Quantitative microbial risk assessment of gastrointestinal illness due to recreational exposure to E. coli and enterococci on the southern coasts of the Caspian Sea.

Background: Gastrointestinal illness refers to a broad range of diseases that affect the digestive system, including infections caused by bacteria, viruses, and parasites. Quantitative Microbial Risk Assessment (QMRA) is a powerful tool used to evaluate the risks associated with microbial pathogens in various environments. The main objective of this study was to conduct a quantitative assessment of gastrointestinal illnesses that occur as a result of exposure to E. coli and enterococci during recreational activities on the southern coasts of the Caspian Sea. Methods: Samples were collected from the recreational beaches along the border line of the Caspian Sea. The samples were analyzed for the presence and enumeration of E. coli and enterococci using the microplate method and membrane filtration techniques. Then, the annual and daily infection risks were computed using the Monte Carlo simulation approach. Results: The results revealed that the risk of daily and annual infections on the coasts of Babolsar was higher than that on the coasts of Sari. Also, in the recreational waters of these beaches, the risk of infection by enterococci was higher than that posed by E. coli. In Babolsar, the average annual infection risk caused by E. coli and enterococci was 0.365 and 1 for children and 0.181 and 0.986 for adults. Also, in Sari, the average annual infection risk caused by E. coli and enterococci was 0.060 and 0.908 for children and 0.027 and 0.815 for adults. In addition, children were more likely than adults to become infected. Conclusion: In light of the study's findings, due to the entry of untreated urban wastewater into the southern part of the Caspian Sea (northern Iran) and the high risk of infectious diseases for children, more control and health measures are necessary for children's swimming.

Bottled water safety evaluation: A comprehensive health risk assessment of oral exposure to heavy metals through deterministic and probabilistic approaches by Monte Carlo simulation.

The consumption of bottled water has witnessed substantial global expansion in recent times. This study aimed to quantitatively evaluate the concentrations of eight heavy metals (As, Ba, Cd, Cr, Mn, Mo, Ni, and Zn) in 71 high-consumption bottled water brands in Iran. Non-carcinogenic and carcinogenic risk assessments were conducted using both deterministic and probabilistic approaches. Point estimation utilizing the Hazard Quotient (HQ) formula and sensitivity analysis employing the Monte Carlo Simulation (MCS) method through 10,000 repetitions in Oracle Crystal Ball® was used to ascertain the health risks associated with heavy metal exposure. Heavy metal concentrations were quantified through Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). HQ point estimation results indicated that Cr exhibited the highest mean HQ value, whereas Cd demonstrated the lowest. In the probabilistic approach, the highest 95 percentile values were observed for Cr and Mo at 3.9E-01, while the lowest values were recorded for Cr and Mn at 1.10E-02. Heavy metal concentrations emerged as critical factors influencing non-carcinogenic and carcinogenic risks across all groups in the sensitivity analysis. The findings highlight the need for ongoing monitoring, research, and targeted regulations to address health risks from heavy metal exposure in bottled water and ensure public well-being.

Occurrence of microplastics in edible tissues of livestock (cow and sheep).

Plastic contamination is widely recognized as a major environmental concern due to the entry of small plastic particles into the food chain, thereby posing potential hazards to human health. However, the current understanding of microplastic (MP; < 5 mm) particles in livestock, which serve as an important food source, is limited. This study aims to investigate the concentration and characteristics of MPs in edible tissues of cow and sheep, namely liver, meat, and tripe, obtained from butcher shops in five areas of Bushehr port, Iran. The mean concentration of MPs in different tissues of cow and sheep were 0.14 and 0.13 items/g, respectively. Among the examined tissues, cow meat exhibited the highest concentration of MPs, with a concentration of 0.19 items/g. Nylon and fiber were identified as the predominant polymer types and shapes of MPs found in cow and sheep tissues, respectively. Furthermore, no statistically significant difference was observed in MP concentration across different tissues of cow and sheep. Significantly, this study highlights the elevated hazards associated with exposure to MPs through the consumption of edible cow and sheep tissues, particularly for children who consume meat. The results underscore the potential transfer of MPs from the environment to livestock bodies through their food, contamination during meat processing, and subsequent health hazards for consumers.

