ABSTRACT
A unified synthetic route for the total syntheses of eribulin and a macrolactam analog of halichondrin B is described. The key to the success of the current synthetic approach includes the employment of our reverse approach for the construction of cyclic ether structural motifs and a modified intramolecular cyclization reaction between alkyl iodide and aldehyde functionalities to establish the all-carbon macrocyclic framework of eribulin. These syntheses, together with our previous work on the total syntheses of halichondrin B and norhalichondrin B, demonstrate and validate the powerful reverse approach in the construction of cyclic ether structural motifs. On the other hand, the unified synthetic strategy for the synthesis of the related macrolactam analog provides inspiration and opportunities in the halichondrin field and related polycyclic ether areas.
Subject(s)
Ethers, Cyclic , Furans , Ketones , Macrolides , Ethers, Cyclic/chemical synthesis , Furans/chemical synthesis , Ketones/chemical synthesis , Macrolides/chemical synthesisABSTRACT
An original synthetic route was developed for the preparation of previously unknown unsaturated polyaromatic macrolactones containing a 1Z,5Z-diene moiety in 48-71% yields and with >98% stereoselectivity. The method is based on intermolecular cyclocondensation of aromatic dicarboxylic acids with α,ω-alka-nZ,(n+4)Z-dienediols (1,12-dodeca-4Z,8Z-dienediol, 1,14-tetradeca-5Z,9Z-dienediol, 1,18-octadeca-7Z,11Z-dienediol) mediated by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC)/4-dimethylaminopyridine (DMAP). The unsaturated diols were prepared by successive homo-cyclomagnesiation of tetrahydropyran ethers of O-containing 1,2-dienes with EtMgBr in the presence of Mg metal and the Cp2TiCl2 catalyst (10 mol.%) and subsequent treatment with 0.1 equiv. of para-toluenesulfonic acid of pyran ethers formed after the acid hydrolysis of magnesacyclopentanes. The resulting cyclophanes exhibited high cytotoxic activity in vitro against Jurkat, K562, U937, and HL60 cancer lines. Additionally, the synthesized products were studied for their effect on mitochondria, ability to induce apoptosis, and influence on the cell cycle using modern flow cytometry methods.
Subject(s)
Antineoplastic Agents/chemical synthesis , Ethers, Cyclic/chemical synthesis , Organometallic Compounds/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Cell Line, Tumor , Cell Proliferation/drug effects , Cell Survival/drug effects , Cycloaddition Reaction , Ethers, Cyclic/chemistry , Ethers, Cyclic/pharmacology , Flow Cytometry , HL-60 Cells , Humans , Jurkat Cells , K562 Cells , Molecular StructureABSTRACT
Marine polycyclic ether natural products have gained significant interest from the chemical community due to their impressively huge molecular architecture and diverse biological functions. The structure assignment of this class of extraordinarily complex natural products has mainly relied on NMR spectroscopic analysis. However, NMR spectroscopic analysis has its own limitations, including configurational assignment of stereogenic centers within conformationally flexible systems. Chemical shift deviation analysis of synthetic model compounds is a reliable means to assign the relative configuration of "difficult" stereogenic centers. The complete configurational assignment must be ultimately established through total synthesis. The aim of this review is to summarize the indispensable role of organic synthesis in stereochemical assignment of marine polycyclic ethers.
