Subject(s)
Humans , Male , Child, Preschool , Child , Food Hypersensitivity/diagnosis , Fabaceae/adverse effects , Skin TestsSubject(s)
Allergens/metabolism , Anaphylaxis/diagnosis , Hypersensitivity/diagnosis , Plant Oils/metabolism , Skin/pathology , Administration, Topical , Adult , Angioedema , Female , Humans , Hypotension , PruritusSubject(s)
Humans , Female , Adult , Anaphylaxis/diagnosis , Anaphylaxis/etiology , Insect Repellents/adverse effects , DEET/adverse effects , Skin TestsSubject(s)
Actins/immunology , Allergens/immunology , Dermatitis, Allergic Contact/diagnosis , Fish Proteins/immunology , Skin/pathology , Adult , Animals , Bass , Carps , Cross Reactions , Edema , Humans , Immunoglobulin E/metabolism , Male , Young AdultABSTRACT
No disponible
Subject(s)
Humans , Male , Young Adult , Food Hypersensitivity/diagnosis , Fish Products/adverse effects , Electrophoresis, Polyacrylamide Gel , Blotting, Western , Skin TestsSubject(s)
Allergens/adverse effects , Araceae/adverse effects , Hypersensitivity/etiology , Adult , Female , Flowers , Humans , Plant Leaves , Plant StemsABSTRACT
No disponible
Subject(s)
Humans , Female , Adult , Allergens/adverse effects , Araceae/adverse effects , Hypersensitivity/etiology , Flowers , Plant Leaves , Plant StemsABSTRACT
No disponible
Subject(s)
Humans , Animals , Female , Adult , Allergens/immunology , Conjunctivitis, Allergic/diagnosis , Conjunctivitis, Allergic/immunology , Pediculus/immunology , Rhinitis, Allergic/diagnosis , Rhinitis, Allergic/immunology , Serologic Tests , Skin TestsSubject(s)
Nut Hypersensitivity/etiology , Female , Humans , Middle Aged , Nut Hypersensitivity/diagnosis , Skin TestsABSTRACT
No disponible
Subject(s)
Female , Humans , Middle Aged , Nut Hypersensitivity/diagnosis , Nut Hypersensitivity/immunology , Hypersensitivity, Immediate/immunology , Skin Tests/methods , Nut Hypersensitivity/therapy , Food Hypersensitivity , Electrophoresis, Polyacrylamide Gel/methods , Blotting, Western/methodsABSTRACT
BACKGROUND: Our group previously found that up to 7% of amateur anglers in Caceres, Spain may be allergic to the larvae of Protophormia terraenovae (order Diptera, family Calliphoridae) used as live bait for fishing. OBJECTIVE: To identify the pattern of major allergens in P terraenovae and other species of Calliphoridae. MATERIALS AND METHODS: Extracts of P terraenovae, Calliphora vomitoria, Lucilia sericata and Lumbricus terrestris were characterized using sodium dodecyl sulfate polyacrylamide gel electrophoresis and IgE-immunoblotting techniques in individual sera from 24 patients with a positive skin test result and/or specific IgE determination (enzyme-linked immunosorbent assay [ELISA]) to P terraenovae. ELISA and IgE-immunoblotting inhibition studies were also performed to identify potential cross-reactive allergens between these species. RESULTS: IgE-immunoblotting with P terraenovae showed a band of 15.3 kDa recognized by 15 patients, in addition to 2 further allergens of 22.8 kDa and 69 kDa. For C vomitoria, 5 bands of 73, 46, 40, 28, and 14 kDa were observed. For L sericata, 2 major allergens of 73 kDa and 14 kDa were observed. In the case of L terrestris, IgE from 13 patients recognized 1 allergen of around 15.5 kDa. IgE-immunoblotting and ELISA inhibition revealed the presence of cross-reactivity, mainly between L terrestris and P terraenovae. CONCLUSIONS: P terraenovae appears to have species-specific allergens and allergens shared with C vomitoria and L sericata. Striking immunological cross-reactivity was observed between P terraenovae and L terrestris. An allergen of 15-16 kDa could be involved in this phenomenon.
