ABSTRACT
Objective:To systematic review the efficacy and safety of external application of traditional Chinese medicine on treatment of breast cancer related lymphedema.Methods:CNKI, Wanfang Data, VIP, Sino-Med, The Cochrane Library, PubMed, Web of Science were searched for related randomized controlled trials, the retrieval time was from inception to May 25, 2020. Two researchers independently screened and evaluated the included studies. Meta-analysis was performed by RevMan 5.4 software.Results:A total of 16 studies involving 1 315 patients with breast cancer related lymphedema were included, and the methodological quality of the included studies was not high. Compared with conventional treatment, external application of traditional Chinese medicine combined with conventional treatment had advantages in improving the total efficiency( P<0.01) and quality of life( P<0.01), reducing pain( P<0.01) and improving upper limb function( P<0.01), without obvious adverse reactions( P>0.05), but there was no improvement in depression( P>0.05). Compared with conventional treatment, external application of traditional Chinese medicine could improve the total efficiency( P<0.01). Compared with placebo sticker combined with conventional treatment, external application of traditional Chinese medicine combined with conventional treatment can reduce circumference( P<0.05) and reduce pain( P<0.01), without obvious adverse reactions( P>0.05). Conclusions:Available evidence suggests that external application of traditional Chinese medicine may be a potential treatment method for breast cancer related lymphedema. Due to the poor methodological quality of the included studies, high-quality randomized controlled trials are still needed.
ABSTRACT
Ferroptosis is a recently recognized form of regulated cell death caused by an iron-dependent accumulation of lipid reactive species. However, little research on ferroptosis and lung cancer, one of the most common tumors, has been carried out. This paper tries to review the research progress of ferroptotic suppression and explain it from the different ways of ferroptosis occurrence. Furthermore, as inducing ferroptosis to treat cancer gets more attention, we introduce four kinds of ferroptosis-inducing compounds and new prospects for lung cancer therapy to provide new ideas for lung cancer treatment.
ABSTRACT
Ferroptosis is a recently recognized form of regulated cell death caused by an iron-dependent accumulation of lipid reactive species. However, little research on ferroptosis and lung cancer, one of the most common tumors, has been carried out. This paper tries to review the research progress of ferroptotic suppression and explain it from the different ways of ferroptosis occurrence. Furthermore, as inducing ferroptosis to treat cancer gets more attention, we introduce four kinds of ferroptosis-inducing compounds and new prospects for lung cancer therapy to provide new ideas for lung cancer treatment.
ABSTRACT
This paper reviews the literature about the common complications, evaluation tools and intervention measures of free flap repair for tongue cancer patients at home and abroad, summarizes the current research status, and points out that the nursing plan of free flap repair for tongue cancer patients needs to be further standardized, and needs to carry out evidence-based clinical research.
ABSTRACT
More and more evidences have revealed that intestinal microbiota play an important role in heart failure in the elderly.This article is aimed to discuss the correlations between intestinal microecology with the development and progression of heart failure in the elderly, to explore the complexity and interaction between intestinal microecology and heart failure from the changes of intestinal microecology, to prove whether supplementing probiotics can improve symptoms of heart failure in elderly, and to analyze the potential of probiotics as a new therapeutic target for heart failure in the elderly.
ABSTRACT
Objective To understand the multi-leadership style and nurses'innovative efficacy in operating room nurses, and to analyze the influence of multiple leadership styles of nurses on nurses'innovative efficacy and to provide reference for nurses to improve their efficacy. Methods From March to April 2017, 200 nurses from two operating rooms, Grade-A Hospital, Taiyuan, Shanxi Province and two Grade- 3 A hospitals, were selected for convenience of sampling. The general information questionnaire, Multiple leadership style scale, innovative efficacy scale questionnaire. Results The nurses' perceived head nurses'changeable leadership style score was (54.34 ± 11.78) points and the transactional leadership style score was (34.12 ± 7.32) points respectively. The total score of nurses'innovative efficacy was (72.32 ± 14.53), (3.44 ± 0.69) points. The factors influencing nurses'innovative efficacy were the motivation, intellectual stimulation and personalized care in the multi-leadership style of nurse in the operating room (t=4.962, 2.846, 2.796, P <0.05). Conclusions The multi-leadership style of operating room nurse chief can play an active role in promoting the innovation efficacy of operating room nurses. The head nurse should pay attention to cultivating the leadership qualities of diversified leadership style, promote the innovation efficacy of operating room nurses and improve the operating room Nursing service quality.