Subject(s)
Aquatic Organisms/metabolism , Ethers, Cyclic/chemical synthesis , Chemistry Techniques, Synthetic , Ciguatoxins/chemical synthesis , Ciguatoxins/isolation & purification , Ethers/chemical synthesis , Ethers/isolation & purification , Ethers, Cyclic/isolation & purification , Humans , Magnetic Resonance Spectroscopy , Marine Toxins/chemical synthesis , Marine Toxins/isolation & purification , Molecular Structure , Oxocins/chemical synthesis , Oxocins/isolation & purification , Polymers/chemical synthesis , Polymers/isolation & purification , Secondary Metabolism , Stereoisomerism , Structure-Activity RelationshipABSTRACT
We report a practical two-step approach involving a Ugi 4-CR/ azide-alkyne cycloaddition for the synthesis of biaryl-containing cyclophanes. The series represents an extension of our previously reported macrocycles as an effort to enhance the anti-proliferative activity of this scaffold. In this variant, we incorporate a biphenyl moiety in the framework, thus enhancing the macrocycle size, lipophilicity, and structural diversity. Macrocycles were tested against different cell lines, being more cytotoxic against prostate (PC-3 and DU-145) and breast (MCF-7) tumor cells. Gratifyingly, the most active compound showed a significative enhancement of PC-3 growth inhibition with respect to our previous series, reaffirming the potential anti-proliferative activity of this kind of cyclophanes.
Subject(s)
Antineoplastic Agents/chemical synthesis , Ethers, Cyclic/chemical synthesis , Piperidines/chemical synthesis , Triazoles/chemistry , Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Biphenyl Compounds/chemistry , Cell Line, Tumor , Cell Proliferation/drug effects , Cycloaddition Reaction , Drug Screening Assays, Antitumor , Ethers, Cyclic/pharmacology , Humans , Piperidines/pharmacology , Structure-Activity RelationshipABSTRACT
The high-yield synthesis and the structural investigation of a new cryptand with C3 symmetry, exhibiting 2,4,6-triphenyl-1,3,5-triazine central units and pyridine-based bridges, are reported. The structure of the compound was investigated by single crystal X-ray diffractometry, NMR (nuclear magnetic resonance), HRMS (high resolution mass spectrometry) measurements, and theoretical calculations. The study of supramolecular behavior in solid state revealed the association of cryptand molecules by C-H---π and π---π contacts. Moreover, theoretical calculations indicated the high binding affinity of the cryptand for various organic molecules as guests.
Subject(s)
Ethers, Cyclic/chemistry , Ethers, Cyclic/chemical synthesis , Models, Molecular , Pyridines/chemistry , Schiff Bases/chemistry , Schiff Bases/chemical synthesis , Crystallography, X-Ray , Magnetic Resonance Spectroscopy , Molecular StructureABSTRACT
One of the most efficient ways to synthesize oxetanes is the light-enabled [2 + 2] cycloaddition reaction of carbonyls and alkenes, referred to as the Paternò-Büchi reaction. The reaction conditions for this transformation typically require the use of high energy UV light to excite the carbonyl, limiting the applications, safety, and scalability. We herein report the development of a visible-light-mediated Paternò-Büchi reaction protocol that relies on triplet energy transfer from an iridium-based photocatalyst to the carbonyl substrates. This mode of activation is demonstrated for a variety of aryl glyoxylates and negates the need for both visible-light-absorbing carbonyl starting materials and UV light to enable access to a variety of functionalized oxetanes in up to 99% yield.
Subject(s)
Alkenes/chemistry , Benzophenones/chemistry , Ethers, Cyclic/chemical synthesis , Cycloaddition Reaction , Energy Transfer , Ethers, Cyclic/chemistry , Light , Molecular Structure , Photochemistry , Stereoisomerism , Ultraviolet RaysABSTRACT
The synthesis and use of oxetane modified dipeptide building blocks in solution and solid-phase peptide synthesis (SPPS) is reported. The preparation of building blocks containing non-glycine residues at the N-terminus in a stereochemically controlled manner is challenging. Here, a practical 4-step route to such building blocks is demonstrated, through the synthesis of dipeptides containing contiguous alanine residues. The incorporation of these new derivatives at specific sites along the backbone of an alanine-rich peptide sequence containing eighteen amino acids is demonstrated via solid-phase peptide synthesis. Additionally, new methods to enable the incorporation of all 20 of the proteinogenic amino acids into such dipeptide building blocks are reported through modifications of the synthetic route (for Cys and Met) and by changes to the protecting group strategy (for His, Ser and Thr).
