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1.
ChemSusChem ; : e202401055, 2024 Jun 25.
Article in English | MEDLINE | ID: mdl-38924618

ABSTRACT

The development of green and environmentally friendly synthesis methods of electrocatalysts is a crucial aspect in decarbonizing energy generation. In this study, eco-friendly mechanochemical synthesis of perovskite metal oxide-carbon black composites is proposed using different conditions and additives such as KOH. Furthermore, the optimization of ball milling conditions, including time and rotational speed, is studied. The mechanochemical synthesis in solid-state conditions without additives produces electrocatalysts that exhibit the highest bifunctional electrochemical activity towards both oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). Moreover, this synthesis demonstrates a lower Environmental Impact Factor (E-factor), indicating its greener nature, and due to its simplicity, it has a great potential for scalability. The obtained bifunctional electrocatalysts have been tested in a rechargeable zinc-air battery (ZAB) for 22 h with similar performance compared to the commercial catalyst (Pt/C) at significantly lower cost. These promising findings are attributed to the enhanced interaction between the perovskite metal oxide and carbon material and the improved dispersion of the perovskite metal oxide on the carbon materials.

2.
PLoS One ; 18(11): e0291112, 2023.
Article in English | MEDLINE | ID: mdl-37967079

ABSTRACT

Pain is an unpleasant experience that we will all experience in some form over the course of our lives, with chronic pain affecting a significant proportion of the global population. Given these circumstances, this study investigates whether pain is a legitimated phenomenon and considers the processes involved in the creation of such a status. This is an exploratory investigation based on semi-structured interviews with people suffering from chronic pain as a consequence of physical, psychological, emotional, or social circumstances. Our principal objective is to explore the fundamental elements of legitimacy and the processes that bring it into being-i.e., to understand how it is socially constructed. The main finding, however, is that many sufferers of chronic physical, psychiatric/psychological, emotional and social pain perceive that their pain is not considered legitimate when no clear cause can be identified, when the pain prevents them from developing the norms imposed by social roles or when it inhibits them to make a productive contribution to the society in which they live. This is generally due to the disruptive aspect of pain and its impact on the social structure, specifically on productivity, which nowadays constitutes a key element in the legitimation of any social phenomenon.


Subject(s)
Chronic Pain , Humans , Emotions
3.
Vet Med Sci ; 9(3): 1031-1035, 2023 05.
Article in English | MEDLINE | ID: mdl-37029758

ABSTRACT

A 10-month-old female domestic shorthaired (DSH) cat was presented with peracute respiratory problems. Physical examination revealed dyspnoea, tachypnoea, cyanosis, weak pulse and bradycardia. Auscultation showed pulmonary crepitation and attenuated heart sounds and a pansystolic grade V/VI murmur. The electrocardiogram showed atrioventricular dissociation identified as third-degree sinoatrial block. X-rays showed increased density in the ventral and middle zones of the thorax and loss of definition of the cardiac silhouette and increased diffuse radiographic density of the entire abdomen. Echocardiography revealed dilatation of the right atrium and concentric biventricular hypertrophy. A type 1 persistent truncus arteriosus was diagnosed at necropsy. This is the first case report of this type of arrhythmia in a cat with persistent truncus arteriosus, and its relationship with the described congenital cardiac anomaly is discussed.


Subject(s)
Cat Diseases , Truncus Arteriosus, Persistent , Female , Cats , Animals , Truncus Arteriosus, Persistent/diagnosis , Truncus Arteriosus, Persistent/veterinary , Echocardiography , Electrocardiography , Diagnosis, Differential , Heart Block/diagnosis , Heart Block/veterinary , Cat Diseases/diagnostic imaging
4.
Gac méd espirit ; 25(1)ene.-abr. 2023.
Article in Spanish | CUMED | ID: cum-79326

