonance (NMR), which can help us examine in a non-invasive way important objects and sites of our cultural heritage. Following these investigations, one can envisage ways for protecting our global heritage for future generations. For this purpose, we examine how this method can be used to non-destructively explore historical artifacts, which can lead to understanding the science behind the creation of these treasured items - paintings, frescoes, parchments, historical buildings, musical instruments, ancient mummies, and other artifacts. This perspective article follows few relevant examples from the scientific literature where mobile NMR has been applied in a non-invasive way to analyze objects of cultural heritage. One can envision possible future advancements of this technique and further applications where portable NMR can be used for conservation of cultural heritage. Copyright © 2016 John Wiley & Sons, Ltd.

Historical anthropogenic contributions to mercury accumulation recorded by a peat core from Dajiuhu montane mire, central China.

Mercury (Hg) accumulation records spanning the last 16,000 years before present (yr BP, relative to AD 1950) were derived from a peat core collected from Dajiuhu mire, central China. The natural Hg concentration and accumulation rate (free from anthropogenic influence) were 135.5 ± 53.9 ng g(-1) and 6.5 ± 4.5 µg m(-2) yr(-1), respectively. The increase in Hg flux that started from a core depth of 96.5 cm (3358 cal yr BP) is independent of soil erosion and organic matter content. We attribute this to an increase in atmospheric Hg deposition derived from regional anthropogenic activities. Anthropogenic Hg accumulation rates (Hg-ARA) in the pre-industrial period peaked during the Ming and the early Qing dynasties (582-100 cal yr BP), with Hg-ARA of 9.9-24.6 and 10.7-24.4 µg m(-2) yr(-1), respectively. In the industrial interval (post∼1850 AD), Hg-ARA increased progressively and reached 32.7 µg m(-2) yr(-1) at the top of the core. Our results indicate the existence of regional atmospheric Hg pollution spanning the past ∼3400 years, and place recent Hg enrichment in central China in a broader historical context.

Hammurabi's Code: A primary datum in the conjoined professions of medicine and law.

The history of our current law dates from Palaeolithic times. The first written laws were codified by the rulers of Mesopotamian kingdoms, from the beginning of the second millennium B.C.E. This history, and those of the medico-legal specialties in particular, trace their origins to Hammurabi's Code. Hammurabi (ruled 1792-1750 B.C.E.) was the sixth King of the First Dynasty of ancient Babylon, today an archaeological site in modern-day Iraq. Hammurabi's Laws (c.1760 B.C.E.), inscribed on at least one diorite stele, were set up in public places in Babylon, towards the end of the King's 43-year reign. Comprising almost 300 specific laws, with judicial punishment for transgressions, Hammurabi's Code reflects his role both as a guardian of the vulnerable and as a protector of the weak and powerless. Just as medical papyri from ancient Egypt (e.g. the Edwin Smith papyrus c.1600 B.C.E.) are regarded as the origins of western medicine, so Hammurabi is the pioneer of "medical" laws as these have evolved to their sophisticated state today.

[The legal status of blood's therapeutic products and consequences]. / Le statut juridique des produits thérapeutiques issus du sang et ses conséquences.

The first testimonies of humanity show a constant: blood was always surrounded with a sacred fascination. For years, it was not useful to define a legal status for blood, but healthy crisis, medical practices, had led to answer this question in a context of legal protection of human dignity and rights. Actually, the legal frame is moving and to know those statuses is helpful for a better understanding of the changes.

[The prehistory of orthodontics]. / La préhistoire de l'orthodontie.

Orthodontics came into being in 1728. Previously, practitioners were at a loss when confronted with crooked teeth. A Latin philosopher had an ingenious flash of orthodontic inspiration. Other authors were content to either extract the malposed teeth or to modify their shape. However, interest in an approach to preventive orthodontics had now begun.

[THE HISTORY OF SKIN GRAFTING, ANCIENT TIMES AND CIVILIZATIONS. THE FIRST MENTIONS].

The article dials with the history of medicine practiced by the first civilizations. The facts of discovery and analysis of ancient medical treatises by modern researchers are reported. Special emphasis is laid on the achievements of medical practitioners in Ancient Egypt and India that promoted the development of operative treatment including skin grafting.

History of venereal diseases from antiquity to the renaissance.