ABSTRACT
Objective To analyze dietary pattern and its seasonal characteristic in residents of Shanghai.Methods A representative sample was surveyed and followed up in four different seasons during 2012-2014.Information of food consumption was collected by 3-day 24-hour dietary recall and condiments weighing method.The intake of energy and macronutrients were calculated according to the Chinese Food Composition Table.Results Annual average daily intake was 191.09 g for grain,250.69 g for vegetable,107.23 g for fruit,223.53 g for animal food,96.39 g for dairy products,11.19 g for soy bean and its product,36.54 g for cooking oil,and 7.57 g for salt.Significant differences were observed in food consumption among different seasons (P＜0.05).Annual average daily intake of energy was 2 048.70 kcal,carbohydrates was 241.04 g,protein was 82.35 g,fat was 85.99 g and proportion of energy contributed by fat was 37.99％.Different seasons,age,residential areas,and income were influence factors for food consumption (P＜0.05).Conclusions Energy intake was adequate in residents of Shanghai.Unhealthy dietary pattem,including high proportion of fat and consumption of cooking oil and salt (lower than average intake of large cities,but higher than recommendation),was observed.It is necessary to take appropriate nutrition intervention,and future researches on dietary intake should consider seasonal influences.
ABSTRACT
Objective To analyze dietary pattern and its seasonal characteristic in residents of Shanghai.Methods A representative sample was surveyed and followed up in four different seasons during 2012-2014.Information of food consumption was collected by 3-day 24-hour dietary recall and condiments weighing method.The intake of energy and macronutrients were calculated according to the Chinese Food Composition Table.Results Annual average daily intake was 191.09 g for grain,250.69 g for vegetable,107.23 g for fruit,223.53 g for animal food,96.39 g for dairy products,11.19 g for soy bean and its product,36.54 g for cooking oil,and 7.57 g for salt.Significant differences were observed in food consumption among different seasons (P＜0.05).Annual average daily intake of energy was 2 048.70 kcal,carbohydrates was 241.04 g,protein was 82.35 g,fat was 85.99 g and proportion of energy contributed by fat was 37.99％.Different seasons,age,residential areas,and income were influence factors for food consumption (P＜0.05).Conclusions Energy intake was adequate in residents of Shanghai.Unhealthy dietary pattem,including high proportion of fat and consumption of cooking oil and salt (lower than average intake of large cities,but higher than recommendation),was observed.It is necessary to take appropriate nutrition intervention,and future researches on dietary intake should consider seasonal influences.
ABSTRACT
Objective To explore the molecular basis of the characteristics of the serum auto-fluorescent spectrum in patients with ovarian cancer and the changes that might be induced by surgery. Methods Using fluorospectrophotometer and 300nm excitation light,the serum auto-fluorescent spectrum of 84 patients with ovarian cancer before and after the surgery and 30 healthy people were detected. Meanwhile, the serum tumor signs (CEA, CA199 and CA125), hemoglobin and plasma albumin level of all patients with ovarian cancer were detected. Their correlation with the fluorescence spectral characteristic parameters were analyzed. Results Compared with healthy people, the serum auto-fluorescent spectrum in patients with ovarian cancer exhibited purple-shifted position of λ2 peak and red shift in λ4 peak,had higher peak extent inλ1, λ2, λ4 and λ6 peak, and larger peak area of λ1, λ2, λ3, λ4 and λ6. Compared with those in ovarian cancer patients before surgery, the serum auto-fluorescent spectrum in these patients after operation had red shifts in λ1, λ2 and λ3 peak, lower peak extent in λ1, λ2, λ3 and λ4, and smaller peak area in λ1, λ2, λ3,λ4 and λ6 peak. In ovarian cancer patients, the serum level of CEA was positively correlated with the λ2 peak extent and the peak area of λ2 and λ3, while the serum level of CA125 was positively correlated with the peak extent of λ1-λ4 and λ6 and the peak area of λ1-λ3. The serum level of CA199 was negatively correlated with the λ2 position and positively correlated with the peak extent of λ1-λ6 and the peak area ofλ1-λ3 and λ6 in patents with ovarian cancer. Besides, the serum albumin was positively correlated with theλ2 peak position and negatively with the peak extent of λ1-λ6 and the λ1-λ3 peak area, while the level of hemoglobin was positively correlated with λ1 peak position. Conclusions The elevated serum tumor markers and lower albumin (plasma) level lead to the changes of the serum autofluorescence spectra characteristic parametersin in patients with ovarian cancer.These changes can be modestly corrected by surgery.