Subject(s)
Dipeptides/antagonists & inhibitors , Dipeptides/chemical synthesis , Drug Development , Ethers, Cyclic/pharmacology , Solid-Phase Synthesis Techniques , Dipeptides/chemistry , Ethers, Cyclic/chemical synthesis , Ethers, Cyclic/chemistry , Molecular StructureABSTRACT
Ruthenium alkoxymethylidene complexes have rarely been demonstrated as active species in metathesis reactions and are frequently regarded as inert. Herein, we highlight the ability of these Fischer-type carbenes to participate in cascade alternating ring-opening metathesis polymerization through their efficient alkyne addition reactions. When enyne monomers are combined with low-strain cyclic vinyl ethers, a controlled chain-growth copolymerization occurs that exhibits high degrees of alternation (>90% alternating diads) and produces degradable poly(vinyl ether) materials with low dispersities and targetable molecular weights. This new method is amenable to the synthesis of alternating diblock polymers that can be degraded to small-molecule fragments under aqueous acidic conditions. This work furthers the potential of Fischer-type ruthenium alkylidenes in polymerization strategies and presents new avenues for the generation of functional metathesis materials.
Subject(s)
Alkynes/chemical synthesis , Ethers, Cyclic/chemical synthesis , Methane/analogs & derivatives , Organometallic Compounds/chemistry , Ruthenium/chemistry , Alkynes/chemistry , Ethers, Cyclic/chemistry , Methane/chemistry , Molecular Structure , PolymerizationABSTRACT
The copper-catalyzed enantioselective intramolecular hydroalkoxylation of unactivated alkenes for the synthesis of tetrahydrofurans, phthalans, isochromans, and morpholines from 4- and 5-alkenols is reported. The substrate scope is complementary to existing enantioselective alkene hydroalkoxylations and is broad with respect to substrate backbone and alkene substitution. The asymmetric induction and isotopic labeling studies support a polar/radical mechanism involving enantioselective oxycupration followed by C-[Cu] homolysis and hydrogen atom transfer. Synthesis of the antifungal insecticide furametpyr was accomplished.
Subject(s)
Alkenes/chemistry , Antifungal Agents/chemical synthesis , Benzofurans/chemical synthesis , Copper/chemistry , Ethers, Cyclic/chemistry , Ethers, Cyclic/chemical synthesis , Insecticides/chemical synthesis , Pyrazoles/chemical synthesis , Antifungal Agents/chemistry , Benzofurans/chemistry , Catalysis , Furans/chemistry , Hydrogen/chemistry , Insecticides/chemistry , Molecular Structure , Pyrazoles/chemistry , StereoisomerismABSTRACT
The [2 + 2] photocycloaddition of alkenes and carbonyls is of fundamental interest and practical importance, as this process is extensively involved in oxetane-ring constructions. Although individual carbonyl group or alkene moiety has been utilized as photoactive species for oxetane formations upon ultraviolet photoexcitation, direct excitation of the entire noncovalent complex involving alkene and carbonyl substrates to achieve [2 + 2] photocycloadditions is rarely addressed. Herein, complexes with noncovalent interactions between benzophenone and CâC bonds in unsaturated lipids have been successfully characterized, and for the first time a [2 + 2] cycloaddition leading to the formation of oxetanes has been identified under visible-light irradiation. The mechanism of this reaction is distinctly different from the well-studied Paternò-Büchi reaction. The entire complexes characterized as dimeric proton-bonded alkene and carbonyl substrates can be excited under visible light, leading to electron transfer from the alkene moiety in fatty acyls to the carbonyl group within the complex. These results provide new insight into utilizing noncovalent complexes for the synthesis of oxetanes in which the excitation wavelength becomes independent of each individual substrate.
