Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters











Language
Publication year range
1.
Braz. j. med. biol. res ; 34(6): 699-709, Jun. 2001. ilus, tab, graf
Article in English | LILACS | ID: lil-285842

ABSTRACT

The anticlotting and antithrombotic activities of heparin, heparan sulfate, low molecular weight heparins, heparin and heparin-like compounds from various sources used in clinical practice or under development are briefly reviewed. Heparin isolated from shrimp mimics the pharmacological activities of low molecular weight heparins. A heparan sulfate from Artemia franciscana and a dermatan sulfate from tuna fish show a potent heparin cofactor II activity. A heparan sulfate derived from bovine pancreas has a potent antithrombotic activity in an arterial and venous thrombosis model with a negligible activity upon the serine proteases of the coagulation cascade. It is suggested that the antithrombotic activity of heparin and other antithrombotic agents is due at least in part to their action on endothelial cells stimulating the synthesis of an antithrombotic heparan sulfate.


Subject(s)
Humans , Animals , Cattle , Anticoagulants/pharmacology , Endothelium, Vascular/cytology , Fibrinolytic Agents/pharmacology , Heparin/pharmacology , Heparitin Sulfate/pharmacology , Anticoagulants/chemistry , Anticoagulants/metabolism , Crustacea , Fibrinolytic Agents/chemistry , Fibrinolytic Agents/metabolism , Glycosaminoglycans/metabolism , Glycosaminoglycans/pharmacology , Heparin, Low-Molecular-Weight/chemistry , Heparin, Low-Molecular-Weight/metabolism , Heparin, Low-Molecular-Weight/pharmacology , Heparin/metabolism , Heparitin Sulfate/biosynthesis , Tuna
2.
Braz J Med Biol Res ; 34(6): 699-709, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11378657

ABSTRACT

The anticlotting and antithrombotic activities of heparin, heparan sulfate, low molecular weight heparins, heparin and heparin-like compounds from various sources used in clinical practice or under development are briefly reviewed. Heparin isolated from shrimp mimics the pharmacological activities of low molecular weight heparins. A heparan sulfate from Artemia franciscana and a dermatan sulfate from tuna fish show a potent heparin cofactor II activity. A heparan sulfate derived from bovine pancreas has a potent antithrombotic activity in an arterial and venous thrombosis model with a negligible activity upon the serine proteases of the coagulation cascade. It is suggested that the antithrombotic activity of heparin and other antithrombotic agents is due at least in part to their action on endothelial cells stimulating the synthesis of an antithrombotic heparan sulfate.


Subject(s)
Anticoagulants/pharmacology , Endothelium, Vascular/drug effects , Fibrinolytic Agents/pharmacology , Heparin/pharmacology , Heparitin Sulfate/pharmacology , Animals , Anticoagulants/chemistry , Anticoagulants/metabolism , Cattle , Crustacea , Endothelium, Vascular/cytology , Fibrinolytic Agents/chemistry , Fibrinolytic Agents/metabolism , Glycosaminoglycans/metabolism , Glycosaminoglycans/pharmacology , Heparin/chemistry , Heparin/metabolism , Heparin, Low-Molecular-Weight/chemistry , Heparin, Low-Molecular-Weight/metabolism , Heparin, Low-Molecular-Weight/pharmacology , Heparitin Sulfate/biosynthesis , Humans , Structure-Activity Relationship , Tuna
3.
Biochim Biophys Acta ; 1475(3): 287-94, 2000 Jul 26.
Article in English | MEDLINE | ID: mdl-10913828

ABSTRACT

Sulfated glycosaminoglycans were isolated from 23 species of 13 phyla of invertebrates and characterized by their electrophoretic migration in three different buffer systems coupled with enzymatic degradation using bacterial heparinase, heparitinases and chondroitinase AC. Heparan sulfate is a ubiquitous compound present in all species analyzed whereas chondroitin sulfate was present in 20 species and heparin-like compounds in 12 species of the invertebrates. The heparin-like compounds were purified from the echinoderm Mellita quinquisperforata (sand dollar) and the crustacean Ucides cordatus (crab) with anticoagulant activities of 60 and 52 IU/mg, respectively. Degradation of these heparins with heparinase produced significant amounts of the trisulfated disaccharide typical of mammalian heparins. This was confirmed by 13C-NMR spectroscopy of the crab heparin. An updated phylogenetic tree of the distribution of sulfated glycosaminoglycans in the animal kingdom is also presented.


Subject(s)
Glycosaminoglycans/analysis , Invertebrates/chemistry , Animals , Anticoagulants/analysis , Chondroitin Lyases , Chondroitin Sulfates/analysis , Crustacea/chemistry , Echinodermata/chemistry , Electrophoresis, Agar Gel , Glycosaminoglycans/chemistry , Glycosaminoglycans/isolation & purification , Heparin/analysis , Heparin Lyase , Heparitin Sulfate/analysis , Magnetic Resonance Spectroscopy , Polysaccharide-Lyases , Sulfates/analysis
SELECTION OF CITATIONS
SEARCH DETAIL