Machine learning-based prediction of effluent total suspended solids in a wastewater treatment plant using different feature selection approaches: A comparative study.

Accurately predicting the characteristics of effluent, discharged from wastewater treatment plants (WWTPs) is crucial for reducing sampling requirements, labor, costs, and environmental pollution. Machine learning (ML) techniques can be effective in achieving this goal. To optimize ML-based models, various feature selection (FS) methods are employed. This study aims to investigate the impact of six FS methods (categorized as Wrapper, Filter, and Embedded methods) on the accuracy of three supervised ML algorithms in predicting total suspended solids (TSS) concentration in the effluent of a municipal wastewater treatment plant. Based on the features proposed by each FS method, five distinct scenarios were defined. Within each scenario, three ML algorithms, namely artificial neural network-multi layer perceptron (ANN-MLP), K-nearest neighbors (KNN), and adaptive boosting (AdaBoost) were applied. The features utilized for predicting TSS concentration in the WWTP effluent included BOD5, COD, TSS, TN, NH3 in the influent, and BOD5, COD, residual Cl2, NO3, TN, NH4 in the effluent. To construct the models, the dataset was randomly divided into training and testing subsets, and K-fold cross-validation was employed to control overfitting and underfitting. The evaluation metrics that are used are root mean squared error (RMSE), mean absolute error (MAE), and correlation coefficient (R2). The most efficient scenario was identified as Scenario IV, with the Sequential Backward Selection FS method. The features selected by this method were CODe, BOD5e, BOD5i, TNi. Furthermore, the ANN-MLP algorithm demonstrated the best performance, achieving the highest R2 value. This algorithm exhibited acceptable performance in both the training and testing subsets (R2 = 0.78 and R2 = 0.8, respectively).

Association metrological factors with Covid-19 mortality in Tehran, Iran (2020-2021).

The outbreak of the Coronavirus disease (COVID-19) has raised questions about the potential role of climate and environmental factors in disease transmission. This study examined meteorological and demographic factors to determine their impact on mortality and hospitalization rates in Tehran, Iran from January 1, 2021, to December 31, 2022. Notably, hospitalization cases were positively associated with temperature (P-value: 0.001 in spring, P-value: 0.045 in winter) and pressure (P-value: 0.004 in spring), while being negatively associated with wind speed (P-value: 0.03 in spring, P-value: 0.01 in autumn) and humidity (P-value: 0.001 in autumn) during the spring and autumn seasons. Conversely, mortality was associated with wind speed (P-value: 0.01) and pressure (P-value: 0.02) during winter and spring, respectively. Moreover, temperature was associated with mortality in both spring (P-value: 0.00) and winter (P-value: 0.04). The findings suggest that identifying the environmental factors that contribute to the spread of COVID-19 can help prevent future waves of the pandemic in Tehran.

Lactational Exposure of Human Infants to Metal (loid)s: A Comparison of Industrial and Urban Inhabitants in North of the Persian Gulf.

In this study, postnatal metal (loid)s (MLs) exposure was compared between the petrochemical and gas area of Asaluyeh (PGA) and urban area of Kaki (UA) in Bushehr province, Iran. Two hundred human breast milk (BM) samples from the industrial and urban areas were analyzed for MLs using Inductivity Coupled Plasma-Optical Emission Spectrometry (ICP-OES). Boron (B), copper (Cu), iron (Fe), and nickel (Ni) were found at the highest levels in both study areas. Adjusted multiple linear regression models revealed that the mean concentration of total MLs in BM samples collected from the PGA was statistically significantly greater than that of the UA (655.85 vs. 338.17 µg/L). Also, the mean concentrations of all detected MLs in BM samples collected from the PGA were statistically significantly higher than those collected from the UA. The hazard index (HI) of combined MLs in the PGA and UA illustrated non-cancer risk for infants. Lead (Pb) and chromium (Cr) in the PGA and Cr in the UA showed the risk of cancer. So it can be concluded that nursing infants from an industrial area are most at risk for MLs exposure during entire lactation course than those from an urban area.

