ABSTRACT
A new series of phosphonylated triazolo[4,5-b]pyridine (1-deaza-8-azapurine), imidazo[4,5-b]pyridine (1-deazapurine) and imidazo[4,5-b]pyridin-2(3H)-one (1-deazapurin-8-one) were synthesized from 2-chloro-3-nitropyridine and selected diethyl É·-aminoalkylphosphonates followed by reduction of the nitro group and cyclization. In the final step O,O-diethylphosphonates were transformed into the corresponding phosphonic acids. All synthesized compounds were evaluated in vitro for inhibitory activity against a broad variety of DNA and RNA viruses and their cytotoxic potencies were also established. Compound 12f showed marginal activity against cytomegalovirus Davis strain (EC50 = 76.47 µM) in human embryonic lung (HEL) cells while compounds 10g (EC50 = 52.53 µM) and 12l (EC50 = 61.70 µM) were minimally active against the varicella-zoster virus Oka strain in HEL cells. Compounds under investigation were not cytotoxic at the maximum concentration evaluated (100 µM).
Subject(s)
Acids, Acyclic/pharmacology , Antiviral Agents/pharmacology , Drug Design , Organophosphonates/pharmacology , Pyrimidine Nucleotides/pharmacology , Acids, Acyclic/chemistry , Antiviral Agents/chemical synthesis , Antiviral Agents/chemistry , Cell Line , DNA Viruses/drug effects , Humans , Microbial Sensitivity Tests , Molecular Structure , Organophosphonates/chemistry , Pyrimidine Nucleotides/chemistry , RNA Viruses/drug effectsABSTRACT
Antibiotic resistance has become one of the major threats to global health. Photodynamic inactivation (PDI) develops little antibiotic resistance; thus, it becomes a promising strategy in the control of bacterial infection. During a PDI process, light-induced reactive oxygen species (ROS) damage the membrane components, leading to the membrane rupture and bacteria death. Due to the short half-life and reaction radius of ROS, achieving the cell-membrane intercalation of photosensitizers is a key challenge for PDI of bacteria. In this work, a tetraphenylethylene-based discrete organoplatinum(II) metallacycle (1) acts as a photosensitizer with aggregation-induced emission. It self-assembles with a transacting activator of transduction (TAT) peptide-decorated virus coat protein (2) through electrostatic interactions. This assembly (3) exhibits both ROS generation and strong membrane-intercalating ability, resulting in significantly enhanced PDI efficiency against bacteria. By intercalating in the bacterial cell membrane or entering the bacteria, assembly 3 decreases the survival rate of gram-negative Escherichia coli to nearly zero and that of gram-positive Staphylococcus aureus to â¼30% upon light irradiation. This study has wide implications from the generation of multifunctional nanomaterials to the control of bacterial infection, especially for gram-negative bacteria.
Subject(s)
Acids, Acyclic/pharmacology , Anti-Bacterial Agents/pharmacology , Capsid Proteins/pharmacology , Cell Membrane/drug effects , Escherichia coli/drug effects , Gene Products, tat/pharmacology , Organoplatinum Compounds/pharmacology , Photosensitizing Agents/pharmacology , Staphylococcus aureus/drug effects , Stilbenes/pharmacology , Acids, Acyclic/chemistry , Electron Spin Resonance Spectroscopy , Escherichia coli/radiation effects , Escherichia coli/ultrastructure , Microscopy, Electron , Photochemotherapy/methods , Reactive Oxygen Species , Staphylococcus aureus/radiation effects , Staphylococcus aureus/ultrastructure , Static Electricity , Tobacco Mosaic VirusABSTRACT
In the present study, the effects of low-molecular-weight organic acids (OAs) on the toxicity of copper (Cu) to the earthworm Eisenia fetida (E. fetida) were investigated in a simulated soil solution. We exposed E. fetida to soil solution containing Cu and a variety of OAs (acetic acid, oxalic acid, citric acid, and EDTA). We found that the addition of OAs reduced the toxicity of Cu to E. fetida, where the reduction was strongest in EDTA and weakest in acetic acid. These compounds decreased the mortality rate of E. fetida that were exposed to Cu and reduced levels of antioxidant enzymes and malondialdehyde to unexposed control levels. E. fetida were exposed to Cu with OAs had reduced Cu2+, which were likely caused by Cu forming complexes with the OAs, reducing the availability of Cu. The presence of OAs also reduced Cu-induced damage on earthworm cellular ultrastructures and changed the subcellular distribution of Cu. These results demonstrated that OAs could reduce the toxicity, as well as the bioavailability, of heavy metals in soil solutions where both OAs and heavy metals often coexist.
