ABSTRACT
The selection of an appropriately-sized tracheal tube is of critical importance in paediatric patients to reduce both the risk of subglottic stenosis from a tracheal tube that is too large, and inadequate ventilation or poor end-tidal gas monitoring from a tracheal tube that is too small. Age formulae are widely used, but known to be unreliable, often resulting in a need to change the tracheal tube. Previous work has shown that the length of the middle finger and the internal diameter can both be used to guide depth of tracheal tube insertion. Therefore, we hypothesised that middle finger length may also be related to tube internal diameter. We enrolled children aged up to 12 years presenting to our institution for elective anaesthesia and measured the length of the middle finger on the palmar aspect of the hand. Anaesthetists chose the airway device they felt most appropriate for the procedure, and were unaware of the middle finger measurement. Of 160 patients who were enrolled, 108 were included in the final analysis. We found a linear relationship between uncuffed tracheal tube internal diameter and median middle finger length for each size of tracheal tube. Relationship between middle finger length and cuffed tracheal tube internal diameter was less clear. We propose that the formula: 'middle finger length (cm) (round up to nearest 0.5) = internal diameter of uncuffed tracheal tube (mm)' may be an improvement compared with age formulae for selecting uncuffed tracheal tubes in children, although this requires formal testing.
Subject(s)
Fingers/anatomy & histology , Intubation, Intratracheal/instrumentation , Aging/pathology , Anthropometry/methods , Child , Child, Preschool , Equipment Design , Equipment Safety , Female , Humans , Infant, Newborn , MaleABSTRACT
INTRODUCCIÓN: La esplenectomía total en la drepanocitosis se relaciona con riesgo de infecciones sobreagudas y con aumento de la incidencia de otros eventos, lo que no se ha comunicado en pacientes con esplenectomía parcial. En este estudio se caracterizó a los pacientes con drepanocitosis y esplenectomía parcial, y se comparó el comportamiento clínico y de laboratorio con los pacientes no esplenectomizados. MATERIAL Y MÉTODOS: Se estudió a 54 pacientes con drepanocitosis sometidos a esplenectomía parcial durante la edad pediátrica, desde 1986 hasta el año 2011, en el Instituto de Hematología e Inmunología. Se compararon con 54 pacientes no esplenectomizados seleccionados por muestreo aleatorio con características similares. RESULTADOS: La esplenectomía parcial se realizó a una edad media de 4,1 años, con una frecuencia mayor en la anemia drepanocítica (70,4%) y su causa más común fue la crisis de secuestro esplénico recurrente. Las complicaciones posoperatorias más frecuentes fueron: fiebre de origen desconocido (14,8%) y síndrome torácico agudo (11,1%). Después de la esplenectomía, aumentaron significativamente los leucocitos, neutrófilos y plaquetas; estos 2 últimos parámetros se mantuvieron elevados de manera significativa cuando se compararon con los pacientes no esplenectomizados. No hubo diferencias en la incidencia de los eventos clínicos, excepto el secuestro hepático, que fue más frecuente en los esplenectomizados. CONCLUSIÓN: La esplenectomía parcial constituyó un proceder seguro en los pacientes con drepanocitosis. No hubo diferencias en el cuadro clínico entre los niños esplenectomizados y los no esplenectomizados, salvo la mayor frecuencia de crisis de secuestro hepático en los primeros
