ABSTRACT
BACKGROUND: Cellular prion protein (PrP(C) ) is expressed in the enteric nervous system (ENS), however, its physiological role has not been identified. Studies suggest that PrP(C) can function as a metal-binding protein, as absence of the protein has been linked to altered copper metabolism and atypical synaptic activity. Because copper is known to modulate smooth muscle relaxation, we tested the hypothesis that PrP(C) deficiency would alter intestinal contractility. METHODS: We examined electrically evoked ileal contractility in Prnp(-/-) or wild type littermate mice and the effects of copper or copper chelation. PrP(C) expression was studied in whole mount ileal preparations of mice and guinea pigs by immunohistochemistry. KEY RESULTS: Relative to wild type mice, ileal tissues of Prnp(-/-) mice exhibited reduced electrical field stimulation (EFS)-evoked contractility. Furthermore, EFS-induced relaxation, as a percentage of that induced by a nitric oxide donor, was enhanced. Addition of a copper donor to the organ bath increased, whereas the addition of a copper chelator inhibited, nitric oxide donor-induced ileal relaxation in Prnp(-/-) mice. PrP(C) was expressed on nerve fibers or terminals, and some cell bodies in the myenteric and submucosal plexuses of wild type mice. PrP(C) colocalized with a neuron-specific ectonucleotidase, nucleoside triphosphate diphosphohydrolase 3 (NTPDase3), but to only a limited extent with GFAP, a marker of enteric glia. Guinea pigs expressed PrP(C) in nerve fibers or terminals and enteric glia in the myenteric and submucosal plexuses. CONCLUSIONS & INFERENCES: Our findings suggest that PrP(C) , which is abundant in the ENS, has a role in the regulation of ileal contractility.
Subject(s)
Copper/physiology , Ileum/physiology , Muscle Contraction/physiology , Muscle Relaxation/physiology , Muscle, Smooth/physiology , PrPC Proteins/physiology , Animals , Chelating Agents , Copper/metabolism , Enteric Nervous System/physiology , Guinea Pigs , Ileum/innervation , In Vitro Techniques , Male , Mice , Mice, Transgenic , Muscle, Smooth/innervation , Neuroglia/metabolism , Neurons/metabolism , PrPC Proteins/deficiency , PrPC Proteins/metabolismABSTRACT
Tamoxifen is capable of preserving bone mass in gonadectomized rodents as well as intact female mice; however, a detailed 3D quantitative analysis of the structural changes produced in the growing skeleton of intact mice of both genders by this agent is lacking. Employing quantitative microcomputed tomography (muCT), we assessed the effects of 4-hydroxytamoxifen (OHT) on the femora of C57BL/6J mice administered this agent either for 12 (males and females) or 2 (females) weeks. In mice of either gender, but especially in females, 12 weeks of OHT exposure led to dramatic increases in both cortical and trabecular bone. Females exposed to OHT for either 2 or 12 weeks demonstrated significantly increased cortical wall thickness, trabecular bone volume, connectivity, and number, as well as decreased trabecular separation. Significant increases in several of these parameters were also evident in males after 12 weeks of OHT administration. In view of the expanding use of OHT to induce Cre-mediated recombination events, our findings suggest that care should be exercised when interpreting the skeletal phenotypes of mice exposed this agent, particularly in situations where the effects of OHT might synergize with the phenotypic outcome of a specific genetic alteration.
Subject(s)
Bone and Bones/drug effects , Tamoxifen/pharmacology , Animals , Bone and Bones/anatomy & histology , Bone and Bones/ultrastructure , Drug Administration Schedule , Female , Femur/anatomy & histology , Femur/drug effects , Femur/ultrastructure , Male , Mice , Mice, Inbred C57BL , Organ Size/drug effects , Tamoxifen/administration & dosage , Time Factors , Tomography, X-Ray ComputedABSTRACT
Understanding the developmental and genetic basis for evolutionarily significant morphological variation in complex phenotypes such as the mammalian skull is a challenge because of the sheer complexity of the factors involved. We hypothesize that even in this complex system, the expression of phenotypic variation is structured by the interaction of a few key developmental processes. To test this hypothesis, we created a highly variable sample of crania using four mouse mutants and their wild-type controls from similar genetic backgrounds with developmental perturbations to particular cranial regions. Using geometric morphometric methods we compared patterns of size, shape, and integration in the sample within and between the basicranium, neurocranium, and face. The results highlight regular and predictable patterns of covariation among regions of the skull that presumably reflect the epigenetic influences of the genetic perturbations in the sample. Covariation between relative widths of adjoining regions is the most dominant factor, but there are other significant axes of covariation such as the relationship between neurocranial size and basicranial flexion. Although there are other sources of variation related to developmental perturbations not analyzed in this study, the patterns of covariation created by the epigenetic interactions evident in this sample may underlie larger scale evolutionary patterns in mammalian craniofacial form.
