ABSTRACT
The mitotic index of hepatocytes remained unchanged after 10 intraperitoneal injections of DSLET and ACTH(4-10) in doses of 0.5, 1.5, and 5 microg/kg, but increased after injection of these substances in doses of 50 and 150 microg/kg. DSLET in doses of 5, 50, and 150 microg/kg decreased the number of antibody-producing cells in the spleen. ACTH(4-10) possessed immunosuppressive activity not only in these doses, but also in a dose of 1.5 microg/kg. As differentiated from mitotic activity of hepatocytes, the degree of immunosuppression increased with increasing the dose of test peptides.
Subject(s)
Adrenocorticotropic Hormone/pharmacology , Antibodies/metabolism , Enkephalin, Leucine/analogs & derivatives , Enkephalin, Leucine/pharmacology , Hepatocytes , Mitosis/physiology , Peptide Fragments/pharmacology , Analgesics/pharmacology , Animals , Dose-Response Relationship, Drug , Hepatocytes/drug effects , Hepatocytes/physiology , Mice , Mice, Inbred CBA , Nootropic Agents/pharmacologyABSTRACT
10-day intraperitoneal administration of the tripeptie Gly-His-Lys in doses 2.5 and 150 mu/kg raises of mitotic index of hepatocytes in acute toxic damage to the liver. In the dose 1.5 mu/kg this peptie corrects both functional activity of hepatocytes and immunological responsiveness. In a dose of 150 mu/kg the peptie has a more potent immunosuppressive action and deteriorates biochemical indices of blood serum as well as dystrophic changes in the liver.
Subject(s)
Carbon Tetrachloride/toxicity , Liver Diseases/drug therapy , Liver Diseases/immunology , Liver/drug effects , Oligopeptides/pharmacology , Animals , Chemical and Drug Induced Liver Injury , Cholestasis, Intrahepatic/drug therapy , Dose-Response Relationship, Drug , Hepatocytes/drug effects , Hepatocytes/immunology , Hepatocytes/pathology , Immunosuppressive Agents/pharmacology , Injections, Intraperitoneal , Liver/pathology , Liver Diseases/metabolism , Mice , Mice, Inbred CBA , Mitotic Index , Rats , Rats, WistarABSTRACT
Ten intraperitoneal injections of tripeptide Gly-His-Lys in doses of 1.5, 5, 50, 150, and 450 mg/kg stimulated mitotic activity of hepatocytes and dose-dependently suppressed immune reactivity (number of antibody-producing cells and delayed-type hypersensitivity reaction).
Subject(s)
Hepatocytes/drug effects , Immunosuppressive Agents/pharmacology , Oligopeptides/pharmacology , Amino Acid Sequence , Animals , Antibody Formation , Dose-Response Relationship, Immunologic , Hepatocytes/metabolism , Hypersensitivity, Delayed/prevention & control , Immunization, Passive , Immunosuppressive Agents/chemistry , Mice , Mice, Inbred CBA , Mitotic Index , Oligopeptides/chemistry , Rats , Rats, WistarABSTRACT
It was demonstrated in experiments on Wistar rats with carbon tetrachloride induced acute and chronic affection of the liver that the supernatant of the splenic cells of the poisoned animals contains 2 undialyzable factors with a molecular mass of more than 150 and 10-12 kDa. The splenocytic substances stimulate hepatocyte proliferation both in healthy allogeneic recipients and under conditions of increased proliferative activity of hepatocytes in experimental pathology of the liver.
Subject(s)
Chemical and Drug Induced Liver Injury/physiopathology , Liver Regeneration/physiology , Proteins/physiology , Spleen/chemistry , Acute Disease , Animals , Chemical and Drug Induced Liver Injury/pathology , Chronic Disease , Female , Male , Rats , Rats, Inbred StrainsABSTRACT
Administration of free and to a greater extent immobilized terrilytin to intact animals significantly enhanced the proliferative activity of hepatocytes and cells of the eye cornea under normal conditions, during physical loading and vibration. At administration of preparations of proteases splenocytes release two protein functionally different substances--a low molecular one exerting the immunostimulating effect and a relatively high molecular one enhancing the proliferative activity of nonimmunocompetent cells (hepatocytes and epitheliocytes of the cornea). The results of the studies indicate the presence of a close relationship between the immunological and nonimmunological effects caused by proteolytic enzymes.
Subject(s)
Adjuvants, Immunologic/pharmacology , Amylases/pharmacology , Enzymes, Immobilized/pharmacology , Liver/drug effects , Peptide Hydrolases/pharmacology , Physical Exertion/drug effects , Vibration/adverse effects , Animals , Cell Division/drug effects , Cornea/cytology , Cornea/drug effects , Drug Combinations/pharmacology , Immunocompetence/drug effects , Liver/cytology , Mitosis/drug effects , Molecular Weight , Rats , Rats, Inbred Strains , Spleen/cytology , Spleen/drug effects , Spleen/immunologySubject(s)
Appendicitis/physiopathology , Pain/etiology , Peritoneum/innervation , Humans , Time FactorsABSTRACT
In experiments on rabbits immunized with soluble protein antigen immune reactions were found to be accompanied by the production of lipofuscin in macrophages. This process was the morphological manifestation of the digestion of antigen by macrophages which thus acquired the ability to migrate in the organ and to form lymphoid follicules in the medullary zone of lymph nodes. The newly formed follicules seem to be the basis of pronounced specific immune response. In staphylococcal bacteriemia the phagocytic activity of macrophages was delayed, thus causing disturbances in lipofuscin production; as a result, the subsequent phases of immune response also lagged somewhat behind in time.