ABSTRACT

Fundamentación:En el período de gestación el organismo de la mujer sufre modificaciones, transformaciones fisiológicas y psicológicas, además de cambios en los tejidos bucales y cambios de conducta que pueden iniciar enfermedades bucodentales o agravar las ya establecidas.Objetivo:Evaluar la efectividad del programa educativo “Sonrisas desde el vientre” en embarazadas del Hogar Materno Isabel María de Valdivia en Sancti Spíritus.Metodología:Se realizó un estudio preexperimental, con diseño antes y después en el período de enero a junio del 2022, la población estuvo constituida por 20 embarazadas ingresadas en el Hogar Materno Isabel María de Valdivia en Sancti Spíritus. Se utilizaron métodos del nivel teórico, empírico (encuesta) y estadísticos. Se midió la variable conocimiento sobre salud bucodental.Resultados:Los conocimientos antes de la aplicación de programa educativo eran insuficientes (65 porciento), después de aplicado el programa educativo el 75 porciento de las embarazadas presentó conocimientos suficientes.Conclusiones:Al culminar la implementación del programa educativo “Sonrisas desde el vientre”, se logró un nivel de conocimiento suficiente sobre salud bucodental en la tercera parte de las embarazadas de este hogar materno.[AU]


Subject(s)
Humans , Pregnant Women/education , Dental Health Surveys/methods , Health Education, Dental , Oral Health/education , Education, Dental/methods , Health Education
5.
J Colloid Interface Sci ; 630(Pt B): 269-280, 2023 Jan 15.
Article in English | MEDLINE | ID: mdl-36327729

ABSTRACT

Zn-air batteries (ZABs) are promising electrochemical devices to store energy. Metal oxide perovskites mixed with carbon materials are highlighted as interesting materials for this application because of their appropriate bifunctional performance in oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). The interaction between both components of the electrocatalyst is important in the bifunctional electrocatalytic activity, and the mixing method plays an important role in this interaction. Then, different mixing methods have been studied in this work (ball-milling, mortar and manual shaking). The use of different physicochemical techniques such as temperature programmed desorption (TPD), temperature programmed reduction (TPR) and X-ray photoelectron spectroscopy (XPS) in the materials characterization, allows us to conclude that the mixing method strongly influences the particle size and the interaction between both components, which determine the final electrocatalytic activity. The materials prepared by ball-milling displayed the best performance. Herein, the experimental conditions were optimized to obtain electrocatalysts with enhanced electrocatalytic activity for ORR and OER. Low rotating speed, air atmosphere and low ball-milling time generate electrocatalysts with a small nanoparticle size, more homogeneous and with a higher interaction between both components, which enhances electron transfer, and consequently, the overall oxygen-involved reactions. The best electrocatalyst obtained was studied as air-electrode in a Zn-air battery and it was compared to a commercial Pt/C electrocatalyst, obtaining higher cyclability (55.2 vs 51.7 %) for 30 h, and higher energy density at 5 mA/cm2 (764 mAh/g vs 741 mAh/g).

6.
Gac. Med. Espirit ; 24(3): 1-9, 2022.
Article in Spanish | CUMED | ID: cum-79319

ABSTRACT

Fundamentación: Las embarazadas constituyen un grupo poblacional vulnerable a varios problemas de salud,entre ellos los del componente bucal, debido en parte al desconocimiento de los factores que los propician y las modificaciones que ocurren en este periodo.Objetivo: Identificar el nivel de conocimientos, actitudes, prácticas y autopercepción de salud bucal enembarazadas del Consultorio San Pedro en Trinidad.Metodología: Se realizó un estudio observacional descriptivo de corte transversal de septiembre del 2020 afebrero del 2021. Se seleccionaron las 57 pacientes embarazadas del Consultorio Médico de las Familias de SanPedro, Trinidad. Se estudiaron las variables: edad, nivel de conocimiento, actitudes, prácticas y autopercepciónde salud bucal.Resultados: Se constató un insuficiente nivel de conocimientos sobre salud bucal en el 47.4 porciento de lasembarazadas, el 70.2 porciento con actitud desfavorable, el 63.2 porciento con prácticas deficientes y el 68.4 porciento con bajaautopercepción sobre salud bucal.Conclusiones: Menos de la mitad de las embarazadas presentó un bajo nivel de conocimientos sobre saludbucal y más de la mitad, una actitud desfavorable, prácticas deficientes y baja autopercepción sobre salud bucal. [AU]


Subject(s)
Humans , Oral Health/education , Attitude , Pregnancy
7.
Food Chem ; 337: 127786, 2021 Feb 01.
Article in English | MEDLINE | ID: mdl-32795861

ABSTRACT

The dehydration process is a prerequisite to preserve saffron for a long time. According to this process, saffron shows differences in the main compounds responsible for its quality (colour, taste, aroma, and flavonol content). At present, the freeze-drying method obtains dried products with the highest quality. Viruses can modify the physiology and metabolism of plants, being able to affect the activities of several enzymes. For this reason, the main compounds of saffron have been analyzed under two different dehydrating processes, freeze-drying and dark-drying, considering their infection status with the Saffron latent virus (SaLV). Results showed that the picrocrocin and safranal content enables to differ dark-dried samples from freeze-dried ones. Besides, the kaempferol-3-O-sophoroside-7-O-glucoside content allows differentiating between SaLV-infected (SaLV+) and uninfected (SaLV-) saffron samples. Moreover, our data suggest that the freeze-drying would decrease crocins content, and dark-drying can nullify the adverse effect of SaLV on crocins content.