Subject(s)
Epigenesis, Genetic , Skull/anatomy & histology , Animals , Base Sequence , DNA Primers , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , PhenotypeABSTRACT
Tight regulation of the phosphatidylinositiol 3-kinase (PI3K) pathway is essential not only for normal immune system development and responsiveness, but also in the prevention of immunopathology. Indeed, unchecked activation of the PI3K pathway in T cells induces lymphoproliferation and systemic autoimmunity. Evaluating the importance of threshold levels of two key PI3K pathway phosphoinositol phosphatases, we previously reported that mice heterozygous for both Pten and SHIP develop a more rapid progression of a lymphoproliferative autoimmune syndrome than do Pten(+\-) mice. Investigating the basis for this difference, we now describe a quantitative and qualitative difference in the antibody responses of C57BL\6 Pten(+\-) SHIP(+\-) mice upon challenge with a T-dependent antigen. Suspecting that this phenotypic difference might be the result, at least in part, of a T-helper cell defect, an in vitro analysis of anti-CD3/interleukin (IL)-2-expanded CD4(+) T cells was performed. After stimulation with anti-CD3, cells from mice heterozygous for both Pten and SHIP exhibited a striking increase in IL-4 secretion (> 10-fold), without a corresponding increase in T helper 2 (Th2) cell numbers being evident by intracellular staining for this cytokine. Modest increases were also seen for both IL-13 and IFN-gamma. Perhaps in keeping with this abnormal in vitro cytokine profile, IgG1 serum levels were significantly elevated in young C57BL\6 Pten(+\-) SHIP(+\-) mice. Thus, the relative levels of Pten and SHIP appear to be key variables in CD4(+) T-cell function, primarily via their ability to regulate IL-4 production.
Subject(s)
CD3 Complex/immunology , CD4-Positive T-Lymphocytes/immunology , Cytokines/immunology , Phosphoric Monoester Hydrolases/genetics , Protein Tyrosine Phosphatases/genetics , Tumor Suppressor Proteins/genetics , Animals , Antibody Formation , Apoptosis , Enzyme-Linked Immunosorbent Assay/methods , Female , Heterozygote , Humans , Immunoblotting/methods , Immunoglobulin G/blood , Interferon-gamma/immunology , Interleukin-13/immunology , Interleukin-4/immunology , Mice , Mice, Inbred C57BL , Mice, Transgenic , PTEN Phosphohydrolase , Phosphatidylinositol 3-Kinases/metabolism , Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases , Signal TransductionABSTRACT
Phosphatidylinositol 3-kinase (PI3K) has emerged as a critical component of multiple immune system intracellular signalling pathways. The levels and relative ratios of PI3K products, phosphatidylinositol (3,4) bisphosphate (PI(3,4)P(2)) and phosphatidylinositol (3,4,5) trisphosphate (PIP(3)), are regulated by inositol phosphatases such as Pten and SHIP. Interestingly, mice heterozygous for Pten, a 3'-inositol phosphatase, develop a progressive lymphoproliferative syndrome with autoimmune features. Given the importance of PIP(3) species in regulating immune responses, we hypothesized that heterozygosity for the 5'-inositol phosphatase SHIP might exacerbate the autoimmune phenotype of Pten(+/-) mice. In keeping with this, mice heterozygous for both Pten and SHIP developed lymphoproliferation, hypergammaglobulinaemia, autoantibody titres and renal pathology that were more severe than that of Pten(+/-) mice. These results suggest that the relative levels of phosphatidylinositol phosphatases are likely critical to immune system homeostasis and they also highlight the potential for gene dosage effects in regulating susceptibility and/or severity of autoimmunity.
Subject(s)
Genetic Carrier Screening , Loss of Heterozygosity/genetics , Lymphatic Diseases/genetics , Lymphatic Diseases/pathology , Phosphoric Monoester Hydrolases/genetics , Tumor Suppressor Proteins/genetics , Animals , Cells, Cultured , Female , Kidney/immunology , Kidney/pathology , Loss of Heterozygosity/immunology , Lymphatic Diseases/immunology , Mice , Mice, Mutant Strains , PTEN Phosphohydrolase , Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases , Phosphoric Monoester Hydrolases/deficiency , Phosphoric Monoester Hydrolases/immunology , Spleen/immunology , Spleen/pathology , Tumor Suppressor Proteins/deficiency , Tumor Suppressor Proteins/immunologyABSTRACT
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a hormone belonging to the glucagon superfamily of hormones. These hormones are known to play important roles in metabolism and growth. PACAP is a neuropeptide that causes accumulation of cAMP in a number of tissues and affects the secretion of other hormones, vasodilation, neural and immune functions, as well as the cell cycle. To determine whether PACAP is essential for survival and to evaluate its function(s), we have generated mice lacking the PACAP gene via homologous recombination. We found that most PACAP null mice died in the second postnatal week in a wasted state with microvesicular fat accumulation in liver, skeletal muscle, and heart. Gas chromatography-mass spectrometry showed that fatty acid beta-oxidation in liver mitochondria of PACAP(-/-) mice was not blocked based on the distribution of 3-hydroxy-fatty acids (C6-16) in the plasma. Instead, increased metabolic flux through the beta-oxidation pathway was suggested by the presence of ketosis. Also, serum triglycerides and cholesterol were significantly higher (2- to 3-fold) in PACAP null mice than littermates. In the fed state, both serum insulin and blood glucose were normal in 5-d-old null mice compared with their littermates. In contrast, fasted PACAP null pups had a significant increase in insulin, but a decrease in blood glucose compared with littermates. Glycogen in the liver was reduced. These results suggest PACAP is a critical hormonal regulator of lipid and carbohydrate metabolism.