Subject(s)
Acids, Acyclic/pharmacology , Copper/toxicity , Oligochaeta/drug effects , Soil Pollutants/toxicity , Soil/chemistry , Acids, Acyclic/chemistry , Animals , Biological Availability , Copper/metabolism , Molecular Weight , Oligochaeta/ultrastructure , Soil Pollutants/metabolism , SolutionsABSTRACT
In the present study, the effects of low-molecular-weight organic acids (LMWOAs) on the toxicity of cadmium (Cd) to Eisenia fetida were investigated in a simulated soil solution. The LMWOAs protected E. fetida from Cd toxicity, as indicated by the increased median lethal concentration (LC50) values and the increased activity of superoxide dismutase. In addition, Cd concentrations in E. fetida decreased dramatically in the presence of LMWOAs. These results were likely because of the complexation between Cd and LMWOAs, which decreased the bioavailability and consequential toxicity of Cd to E. fetida. Notably, LMWOAs reduced Cd toxicity in decreasing order (ethylenediamine tetraacetic acid [EDTA] > citric acid > oxalic acid > malic acid > acetic acid), which was consistent with the decreasing complexation constants between LMWOAs and Cd. These results advance our understanding of the interactions between Cd and LMWOAs in soil. Environ Toxicol Chem 2017;36:1005-1011. © 2016 SETAC.
Subject(s)
Acids, Acyclic/pharmacology , Cadmium/toxicity , Oligochaeta/drug effects , Soil Pollutants/toxicity , Soil/chemistry , Animals , Biological Availability , Cadmium/analysis , Cadmium/metabolism , Citric Acid/pharmacology , Malates/pharmacology , Molecular Weight , Oligochaeta/enzymology , Oligochaeta/metabolism , Oxalic Acid/pharmacology , Soil Pollutants/analysis , Soil Pollutants/metabolism , Solutions , Toxicity Tests, AcuteABSTRACT
Kanamienamide, an enamide with an enol ether, was isolated from the marine cyanobacterium Moorea bouillonii. The gross structure was established by spectroscopic analyses, and the relative stereochemistry was elucidated on the basis of the analyses of NOESY correlations and 1H-1H coupling constants. The absolute configuration was determined on the basis of the chiral HPLC analysis of the N-Me-Leu derived from kanamienamide. This is the first report of a natural product that possesses an N-Me-enamide adjacent to an enol ether. Kanamienamide showed growth-inhibitory activity toward HeLa cells with an IC50 value of 2.5 µM and induced apoptosis-like cell death.
Subject(s)
Acids, Acyclic/isolation & purification , Amides/isolation & purification , Antineoplastic Agents/isolation & purification , Apoptosis/drug effects , Aquatic Organisms/chemistry , Cyanobacteria/chemistry , Acids, Acyclic/pharmacology , Amides/pharmacology , Antineoplastic Agents/pharmacology , Cell Survival/drug effects , HeLa Cells , Humans , Inhibitory Concentration 50 , StereoisomerismABSTRACT
Candida albicans is the most important fungal pathogen of humans, causing severe infections, especially in nosocomial and immunocompromised settings. However, it is also the most prevalent fungus of the normal human microbiome, where it shares its habitat with hundreds of trillions of other microbial cells. Despite weak organic acids (WOAs) being among the most abundant metabolites produced by bacterial microbiota, little is known about their effect on C. albicans. Here we used a sequencing-based profiling strategy to systematically investigate the transcriptional stress response of C. albicans to lactic, acetic, propionic, and butyric acid at several time points after treatment. Our data reveal a complex transcriptional response, with individual WOAs triggering unique gene expression profiles and with important differences between acute and chronic exposure. Despite these dissimilarities, we found significant overlaps between the gene expression changes induced by each WOA, which led us to uncover a core transcriptional response that was largely unrelated to other previously published C. albicans transcriptional stress responses. Genes commonly up-regulated by WOAs were enriched in several iron transporters, which was associated with an overall decrease in intracellular iron concentrations. Moreover, chronic exposure to any WOA lead to down-regulation of RNA synthesis and ribosome biogenesis genes, which resulted in significant reduction of total RNA levels and of ribosomal RNA in particular. In conclusion, this study suggests that gastrointestinal microbiota might directly influence C. albicans physiology via production of WOAs, with possible implications of how this fungus interacts with its host in both health and disease.