Subject(s)
Crocus/virology , Desiccation/methods , Phytochemicals/analysis , Virus Diseases/epidemiology , Carotenoids/analysis , Carotenoids/metabolism , Crocus/classification , Crocus/metabolism , Cyclohexenes/analysis , Cyclohexenes/metabolism , Glucosides/analysis , Glucosides/metabolism , Iran , Kaempferols/analysis , Kaempferols/metabolism , Phytochemicals/metabolism , Plant Diseases , Prevalence , Terpenes/analysis , Terpenes/metabolism
8.
Food Chem ; 221: 838-843, 2017 Apr 15.
Article in English | MEDLINE | ID: mdl-27979282

ABSTRACT

The aim of this work was a comparison of the ISO 3632 (2011) method and an HPLC-DAD method for safranal quantity determination in saffron. Samples from different origins were analysed by UV-vis according to ISO 3632 (2011) and by HPLC-DAD. Both methods were compared, and there was no correlation between the safranal content obtained by UV-vis and HPLC-DAD. An over-estimation in the UV-vis experiment was observed, which was related to the cis-crocetin esters content, as well as other compounds. The results demonstrated that there was no relationship between ISO quality categories and safranal content using HPLC-DAD. Therefore, HPLC-DAD might be preferable to UV-vis for determining the safranal content and the classification of saffron for commercial purposes. In addition, HPLC-DAD was adequate for determining the three foremost parameters that define the quality of saffron (crocetin esters, picrocrocin and safranal); therefore, this approach could be included in the ISO 3632 method (2011).


Subject(s)
Chromatography, High Pressure Liquid , Crocus/chemistry , Cyclohexenes/analysis , Food Analysis/methods , Terpenes/analysis , Ultraviolet Rays , Carotenoids/analysis , Esters/analysis , Food Quality , Glucosides/analysis , Plant Extracts/chemistry , Vitamin A/analogs & derivatives , Volatile Organic Compounds/analysis
9.
Rev Esp Quimioter ; 30(1): 62-78, 2017 Feb.
Article in Spanish | MEDLINE | ID: mdl-28032738

ABSTRACT

According to published data, prevalence of imported eosinophilia among travellers and immigrants is set between 8% and 28.5%. Etiological diagnosis is often troublesome, and depending on the depth of the study and on the population analyzed, a parasitic cause is identified in 17% to 75.9% of the individuals. Among the difficulties encountered to compare studies are the heterogeneity of the studied populations, the type of data collection (prospective/retrospective) and different diagnostic protocols. In this document the recommendations of the expert group of the Spanish Society of Tropical Medicine and International Health (SEMTSI) for the diagnosis and treatment of imported eosinophilia are detailed.


Subject(s)
Emigrants and Immigrants , Eosinophilia/diagnosis , Eosinophilia/therapy , Travel , Tropical Medicine , Consensus , Eosinophilia/parasitology , Helminthiasis/blood , Helminthiasis/drug therapy , Helminthiasis/parasitology , Humans , Societies, Medical , Spain
10.
Clin Immunol ; 169: 80-84, 2016 Aug.
Article in English | MEDLINE | ID: mdl-27236002
11.
J Agric Food Chem ; 62(32): 8068-74, 2014 Aug 13.
Article in English | MEDLINE | ID: mdl-25075549

ABSTRACT

The aim of this work was to propose a high-performance liquid chromatography with diode array detection (HPLC-DAD) method for determining the three main compounds responsible for determining the quality of saffron (crocetin esters, picrocrocin, and safranal) by preparing an aqueous extract according to the ISO 3632 standard to solve the difficulty that this standard has for aroma and taste determination by ultraviolet-visible spectroscopy. Toward this aim, laboratory-isolated picrocrocin, a safranal standard with a purity of ≥ 88%, trans-crocetin di(ß-D-gentiobiosyl) ester (trans-4-GG) and trans-crocetin (ß-D-glucosyl)-(ß-D-gentiobiosyl) ester (trans-3-Gg) standards, both with a purity of ≥ 99%, and 50 different saffron spice samples from Italy, Iran, Greece, and Spain were used in the intralaboratory validation of the HPLC method. The analytical method proposed was adequate in terms of linearity, selectivity, sensitivity, and accuracy for determining the three foremost parameters that define the quality of saffron using only a saffron solution prepared according to the ISO 3632 standard.