Subject(s)
Carbohydrate Metabolism , Lipid Metabolism , Neuropeptides/deficiency , Neuropeptides/genetics , Adipose Tissue/metabolism , Adrenal Glands/metabolism , Animals , Blood Glucose/metabolism , Blotting, Western , Brain Chemistry , Cholesterol/blood , Fatty Acids/metabolism , Fatty Acids, Nonesterified/blood , Gene Targeting , Glycogen/metabolism , Insulin/blood , Ketone Bodies/blood , Ketosis/genetics , Liver/chemistry , Mice , Mice, Knockout , Mitochondria, Liver/metabolism , Mortality , Muscle, Skeletal/metabolism , Myocardium/metabolism , Neuropeptides/physiology , Oxidation-Reduction , Pituitary Adenylate Cyclase-Activating Polypeptide , RNA, Messenger/analysis , Triglycerides/blood , Wasting Syndrome/genetics , Wasting Syndrome/mortalityABSTRACT
Mutations of PTEN, which encodes a protein-tyrosine and lipid phosphatase, are prevalent in a variety of human cancers. The human genome 'draft' sequence still lacks organization and much of the PTEN and adjacent loci remain undefined. The pufferfish, Fugu rubripes, by virtue of having a compact genome represents an excellent template for rapid vertebrate gene discovery. Sequencing of 56 kb from the Fugu pten (fpten) locus identified four complete genes and one partial gene homologous to human genes. Genes neighboring fpten include a PAPS synthase (fpapss2) differentially expressed between non-metastatic/metastatic human carcinoma cell lines, an inositol phosphatase (fminpp1) and an omega class glutathione-S-transferase (fgsto). We have determined the order of human BAC clones at the hPTEN locus and that the locus contains hPAPSS2 and hMINPP1 genes oriented as are their Fugu orthologs. Although the human genes span 500 kb, the Fugu genes lie within only 22 kb due to the compressed intronic and intergenic regions that typify this genome. Interestingly, and providing striking evidence of regulatory element conservation between widely divergent vertebrate species, the compact 2.1 kb fpten promoter is active in human cells. Also, like hPTEN, fpten has a growth and tumor suppressor activity in human glioblastoma cells, demonstrating conservation of protein function.
Subject(s)
Evolution, Molecular , Fishes/genetics , Genetic Linkage , Phosphoric Monoester Hydrolases/genetics , Protein Tyrosine Phosphatases/genetics , Protein Tyrosine Phosphatases/physiology , Tumor Suppressor Proteins , Amino Acid Sequence , Animals , Cell Division , Cloning, Molecular , Conserved Sequence , Fish Proteins , Fishes/metabolism , Genes, Tumor Suppressor , Glioblastoma/pathology , Glutathione Transferase/genetics , Humans , Molecular Sequence Data , Multienzyme Complexes/genetics , PTEN Phosphohydrolase , Phosphoric Monoester Hydrolases/physiology , Promoter Regions, Genetic , RNA, Messenger/biosynthesis , Sequence Homology, Amino Acid , Sulfate Adenylyltransferase/genetics , Tumor Cells, CulturedABSTRACT
The ability of specific virally encoded proteins to down-regulate MHC class I molecules may enable infected cells to elude killing by CTL. In the case of HIV-1, Nef appears to be responsible for this effect. Thus, interfering with Nef-induced MHC class I down-regulation would be a strategy for increasing HIV-1-specific CTL activity, particularly towards long-lived T cell populations such as memory T cells that harbor replication-competent virus. Here, using two Nef-expressing human cell model systems, we show that a dominant-negative mutant derived from the Hck protein-tyrosine kinase, composed of the Hck N-terminal region, as well as the SH3 and SH2 domains, was able to inhibit Nef-induced MHC class I molecule down-regulation. This effect was SH3 domain dependent as it was not evident when the cells were transfected with DN-Hck-W93F, an SH3 domain mutant. The inhibitory effect of dominant-negative-Hck (DN-Hck) on Nef-induced class I down-regulation suggests that this Nef-mediated effect requires an interaction between the Nef polyproline site and an SH3-containing cellular protein that is involved in MHC class I molecule turnover. Interfering with the function of the Nef SH3 binding site in this way represents a strategy for assisting the host CTL response to clear HIV-1-infected cells.