Subject(s)
Acids, Acyclic/pharmacology , Lactic Acid/pharmacology , Transcriptome/drug effects , Candida albicans/drug effects , Candida albicans/genetics , Candida albicans/metabolism , Down-Regulation/drug effects , Iron/metabolism , RNA/biosynthesis , RNA, Ribosomal/metabolism , Up-Regulation/drug effectsABSTRACT
BACKGROUND: Our laboratory seeks a pharmacotherapeutic intervention for PKU that utilizes non-physiological amino acids (NPAAs) to block the accumulation of phenylalanine (Phe) in the brain. In previous studies (Vogel et al. 2013), methylation of the amino group of 2-aminoisobutyrate (AIB) provided an enhanced degree of selectivity for Phe restriction into the brain of Pah(enu2) mice in comparison to unmethylated AIB, leading to the hypothesis that 2-(methylamino)alkanoic acid analogs of AIB might represent targeted inhibitors of Phe accretion into the brain. METHODS: Pah(enu2) and control mice were intraperitoneally administered (500-750 mg/kg body weight, once daily; standard 19% protein diet) AIB, methyl AIB (MAIB), isovaline, and two MAIB analogs, 2-methyl-2-(methylamino)butanoic (MeVal) and 3-methyl-2-(methylamino)pentanoic (MePent) acids for one week, followed by brain and blood isolation for amino acid analyses using UPLC. RESULTS: In the brain, AIB significantly reduced Phe accretion in Pah(enu2) mice, while MeVal significantly improved glutamine and aspartic acids. Four of five test compounds improved brain threonine and arginine levels. AIB, MAIB and IsoVal significantly reduced blood Phe, with no effect of any drug intervention on other sera amino acids. CONCLUSIONS: Further evaluation of AIB and the 2-(methylamino)alkanoic acids as inhibitors of brain Phe accumulation in Pah(enu2) mice is warranted, with more detailed evaluations of route of administration, combinatorial intervention, and detailed toxicity studies.
Subject(s)
Acids, Acyclic/pharmacology , Aminoisobutyric Acids/pharmacology , Blood-Brain Barrier/metabolism , Brain/metabolism , Isoleucine/analogs & derivatives , Phenylalanine/metabolism , Phenylketonurias/drug therapy , Valine/analogs & derivatives , Acids, Acyclic/administration & dosage , Aminoisobutyric Acids/administration & dosage , Animals , Disease Models, Animal , Humans , Isoleucine/administration & dosage , Isoleucine/pharmacology , Large Neutral Amino Acid-Transporter 1/chemistry , Large Neutral Amino Acid-Transporter 1/metabolism , Methylation , Mice , Mice, Transgenic , Molecular Targeted Therapy , Organ Specificity , Phenylalanine/blood , Protein Conformation , Protein Folding , Valine/administration & dosage , Valine/pharmacologyABSTRACT
Several Clostridium spp. were incubated in a nitrogen-free medium (non-growth medium) containing only butyric acid as a sole precursor for performing butanol production by non-growing cells. Non-growing cells of Clostridium spp., especially Clostridium beijerinckii TISTR 1461, could convert butyric acid to butanol via their sole solventogenic activity. This activity was further enhanced in the presence of glucose as a co-substrate. In addition to glucose, other monosaccharides (i.e., galactose and xylose) and disaccharides (i.e., maltose, sucrose, and lactose) could also be used as a co-substrate with butyric acid. Among the organic acids tested (i.e., formic, acetic, propionic, and butyric acids), only butyric and acetic acids were converted to butanol. This study has shown that it is possible to use the non-growing cells of Clostridium spp. for direct conversion of sugars and organic acids to biobutanol. With this strategy, C. beijerinckii TISTR 1461 produced 12 g/L butanol from 15 g/L glucose and 10 g/L butyric acid with a high butanol yield of 0.68 C-mol/C-mol and a high butanol ratio of 88 %.