Subject(s)
Allergens/immunology , Diptera/immunology , Hypersensitivity/immunology , Larva/immunology , Oligochaeta/immunology , Animals , Blotting, Western , Cross Reactions , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Humans , SpainABSTRACT
Antecedentes: La larva de Protophormia terraenovae, utilizada como cebo vivo para la pesca, es capaz de producir reacciones alérgicas en el 7% de la población de pescadores de agua dulce de Cáceres, según las observaciones previas de nuestro grupo. Objetivo: Identificar el patrón de alérgenos en P. terranovae y otros Califóridos. Materiales y métodos: Los extractos de P. terraenovae, Calliphora vomitoria, Lucilia sericata y Lumbricus terrestris se sometieron a técnicas de SDS-PAGE e IgE-immunoblotting utilizando sueros individuales de 24 pacientes sensibilizados a P. terraenovae. Se realizaron también técnicas de ELISA e IgE-immunoblotting inhibition para la identificación de posibles alérgenos comunes entre dichas especies. Resultados: 15 pacientes reconocieron una banda entorno a los 15.3 kDa frente al extracto de P. terraenovae, además de otros 2 alérgenos de 22.8 and 69kDa. Con C. vomitoria, se observaron 5 bandas de 73, 46, 40, 28 y 14 kDa. Con L. sericata se observaron 2 alérgenos mayores de 73 y 14 kDa. Usando L terrestris, 13 pacientes reconocieron un alérgeno de unos 15.5 kDa. Los estudios de inhibición IgE demostraron la presencia de reactividad cruzada inmunológica principalmente entre L. terrestris y P. terraenovae. Conclusiones: La larva de P. terraenovae parece tener alérgenos especie-específi cos y alérgenos compartidos con C. vomitoria y L. sericata. Se ha observado una importante reactividad cruzada inmunológica entre P. terraenovae y L. terrestris. Un alérgeno entre los 15-16 kDa podría ser uno de los responsables de este fenómeno (AU)
Background: Our group previously found that up to 7% of amateur anglers in Caceres, Spain may be allergic to the larvae of Protophormia terraenovae (order Diptera, family Calliphoridae) used as live bait for fishing. Objective: To identify the pattern of major allergens in P terraenovae and other species of Calliphoridae. Materials and Methods: Extracts of P terraenovae, Calliphora vomitoria, Lucilia sericata and Lumbricus terrestris were characterized using sodium dodecyl sulfate polyacrylamide gel electrophoresis and IgE-immunoblotting techniques in individual sera from 24 patients with a positive skin test result and/or specific IgE determination (enzyme-linked immunosorbent assay [ELISA]) to P terraenovae. ELISA and IgE-immunoblotting inhibition studies were also performed to identify potential cross-reactive allergens between these species. Results: IgE-immunoblotting with P terraenovae showed a band of 15.3 kDa recognized by 15 patients, in addition to 2 further allergens of 22.8 kDa and 69 kDa. For C vomitoria, 5 bands of 73, 46, 40, 28, and 14 kDa were observed. For L sericata, 2 major allergens of 73 kDa and 14 kDa were observed. In the case of L terrestris, IgE from 13 patients recognized 1 allergen of around 15.5 kDa. IgE-immunoblotting and ELISA inhibition revealed the presence of cross-reactivity, mainly between L terrestris and P terraenovae. Conclusions: P terraenovae appears to have species-specific allergens and allergens shared with C vomitoria and L sericata. Striking immunological cross-reactivity was observed between P terraenovae and L terrestris. An allergen of 15-16 kDa could be involved in this phenomenon (AU)
Subject(s)
Animals , Oligochaeta/immunology , Allergens/analysis , Allergens/chemistry , Allergens , Desensitization, Immunologic , Food Hypersensitivity/immunology , Hypersensitivity/diagnosis , Hypersensitivity/immunology , Fishes/immunology , Allergens/immunology , Electrophoresis, Polyacrylamide Gel/methods , Electrophoresis, Polyacrylamide Gel/standards , Electrophoresis, Polyacrylamide Gel , Blotting, Western/methods , Blotting, Western/standards , Blotting, Western , Enzyme-Linked Immunosorbent Assay/instrumentation , Enzyme-Linked Immunosorbent Assay/methods , Enzyme-Linked Immunosorbent AssayABSTRACT
No disponible
