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Arch Virol ; 165(2): 463-470, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31863266

ABSTRACT

The aim of this work was the genetic typing of RVA isolates originating from pigs and human patients in Slovakia. Seventy-eight rectal swabs from domestic pigs and 30 stool samples from humans were collected. The whole VP7 (G genotypes), VP6 (I genotypes) and partial VP4 (P genotypes) ORFs were amplified by RT-PCR. Genetic variability was higher amongst porcine sequences, where four G genotypes (G3, G4, G5, G11), two P genotypes (P[6], P[13]) and one I5 genotype were detected. Human RVA strains were represented by two G genotypes (G1, G3), two I genotypes (I1, I2), and one P genotype (P[8]). Genetic analysis did not show a relationship between Slovakian porcine and human RVA strains, but phylogenetic grouping of some Slovakian porcine sequences with Hungarian human sequences in both G and P genotypes was observed.


Subject(s)
Genetic Variation , Rotavirus Infections/veterinary , Rotavirus Infections/virology , Rotavirus/classification , Rotavirus/genetics , Swine Diseases/virology , Animals , Antigens, Viral/genetics , Capsid Proteins/genetics , Feces/virology , Genotype , Humans , Rectum/virology , Reverse Transcriptase Polymerase Chain Reaction , Rotavirus/isolation & purification , Sequence Analysis, DNA , Slovakia , Sus scrofa , Swine
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