Subject(s)
Acids, Acyclic/metabolism , Butanols/metabolism , Clostridium/cytology , Clostridium/metabolism , Culture Media/chemistry , Disaccharides/metabolism , Monosaccharides/metabolism , Acids, Acyclic/pharmacology , Biotransformation/drug effects , Clostridium/drug effects , Disaccharides/pharmacology , Drug Synergism , Monosaccharides/pharmacologyABSTRACT
The G protein-coupled receptor 40 (GPR40)/free fatty acid receptor 1 (FFA1) has emerged as an attractive target for a novel insulin secretagogue with glucose dependency. We previously identified phenylpropanoic acid derivative 1 (3-{4-[(2',6'-dimethylbiphenyl-3-yl)methoxy]-2-fluorophenyl}propanoic acid) as a potent and orally available GPR40/FFA1 agonist; however, 1 exhibited high clearance and low oral bioavailability, which was likely due to its susceptibility to ß-oxidation at the phenylpropanoic acid moiety. To identify long-acting compounds, we attempted to block the metabolically labile sites at the phenylpropanoic acid moiety by introducing a fused-ring structure. Various fused-ring alkanoic acids with potent GPR40/FFA1 activities and good PK profiles were produced. Further optimizations of the lipophilic portion and the acidic moiety led to the discovery of dihydrobenzofuran derivative 53 ((6-{[4'-(2-ethoxyethoxy)-2',6'-dimethylbiphenyl-3-yl]methoxy}-2,3-dihydro-1-benzofuran-3-yl)acetic acid), which acted as a GPR40/FFA1 agonist with in vivo efficacy during an oral glucose tolerance test (OGTT) in rats with impaired glucose tolerance.
Subject(s)
Acetates/chemical synthesis , Acids, Acyclic/chemical synthesis , Benzofurans/chemical synthesis , Insulin/blood , Receptors, G-Protein-Coupled/agonists , Acetates/pharmacokinetics , Acetates/pharmacology , Acids, Acyclic/pharmacokinetics , Acids, Acyclic/pharmacology , Animals , Benzofurans/pharmacokinetics , Benzofurans/pharmacology , CHO Cells , Cricetinae , Cricetulus , Female , Glucose Intolerance/metabolism , Glucose Tolerance Test , Humans , Insulin/metabolism , Insulin Secretion , Models, Molecular , Protein Binding , Rats , Rats, Wistar , Species SpecificityABSTRACT
Shrimp farming accounts for more than 40% of the world shrimp production. Luminous vibriosis is a shrimp disease that causes major economic losses in the shrimp industry as a result of massive shrimp kills due to infection. Some farms in the South Asia use antibiotics to control Vibrio harveyi, a responsible pathogen for luminous vibriosis. However, the antibiotic-resistant strain was found recently in many shrimp farms, which makes it necessary to develop alternative pathogen control methods. Short-chain fatty acids are metabolic products of organisms, and they have been used as food preservatives for a long time. Organic acids are also commonly added in feeds in animal husbandry, but not in aquaculture. In this study, growth inhibitory effects of short-chain fatty acids, namely formic acid, acetic acid, propionic acid, and butyric acid, on V. harveyi were investigated. Among four acids, formic acid showed the strongest inhibitory effect followed by acetic acid, propionic acid, and butyric acid. The minimum inhibitory concentration (MIC) of 0.035% formic acid suppressed growth of V. harveyi. The major inhibitory mechanism seems to be the pH effect of organic acids. The effective concentration 50 (EC50) values at 96 h inoculation for all organic acids were determined to be 0.023, 0.041, 0.03, and 0.066% for formic, acetic, propionic, and butyric acid, respectively. The laboratory study results are encouraging to formulate shrimp feeds with organic acids to control vibrio infection in shrimp aquaculture farms.