Analysis of microplastics in ships ballast water and its ecological risk assessment studies from the Persian Gulf.

Transport of ballast water is considered a significant vector for dispersion of different pollutants, including microplastics (MPs), throughout the world's oceans. However, there is limited information on MPs in ballast water. Size distribution, polymer type, and ecological risks of MPs in ballast water were investigated for the first time in this study. The mean levels of MPs in ballast water and seawater samples were 12.53 and 11.80 items/L, respectively. MPs with a size category of 50-300 µm was the most abundant. Fiber, black, and polycarbonate (PC) were the predominant shape, color, and polymer type of identified MPs in ballast water and seawater, respectively. The pollution load index (PLI), hazard index (HI), and risk quotient (RQ) indicated high levels of MP pollution, potentially indicating an ecological risk. These findings increase our understanding of the major sources (such as ballast water), transportation routes, and related ecological risks of MPs to marine ecosystems.

Health risk assessment of workers exposed to lead dust in informal e-waste recycling workshops.

Informal recycling of electronic waste (e-waste) has attracted significant attention due to its economic benefits and rapid growth rate in many parts of the world. Unfortunately, unsafe conditions of recycling workshops possess chronic exposure to workers and lead to elevated blood lead concentrations (BLCs). Upon measuring the lead concentration in the dust of recycling workshops and the e-wastes in southwestern region of Iran, the related health risks were assessed in 30 exposed workers and 30 non-exposed habitants cases based on the determination of BLC. The average BLCs in exposed workers and non-exposed habitants cases were 24 µg/dL and 7 µg/dL, respectively. The geo-accumulation index (Igeo) revealed heavy contamination of dust in informal e-waste recycling workshops (IERWs) (5023 µg/kg) and significantly lower levels in unexposed areas (49 µg/kg). Health risk assessment indicated that lead exposure from IERWs appears to be a potential threat to workers and indirectly to their families.

Microplastics and phthalate esters release from teabags into tea drink: occurrence, human exposure, and health risks.

Tea, the most common infusion worldwide, is usually sold in teabags due to the ease of usage. The increase in the use of plastic materials in the food packaging industry has led to an increase in released contaminants, such as microplastics (MPs) and phthalates (PAEs), in various food products including teabags. In this research, the abundance and features of MPs as well as PAEs concentration were investigated in 45 teabag samples of different Persian and German brands. The abundance of MPs in the Persian and German teabag samples was averagely 412.32 and 147.28 items/single teabag, respectively. Also, average PAEs levels in the Persian and German teabag samples were 2.87 and 2.37 mg/g, respectively. The predominant size category of MPs was related to 100-250 µm. Fibers and transparent were the dominant shape and color of detected MPs in teabags, respectively. Polyethylene (PE) and nylon were the most common MP polymer types. The most prominent PAEs congeners in teabag samples were diethylhexyl phthalate (DEHP) and diisobutyl phthalate (DiBP). Furthermore, MP exposure hazard through tea ingestion was analyzed for children and adults. DEHP showed the cancer risk (CR) for children and adults. The findings of this research indicated that high MPs and PAEs levels are released from teabags into tea drinks. Considering a daily drinking of a volume of 150 and 250 mL tea by children and adults, 486 and 810 MPs may enter their bodies, respectively. Thus, tea prepared with teabag-packed herbs may pose a significant health risk for consumers.

Burden of injury due to occupational accidents and its spatiotemporal trend at the national and subnational levels in Iran, 2011-2018.