INTRODUCTION: Total splenectomy in sickle cell disease is related to a high risk of fulminant sepsis and increased incidence of other events, which have not been reported in patients with partial splenectomy. In this study we examined the patients with sickle cell disease and partial splenectomy and compared the clinical and laboratory results with non-splenectomized patients. MATERIAL AND METHODS: We studied 54 patients with sickle cell disease who underwent partial splenectomy in childhood from 1986 until 2011 at the Institute of Hematology and Immunology. They were compared with 54 non-splenectomized patients selected by random sampling with similar characteristics. RESULTS: Partial splenectomy was performed at a mean age of 4.1 years, with a higher frequency in homozygous hemoglobin S (70.4%), and the most common cause was recurrent splenic sequestration crisis. The most common postoperative complications were fever of unknown origin (14.8%) and acute chest syndrome (11.1%). After splenectomy there was a significant increase in leukocytes, neutrophils, and platelets, the latter two parameters remained significantly elevated when compared with non-splenectomized patients. There was no difference in the incidence of clinical events, except hepatic sequestration, which was more common in splenectomized patients. CONCLUSION: Partial splenectomy was a safe procedure in patients with sickle cell disease. There were no differences in the clinical picture in children splenectomized and non-splenectomized except the greater frequency of hepatic sequestration crisis in the first group
Subject(s)
Humans , Male , Female , Child , Adolescent , Young Adult , Child, Preschool , Anemia, Sickle Cell/complications , Splenic Diseases/etiology , Splenic Diseases/surgery , Splenectomy/methods , Retrospective StudiesABSTRACT
INTRODUCTION: Total splenectomy in sickle cell disease is related to a high risk of fulminant sepsis and increased incidence of other events, which have not been reported in patients with partial splenectomy. In this study we examined the patients with sickle cell disease and partial splenectomy and compared the clinical and laboratory results with non-splenectomized patients. MATERIAL AND METHODS: We studied 54 patients with sickle cell disease who underwent partial splenectomy in childhood from 1986 until 2011 at the Institute of Hematology and Immunology. They were compared with 54 non-splenectomized patients selected by random sampling with similar characteristics. RESULTS: Partial splenectomy was performed at a mean age of 4.1 years, with a higher frequency in homozygous hemoglobin S (70.4%), and the most common cause was recurrent splenic sequestration crisis. The most common postoperative complications were fever of unknown origin (14.8%) and acute chest syndrome (11.1%). After splenectomy there was a significant increase in leukocytes, neutrophils, and platelets, the latter two parameters remained significantly elevated when compared with non-splenectomized patients. There was no difference in the incidence of clinical events, except hepatic sequestration, which was more common in splenectomized patients. CONCLUSION: Partial splenectomy was a safe procedure in patients with sickle cell disease. There were no differences in the clinical picture in children splenectomized and non-splenectomized except the greater frequency of hepatic sequestration crisis in the first group.
Subject(s)
Anemia, Sickle Cell/complications , Splenectomy/methods , Splenic Diseases/etiology , Splenic Diseases/surgery , Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Male , Retrospective Studies , Young AdultABSTRACT
The toxic and genotoxic potential of Cadmium (CdCl(2)) were evaluated by the micronucleus test (MNT) and comet assay (CA) using amphibian larvae (Xenopus laevis and Pleurodeles waltl). Acute toxicity results showed that Cd is toxic to Xenopus larvae exposed from 2 to 50 mg/L and to Pleurodeles from 5 to 50 mg/L, depending on the nature of the water (reconstituted water containing mineral salts or mineral water MW (Volvic)). The MNT results obtained in MW showed that Cd (2 mg/L) is genotoxic to Xenopus, whereas it was not genotoxic to Pleurodeles at all concentrations tested. The CA established that the genotoxicity of Cd to Xenopus and Pleurodeles larvae depends on the concentration, the exposure times, and the comet parameters (Tail DNA, ETM, OTM, and TL). The CA and MNT results were compared for their ability to detect genotoxic effects, considering the concentrations of Cd applied and the exposure time. The CA showed Cd to be genotoxic from the first day of exposure. In amphibians, the CA appears to be a sensitive and suitable method for detecting genotoxicity such as that caused by Cd.