Subject(s)
Acids, Acyclic/pharmacology , Penaeidae/virology , Vibrio/drug effects , Animals , Microbial Sensitivity TestsABSTRACT
Biological pesticides based on nucleopolyhedroviruses (NPVs) can provide an effective and environmentally benign alternative to synthetic chemicals. On some crops, however, the efficacy and persistence of NPVs is known to be reduced by plant specific factors. The present study investigated the efficacy of Helicoverpa armigera NPV (HearNPV) for control of H. armigera larvae, and showed that chickpea reduced the infectivity of virus occlusion bodies (OBs) exposed to the leaf surface of chickpea for at least 1 h. The degree of inactivation was greater on chickpea than that previously reported on cotton, and the mode of action is different from that of cotton. The effect was observed for larvae that consumed OBs on chickpea leaves, but it also occurred when OBs were removed after exposure to plants and inoculated onto artificial diet, indicating that inhibition was leaf surface-related and permanent. Despite their profuse exudation from trichomes on chickpea leaves and their low pH, organic acids-primarily oxalic and malic acid-caused no inhibition. When HearNPV was incubated with biochanin A and sissotrin, however, two minor constituents of chickpea leaf extracts, OB activity was reduced significantly. These two isoflavonoids increased in concentration by up to 3 times within 1 h of spraying the virus suspension onto the plants and also when spraying only the carrier, indicating induction was in response to spraying and not a specific response to the HearNPV. Although inactivation by the isoflavonoids did not account completely for the level of effect recorded on whole plants, this work constitutes evidence for a novel mechanism of NPV inactivation in legumes. Expanding the use of biological pesticides on legume crops will be dependent upon the development of suitable formulations for OBs to overcome plant secondary chemical effects.
Subject(s)
Acids, Acyclic/pharmacology , Cicer/virology , Flavonoids/pharmacology , Lepidoptera/virology , Nucleopolyhedroviruses/drug effects , Nucleopolyhedroviruses/physiology , Plant Leaves/virology , Virus Inactivation/drug effects , Acids, Acyclic/analysis , Acids, Acyclic/isolation & purification , Animals , Chemical Fractionation , Chromatography, High Pressure Liquid , Cicer/chemistry , Flavonoids/analysis , Flavonoids/isolation & purification , Larva/virology , Methanol/chemistry , Pest Control, Biological , Plant Leaves/chemistryABSTRACT
Acyclic 2'-azanucleosides with a phosphonomethoxy function in the side chain were obtained by coupling of diethyl {2-[N-(pivaloyloxymethyl)-N-(p-toluenesulfonyl)amino]ethoxymethyl}phosphonate with the pyrimidine nucleobases via the Vorbrüggen-type protocol. The compounds were evaluated in vitro for activity against a broad variety of RNA and DNA viruses.
Subject(s)
Acids, Acyclic/chemistry , Antiviral Agents , Aza Compounds , DNA Viruses/drug effects , Nucleosides/chemistry , Organophosphonates/chemistry , RNA Viruses/drug effects , Acids, Acyclic/chemical synthesis , Acids, Acyclic/pharmacology , Adenine/analogs & derivatives , Adenine/chemistry , Adenine/pharmacology , Antiviral Agents/chemical synthesis , Antiviral Agents/chemistry , Antiviral Agents/pharmacology , Aza Compounds/chemical synthesis , Aza Compounds/chemistry , Aza Compounds/pharmacology , Cidofovir , Cytosine/analogs & derivatives , Cytosine/chemistry , Cytosine/pharmacology , HeLa Cells , Humans , Molecular Structure , Nucleosides/chemical synthesis , Nucleosides/pharmacology , Organophosphonates/chemical synthesis , Organophosphonates/pharmacology , TenofovirABSTRACT
An efficient route for synthesizing novel allylic and cyclopropanoid phosphonic acid nucleoside analogues is described. The condensation of the bromine derivatives 6 and 18 with nucleoside bases (A, U, T, C, G) under standard nucleophilic substitution and deprotection conditions, afforded the target phosphonic acid nucleoside analogues. These compounds were evaluated for their antiviral properties against various viruses. Cyclopropanoid phosphonic adenine nucleoside analogue 23 showed significant anti-HIV activity.