Subject(s)
Crocus/chemistry , Flowers/chemistry , Food Inspection/methods , Food Quality , Spices/analysis , Calibration , Chromatography, High Pressure Liquid , Crocus/growth & development , Cyclohexenes/analysis , Cyclohexenes/chemistry , Flowers/growth & development , Glucosides/analysis , Glucosides/chemistry , Limit of Detection , Mediterranean Region , Molecular Structure , Plant Extracts/chemistry , Reproducibility of Results , Solubility , Spain , Spectrophotometry, Ultraviolet , Stereoisomerism , Terpenes/analysis , Terpenes/chemistry
12.
SEMERGEN, Soc. Esp. Med. Rural Gen. (Ed. impr.) ; 39(5): 252-258, jul.-ago. 2013.
Article in Spanish | IBECS | ID: ibc-113948

ABSTRACT

Objetivo: Describir los resultados de la implantación del programa piloto, en la comunidad de Castilla y León, del cribado de cáncer colorrectal (CCR) mediante la detección de sangre oculta en heces utilizando una prueba inmunológica cuantitativa de aglutinación en látex (TSOHi). Métodos: Población diana de 4.930 personas entre 50-69 a˜nos de la zona básica de salud de Medina del Campo. A los que presentaron TSOHi positivo se les realizó colonoscopia. Se calcularon tasas de participación, positividad, aceptación de colonoscopia, detección de lesiones, porcentajes y valor predictivo positivo (VPP) de la prueba. Resultados: La tasa de participación fue de 2.241 personas (46.33%). Los TSOHi positivos fueron 138 (6,15%). La tasa de aceptación de la colonoscopia fue del 99,27%. Se detectaron 12 pacientes con CCR (el 91,66% en estadios precoces), 42 con adenoma de alto riesgo (AAR) y 34 con adenoma de bajo riesgo (ABR). Las tasas de detección fueron para el CCR de 5,35‰, para el AAR de 18,74‰, para el ABR de 15,17‰ y del 39,26‰ para todo tipo de adenoma. El VPP fue del 8,69% para el CCR, del 30,43% para el AAR y del 24,63% para el ABR. Conclusiones: El programa de detección de CCR es factible en nuestro contexto. Los indicadores del TSOHi son superiores a los de otros estudios realizados con pruebas clásicas. Las altas tasas de detección de CCR y de todo tipo de adenoma justificarían por sí solas el estudio. Estas, junto con la precocidad del diagnóstico de CCR, harían posible anticipar una reducción de la mortalidad (AU)


Objective: To describe the results of implementing a pilot screening program, in the Castilla y León, for colorectal cancer (CRC) with the faecal occult blood test (iFOBT) using a quantitative immunological latex agglutination assay. Methods: The study population included 4930 persons between 50-69 years from the Basic Health Area of Medina del Campo. Colonoscopy was performed on those who had a positive iFOBT. The rates of participation were calculated, positivity, acceptance of colonoscopy, detection of lesions, percentages and predictive positive value (PPV) of the test. Results: A total of 2241 (46.33%) people took part. There were 138 (6.15%) positive iFOBT. The rate acceptance of the colonoscopy was 99.27%. CRC was detected in 12 patients (91.66% in early stages), a high risk adenoma (HRA) in 42, and a low risk adenoma (LRA) in 34. The rates of detection were for CRCwas 5.35‰, 18.74‰ for HRA, 15.17‰ for LRA, and 39.26‰ for all kinds of adenoma. The PPV was 8.69% for CCR, 30.43% for HRA and 24.63% for LRA. Conclusions: The CRC screening program is feasible in our context. The iFOBT indicators are superior to those of other studies performed using the classic test. The high rates of detection of CRC, and all kinds of adenoma would be enough to justify the study. These together with the diagnosis of CRC in the early stages could lead to a reduction of the mortality (AU)