Subject(s)
Cadmium/toxicity , Pleurodeles/embryology , Animals , Cadmium/administration & dosage , Comet Assay , Larva/drug effects , Larva/genetics , Micronucleus Tests , Pleurodeles/genetics , Toxicity Tests, Acute , Xenopus laevisABSTRACT
Captan (N-trichloromethylthio-4-cyclohexene-1,2-dicarboximide) is a fungicide used to inhibit the growth of many types of fungi on plants used as foodstuffs. The toxic and genotoxic potentials of captan were evaluated with the micronucleus test (MNT; AFNOR,2000) and the comet assay (CA) using amphibian larvae (Xenopus laevis and Pleurodeles waltl). Acute toxicity results showed that captan was toxic (1) to Xenopus larvae exposed to from 2 mg/L to 125 or 62.5 microg/L, depending on the nature of the water [reconstituted water containing mineral salts or mineral water (MW; Volvic, Danone, France)] and (2) to Pleurodeles exposed to from 2 mg/L to 125 microg/L in both types of water. The MNT results obtained in MW showed that captan (62.5 microg/L) was genotoxic to Xenopus but not genotoxic to Pleurodeles at all concentrations tested. CA established that the genotoxicity of captan to Xenopus and Pleurodeles larvae depended on the concentration, the exposure times, and the comet parameters (tail DNA, TEM, OTM, and TL). The CA and MNT results were compared for their ability to detect DNA damage at the concentrations of captan and the exposure times applied. CA showed captan to be genotoxic from the first day of exposure. In amphibians, CA appears to be a sensitive and suitable method for detecting genotoxicity such as that caused by captan.
Subject(s)
Amphibians/embryology , Captan/toxicity , Pleurodeles/embryology , Soil Pollutants/toxicity , Water Pollutants, Chemical/toxicity , Xenopus laevis/embryology , Animals , Comet Assay/methods , DNA Damage , Environmental Monitoring , Fungicides, Industrial/toxicity , Larva/drug effects , Larva/growth & development , Micronucleus Tests/methods , Time FactorsABSTRACT
The management of contaminated soils and wastes is a matter of considerable human concern. The present study evaluates the genotoxic potential of aqueous extracts of two soils (leachates) and of bottom ash resulting from municipal solid waste incineration (MSWIBA percolate), using amphibian larvae (Xenopus laevis). Soil A was contaminated by residues of solvents and metals and Soil B by polycyclic aromatic hydrocarbons and metals. MSWIBA was predominantly contaminated by metals. Two genotoxic endpoints were analysed in circulating erythrocytes taken from larvae: clastogenic and/or aneugenic effects (micronucleus induction) after 12 days of exposure and DNA-strand-breaking potency (comet assay) after 1 and 12 days of exposure. In addition, in vitro bacterial assays (Mutatox and Ames tests) were carried out and the results were compared with those of the amphibian test. Physicochemical analyses were also taken into account. Results obtained with the amphibians established the genotoxicity of the aqueous extracts and the comet assay revealed that they were genotoxic from the first day of exposure. The latter test could thus be considered as a genotoxicity-screening tool. Although genotoxicity persisted after 12 days' exposure, DNA damage decreased overall between days 1 and 12 in the MSWIBA percolate, in contrast to the soil leachates. Bacterial tests detected genotoxicity only for the leachate of soil A (Mutatox). The results confirm the ecotoxicological relevance of the amphibian model and underscore the importance of bioassays, as a complement to physico-chemical data, for risk evaluation.