Subject(s)
Acids, Acyclic/chemical synthesis , Antiviral Agents/chemical synthesis , Nucleosides/chemical synthesis , Organophosphonates/chemical synthesis , Acids, Acyclic/chemistry , Acids, Acyclic/pharmacology , Antiviral Agents/chemistry , Antiviral Agents/pharmacology , Cell Line , Drug Design , Enterovirus B, Human/drug effects , HIV-1/drug effects , Herpesvirus 1, Human/drug effects , Humans , Nucleosides/chemistry , Nucleosides/pharmacology , Organophosphonates/chemistry , Organophosphonates/pharmacologyABSTRACT
The aim of this study was to investigate the putative influence of some pharmacological agents and drugs of abuse upon the apical uptake of butyrate (BT) into Caco-2 cells. The apical uptake of (14)C-BT by Caco-2 cells was (1) time and concentration dependent, (2) pH dependent, (3) Na(+) independent and Cl(-) dependent, (4) energy dependent, (5) inhibited by several BT structural analogues (acetate, propionate, alpha-ketobutyrate, pyruvate, lactate), (6) insensitive to the anion exchange inhibitors DIDS and SITS and (7) inhibited by the monocarboxylate transport (MCT) inhibitors NPPB and pCMB. These characteristics are compatible with an involvement of MCT1-mediated transport. Acutely, uptake of a low concentration of (14)C-BT (10 microM) was reduced by acetaldehyde, acetylsalicylic acid, indomethacin, caffeine and theophylline and increased by MDMA. Chronically, uptake was increased by caffeine and decreased by tetrahydrocannabinol and MDMA; reverse transcription quantitative real-time PCR analysis showed that these three compounds decreased the mRNA levels of MCT1. Acutely, acetaldehyde, indomethacin and MDMA reduced the uptake of a high concentration of (14)C-BT (20 mM), and acetylsalicylic acid increased it. Chronically, none of the compounds affected uptake. Acetaldehyde, indomethacin and propionate seem to be competitive inhibitors of (14)C-BT uptake. Acetylsalicylic acid simultaneously increased the K (m) and the V (max) of (14)C-BT uptake. In conclusion, MCT1-mediated transport of (14)C-BT in Caco-2 cells is modulated by either acute or chronic exposure to some pharmacological agents and drugs of abuse (acetaldehyde, acetylsalicylic acid, indomethacin, caffeine, theophylline and the drugs of abuse tetrahydrocannabinol and MDMA).
Subject(s)
Butyrates/metabolism , 2,4-Dinitrophenol/pharmacology , 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology , 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/pharmacology , Acetaldehyde/pharmacology , Acids, Acyclic/pharmacology , Aspirin/pharmacology , Biological Transport, Active/drug effects , Caco-2 Cells , Caffeine/pharmacology , Cell Survival/drug effects , Dronabinol/pharmacology , Gene Expression/drug effects , Gene Expression/genetics , Humans , Hydrogen-Ion Concentration , Indomethacin/pharmacology , Ions/pharmacology , Kinetics , Monocarboxylic Acid Transporters/antagonists & inhibitors , Monocarboxylic Acid Transporters/genetics , N-Methyl-3,4-methylenedioxyamphetamine/pharmacology , Sodium Azide/pharmacology , Symporters/antagonists & inhibitors , Symporters/genetics , Theophylline/pharmacologyABSTRACT
BACKGROUND/AIMS: The effects of short-chain fatty acids and glutamine on diseased colonic mucosa, such as in inflammatory bowel disease, are not well described. The aim of this study was to investigate the role of L-glutamine and short-chain fatty acids, both via enema and oral administration, on mucosal healing in experimental colitis. METHODS: Colitis was induced with trinitrobenzenesulphonic acid in ethanol enema in rats. Saline enema (Colitis group, n: 12), L-glutamine enema (n: 12), short-chain fatty acids enema (n: 12), oral L-glutamine (n:11) and oral short-chain fatty acids (n.11) were applied twice daily for 10 days after induction of colitis. The sham group (n: 12) received only saline enema. Rats were sacrificed at the tenth day. Crypt depth and DNA content were measured in colonic mucosa. RESULTS: Crypt depth was significantly greater in both glutamine groups and short-chain fatty acids enema group than in sham and colitis groups (p<0.05). The mucosal DNA contents of the colitis and glutamine enema groups were significantly greater than both short-chain fatty acids groups (p<0.05). DNA content in the oral glutamine group was significantly greater than in the short-chain fatty acids enema group (p<0.05). CONCLUSIONS: L-glutamine enema can accelerate mucosal healing and regeneration in experimentally induced colitis in rats. When compared to glutamine in this study, short-chain fatty acids showed no beneficial effect on colitis.