Subject(s)
Humans , Male , Female , Middle Aged , Colorectal Neoplasms/diagnosis , Colorectal Neoplasms/immunology , Early Diagnosis , Immunologic Tests/instrumentation , Immunologic Tests/methods , Immunologic Tests , Predictive Value of Tests , Occult Blood , Colonoscopy/methods , Mass Screening/methods , Colorectal Neoplasms/epidemiology , Colorectal Neoplasms/prevention & control , Urban Population/trends , Primary Health Care/methods , Primary Health Care , Immunologic Tests/trends
13.
Semergen ; 39(5): 252-8, 2013.
Article in Spanish | MEDLINE | ID: mdl-23834975

ABSTRACT

OBJECTIVE: To describe the results of implementing a pilot screening program, in the Castilla y León, for colorectal cancer (CRC) with the faecal occult blood test (iFOBT) using a quantitative immunological latex agglutination assay. METHODS: The study population included 4930 persons between 50-69 years from the Basic Health Area of Medina del Campo. Colonoscopy was performed on those who had a positive iFOBT. The rates of participation were calculated, positivity, acceptance of colonoscopy, detection of lesions, percentages and predictive positive value (PPV) of the test. RESULTS: A total of 2241 (46.33%) people took part. There were 138 (6.15%) positive iFOBT. The rate acceptance of the colonoscopy was 99.27%. CRC was detected in 12 patients (91.66% in early stages), a high risk adenoma (HRA) in 42, and a low risk adenoma (LRA) in 34. The rates of detection were for CRCwas 5.35‰, 18.74‰ for HRA, 15.17‰ for LRA, and 39.26‰ for all kinds of adenoma. The PPV was 8.69% for CCR, 30.43% for HRA and 24.63% for LRA. CONCLUSIONS: The CRC screening program is feasible in our context. The iFOBT indicators are superior to those of other studies performed using the classic test. The high rates of detection of CRC, and all kinds of adenoma would be enough to justify the study. These together with the diagnosis of CRC in the early stages could lead to a reduction of the mortality.


Subject(s)
Adenoma/diagnosis , Colorectal Neoplasms/diagnosis , Early Detection of Cancer , Adenoma/epidemiology , Adenoma/immunology , Aged , Catchment Area, Health , Colonoscopy , Colorectal Neoplasms/epidemiology , Colorectal Neoplasms/immunology , Female , Humans , Latex Fixation Tests , Male , Middle Aged , Pilot Projects , Prevalence , Spain/epidemiology , Urban Health
15.
Farm. hosp ; 37(3): 267-269, mayo-jun. 2013.
Article in Spanish | IBECS | ID: ibc-117396

ABSTRACT

Objetivo: Describir un caso probable de desarrollo de hipertensión pulmonar (HP) secundario al tratamiento con talidomida para la prevención secundaria de sangrado gastrointestinal por angiodisplasias. Case Summary: Mujer de 61 años de edad en tratamiento crónico con acenocumarol debido a prótesis valvular mitral, con ingresos repetidos por sangrado gastrointestinal por presentar angiodisplasias intestinales y anemia ferropénica llegando a ser dependiente de transfusiones. Inició tratamiento como prevención secundaria del sangrado gastrointestinal con talidomida 50 mg diarios. Tras cuatro meses de tratamiento, la paciente alcanzó niveles de hemoglobina estables (12,8 mg/dl) y no volvió a presentar episodios de sangrado, ingresó por presentar aumento de su disnea habitual objetivándose hipertensión pulmonar severa (HP) mediante ecocardiografía que se asoció al tratamiento con talidomida. Se suspendió el tratamiento y mejoró notablemente su situación clínica. Discusión: Diversos casos han sido publicados sobre el tratamiento farmacológico preventivo secundario de la hemorragia digestiva secundaria a angiodisplasias con talidomida con unos resultados favorables. En nuestro caso, la paciente presentó buena respuesta a dicha indicación, pero se tuvo que interrumpir por el empeoramiento significativo de la HP. Existen casos publicados de la asociación de talidomida y HP. La mayoría utilizando una dosis de 100-400 mg diarios. En nuestro caso se desarrolló a dosis de 50 mg. Según la escala Naranjo de probabilidad, se presentó una asociación probable. Conclusiones: Los profesionales de la salud deben tener consciencia del riesgo de desarrollar HP al inicio del tratamiento con talidomida. Han de identificarse los factores de riesgo y patologías que cursen con disfunción endotelial y hacer una valoración más exacta del beneficio-riesgo del tratamiento (AU)