Subject(s)
Incineration , Industrial Waste/adverse effects , Soil Pollutants/toxicity , Aliivibrio fischeri/drug effects , Aliivibrio fischeri/metabolism , Animals , Comet Assay , DNA Damage , Environmental Monitoring , Erythrocytes/drug effects , Female , Industrial Waste/analysis , Larva/drug effects , Luminescent Measurements , Male , Micronucleus Tests , Salmonella typhi/drug effects , Salmonella typhi/growth & development , Soil Pollutants/analysis , Water Movements , Xenopus laevisABSTRACT
Management of contaminated dredged sediments is a matter of great human concern. The present investigation evaluates the genotoxic potential of aqueous extracts of five sediments from French channels (draining water from dredged sediments), using larvae of the frog Xenopus laevis. Two genotoxic endpoints were analyzed in larvae: clastogenic and/or aneugenic effects (micronucleus induction after 12 d of exposure) and DNA-strand breaking potency (comet assay after 1 and 12 d of exposure) in the circulating blood. Additionally, in vitro bacterial assays (Microtox and Ames tests) were carried out and the results were compared with those obtained with larvae. Physicochemical analyses were also taken into account. Analytical analyses highlighted in the five draining waters a heavy load of contaminants such as metals and hydrocarbons. The results obtained with the micronucleus test established the genotoxicity of three draining waters. The comet assay showed that all 5 draining waters were genotoxic after 1 d of exposure. Although 3 of them were still genotoxic after 12 d of exposure, DNA damage globally decreased between d 1 and 12. The comet assay can be considered as a genotoxicity-screening tool. Data indicate that both tests should be used in conjunction in Xenopus. Bacterial tests (Ames) revealed genotoxicity for only one draining water. The results confirm the relevance of the amphibian model and the need to resort to bioassays in vivo such as the Xenopus micronucleus and comet assays for evaluation of the ecotoxicological impact, an essential complement to the physicochemical data.
Subject(s)
Environmental Monitoring/methods , Water Pollutants/toxicity , Animals , Comet Assay , France , Larva/drug effects , Micronucleus Tests , Mutagenicity Tests , Xenopus laevisABSTRACT
The present investigation explored the potential use of the comet assay (CA) as a genotoxicity test in the amphibian Xenopus laevis and compared it with the French standard micronucleus test (MNT). Benzo[a]pyrene (B[a]P), methyl methanesulfonate (MMS), and ethyl methanesulfonate (EMS) were used as model compounds for assessing DNA damage. Damage levels were measured as DNA strand breaks after alkaline electrophoresis of nuclei isolated from larval amphibian erythrocytes using the CA in order to establish a positive control for further ecotoxicological investigations. The results led to the selection of MMS as a positive control on the basis of the higher sensitivity of Xenopus laevis to this compound. The CA and MNT were compared for their ability to detect DNA damage with the doses of chemical agents and exposure times applied. EMS and MMS were shown to increase micronucleus and DNA strand break formation in larval erythrocytes concurrently. However, B[a]P increased micronucleus formation but not that of DNA strand breaks. Time-dose experiments over 12 days of exposure suggest that the CA provides an earlier significant response to genotoxicants than does the MNT. In Xenopus the CA appears to be a sensitive and suitable method for detecting genotoxicity like that caused by EMS and MMS. It can be considered a genotoxicity-screening tool. The results for B[a]P show that both tests should be used in a complementary manner on Xenopus.
Subject(s)
Benzo(a)pyrene/toxicity , Control Groups , Ethyl Methanesulfonate/toxicity , Methyl Methanesulfonate/toxicity , Mutagens/toxicity , Xenopus laevis/embryology , Animals , Comet Assay , DNA Damage , Female , Male , Micronucleus Tests , Reference ValuesABSTRACT
The green alga, Scenedesmus subspicatus was exposed for 7 days to a series of PAHs (polyaromatic hydrocarbons) of increased molecular weight from two to five rings [naphthalene (Nap), anthracene (Ant), phenanthrene (Phe), pyrene (Pyr) and benzo(a)pyrene (BaP)]. The toxicity measured as population growth inhibition by individual PAH to the S. subspicatus followed the order: BaP>Pyr>Ant>Phe>Nap. These results confirmed that the toxicity potential of PAHs seems to be strongly influenced by their physico-chemical properties (aqueous solubility, K(ow), coefficient of volatilization, etc.) and the conditions of algae culture (light, presence of nitrate ions, etc.). Consequently, Nap, Phe and Ant having low k(ow) values and low coefficient of volatilization values were less toxic than BaP with the highest k(ow) value, indicating for example why Nap with the lowest EC(50) value was nearly 2 x 10(5) times lower than that of BaP. Moreover, nitrate ions seemed to act directly on the degree of hydroxylated radical reactivity of PAHs, since BaP always remained the most toxic of the compounds tested. The results were also agreed with the QSAR model for toxicity prediction of PAHs to many aquatic organisms.