Subject(s)
Colitis/drug therapy , Glutamine/pharmacology , Intestinal Mucosa/drug effects , Wound Healing/drug effects , Acids, Acyclic/pharmacology , Administration, Oral , Animals , Colitis/pathology , DNA/drug effects , Disease Models, Animal , Enema , Rats , Rats, Wistar , Regeneration/drug effectsABSTRACT
AIMS: To investigate whether treatment of animals feeds with organic acids/formaldehyde may mask the presence of Salmonella, when assessed by standard cultural methods. METHODS AND RESULTS: Four commercial treatments were applied at the manufacturers' recommended rates on feeds artificially inoculated with Salmonella. The recovery of Salmonella from these treated feeds was assessed after specific antagonists were added to the treatments during culture. A control group of treated feed received no antagonist. Masking of Salmonella was demonstrated when the addition of antagonists resulted in recovery of Salmonella from the treated feed, compared with a negative recovery when no antagonists were added. There were large variations in the efficacy of treatments, and masking was demonstrated with all four tested treatments. One formaldehyde-based product showed greater efficacy and less masking. Masking was greater when high levels of Salmonella were present in the feed. CONCLUSIONS: Some organic acid or formaldehyde-based feed treatments may mask the presence of Salmonella. SIGNIFICANCE AND IMPACT OF THE STUDY: Feeds may be deemed safe despite being contaminated with Salmonella. The use of antagonists during culture may help assess the level of Salmonella contamination when organic acid or formaldehyde treatments have been applied to feed ingredients.
Subject(s)
Acids, Acyclic/pharmacology , Animal Feed/microbiology , Food Microbiology , Formaldehyde/pharmacology , Salmonella/drug effects , Acids, Acyclic/antagonists & inhibitors , Animals , Colony Count, Microbial , Disinfectants/pharmacology , False Negative Reactions , Formaldehyde/antagonists & inhibitors , Histidine/pharmacology , Salmonella/isolation & purification , Sodium Hydroxide/pharmacology , Sorbic Acid/pharmacologyABSTRACT
In beet distilleries, condensates arising from stillage concentration could be recycled as dilution water for the fermentation step, thus preserving groundwater resources and ensuring a quality-controlled water supply. However, the recycling of condensates has been found to cause a significant reduction in fermentation activity. This study aimed to verify that condensates are toxic to alcoholic fermentation. Ten compounds found in condensates (formic, acetic, propionic, butyric, valeric, and hexanoic acids; 2,3-butanediol, furfuryl alcohol, furfural, and 2-phenyl-ethyl-alcohol) were tested. With the exception of 2,3-butanediol, they all proved to be inhibitors. At the same molar concentration, the longer the carbonaceous chain, the stronger the inhibition by fatty acids. An experimental design was used to study the inhibitory characteristics of the 10 compounds at the concentrations found in condensates. Synergistic effects were also confirmed. In real effluents, acetic acid was so highly concentrated that it became the strongest inhibitor. It is therefore necessary to eliminate it before recycling, as well as less concentrated compounds that may accumulate, as illustrated by the simulation.