Subject(s)
Down-Regulation , Gene Products, nef/antagonists & inhibitors , Gene Products, nef/metabolism , HIV-1/immunology , Histocompatibility Antigens Class I/metabolism , Protein-Tyrosine Kinases/metabolism , Proto-Oncogene Proteins/metabolism , src Homology Domains/physiology , Binding Sites , Blotting, Western , CD4 Antigens/metabolism , Cell Line , Flow Cytometry , Gene Products, nef/chemistry , Gene Products, nef/genetics , Genes, Dominant , HIV-1/genetics , Histocompatibility Antigens Class I/immunology , Humans , Mutation/genetics , Peptides/genetics , Peptides/metabolism , Precipitin Tests , Protein Binding , Protein-Tyrosine Kinases/chemistry , Protein-Tyrosine Kinases/genetics , Proto-Oncogene Proteins/chemistry , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins c-hck , T-Lymphocytes, Cytotoxic/immunology , Transfection , nef Gene Products, Human Immunodeficiency Virus , src Homology Domains/geneticsABSTRACT
Several lines of evidence point to an abnormality in the response of Fanconi anemia cells to reactive oxygen species. To investigate the potential pathologic consequences of an in vivo alteration of redox state in mice lacking one of the Fanconi anemia genes, animals were generated having combined deficiencies of the cytosolic Cu/Zn superoxide dismutase (Sod1) and Fanconi anemia complementation group C (Fancc) genes. Interestingly, hepatocytes of Fancc(-/-)Sod1(-/-) mice exhibited a zonal pattern of microvesicular steatosis, possibly as a result of oxidative stress-induced injury to hepatocyte membranes. Consistent with this idea, freshly explanted Fancc(-/-)Sod1(-/-) hepatocytes demonstrated increased spontaneous production of superoxide in vitro. The second phenotypic feature of Fancc(-/-) Sod1(-/-) mice was that of bone marrow hypocellularity accompanied by significant decreases in peripheral blood erythrocyte and leukocyte numbers as compared with wild-type controls. Although flow cytometry analysis with monoclonal antibodies against cell surface antigens revealed normal numbers of primitive hematopoietic progenitor populations in Fancc(-/-)Sod1(-/-) marrow, lineage-positive progenitor numbers were significantly reduced in these mice. Furthermore, the in vitro clonogenic growth of Fancc(-/-)Sod1(-/-) erythroid, myeloid, and early B-lymphoid colonies in semisolid media was profoundly compromised. These results suggested that the altered redox state likely present in Fancc(-/-) Sod1(-/-) hematopoietic progenitors was responsible for an impairment of cell proliferation or survival. (Blood. 2001;98:1003-1011)
Subject(s)
Cell Cycle Proteins , DNA-Binding Proteins , Fatty Liver/genetics , Hematopoiesis/genetics , Nuclear Proteins , Proteins/genetics , Superoxide Dismutase/genetics , Animals , Bone Marrow Cells/pathology , Bone Marrow Diseases/pathology , Disease Models, Animal , Erythrocyte Count , Erythrocytes/pathology , Fanconi Anemia/complications , Fanconi Anemia/pathology , Fanconi Anemia Complementation Group C Protein , Fanconi Anemia Complementation Group Proteins , Fatty Liver/complications , Leukopenia/etiology , Leukopenia/genetics , Mice , Mice, Knockout , Oxidation-Reduction , Proteins/pharmacology , Superoxide Dismutase/deficiency , Superoxide Dismutase/pharmacology , Superoxides/metabolismABSTRACT
Mutations in DNA mismatch repair (MMR) genes are associated with increased genomic instability and susceptibility to cancer. Mice rendered deficient in either Mlh1 or Pms2 as a result of gene targeting are prone to tumorigenesis, particularly, lymphomas. In addition, although Mlh1-/- mice also develop small intestinal adenomas and adenocarcinomas, Pms2-/- animals remain free of such tumors. To establish whether this phenotypic dichotomy might be associated with a quantitative and/or qualitative difference in genomic instability in these mice, we determined small intestinal epithelial cell DNA mutant frequency and mutation spectrum using a transgenic lambda-phage lacI reporter system. Mutant frequencies obtained from both Mlh1-/- and Pms2-/- mice revealed elevations of 18- and 13-fold, respectively, as compared to their wild-type littermates. Interestingly, we found that C : G-->T : A transitions were significantly elevated in Mlh1-/- mice, accounting in large measure for the 1.5-fold lacI mutant frequency increase seen in these animals. We hypothesize that the increased level of C : G-->T : A mutations may explain, in part, why Mlh1-/- mice, but not Pms2-/- mice, develop small intestinal tumors. Furthermore, the difference in the lacI mutational spectrum of Mlh1-/- and Pms2-/- mice suggests that other MutL-like heterodimers may play important roles in the repair of G : T mispairs arising within murine small intestinal epithelial cells.