Subject(s)
Benzophenones/chemistry , Fatty Acids, Unsaturated/chemistry , Cycloaddition Reaction , Ethers, Cyclic/chemical synthesis , Models, Molecular , Photochemical Processes , Quantum Theory , Spectrometry, Mass, Electrospray IonizationABSTRACT
Saturated oxygen heterocycles are widely found in a broad array of natural products and other biologically active molecules. In medicinal chemistry, small and medium rings are also important synthetic intermediates since they can undergo ring-opening and -expansion reactions. These applications have driven numerous studies on the synthesis of oxygen-containing heterocycles and considerable effort has been devoted toward the development of methods for the construction of saturated oxygen heterocycles. This paper provides an overview of the biological roles and synthetic strategies of saturated cyclic ethers, covering some of the most studied and newly discovered related natural products in recent years. This paper also reports several promising and newly developed synthetic methods, emphasizing 3-7 membered rings.
Subject(s)
Biological Products/chemistry , Ethers, Cyclic/chemical synthesis , Animals , Biological Products/pharmacology , Chemistry, Pharmaceutical , Drug Evaluation, Preclinical , Ethers, Cyclic/chemistry , Ethers, Cyclic/pharmacology , Humans , Molecular Structure , Oxygen/chemistryABSTRACT
Macrocyclic hosts have long been the workhorses of molecular recognition. Despite the widespread use of container-shaped molecules as synthetic receptors, an efficient preparation of cavitands bearing multiple functional groups has not been realized. This Letter describes a new cavitand derived from a sequence-defined oligoamide foldamer scaffold. A solid-phase synthesis approach is reported, which enables the display of multiple chemically diverse functional groups on the cavitand rim.
Subject(s)
Ethers, Cyclic/chemical synthesis , Macrocyclic Compounds/chemical synthesis , Resorcinols/chemical synthesis , Cyclization , Ethers, Cyclic/chemistry , Macrocyclic Compounds/chemistry , Molecular Structure , Resorcinols/chemistry , Solid-Phase Synthesis TechniquesABSTRACT
We describe the design, synthesis, and biological evaluation of novel HIV-1 protease inhibitors containing a squaramide-derived scaffold as the P2 ligand in combination with a (R)-hydroxyethylamine sulfonamide isostere. Inhibitor 3h with an N-methyl-3-(R)-aminotetrahydrofuranyl squaramide P2-ligand displayed an HIV-1 protease inhibitory Ki value of 0.51â¯nM. An energy minimized model of 3h revealed the major molecular interactions between HIV-1 protease active site and the tetrahydrofuranyl squaramide scaffold that may be responsible for its potent activity.
Subject(s)
Drug Design , Ethers, Cyclic/pharmacology , HIV Protease Inhibitors/pharmacology , HIV Protease/metabolism , Quinine/analogs & derivatives , Dose-Response Relationship, Drug , Ethers, Cyclic/chemical synthesis , Ethers, Cyclic/chemistry , HIV Protease Inhibitors/chemical synthesis , HIV Protease Inhibitors/chemistry , Humans , Ligands , Molecular Structure , Quinine/chemical synthesis , Quinine/chemistry , Quinine/pharmacology , Structure-Activity RelationshipABSTRACT
Herein, we report the first total synthesis of marine ladder polyether gymnocin B (1) based on a two-phase strategy. In Phase I, inspired by the proposed biosynthesis, epoxide-opening cascades assemble 10 out of 15 cyclic ether rings making up the molecular core. In the subsequent Phase II, coalescence elevates the molecular complexity further by coupling of these subunits. Our two-phase synthetic approach significantly improved the step efficiency of the synthesis of this class of natural products.
Subject(s)
Biological Products/chemical synthesis , Ethers, Cyclic/chemical synthesis , Biological Products/chemistry , Ethers, Cyclic/chemistry , Molecular ConformationABSTRACT
Hydroalkoxylation is a useful and efficient reaction which generates C-O bond and produces cyclic ethers, the common structural elements of natural products. The dedicative enzyme which can catalyze enantioselective hydroalkoxylation named PhnH was recently identified in the herqueinone biosynthetic gene from Penicillium herquei. It catalyzes addition of a phenol to the terminal olefin on substrate to produce a dihydrobenzofuran. Here, the crystal structure of PhnH is reported and the putative substrate-binding pocket is illustrated. Through docking experiment, possible substrate-binding poses are displayed and the catalytic mechanism is therefore proposed. Our findings form the basis for further studies of enantioselective hydroalkoxylation enzymes.