Subject(s)
Petroleum , Polycyclic Aromatic Hydrocarbons/toxicity , Scenedesmus , Water Pollutants, Chemical/toxicity , Geologic Sediments/chemistry , Polycyclic Aromatic Hydrocarbons/chemistry , Quantitative Structure-Activity Relationship , VolatilizationABSTRACT
Artificial aqueous samples (eluates, percolates, immersion waters) were obtained from contaminated soils and stabilized industrial wastes. The toxicity and genotoxicity of these aqueous fractions have been evaluated in vivo in the aquatic larvae of the amphibian Xenopus laevis. Four biotests have been applied: a test of subchronic toxicity and three biomakers: (1) measurement of the activity of ethoxyresorufine-o-dealkylase in the liver, (2) detection of DNA adducts in the liver and the blood, and (3) measurement of the rate of micronuclei in the erythrocytes. Biological datas were completed through a chemical analysis. The main conclusions of this study are: The importance of integrating different toxicity criterias into a biological battery (phenotypic and genotypic criterias). Some aqueous extracts did not seem to be very toxic, whereas their genotoxic effects were rather significant [e.g. the stabilized Municipal Solid Waste (MSW) ashes]. The importance of coupling together chemical and biological approaches to refine the impact. Actually, some eluates (lixiviation or percolation) coming from polluted soils appeared to be very poorly loaded with pollutants, whereas the toxic and genotoxic impact of these complex matrices were rather noticeable. In addition, when applying the leaching standardized procedure, the hazardous potential of the two analysed soils may be underestimated if the results on percolates and on eluates have been compared. This study highligths the importance of coupling the tools of characterization and preparation of samples to be analysed according to the objectives to be reached.
Subject(s)
Biomarkers/analysis , Cytochrome P-450 CYP1A1/pharmacology , DNA Adducts/analysis , Industrial Waste/adverse effects , Soil Pollutants/toxicity , Animals , Cytochrome P-450 CYP1A1/analysis , Erythrocytes , Larva , Micronucleus Tests , Mutagenicity Tests , Xenopus laevis/growth & developmentSubject(s)
Drosophila Proteins , Lipopolysaccharides/pharmacology , Membrane Glycoproteins/metabolism , Receptors, Cell Surface/metabolism , Animals , B-Lymphocytes/drug effects , B-Lymphocytes/metabolism , Cell Line , Genes, Reporter/genetics , Humans , Molecular Chaperones/genetics , Molecular Chaperones/metabolism , Mutagenesis , NF-kappa B/genetics , NF-kappa B/metabolism , Recombinant Fusion Proteins/metabolism , Toll-Like ReceptorsSubject(s)
Industrial Waste/adverse effects , Micronucleus Tests , Mutagens/toxicity , Pleurodeles , Water Pollutants, Chemical/toxicity , Animals , Erythrocytes/drug effects , France , Fresh Water/analysis , Industrial Waste/analysis , Larva/drug effects , Larva/genetics , Petroleum , Water Pollutants, Chemical/analysisABSTRACT
The accumulation kinetics in the freshwater mollusk Corbicula fluminea were measured for sediment-associated selected polycyclic aromatic hydrocarbons (PAHs), namely, anthracene (Ant), phenanthrene (Phe), pyrene (Pyr), and benzo [a] pyrene (BaP). The desorption from sediment to water was not only related to water solubility, but also depended on structural and physicochemical properties of PAHs (molecular weight, molecular area, octanol/water partition coefficient Kow). Uptake of PAHs by mollusks was very fast in the first 24 h and maximum for low-molecular-weight compounds. Then animal concentrations decreased significantly after the first day for Ant and Phe, after the second day for Pyr, and after the second week for BaP (at this time the BaP concentration was higher than concentrations of the other PAHs). The kinetic data suggest that PAH uptake is related to desorption from sediment particles. Log PAH uptake is inversely related to log Kow. Bioavailability of sediment-sorbed PAHs declined with contact time between sediment and animals. Assimilation from ingested material may be significant for the more strongly sorbed compound (BaP). PAHs with low Kow values become biologically unavailable more rapidly and more efficiently excreted.