PURPOSE: The spatiotemporal trend of the burden of injury due to occupational accidents in Iran, 2011-2018 were assessed at the national and subnational levels. METHODS: The burden of occupational injury was estimated using three datasets of occupational injury data, the employed population, and duration and disability weight of injuries. RESULTS: The disability-adjusted life years (DALYs), deaths, DALY rate, and death rate (per 100,000 workers) of occupational injury in Iran drastically decreased from 169,523, 2,280, 827, and 11 in 2011 to 86,235, 1,151, 362, and 5 in 2018, respectively. The DALY rates of occupational injury were significantly different by gender and age in a manner that the DALY rate of men was much higher than that of women and the DALY rates by age group in 2018 ranged from 98 for 50 y and over to 901 for 15-19 y. The shares of injury outcomes in the total DALYs in 2018 were as follows: 63.6% for fatal injuries, 17.4% for fracture, 7.9% for open wound, 7.3% for amputation, and 3.8% for other injuries. Over 83% of the DALYs was observed in three economic activity groups of construction, manufacturing, and community, social, and personal service activities. The three provinces with the highest DALY rates in 2018 were Markazi, West Azarbaijan, and East Azarbaijan, respectively. CONCLUSION: Despite the decreasing temporal trend, the burden of occupational injury in Iran in 2018 was high. The high-risk groups and hot spot provinces should be taken into more consideration for further reduction of the injury burden.

Assessment of health risk and burden of disease induced by exposure to benzene, toluene, ethylbenzene, and xylene in the outdoor air in Tehran, Iran.

The health risk and burden of disease induced by exposure to benzene, toluene, ethylbenzene, and xylene (BTEX) in the outdoor air in Tehran, 2019 were assessed based on the data of five fixed stations with weekly BTEX measurements. The non-carcinogenic risk, carcinogenic risk, and disease burden from exposure to BTEX compounds were determined by hazard index (HI), incremental lifetime cancer risk (ILCR), and disability-adjusted life year (DALY), respectively. The average annual concentrations of benzene, toluene, ethylbenzene, and xylene in the outdoor air in Tehran were 6.59, 21.62, 4.68, and 20.88 µg/m3, respectively. The lowest seasonal BTEX concentrations were observed in spring and the highest ones occurred in summer. The HI values of BTEX in the outdoor air in Tehran by district ranged from 0.34 to 0.58 (less than one). The average ILCR values of benzene and ethylbenzene were 5.37 × 10-5 and 1.23 × 10-5, respectively (in the range of probable increased cancer risk). The DALYs, death, DALY rate (per 100,000 people) and death rate (per 100,000 people) induced by BTEX exposure in the outdoor air in Tehran were determined to be 180.21, 3.51, 2.07, and 0.04, respectively. The five highest attributable DALY rates in Tehran by district were observed in the districts 10 (2.60), 11 (2.43), 17 (2.41), 20 (2.32), and 9 (2.32), respectively. The corrective measures such as controlling road traffic and improving the quality of vehicles and gasoline in Tehran could reduce the burden of disease from BTEX along with the health effects of other outdoor air pollutants.

Implemented indoor airborne transmission mitigation strategies during COVID-19: a systematic review.

The COVID-19 pandemic has inflicted major economic and health burdens across the world. On the other hand, the potential airborne transmission of SARS-COV-2 via air can deeply undermine the effectiveness of countermeasures against spreading the disease. Therefore, there is an intense focus to look for ways to mitigate the COVID-19 spread within various indoor settings. This work systematically reviewed articles regarding airborne transmission of SARS-COV2 in various indoor settings since the onset of the pandemic. The systematic search was performed in Scopus, Web of Science, and PubMed databases and has returned 19 original articles carefully screened with regard to inclusion and exclusion criteria. The results showed that the facilities, such as dormitories and classrooms, received the most attention followed by office buildings, healthcare facilities, residential buildings, and other potential enclosed spaces such as a metro wagon. Besides, the majority of the studies were conducted experimentally while other studies were done using computer simulations. United States (n = 5), Spain (n = 4) and China (n = 3) were the top three countries based on the number of performed research. Ventilation rate was the most influential parameter in controlling the infection spread. CO2 was the primary reference for viral spread in the buildings. The use of natural ventilation or a combination of mechanical and natural ventilations was found to be highly effective in the studies. The current work helps in furthering research on effective interventions to improve indoor air quality and control the spread of COVID-19 and other respiratory diseases. Supplementary information: The online version contains supplementary material available at 10.1007/s40201-023-00847-0.