ABSTRACT
Objectives To investigate the mutational characteristics of MSX1 and PAX9 genes in a family affected by non-syndromic oligodonti so as to study the pathogenesis of oligodontia from a molecular prospective. Methods A family with oligodontia, but of different descent and unrelated healthy controls were enrolled in our study. Genomic DNA was isolated from the blood samples. Mutation analyses were performed by amplifying MSX1 and PAX9 exons and sequencing the products. Results DNA sequencing revealed a novel missense mutation c.348C>T in a highly conserved homeobox sequence of MSX1 and a known polymorphisms c. 469+35- c.469+45del in exon 1 and in intron in the two patients and in two unrelated healthy controls. But we did not detect any mutation in PAX9. Conclusion Our finding suggests the samesense mutation (c.348C>T) and the polymorphisms (c.469+35- c.469+45del) may be responsible for oligodontia phenotype in this Chinese family.
ABSTRACT
Botryococcus braunii is a unique colonial green microalga and a great potential renewable resource of liquid fuel because of its ability to produce lipids. Due to the dense cell colonies and rigidly thick cell wall of B. braunii, the traditional Nile red method is usually of low sensitivity and bad repeatability and hard for the determination of lipid content in the cells. By dispersing the colony with ultrasonic, assisting permeation of Nile red across the cell wall with dimethyl sulfoxide and optimizing the staining conditions, we established an improved detection method. The details were as follows: after the colonial algal sample was treated by ultrasonic at 20 kHz for 20 s, 100 W transmitting power and with 1 s on/1 s off intermittent cycle, the equivoluminal 15% (V/V) dimethyl sulfoxide and 3 microg/mL Nile red were successively added and mixed evenly, then the staining system was incubated in dark at 40 degrees C for 10 min, and subsequently was measured by fluorescence spectroscopy detection with an excitation wavelength of 490 nm. Compared with the traditional method, the improved one not only had higher detection sensitivity which was increased by 196.6%, but also had obviously better detection repeatability whose characteristic parameter - relative standard deviation (RSD) was decreased from 10.91% to 1.84%. Therefore, the improved method could provide a rapid and sensitive detection of lipid content for B. braunii breeding and cultivation.
Subject(s)
Chlorophyta , Chemistry , Lipids , Microalgae , Chemistry , Spectrometry, Fluorescence , Methods , UltrasonicsABSTRACT
Objective:To investigate the association of single nucleotide polymorphism in mdr1C3435T with susceptibility to ovarian cancer.Methods:mdr1C3435T gene was determined by PCR-RFLP method in 135 ovarian cancer patients(cases group) and 146 healthy women(control group).Results:The frequency of C/C genotype in cases group(30.37%) was significantly lower than that in controls(47.95%),and the frequencies of T/C(44.44%)and T/T(25.19%) respectively in cases group were significantly higher than those in controls(T/C38.35%,T/T13.7%).The frequency of mdr1C3435T null genotype in cases group(25.19%) was higher than that in control group(13.7%).Compared with controls,the susceptibility of ovarian cancer with the T/T genotype was increased by 2.121-fold.There was significant difference in frequency of mdr1C3435T null and nonnull genotype between cases and controls of average age(P
ABSTRACT
OBJECTIVE:To prepare tamsulosin hydrochloride sustained-released tablets and to investigate the in vitro drug release feature and mechanism of the tablets.METHODS:Tamsulosin hydrochloride sustained-released tablets were prepared with HPMC as matrix.The effects of multiple factors on the in vitro drug release rate were evaluated with accumulative release rate as index,and the slow release feature was compared between the sustained tablets and the common tablets.RESULTS:The drug release rate was predominantly influenced by the amount of HPMC and the tablet compressing pressure,and the optimum values of the two were 25% and 8kg～11kg,respectively.The accumulative release rates of the sustained tablets and the common tablets at 0.5h were 10% and 50%,respectively.CONCLUSIONS:The sustained-release tablets had a better sustained release efficacy as compared with common tablets,and the drug release feature of the sustained tablets was the synergistic action of the tamsulosin hydrochloride diffusion and matrix degradation and the release pattern obeyed non-Fick diffusion mechanism.
ABSTRACT
Objective:To construct a recombination plasmid containing a kanamycin resistance gene,the upstream and downstream fragment of luxS of Streptococcus mutans so that luxS can be knock out by transforming the plasmid into S.mutans later.Methods:Kanamycin resistance gene,the upstream and downstream of luxS were cloned respectively by using plasmid pEGFP-N1 and DNA of Streptococcus mutans as template.Then the genes were ligated into Multiple Cloning Site(MCS) of vector pMD19-T in certain order and transformed into E.coli Competent Cells.Finally transformants were selected for resistance to kanamycin and ampicillin.Results:Kanamycin resistance gene and the upstream and downstream of luxS were successfully ligated into accurate enzyme digestion site of vector pMD19-T,and restriction digests analysis and sequencing result was correct.Conclusion:LuxS gene knock-out of Streptococcus Mutans recombinant plasmid is constructed and built a base of constructing Streptococcus Mutans luxS mutans in the future.