Subject(s)
Mollusca/metabolism , Polycyclic Aromatic Hydrocarbons/metabolism , Soil Pollutants/metabolism , Water Pollutants, Chemical/metabolism , Absorption , Animals , Anthracenes/metabolism , Benzo(a)pyrene/metabolism , Geologic Sediments , Linear Models , Phenanthrenes/metabolism , Pyrenes/metabolismABSTRACT
The capacity of the newt to metabolize benzo(a)pyrene in vivo was investigated qualitatively and quantitatively: metabolism was found to be rapid. Treatment of bile with beta-glucuronidase and aryl-sulfatase released high proportions of diols and quinones. 3-Methylcholanthrene treatment shortened the elimination half-life of benzo(a)pyrene which was about three times shorter than the half-life found for non-3-methylcholanthrene-pretreated animals. Thus, a greater proportion of benzo(a)pyrene was converted into water-soluble products.
Subject(s)
Benzo(a)pyrene/pharmacokinetics , Environmental Pollutants/pharmacokinetics , Pleurodeles/metabolism , Animals , Bile/chemistry , Bile/metabolism , Biotransformation , Chromatography, High Pressure Liquid , Half-Life , Injections, Intraperitoneal , Liver/chemistry , Liver/drug effects , Liver/metabolism , Methylcholanthrene/pharmacology , RadiometryABSTRACT
The micronucleus test using erythrocytes of Pleurodeles waltl larvae (Amphibia, Salamandridae) was used to detect the possible genotoxicity of four polycyclic aromatic hydrocarbons (naphthalene, anthracene, phenanthrene and benzo[a]pyrene), which represent a major fraction of crude oil. Larvae were reared in water containing the test compound and the levels of micronucleated red blood cells were compared with those found in larvae reared in control water. The results are compared with published data from other tests used to evaluate the clastogenic or mutagenic properties of compounds. The results obtained confirm that benzo[a]pyrene has a strong genotoxic potential, whereas the genotoxicity of naphthalene is weak; in contrast, anthracene and phenanthrene gave a negative response.
Subject(s)
Fuel Oils/toxicity , Micronucleus Tests/methods , Mutagens/toxicity , Polycyclic Compounds/toxicity , Animals , Anthracenes/toxicity , Benzo(a)pyrene/toxicity , Erythrocytes/drug effects , Erythrocytes/ultrastructure , Larva , Naphthalenes/toxicity , Phenanthrenes/toxicity , PleurodelesABSTRACT
The genotoxic potential of various waste waters has been evaluated in a micronucleus test using amphibian larvae. Genotoxicity was detected after dilution, in waste water from tanneries and from various petrochemical industries. Further studies have shown that sample treatment used for in vitro testing may affect the genotoxic response. Sterilization by gamma irradiation lowered genotoxic activity. Furthermore, microfiltration of effluent and extraction of organic micropollutants on XAD-4 resins, lead to the preparation of extracts which are not fully representative of the initial water sample. Testing of concentrates, as required for in vitro studies, will limit the scope of a survey to that part of the organic matter that can be recovered by concentration techniques. Many of the problems encountered in in vitro genotoxicity studies of waters, may be circumvented with direct testing on aquatic organisms. Thus, there is no need to concentrate or sterilise a sample. The tests can be carried out with intact animals, thus taking into account uptake and elimination, internal transport and metabolism. Finally, in vivo test-systems, such as the Newt Micronucleus Test, are more relevant to eukaryotes than bacterial assays and are suitable to assess the real impact of genotoxins discharged in the aquatic environment.