Sexually transmitted diseases (STDs), previously known as venereal diseases (VD), were present among the populations of antiquity as well as during the Middle Ages. Clay tablets from Mesopotamia, Egyptian papyri, along with mythology, paintings of erotic scenes, and presence of prostitutes give sufficient information to assume that some form of urethral and vaginal discharge, and also herpes genitalis were present among people at that time, and that these diseases were considered a divine punishment. Some passages of the Bible say much about the sexual behavior of the ancient Hebrews. The writings of the Greek and Roman physicians and of their satiric poets (Martial, Juvenal, Ovid) described diverse genital diseases. Celsus described various diseases of the genitals, that he called the "obscene parts". Galen made a strange description of the female genitals and coined the term gonorrhea - flow of semen. The ancient Chinese and Indian physicians also gave some account on the presence of venereal diseases in their books, and the temple sculptures depict their sexual life. During the Middle Ages, numerous physicians and surgeons from Europe as well as from Arabic countries wrote on local diseases of the genitals, describing chancres, condylomata, erosions, pustules, urethral and vaginal discharge, and their treatment. Some were aware that the alterations were connected with sexual activity. In spite the fact the Christian church propagated abstinence, the spread of venereal diseases was possible because the diffusion of prostitution, communal baths, and wars. During the 19th century, some of the physicians and historians, especially J. Rosenbaum, F. Buret, and E. Lancereaux believed syphilis was as old as mankind, whereas later authors had the opinion the disease appeared at the end of the 15th century.

[Mycobacterium infection in prehistoric humans: co-evolution in remote ages]. / L'infezione da micobatteri nell'uomo preistorico. Un cammino parallelo (co-evoluzione) dimostrato già in epoche remote.

The introduction of agriculture and animal husbandry at the end of the Mesolithic era, despite enabling a significant demographic growth through an increase in food storage and availability, caused new infectious noxae to enter the pathocoenosis. However in the Palaeolithic era, hunter-gatherers were already in contact with infectious diseases of animal origin, albeit episodically. Modern biomedical technologies allow us to estimate, with better approximation, how long mankind has been in contact with Mycobacterium tuberculosis. Archaeological finds, including human and animal remains (especially the aurochs), are particularly studied by palaeopathologists, as mycobacteria frequently cause bone involvement and this characteristic is of particular interest for palaeopathological (even macroscopic) studies; the interest is to detect the ancient DNA of MT, which is the cause of bone tuberculosis in skeletal remains as well as in mummies. According to our present knowledge, palaeopathological findings, confirmed by molecular techniques, suggest that tuberculosis in human skeletons goes back at most to 9000 years ago, while, in a veterinary environment, the most ancient DNA of MTBC to be detected in an American bison dates back about 17,000 years. The possibility of discovering archaeological finds making even more ancient human remains available leaves opens up the possibility of dating back to previous eras the transmission of MTBC infection to mankind. Phylogenetic works examining the available materials (DNAa) suggest that Mycobacterium tuberculosis, the cause of tuberculosis infection in humans and cattle (Aurochs), would have had a co-evolutionary process. On the basis of recent phylogenetic studies, the MTBC genome would have had a wide span of time to reach a suitable adjustment, co-evolving in geographical environments both at high and low host density. It is likely that the strains that did not show this strong "flexibility" underwent extinction, in favour of more versatile, adaptable strains, that are able to infect susceptible hosts "always" and in any environmental condition.

[THE NASCENCY OF DRUG AND FIRST STEPS OF ITS DEVELOPMENT].

In this historical review article, the first examples of human attempts to use drugs for the solution of their health problems and the development of the concept of natural toxic (poisonous) substances in the history and prehistoric times, are discussed. It is regarded that, outside of the quelling of the hunger or quenching the thirst, some of our early an- cestors have coincidentally began to distinguish the different effects in some of the natural products in their environment, probably during their nutritional behavior. It is thought that in this way, the awareness for drugs and toxic substances have been raised. In our available sources related to this topic, the first instance in different societies that can be considered as drug seems to belong to different dates, and strikingly the earliest drug samples were found particularly in ancient Mesopotamia, Egypt, China and Indian civilizations. It was determined that primarily herbal resources were utilized for the first instance that can be considered as the first drug application in discussed societies. On the other hand, these first natural origin drug samples have been remained in the use of humankind in later centuries and some of them seems to be applied in a certain manner even today.

["Human races": history of a dangerous illusion]. / Les "races humaines": histoire d'une illusion dangereuse.

The multiplication of offences prompted by racism and the increase of complaints for racism leads us to consider the illusory concept of "human races". This idea crossed the history, and was reinforced by the discovery of remote tribes and human fossils, and by the development of sociobiology and quantitative psychology. Deprived of scientific base, the theory of the "races" must bow before the notions of genetic variation and unicity of mankind.

Anatomical and genetic study of an ancient animal tooth showing brachyodont and hypsodont mixed taxonomical characteristics.

A non-human dental piece was found in a Roman Empire tomb dated the 3rd century A.C. in Zaragoza (Spain). The morphology of this piece showed mixed brachyodont (carnivores) and hypsodont (herbivores) characteristics. As a result, the taxonomical assignation of the piece was impossible. Therefore, a protocol based on the DNA sequence of the cytochrome c oxidase subunit 1 mitochondrial region (COI) was applied. For this purpose, a pair of primers able to amplify this region in a large variety of animals was designed. The results point to a species of the Genus Bos (Family Bovidae). This assignation was later confirmed by these quencing of a short fragment of the mitochondrial D-loop region. A complete morphological description of the tooth is presented together with the DNA sequence study and comparison protocol.