Subject(s)
Adenosine Triphosphatases , DNA Repair Enzymes , DNA-Binding Proteins , Intestine, Small/physiology , Neoplasm Proteins/genetics , Point Mutation , Adaptor Proteins, Signal Transducing , Animals , Base Pair Mismatch/genetics , Carrier Proteins , Crosses, Genetic , DNA Mutational Analysis , Epithelial Cells/physiology , Female , Intestinal Neoplasms/genetics , Intestine, Small/cytology , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Transgenic , Mismatch Repair Endonuclease PMS2 , MutL Protein Homolog 1 , Nuclear ProteinsABSTRACT
We reported previously that thymic lymphomas arising in mice lacking the DNA mismatch repair (MMR) gene, Msh2(-/-), exhibited striking elevations in the mutation frequency of a transgenic lacI reporter gene when compared with normal Msh2(-/-) tissues. To investigate whether hypermutation was a feature of all tumors arising in MMR-deficient mice, lacI transgene mutation frequencies were obtained from several different mouse tumors deficient for PMS2 and/or MSH2. While lacI gene hypermutation was again clearly evident in Msh2 +/- ms2(-/-) and Msh2(-/-)Pms2(-/-) thymic lymphomas, three non-thymic MSH2-deficient tumors failed to show lacI gene mutation frequency elevations when compared with a normal tissue of MMR-deficient mice. The elevated mutation frequencies in the lymphoid tumors, and the finding of multiple clustered mutations in lacI genes rescued from these tumors, suggest that they are possibly generated by a lymphoma-specific hypermutational mechanism.
Subject(s)
Base Pair Mismatch , DNA Repair , DNA-Binding Proteins , Escherichia coli Proteins , Genes, Reporter , Animals , Bacterial Proteins/genetics , Lac Repressors , Lymphoma/genetics , Mice , Mice, Transgenic , MutS Homolog 2 Protein , Mutation , Proto-Oncogene Proteins/genetics , Repressor Proteins/genetics , Thymus Neoplasms/geneticsABSTRACT
We have generated transgenic mice by pronuclear microinjection of a murine satellite DNA-based artificial chromosome (SATAC). As 50% of the founder progeny were SATAC-positive, this demonstrates that SATAC transmission through the germline had occurred. FISH analyses of metaphase chromosomes from mitogen-activated peripheral blood lymphocytes from both the founder and progeny revealed that the SATAC was maintained as a discrete chromosome and that it had not integrated into an endogenous chromosome. To our knowledge, this is the first report of the germline transmission of a genetically engineered mammalian artificial chromosome within transgenic animals generated through pronuclear microinjection. We have also shown that murine SATACs can be similarly introduced into bovine embryos. The use of embryo microinjection to generate transgenic mammals carrying genetically engineered chromosomes provides a novel method by which the unique advantages of chromosome-based gene delivery systems can be exploited.
Subject(s)
Cell Nucleus/genetics , Chromosomes/genetics , DNA, Satellite/genetics , Gene Transfer Techniques , Oocytes/cytology , Animals , Cattle , Embryo, Mammalian , Female , Flow Cytometry , In Situ Hybridization, Fluorescence , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Transgenic , Microinjections , Polymerase Chain ReactionABSTRACT
DNA mismatch repair (MMR) deficiency leads to an increased mutation frequency and a predisposition to neoplasia. 'Knockout' mice deficient in the MMR proteins Msh2 and Pms2 crossed with mutation detection reporter (supF, lacI and cII) transgenic mice have been used to facilitate a comparison of the changes in mutation frequency and spectra. We find that the mutation frequency was consistently higher in Msh2-deficient mice than Pms2-deficient mice. The lacI target gene, which is highly sensitive to point mutations, demonstrated that both Msh2- and Pms2-deficient mice accumulate transition mutations as the predominant mutation. However, when compared with Msh2(-/-) mice, lacI and cII mutants from Pms2-deficient mice revealed an increased proportion of +/-1 bp frameshift mutations and a corresponding decrease in transversion mutations. The supF target gene, which is sensitive to frameshift mutations, and the cII target gene revealed a strong tendency for -1 bp deletions over +1 bp insertions in Msh2(-/-) compared with Pms2(-/-) mice. These data indicate that Msh2 and Pms2 deficiency have subtle but differing effects on mutation avoidance which may contribute to the differences in tumor spectra observed in the two 'knockout' mouse models. These variances in mutation accumulation may also play a role, in part, in the differences seen in prevalence of MSH2 and PMS2 germline mutations in hereditary non-polyposis colorectal cancer patients.