Carcinogenic and non-carcinogenic risk assessment of exposure to trace elements in groundwater resources of Sari city, Iran.

The daily intake of trace elements through water resources and their adverse health effects is a critical issue. The purpose of this research was to assess the carcinogenic and non-carcinogenic risks of exposure to iron (Fe), copper (Cu), manganese (Mn), zinc (Zn), chromium (Cr), lead (Pb), and arsenic (As) in groundwater resources of Sari city, Iran. The concentrations of the trace elements in a total number of 66 samples from the groundwater sources were measured using inductively coupled plasma mass spectrometry (ICP-MS). The hazard index (HI) levels of exposure to the trace elements from the groundwater sources for adults, teenagers, and children were 0.65, 0.83, and 1.08, respectively. The carcinogenic risk values of Cr and As in the groundwater sources for children, teenagers, and adults were 0.0001, 0.00009, 0.00007, 0.0003, 0.0002, and 0.0001, respectively, causing a total carcinogenic risk value higher than the acceptable range, and removing Cr and As from the groundwater resources is recommended for safe community water supply.

Toxic effect of cigarette butts leachates on blood markers of Periophthalmus waltoni species from the Persian Gulf region.

Cigarette butts (CBs) are one of the most common, long-lasting, and toxic forms of marine and coastal area debris. Although the significance of CBs and the toxic contents of this waste items are well recognized, but there is still a lack of information about the effects of this waste on the aquatic organisms. Therefore in this study, the in-vivo toxic effects of various CBs leachates (smoked cigarette butts with tobacco [SCBs], smoked CBs without tobacco [SFs], and unsmoked filters [USFs]) on cellular and chemical hematologic markers in fish (Periophthalmus waltoni) were evaluated. In three acute, sub-acute, and sub-chronic exposure measurements, P. waltoni exposed to different CBs leachates showed a significant increase in white blood cells, creatine kinase, lactate dehydrogenase, alkaline phosphatase, alanine transaminase, and aspartate transaminase, as well as a decrease in hemoglobin (Hb) levels. The mean ± SD values of Hb in P. waltoni species exposed to different CBs leachates (control, SCBs, SFs and USFs) in acute (1 day) phase were 7.15 ± 0.34, 6.02 ± 0.29, 6.25 ± 0.25 and 6.89 ± 0.1 g/dl respectively. These values in subacute (28 days) phase were 6.70 ± 0.15, 5.19 ± 0.24, 5.67 ± 0.30 and 6.10 ± 0.24 g/dl and in sub chronic exposure (42 days) phase were 7.20 ± 0.40, 5.16 ± 0.30, 5.88 ± 0.34 and 6.60 ± 0.33 g/dl respectively. Our results showed that CBs leachates act as a stressor, leading to changes in some hematologic markers in P. waltoni species. Because of the continued deposition of CBs waste into global aquatic environments, policies to mitigate this waste in coastal areas are needed to prevent potentially negative effects on fish and other aquatic organisms.

A comparative monitoring of maternal and cord serum polychlorinated biphenyls levels from Iranian pregnant women between industrial and urban areas.

The aim of this study was to compare maternal and fetal exposure to PCBs in pregnant women from a petrochemical and gas area (PGA) and an urban area (UA), by the analyses of serum samples from mother (MS) and cord (CS). After liquid-liquid extracting, samples were analyzed for 12 PCBs congeners by gas chromatography mass spectrometer. Adjusted multiple linear regression models showed the mean levels (µg/L) of total PCBs in the MS and CS samples from the PGA (1.70 ± 0.81 and 1.19 ± 0.43) were significantly higher than those from UA (1.64 ± 0.75 and 1.07 ± 0.38). PCB 44 was predominant in both MS and CS serum samples, and in both PGA (0.80 ± 0.70 and 0.76 ± 0.67) and UA (0.79 ± 0.39 and 0.67 ± 0.34). A negative correlation was found for PCB 52 as one-unit increase in the cord serum levels was associated with 0.024 g decrease in newborn weight. Similarly, one-unit increase in the maternal serum PCB 18 concentrations were associated with 0.09 and 0.086 cm decrease in newborn height and head circumference. The serum levels of PCB 18 and 52 in the mothers who consumed meat and milk at least 1 meal/week were higher than these who consumed meat and milk never or less than 1 meal/month. The findings in this study indicated that higher maternal exposure to PCBs, as result of living in an industrialized area, leads to higher PCBs accumulation in cord blood, which consequently passes to the developing fetus. These events may cause harmful effects on both them in-utero and afterbirth growth and development.