Subject(s)
Humans , Female , Middle Aged , Hypertension, Pulmonary/chemically induced , Thalidomide/adverse effects , Angiodysplasia/drug therapy , Risk Factors
16.
Arch. méd. Camaguey ; 17(1)ene.-feb. 2013. ilus
Article in Spanish | CUMED | ID: cum-55490

ABSTRACT

Aunque la toracotomía ántero lateral fue la primera vía de acceso comointento de una resección pulmonar para los neoplasmas pulmonares, las vías deaccesos posteriores y posterolaterales rápidamente aparecieron como una opción segura. En la década de los años 50 la incisión posterolateral era la de elección. Recientemente, la esternotomía media, así como la incisión de Hemiclamshell (esternotomía longitudinal con prolongación torácica) y la incisión de Clamshell (esternotomía transversa) se comenzaron a utilizar en circunstancias especiales. Objetivos: introducir la esternotomía media en el tratamiento del cáncer del pulmón.M étodos: se realizó la descripción de la técnica de la esternotomía media como vía de acceso para la resección pulmonar por cáncer así como la disección de las estacionesganglionares 1, 2, 3, 4, 5, 6 y 7. Se consultaron los repositorios Ebsco, Medline y Cochrane para la obtención de la bibliografía. Conclusiones: la esternotomía media es una vía segura y útil para la resección pulmonar por cáncer y disección mediastinal, lacual brinda además una clasificación patológica más exacta que las toracotomías convencionales al permitir el acceso a las zonas ganglionares superiores, aórticas y subcarinales de una forma bilateral según corresponda (AU)


In spite of the fact that anterolateral thoracotomy was the first approach as an attempt to conduct a pulmonary resection for pulmonary neoplasms,posterior and posterolateral approaches rapidly became a safe option. In the 50s, posterolateral incision was the chosen option. Recently, specialists have started to use medial sternotomy, as well as Hemiclamshell's incision (longitudinal sternotomy withthoracic prolongation) and Clamshel's incision (transverse sternotomy) and they are carried out in particular circumstances. Objectives: introduce medial sternotomy for treating lung cancer. Method: the technique of medial sternotomy as an approach toconduct a pulmonary resection for cancer and the dissection of ganglial stations 1, 2, 3, 4, 5, 6, and 7, were described. Ebsco, Medline and Cochrane were consulted to obtain references. Conclusions: medial sternotomy is a safe and useful way to conduct pulmonary resection for cancer and mediastinal dissection, which also provides a more exact pathological classification than conventional thoracotomies, because it allows the approach to the superior, aortic, and subcarinal ganglial areas in a bilateralway as appropriate (AU)


Subject(s)
Humans , Mediastinal Neoplasms/surgery , Sternotomy , Lung Neoplasms/surgery , Surgical Procedures, Operative/methods
17.
Nutr Hosp ; 27(4): 1204-12, 2012.
Article in Spanish | MEDLINE | ID: mdl-23165563

ABSTRACT

BACKGROUND: Consumption of green tea, by its antioxidant properties, has been associated with beneficial health effects, because antioxidant may play a role in the risk and pathogenesis of several chronic diseases, especially cardiovascular disease and cancer. On the other hand, it has been reported that metal compounds such as chromium [VI] are carcinogenic and can induce genotoxic damage through the Oxidative Stress. Therefore, it is possible that green tea has a protective effect against the genotoxic damage induced by this compounds. OBJECTIVE: To evaluate the effect of oral administration of green tea over the genotoxic damage induced by Cr [VI] by quantification of micronucleus (MN) in polychromatic erythrocytes (EPC). MATERIALS AND METHODS: We use mice of CD-1 strain that were randomly divided into the following groups: (i) control, (ii) treatment with green tea, (iii) treatment with chromium trioxide, (iv) treatment with green tea and chromium trioxide. The green tea was administrated via intragastric tube every 12 hours over two days (4 doses of 0.25 ml infusions 1.6 g/7.5 ml) and ad libitum (5.6 ml/day for 10 days infusions of 3.2 g/100 ml), while chromium trioxide was administrated via intraperitoneal (20 mg/kg). Blood samples were obtained from the caudal vein, the number of MN in EPC was assessed at 0, 24, 48 and 72 hours after the treatments. RESULTS: The group treated with green tea showed no significant statistical changes in the average of MN. On the other hand, the group that was dosed with the chromium trioxide showed an increase between 4 and 8 MN, which was statistically significant when compared with control group, which confirmed the genotoxic damage. When the green tea treatment was administered before the application of chromium trioxide, there was a decrease in MN frequencies of 31 and 62% at 72 hours, 20 and 35% at 48 hours and 18 and 31% at 24 hours with intragastric and ad libitum respectively, compared with the group treated only with chromium trioxide. Hence, green tea reduced the genotoxic damage induced by chromium trioxide, and the highest protection was presented at 72 hours. CONCLUSIONS: Our findings support the protective effects of green tea against the damage of genetic material, induced by metal compounds such as chromium [VI], suggesting that its antioxidant compounds are those that have a chemopreventive effect on the EOX generated by the Cr [VI] during its reduction to Cr (III). The fact that the largest decrease in the frequency of MN was observed at 72 hours and ad libitum treatment, suggests that, the protective effect depends on the bioavailability, pharmacodynamics and pharmacokinetics of the active ingredient in green tea, so the administration of green tea for a long period of time before the exposure to Cr [VI] could have a more consistent preventive effect.