Subject(s)
Adenosine Triphosphatases , DNA Repair Enzymes , DNA-Binding Proteins , Mutagenesis/genetics , Proteins/metabolism , Proto-Oncogene Proteins/metabolism , Animals , Base Pair Mismatch , Crosses, Genetic , DNA Repair , Female , Frameshift Mutation , Genes, Reporter , Genotype , Germ-Line Mutation , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Mismatch Repair Endonuclease PMS2 , MutS Homolog 2 Protein , Point Mutation , Proteins/genetics , Proto-Oncogene Proteins/geneticsABSTRACT
SHPS-1 (SH2-domain bearing protein tyrosine phosphatase (SHP) substrate-1), a member of the inhibitory-receptor superfamily that is abundantly expressed in macrophages and neural tissue, appears to regulate intracellular signaling events downstream of receptor protein-tyrosine kinases and integrin-extracellular matrix molecule interactions. To investigate the function of SHPS-1 in a hematopoietic cell line, SHPS-1 was expressed in Ba/F3 cells, an IL-3-dependent pro-B-cell line that lacks endogenous SHPS-1 protein. Interestingly, expression of either SHPS-1, or a mutant lacking the intracellular domain of SHPS-1 (DeltaCT SHPS-1), resulted in the rapid formation of macroscopic Ba/F3 cell aggregates. As the integrin-associated protein/CD47 was shown to be a SHPS-1 ligand in neural cells, we investigated whether CD47 played a role in the aggregation of SHPS-1-expressing Ba/F3 cells. In support of this idea, aggregate formation was inhibited by an anti-CD47 Ab. Furthermore, erythrocytes from control, but not from CD47-deficient mice, were able to form rosettes on SHPS-1-expressing Ba/F3 cells. Because erythrocytes do not express integrins, this result suggested that SHPS-1-CD47 interactions can take place in the absence of a CD47-integrin association. We also present evidence that the amino-terminal Ig domain of SHPS-1 mediates the interaction with CD47. Although SHPS-1-CD47 binding likely triggers bidirectional intracellular signaling processes, these results demonstrate that this interaction can also mediate cell-cell adhesion.
Subject(s)
Antigens, CD/metabolism , Antigens, Differentiation , B-Lymphocytes/immunology , Carrier Proteins/metabolism , Cell Communication/immunology , Membrane Glycoproteins/physiology , Neural Cell Adhesion Molecule L1 , Neural Cell Adhesion Molecules/physiology , Receptors, Immunologic , Stem Cells/immunology , Animals , Antibodies, Blocking/pharmacology , Antigens, CD/genetics , Antigens, CD/immunology , Antigens, CD/physiology , B-Lymphocytes/metabolism , CD47 Antigen , Carrier Proteins/genetics , Carrier Proteins/immunology , Carrier Proteins/physiology , Cell Aggregation/genetics , Cell Aggregation/immunology , Cell Communication/genetics , Cell Line , Extracellular Space/immunology , Extracellular Space/metabolism , Membrane Glycoproteins/biosynthesis , Membrane Glycoproteins/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Neural Cell Adhesion Molecules/biosynthesis , Neural Cell Adhesion Molecules/metabolism , Peptide Fragments/physiology , Protein Structure, Tertiary , Rats , Rosette FormationABSTRACT
DNA mismatch repair (MMR) proteins recognize nucleotides that are incorrectly paired. Deficiencies in MMR lead to increased genomic instability reflected in an increased mutation frequency and predisposition to tumorigenesis. Mice lacking the MMR gene, Msh2, develop thymic lymphomas that exhibit much higher mutational frequencies than other Msh2(-/-) tumours and Msh2(-/-) normal thymic tissue, suggesting that an additional mutator may have been acquired in a tissue-specific manner. Clustered mutations observed exclusively in the thymic lymphomas suggests that a gene(s) associated with the replication machinery might have become altered during tumorigenesis. Based on mutation studies in Saccharomyces cerevisiae lacking Msh2 and DNA polymerase delta (DNA pol delta), we hypothesized that the acquisition of mutations in DNA pol delta could contribute to the hypermutator phenotype and tumorigenesis in Msh2(-/-) thymic tissue. Furthermore, previous reports have suggested that genes containing mononucleotide repeats are non-random mutational targets in the absence of MMR. Therefore, we sequenced all 26 exons of the DNA pol delta catalytic subunit, including the six exons containing mononucleotide repeats of >5 bp, from nine Msh2(-/-) thymic lymphomas and two wild-type controls. No DNA pol delta pathogenic mutations were found in the thymic lymphomas, although several DNA base differences compared with published DNA pol delta sequences were observed. We conclude, therefore, that inactivating mutations in DNA pol delta are not a contributing factor in the development of the hypermutator phenotype in MMR-deficient murine thymic lymphomas.
Subject(s)
Base Pair Mismatch/genetics , DNA Polymerase III/genetics , DNA Repair/genetics , DNA-Binding Proteins , Lymphoma/genetics , Proto-Oncogene Proteins/genetics , Thymus Neoplasms/genetics , Animals , Base Sequence , DNA Primers , Exons , Introns , Mice , Mice, Knockout , Molecular Sequence Data , MutS Homolog 2 Protein , Mutation , Proto-Oncogene Proteins/deficiencyABSTRACT
Mice heterozygous for the tight-skin (Tsk) mutation develop skin fibrosis. Previous studies have implicated a role for the immune system and, specifically, CD4(+) T cells, in the etiology of skin fibrosis in Tsk/+ mice. We have recently shown that the administration of neutralizing anti-IL-4 antibodies to Tsk/+ mice prevented the development of skin fibrosis in these mice. Since IL-4 is a major cytokine produced by T helper 2 (Th2) cells, we investigated the role of Th2 cells in mediating skin fibrosis in Tsk/+ mice. Previous studies have shown that the development of Th2 cells in non-Tsk mice is abrogated in mice with null mutation for either the IL-4 or the Stat6 gene. In this study we showed that the polarization of CD4(+) T cells from Tsk/+ mice toward the Th2 lineage is also dependent on a functioning IL-4 or Stat6 gene. More importantly, the development of skin fibrosis in Tsk/+ mice was abrogated by the IL4(-/-) or the Stat6(-/-) mutation. We also determined whether alteration of the TCR repertoire in Tsk/+ mice, achieved by the introduction of TCR transgenes, was able to prevent the development of skin fibrosis in Tsk/+ mice. We found that the exclusive usage of the Vbeta8.2 gene segment by T cells was sufficient to prevent skin fibrosis in Tsk/+ mice. This result suggests that the exclusive use of this Vbeta gene segment by T cells may have prevented the development of fibrosis-causing Th2 cells.