Assessment of cause-specific mortality and disability-adjusted life years (DALYs) induced by exposure to inorganic arsenic through drinking water and foodstuffs in Iran.

The health risk and burden of disease induced by exposure to inorganic arsenic (iAs) through drinking water and foodstuffs in Iran were assessed. The iAs levels in drinking water and foodstuffs (15 food groups) in the country were determined through systematic review of three international databases (PubMed, Scopus, and Web of Science) and meta-analysis. Based on the results of the systematic review and meta-analysis, the average iAs levels in drinking water and all the food groups at the national level were lower than the maximum permissible levels. The total average non-carcinogenic risk of dietary exposure to iAs in terms of hazard index (HI) was 3.4. The average incremental lifetime cancer risk (ILCR) values of dietary exposure to iAs were determined to be 1.5 × 10-3 for skin cancer, 1.0 × 10-3 for lung cancer, and 4.0 × 10-4 for bladder cancer. Over two-thirds of the non-carcinogenic and carcinogenic risk of dietary exposure to iAs was attributed to bread and cereals, drinking water, and rice. The total annual cancer incidence, deaths, disability-adjusted life years (DALYs), death rate, and DALY rate (per 100,000 people) were assessed to be 3347 (95 % uncertainty interval: 1791 to 5999), 1302 (697 to 2336), 72,606 (38,833 to 130,228), 1.6 (0.87 to 2.9), and 91 (49 to 160). The contribution of mortality in the attributable burden of disease was 95.1 %. The contributions of the causes in the attributable burden of disease were 72 % for lung cancer, 16 % for bladder cancer, and 12 % for skin cancer. Due to the significant attributable burden of disease, national and subnational action plans consisting of multi-disciplinary approaches for risk management of dietary exposure to iAs, especially for the higher arsenic-affected areas and high-risk population groups in the country are recommended.

Graphene oxide/MIL 101(Cr) (GO/MOF) nano-composite for adsorptive removal of 2,4-dichlorophenoxyacetic acid (2,4 D) from aqueous media: synthesis, characterization, kinetic and isotherm studies.

Contamination of water resources with various pollutants and therefore lack of clean water resources are major problems that threaten many human societies. The need to develop efficient methods and materials to decontaminate water resource is an undeniable fact. Metal-organic frameworks (MOFs), as new class of highly crystalline porous solids, have attracted a great deal of attention in different research fields, especially in adsorptive removal and purification. In this study, MIL 101(Cr) MOF decorated with graphene oxide nano-layers (GO/MOF) was synthesized by a simple one-pot hydrothermal method. Scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and electron dispersion energy (EDS) were utilized to approve the growing of Cr-MOF on graphene oxide nano-layer. The synthesized nano-composite was used as a potential adsorbent for the removal of a pesticide, 2, 4-dichlorophenoxyacetic acid (2,4 D). The adsorption performance, kinetic and mechanism of 2,4 D adsorption onto GO/MOF were studied. The highest adsorption capacities of 476.9 mg g-1 was obtained at room temperature, pH 6.0 using 0.6 gL-1 of GO/MOF which was 34% higher than that of pristine Cr-MOF. The kinetics and isotherm data fitted well with pseudo-second kinetic and Langmuir isotherm model, respectively. The reusability and stability analyses showed that the synthesized GO/MOF nanocomposite kept 89% of sorption capacities for 2,4 D after four adsorption-desorption cycles. GO/MOF nano-composite was successfully applied to remove 2,4 D from agricultural waste. The results approved that the synthesized nano-composite could introduce as a stable and high performance adsorbent for adsorptive removal of selected pesticide.