Subject(s)
Fungal Proteins/chemistry , Penicillium/enzymology , Phenalenes/chemical synthesis , Alcohols/chemistry , Benzofurans/chemistry , Binding Sites , Catalysis , Cloning, Molecular , Crystallography, X-Ray , Escherichia coli/genetics , Escherichia coli/metabolism , Ethers, Cyclic/chemical synthesis , Fungal Proteins/genetics , Fungal Proteins/metabolism , Gene Expression , Genetic Vectors/chemistry , Genetic Vectors/metabolism , Molecular Docking Simulation , Penicillium/chemistry , Phenalenes/metabolism , Phenols/chemistry , Protein Binding , Protein Conformation, alpha-Helical , Protein Conformation, beta-Strand , Protein Interaction Domains and Motifs , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Stereoisomerism , Substrate SpecificityABSTRACT
Despite their outstanding antitumour activity in mice, the limited supply from the natural sources has prevented drug discovery/development based on intact halichondrins. We achieved a total synthesis of C52-halichondrin-B amine (E7130) on a >10 g scale with >99.8% purity under GMP conditions. Interestingly, E7130 not only is a novel microtubule dynamics inhibitor but can also increase intratumoural CD31-positive endothelial cells and reduce α-SMA-positive cancer-associated fibroblasts at pharmacologically relevant compound concentrations. According to these unique effects, E7130 significantly augment the effect of antitumour treatments in mouse models and is currently in a clinical trial. Overall, our work demonstrates that a total synthesis can address the issue of limited material supply in drug discovery/development even for the cases of complex natural products.
Subject(s)
Antineoplastic Agents, Phytogenic/chemical synthesis , Breast Neoplasms/drug therapy , Carcinoma, Squamous Cell/drug therapy , Ethers, Cyclic/chemical synthesis , Head and Neck Neoplasms/drug therapy , Macrolides/chemical synthesis , Tubulin Modulators/chemical synthesis , Actins/genetics , Actins/metabolism , Animals , Antineoplastic Agents, Phytogenic/pharmacology , Antineoplastic Combined Chemotherapy Protocols , Biological Products/chemical synthesis , Biological Products/pharmacology , Breast Neoplasms/mortality , Breast Neoplasms/pathology , Cancer-Associated Fibroblasts/drug effects , Cancer-Associated Fibroblasts/metabolism , Cancer-Associated Fibroblasts/pathology , Carcinoma, Squamous Cell/mortality , Carcinoma, Squamous Cell/pathology , Cetuximab/pharmacology , Drug Discovery , Endothelial Cells/drug effects , Endothelial Cells/metabolism , Endothelial Cells/pathology , Ethers, Cyclic/pharmacology , Female , Gene Expression/drug effects , Head and Neck Neoplasms/mortality , Head and Neck Neoplasms/pathology , Humans , Macrolides/pharmacology , Mice , Mice, Inbred BALB C , Platelet Endothelial Cell Adhesion Molecule-1/genetics , Platelet Endothelial Cell Adhesion Molecule-1/metabolism , Survival Analysis , Tubulin Modulators/pharmacology , Tumor Burden/drug effects , Xenograft Model Antitumor AssaysABSTRACT
This work characterizes a previously undetected epimerization in the preparation of alkynyl diols from pentose precursors utilizing the Ohira-Bestmann reagent. Lithium trimethylsilyldiazomethane (Colvin reagent) additions to the d-ribose and d-lyxose-derived benzylidene acetals provide the respective alkynyl diol stereoisomers, without epimerization. Regioselective tungsten-catalyzed cycloisomerizations of the d-ribose- and d-lyxose-derived alkynyl diols yield rigid bicyclic pyranose glycals, confirming the stereochemical fidelity of the Colvin alkynylation process.