Subject(s)
Scleroderma, Systemic/immunology , Th2 Cells/immunology , Animals , Cells, Cultured , Disease Models, Animal , Female , Fibrosis/immunology , Fibrosis/pathology , H-2 Antigens/immunology , Interferon-gamma/biosynthesis , Interleukin-4/biosynthesis , Interleukin-4/genetics , Interleukin-4/immunology , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Mice, Mutant Strains , Mice, Transgenic , Peptide Fragments/genetics , Peptide Fragments/immunology , Receptors, Antigen, T-Cell, alpha-beta/genetics , Receptors, Antigen, T-Cell, alpha-beta/immunology , STAT6 Transcription Factor , Scleroderma, Systemic/pathology , Th1 Cells/immunology , Th1 Cells/metabolism , Trans-Activators/genetics , Trans-Activators/immunologyABSTRACT
We have compared the spontaneous mutation frequency and spectrum of lacI genes recovered from a rat embryonic fibroblast line transfected with a lambda-phage shuttle vector (Rat2lambdalacI) using both the traditional plaque assay as well as a positive selection assay. In addition, mutation frequencies and spectrum were determined after treatment of the cells with either the intracellular superoxide-generating compound, menadione, or UVC light. The differences in mutation frequency between the two systems suggested that the selectable assay was better at discerning relatively small mutation frequency increases, more rapidly and at lower cost, than the plaque assay method. Some novel lacI mutations were observed in mutants derived from the selectable assay. This indicates that the selectable assay system may be a useful tool for assessing the mutagenic potential of different agents.
Subject(s)
Bacterial Proteins/genetics , Escherichia coli Proteins , Mutagens/toxicity , Repressor Proteins/genetics , Vitamin K/toxicity , Animals , Bacteriophage lambda/genetics , DNA Damage/genetics , Fibroblasts , Lac Repressors , Mutagenesis , Mutation/genetics , Rats , Superoxides/metabolism , Transfection , Ultraviolet Rays , Viral Plaque AssayABSTRACT
Stromal cell-derived factor-1 (SDF-1), a C-X-C family chemokine, is a potent T lymphocyte chemoattractant. We investigated the effects of T cell activation on the chemotactic response to SDF-1. Anti-CD3 Ab stimulation of either Jurkat T cells or murine peripheral CD4+ T lymphocytes produced a dramatic inhibition of SDF-1-induced chemotaxis. In contrast, the SDF-1 responses of Jurkat clones with deficiencies in key TCR signaling components (Lck, CD45, and TCR-beta), were only marginally reduced by anti-CD3 stimulation. Similar to PMA treatment, which abolished both CXCR4 receptor expression and the chemotactic response of Jurkat cells to SDF-1, anti-CD3 Ab treatment reduced cell surface expression of CXCR4 to 65% of the control value, an effect that was blocked by protein kinase C inhibitors. Our data suggest that initial T cell activation events inhibit the response of Jurkat T cells to CXCR4 stimulation. In contrast, SDF-1 treatment resulted in a reduction of tyrosine phosphorylation of the TCR downstream effectors, ZAP-70, SLP-76, and LAT (linker for activation of T cells), suggesting that this chemokine potentially regulates the threshold for T cell activation.