Subject(s)
Antimutagenic Agents , Carcinogens/toxicity , Chromium Compounds/toxicity , Tea , Animals , Chemoprevention , Female , Male , Mice , Micronucleus Tests , Mutagenesis/drug effects
20.
Enferm. univ ; 8(1): 7-16, Ene.-mar. 2011. ilus
Article in Spanish | LILACS, BDENF - Nursing | ID: biblio-1028559

ABSTRACT

Objetivo: Conocer las tendencias y características de los artículos de investigación en enfermería publicados en México en los últimos 5 años. Metodología: Estudio descriptivo, retrospectivo y transversal. La muestra correspondió a 358 artículos de investigación publicados. Se elaboró un instrumento de 34 ítems, sometido al análisis de expertos y piloteado previo a la revisión final para valorar características de los artículos publicados en cuatro revistas mexicanas. Resultados: La principal temática abordada fue la clínica 36.9% en el contexto hospitalario, 42.2% de las investigaciones utilizó teoría de enfermería. En el 77.5% predominó el paradigma cuantitativo, la zona centro del país tiene el mayor número de publicaciones. En los artículos, el grado académico predominante del 1er autor es Maestría con el 41.1%.Discusión: Los resultados evidencian la brecha existente entre universidades y hospitales para generar investigación, falta de financiamiento (no hay claridad de su procedencia), insuficiente generación de nuevos investigadores (los alumnos no participan en la autoría), rigurosidad metodológica cuestionable, con evidentes inconsistencias en algunos artículos. Dado que no se analizaron revistas internacionales y revistas diferentes a la disciplina donde publican enfermeras mexicanas, los resultados no se pueden generalizar. A pesar de que los resultados son congruentes con estudios previos, es eminente la mejoría en la calidad de los artículos de investigación.Conclusión: El trabajo deja la oportunidad para fortalecer aspectos principalmente metodológicos que contribuyan al desarrollo de la investigación y propiciar la reflexión sobre las áreas de oportunidad en la investigación de enfermería mexicana.


Objective: Get to know the trends and features of published nursing research in Mexico in the last five years. Method. Descriptive, retrospective, transversal study. The sample was 358 published research articles. A 34 item instrument was elaborated, subdued to experts analysis, and piloted previously to the final review to asses features of the articles published in four Mexican journals. Outcomes: Clinical themes were the main subject approached: 36.9% in the hospital context; 42.2% researched used nursing theory. The qualitative paradigm prevailed in the 77.5%, and the country central area has the greater number of publications. In the articles, Mastery is the academic degree of the first author with 41.1%. Discussion: Results evidence the existing gap among universities and hospitals in order to generate research, financing lack (there is no clarity on its origin), inadequate generation of new researchers (students do not participate as authors), questionable methodological strictness, with evident inconsistencies in some articles. Due international or other than nursing journals where Mexican nurses published were not analyzed, results con not be generalized. Despite results are congruent with previous studies, the improvement on the research articles quality is evident (is eminent). Conclusion: The study leaves the opportunity open to strengthen mainly methodological aspects that contribute to research development and foster reflection on strengthen opportunity areas in Mexican nursing research.


Subject(s)
Humans , Male , Female , Nursing , Research
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