Subject(s)
Industrial Waste/adverse effects , Micronucleus Tests/methods , Salamandridae , Animals , Larva/drug effects , Petroleum , TanningABSTRACT
A model micronucleus test system using peripheral blood erythrocytes from larvae of Pleurodeles waltl is described. The most suitable larval stage for testing chemical treatments was determined. Larvae were reared in water containing one of the 4 compounds: benzo[a]pyrene (BaP), ethyl methanesulphonate (EMS), diethyl sulphate (DES) and N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG). Response curves as a function of treatment duration over a period of 16 days were plotted for 3 different concentrations of the 4 compounds in order to optimize conditions for a low dose micronucleus test. This model can be used as a monitoring system for the detection of fresh water pollution and can also be employed for clastogen screening of chemical compounds. The test is sensitive, reliable and easy to use.
Subject(s)
Cell Nucleus/ultrastructure , Mutagenicity Tests/methods , Pleurodeles/blood , Salamandridae/blood , Water Pollutants/analysis , Animals , Benzo(a)pyrene/toxicity , Cell Nucleus/drug effects , Diethylstilbestrol/toxicity , Dose-Response Relationship, Drug , Erythrocytes/drug effects , Erythrocytes/ultrastructure , Ethyl Methanesulfonate/toxicity , Methylnitronitrosoguanidine/analogs & derivatives , Methylnitronitrosoguanidine/toxicityABSTRACT
The polymorphism of three enzymes [lactate dehydrogenase B (LDH-B), glucose-6-phosphate dehydrogenase, (G6PDH), and peptidase-1 (PEP-1)] in Pleurodeles waltl has allowed the expression of the corresponding loci to be followed during the development of spawnings arising from various crosses. A maternal effect lasting up to the late tail-bud stage (approx. stage 28) was shown for PEP-1. A similar situation was observed for LDH-B and G6PDH. The embryonic alleles present retarded expression: from about stage 28 for PEP-1 and G6PDH and from about stages 22 to 24 (the young tail-bud stage) for LDH-B.
Subject(s)
Gene Expression Regulation , Glucosephosphate Dehydrogenase/biosynthesis , L-Lactate Dehydrogenase/biosynthesis , Peptide Hydrolases/biosynthesis , Pleurodeles/metabolism , Salamandridae/metabolism , Animals , Cytoplasm/physiology , Female , Glucosephosphate Dehydrogenase/genetics , L-Lactate Dehydrogenase/genetics , Metamorphosis, Biological , Ovum/physiology , Pleurodeles/embryology , Pleurodeles/geneticsABSTRACT
The existence of four peptidases was demonstrated by starch gel electrophoresis in Pleurodeles waltlii: PEP-1, PEP-2, PEP-3, and PEP-4. Peptidases-3 and -4 are monomorphic, and peptidases-1 and -2 are polymorphic. The heredity of the polymorphisms was studied using individuals arising from crosses or of gynogenetic origin. Peptidase-1 is dimeric; its polymorphism depends on a pair of codominant alleles, Pep-1A and Pep-1B, which are situated on the Z and W sex chromosomes, respectively, in close proximity to, or even within, the sex differential segment. As the differential segment is very close to the centromere, the PEP-1 locus therefore also appears to be closely linked to it. Expression of the PEP-1 locus was shown to be independent of the sex hormone environment. This locus is the first case reported in amphibians of an enzyme marker linked to the genetic sex. It allows the sex of PLeurodeles to be determined before they reach sexual maturity. Peptidase-2 is monomeric. Its polymorphism depends on a pair of codominant alleles on an autosomal PEP-2 locus. The high proportion of heterozygous animals in the gynogenetic offspring of females heterozygous for the PEP-2 locus indicates segregation which is independent of the centromere. Analysis of the offspring of doubly heterozygous females (i.e., for two of the loci--LDH-B, G6PDH, PEP-1, and PEP-2) shows that the four loci are independent.