Engineered model of t(7;12)(q36;p13) AML recapitulates patient-specific features and gene expression profiles.

Acute myeloid leukaemia carrying the translocation t(7;12)(q36;p13) is an adverse-risk leukaemia uniquely observed in infants. Despite constituting up to 30% of cases in under 2-year-olds, it remains poorly understood. Known molecular features are ectopic overexpression of the MNX1 gene and generation of a fusion transcript in 50% of patients. Lack of research models has hindered understanding of t(7;12) biology, which has historically focused on MNX1 overexpression rather than the cytogenetic entity itself. Here, we employed CRISPR/Cas9 to generate t(7;12) in the human K562 cell line, and in healthy CD34+ haematopoietic progenitors where the translocation was not sustained in long-term cultures or through serial replating. In contrast, in K562 cells, t(7;12) was propagated in self-renewing clonogenic assays, with sustained myeloid bias in colony formation and baseline depletion of erythroid signatures. Nuclear localisation analysis revealed repositioning of the translocated MNX1 locus to the interior of t(7;12)-harbouring K562 nuclei - a known phenomenon in t(7;12) patients which associates with ectopic overexpression of MNX1. Crucially, the K562-t(7;12) model successfully recapitulated the transcriptional landscape of t(7;12) patient leukaemia. In summary, we engineered a clinically-relevant model of t(7;12) acute myeloid leukaemia with the potential to unravel targetable molecular mechanisms of disease.

Integrated ultrasound-assisted magnetic solid-phase extraction for efficient determination and pre-concentration of polycyclic aromatic hydrocarbons from high-consumption soft drinks and non-alcoholic beers in Iran.

In the present research, an ultrasound-assisted magnetic solid-phase extraction coupled with a gas chromatography-mass spectrometry hybrid system was developed for the extraction/determination of trace amounts of polycyclic aromatic hydrocarbons in high-consumption soft drinks and non-alcoholic beers in Iran using magnetite graphene oxide adsorbent. The magnetite graphene oxide was characterized by scanning electron microscope, transmission electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer techniques. The highest extraction recovery (73.05%-95.56%) and enrichment factor (90.65-106.38) were obtained at adsorbent mass: 10 mg, adsorption time: 30 min, salt addition: sodium chloride 10% w/v, desorption time: 20 min, eluent type: hexane: acetone (1:1, v/v), and desorption solvent volumes: 200 µl. Under optimum conditions, the linearity range for polycyclic aromatic hydrocarbons determination was 0.2-200 ng/ml with a coefficient of determination > 0.993, the limit of detection = 0.09-0.21 ng/ml, the limit of quantitation = 0.3-0.7 ng/ml, and relative standard deviation < 8.1%, respectively. Relative recoveries in spiked real samples ranged from 94.67 to 109.45% with a standard deviation < 6.05%. The proposed method is effective, sensitive, and reusable and it is promising for the analysis of polycyclic aromatic hydrocarbons residues in environmental samples.

Maternal and fetal exposure to metal (loid)s, maternal nutrition status, and impact on prenatal growth in an energy rich zone and an urban area along the Persian Gulf.

The main purposes of the study were to determine whether maternal and cord blood (MB and CB) levels of metal (loid)s (MLs) are different between industrial and urban areas. Subjects were derived from the petrochemical and gas area (PGA) of Asaluyeh County and the urban area (UA) of Bushehr County in Iran. Adjusted models revealed significant differences between the PGA and UA for maternal Fe, Cu, Mg, Cd, Zn, Ni, V and Pb levels, likewise cord Cu, V, Pb and Ni levels, which were higher in the PGA. Unadjusted models revealed there were decreases in neonatal weight and head circumference by 16.550 g and 0.059 cm, respectively for each Ln unit increase in maternal Cr levels. The mean blood levels of MLs significantly associated with food consumption frequency. The MLs were detected at higher levels in the body's fetuses and their mothers who resided in the PGA than those from the UA. So, the fetuses of mothers living in an industrial areas may be at higher risk from anthropometric measures. The impact of prenatal exposure to MLs on prenatal growth should be considered for better understanding in the further studies.