Subject(s)
Adaptor Proteins, Signal Transducing , Chemokines, CXC/antagonists & inhibitors , Chemokines, CXC/physiology , Chemotaxis, Leukocyte/immunology , Membrane Proteins , Receptors, Antigen, T-Cell/metabolism , Receptors, CXCR4/physiology , Animals , Antibodies, Blocking/pharmacology , Antibodies, Monoclonal/pharmacology , CD3 Complex/immunology , Carrier Proteins/metabolism , Cell Membrane/immunology , Cell Membrane/metabolism , Chemokine CXCL12 , Chemotaxis, Leukocyte/drug effects , Down-Regulation/drug effects , Down-Regulation/immunology , Humans , Jurkat Cells/immunology , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Molecular Weight , Muromonab-CD3/pharmacology , Phosphoproteins/antagonists & inhibitors , Phosphoproteins/metabolism , Phosphorylation , Protein Kinase C/physiology , Protein-Tyrosine Kinases/antagonists & inhibitors , Protein-Tyrosine Kinases/metabolism , Receptors, Antigen, T-Cell/physiology , Receptors, CXCR4/antagonists & inhibitors , Receptors, CXCR4/biosynthesis , Signal Transduction/immunology , Stromal Cells/physiology , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , Tetradecanoylphorbol Acetate/pharmacology , ZAP-70 Protein-Tyrosine KinaseABSTRACT
The roles of protein-tyrosine phosphatases (PTPs) in processes such as cell growth and adhesion are poorly understood. To explore the ability of specific PTPs to regulate cell signaling pathways initiated by stimulation of growth factor receptors, we expressed the receptor-like PTP, PTPalpha, in A431 epidermoid carcinoma cells. These cells express high levels of the epidermal growth factor (EGF) receptor and proliferate in response to the autocrine production of transforming growth factor-alpha. Conversely, EGF stimulation of A431 cells in vitro leads to growth inhibition and triggers the rapid detachment of these cells from the substratum. Although PTPalpha expression did not alter the growth characteristics of either unstimulated or EGF-stimulated cells, this phosphatase was associated with increased cell-substratum adhesion. Furthermore, PTPalpha-expressing A431 cells were strikingly resistant to EGF-induced cell rounding. Overexpression of PTPalpha in A431 cells was associated with the dephosphorylation/activation of specific Src family kinases, suggesting a potential mechanism for the observed alteration in A431 cell-substratum adhesion. Src kinase activation was dependent on the D1 catalytic subunit of PTPalpha, and there was evidence of association between PTPalpha and Src kinase(s). PTPalpha expression also led to increased association of Src kinase with the integrin-associated focal adhesion kinase, pp125(FAK). In addition, paxillin, a Src and/or pp125(FAK) substrate, displayed increased levels of tyrosine phosphorylation in PTPalpha-expressing cells and was associated with elevated amounts of Csk. In view of these alterations in focal adhesion-associated molecules in PTPalpha-expressing A431 cells, as well as the changes in adhesion demonstrated by these cells, we propose that PTPalpha may have a role in regulating cell-substratum adhesion.
Subject(s)
Cell Adhesion/physiology , Protein Tyrosine Phosphatases/metabolism , Protein-Tyrosine Kinases/metabolism , src Homology Domains , Amino Acid Sequence , Carcinoma, Squamous Cell , Cell Adhesion/drug effects , Cell Adhesion Molecules/metabolism , Cell Division/drug effects , Cell Size/drug effects , Cell Size/physiology , Cloning, Organism , Cytoskeletal Proteins/metabolism , Epidermal Growth Factor/pharmacology , Epidermal Growth Factor/physiology , ErbB Receptors/genetics , ErbB Receptors/metabolism , Focal Adhesion Kinase 1 , Focal Adhesion Protein-Tyrosine Kinases , Humans , Kinetics , Molecular Sequence Data , Paxillin , Peptide Fragments/chemistry , Phosphopeptides/chemistry , Phosphoproteins/metabolism , Protein Tyrosine Phosphatases/biosynthesis , Receptor, Insulin/metabolism , Recombinant Proteins/metabolism , Rosaniline Dyes/metabolism , Substrate Specificity , Transfection , Transforming Growth Factor alpha/pharmacology , Transforming Growth Factor alpha/physiology , Tumor Cells, CulturedABSTRACT
The tight-skin (Tsk/+) mutant mouse, a putative murine model of scleroderma, is characterized primarily by the excessive deposition of collagen and other extracellular matrix molecules in the dermis, and also by a developmentally acquired defect in pulmonary architecture. Passive transfer experiments have suggested an etiologic role for the immune system in Tsk/+ dermal pathology. In addition, CD4+ T lymphocytes have been shown to be required for the excessive accumulation of dermal collagen in these mice. As IL-4, a product of differentiated CD4+ T cells, is capable of regulating the synthesis of various matrix molecules (including type I collagen) by fibroblasts in vitro, we investigated the potential role of IL-4 in mediating Tsk/+ dermal fibrosis. Confirming that Tsk/+ cells are capable of responding to IL-4, we found receptors for this cytokine on Tsk/+ embryonic fibroblasts and a dermal fibroblast cell line derived from these mice. Furthermore, IL-4 receptors on Tsk/+ fibroblasts were functional since IL-4 stimulation in vitro increased type I collagen secretion from these cells. These results demonstrated the potential for IL-4 to be directly involved in the excessive deposition of dermal collagen in Tsk/+ mice. Critical insight into the role played by IL-4 in mediating the dermal phenotype, however, was obtained following the administration of neutralizing anti-lL-4 antibodies to Tsk/+ mice. This treatment prevented the development of dermal fibrosis, leading to normalization of dermal collagen content. Given the requirement for CD4+ T cells in Tsk/+ dermal fibrosis, our results suggest that Th2 cells and/or factors elaborated by this T cell subset may play a key role in regulating dermal collagen content in this strain.