Subject(s)
Alkynes/chemistry , Ethers, Cyclic/chemical synthesis , Pentoses/chemistry , Acetals/chemistry , Alcohols/chemistry , Catalysis , Lithium Compounds/chemistry , Molecular Structure , Ribose/chemistry , Stereoisomerism , Tungsten/chemistryABSTRACT
We previously showed that a naturally occurring macrocyclic bis(bibenzyl) derivative, riccardin C (RC), exhibits antibacterial activity towards methicillin-resistant Staphylococcus aureus (MRSA), with a potency comparable to that of the clinically used drug vancomycin. Here, we synthesized a series of RC derivatives to explore the structure-activity relationships (SAR). The SAR results clearly indicated that the number and positions of the phenolic hydroxyl groups are primary determinants of the anti-MRSA activity. Pharmacological characterization of the macrocyclic bis(bibenzyl) derivatives, together with fragment compounds and their dimers, indicated that the macrocycles and the fragment compounds elicit anti-MRSA activity with different mechanism(s) of action. The macrocyclic bis(bibenzyl)s are bactericidal, while the fragment compounds are bacteriostatic, showing only weak bactericidal activity. Treatment with a macrocyclic bis(bibenzyl) derivative significantly changed the intracellular Na+ and K+ concentrations of Staphylococcus aureus, and transmission electron microscopy revealed that treated cells developed intracellular lamellar mesosomal-like structures. These results indicated that the macrocyclic compound directly damages the gram-positive bacterial membrane, resulting in increased permeability.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/pharmacology , Drug Design , Ethers, Cyclic/chemical synthesis , Ethers, Cyclic/pharmacology , Methicillin-Resistant Staphylococcus aureus/drug effects , Cell Membrane Permeability/drug effects , Drug Resistance, Bacterial , Ethers, Cyclic/chemistry , Methicillin-Resistant Staphylococcus aureus/cytology , Methicillin-Resistant Staphylococcus aureus/metabolism , Methicillin-Resistant Staphylococcus aureus/ultrastructure , Microscopy, Electron, Transmission , Potassium/metabolism , Sodium/metabolism , Structure-Activity RelationshipABSTRACT
Marine natural products and biologically active compounds often contain cyclic ether units. Thus, regio- and stereoselective construction of these structures has long been a topic of interest in organic synthesis. This review summarizes new synthetic approaches to polycyclic ether natural products utilizing the features of chemical elements.
Subject(s)
Biological Products/chemical synthesis , Elements , Ethers, Cyclic/chemical synthesis , Marine Biology , Marine Toxins/chemical synthesis , Oxocins/chemical synthesis , Animals , Biological Products/chemistry , Catalysis , Chemistry, Organic/methods , Ethers, Cyclic/chemistry , Gold , Hydrophobic and Hydrophilic Interactions , Marine Toxins/chemistry , Mice , Molecular Conformation , Organic Chemistry Phenomena , Oxocins/chemistry , Rhenium/chemistry , StereoisomerismABSTRACT
Maleimide-thiol coupling is a popular bioconjugation strategy, but little is known about the stereoselectivity and the stereodynamics of the succinimide thioether formed in a biopolymer environment. We used thiol 1,4-addition for the macrocyclisation of 5 designed pentapeptides with the ringsize of hexapeptides because they incorporate the succinimide thioether (4-8). Both succinimide diastereomers are observed in the constrained macrocyclic rings in each case. In spite of the low diastereoselectivity of the macrocyclisation reaction, there is a significant influence of the amino acid environment on the epimerization rate of the succinimide. Its half life can be as short as several hours at room temperature when Gly is the amino acid following the succinimide (peptide 8). On the contrary, no epimerization is detectable even after several weeks in the case of d-Phe C-terminal to the succinimide in peptide 4. Already the small selection of examples shows how big the differences in epimerization rates can be and that the local environment has a significant influence. The variation of amino acids in the vicinity of the ligation site points the way towards the synthesis of bioconjugates which are obtained as stable and separable diastereomers.