Subject(s)
Alcohols/metabolism , Conservation of Natural Resources , Fermentation , Food Microbiology , Acids, Acyclic/pharmacology , Butylene Glycols/pharmacology , Fatty Acids/pharmacology , Furaldehyde/pharmacology , Furans/pharmacology , Phenylethyl Alcohol/pharmacology , Time FactorsABSTRACT
This article studies the operation of a new process for the production of biopolymers (polyhydroxyalkanoates, PHAs) at different applied organic load rates (OLRs). The process is based on the aerobic enrichment of activated sludge to obtain mixed cultures able to store PHAs at high rates and yields. A mixture of acetic, lactic, and propionic acids at different concentrations (in the range 8.5-31.25 gCOD/L) was fed every 2 h in a sequencing batch reactor (SBR). The resulting applied OLR was in the range 8.5-31.25 gCOD/L/day. Even though, as expected, the increase in the OLR caused an increase in biomass concentration (up to about 8.7 g COD/L), it also caused a relevant decrease of maximal polymer production rate. This decrease in polymer production rate was related to the different extent of "feast and famine" conditions, as function of the applied OLR and of the start-up conditions. As a consequence the best performance of the process was obtained at an intermediate OLR (20 gCOD/L/day) where both biomass productivity and PHA storage were high enough. However, at this high OLR the process was unstable and sudden decrease of performance was also observed. The sludge characterized by the highest PHA storage response was investigated by 16S rDNA clone library. The clone library contained sequences mostly from PHA producers (e.g., Alcaligenes and Comamonas genera); however many genera and among them, one of the dominant (Thauera), were never described before in relation to PHA storage response.
Subject(s)
Acids, Acyclic/metabolism , Bacteria/growth & development , Biopolymers/biosynthesis , Bioreactors/microbiology , Polyesters , Waste Disposal, Fluid , Acids, Acyclic/pharmacology , Biomass , Water PurificationABSTRACT
Organic acids play an important role in metal uptake by, and accumulation in, plants. However, the relevant mechanisms remain obscure. Acetic, malic and citric acids increased the uptake of lanthanum (La) by barley (Hordeum vulgare) roots and enhanced La content in shoots under hydroponic conditions. Concentration-dependent net La influx in the absence and presence of organic acids yielded nonsaturating kinetic curves that could be resolved into linear and saturable components. The saturable component followed Michaelis-Menten kinetics. The K(m) values were similar; however, the V(max) values in the presence of acetic, malic and citric acids were 4.3, 2.8, 1.5-times that of the control, respectively. Enhanced uptake of La by organic acids was mediated mainly, but not solely, by Ca(2+) channels. X-ray absorption spectroscopic techniques provided evidence of La-oxygen environment and established that La(III) was coordinated to 11 oxygen atoms that are likely to be involved in the binding of La(III) to barley roots via carboxylate groups and hydration of La(III).
Subject(s)
Acids, Acyclic/pharmacology , Hordeum/drug effects , Hordeum/metabolism , Lanthanum/metabolism , Plant Roots/drug effects , Plant Roots/metabolism , Acetic Acid/pharmacology , Citric Acid/pharmacology , Ion Channels/metabolism , Malates/pharmacologyABSTRACT
In previous work, we showed that the binding of the liver x receptor alpha:peroxisome proliferator-activated receptor alpha (LXRalpha:PPARalpha) heterodimer to the murine Cyp7a1 gene promoter antagonizes the stimulatory effect of their respective ligands. In this study, we determined if LXRalpha:PPARalpha can also regulate human CYP7A1 gene promoter activity. Co-expression of LXRalpha and PPARalpha in McArdle RH7777 hepatoma cells decreased the activity of the human CYP7A1 gene promoter in response to fibrates and 25-hydroxycholesterol. In vitro, the human CYP7A1 Site I bound LXRalpha:PPARalpha, although with substantially less affinity compared with the murine Cyp7a1 Site I. The binding of LXRalpha:PPARalpha to human CYP7A1 Site I was increased in the presence of either LXRalpha or PPARalpha ligands. In HepG2 hepatoblastoma cells, fibrates and 25-hydroxycholesterol inhibited the expression of the endogenous CYP7A1 gene as well as the human CYP7A1 gene promoter when co-transfected with plasmids encoding LXRalpha and PPARalpha. However, a derivative of the human CYP7A1 gene promoter that contains a mutant form of Site I that does not bind LXRalpha:PPARalpha was not inhibited by WY 14,643 or 25-hydroxycholesterol in both McArdle RH7777 and HepG2 cells. The ligand-dependent recruitment of LXRalpha:PPARalpha heterodimer onto the human CYP7A1 Site I can explain the inhibition of the human CYP7A1 gene promoter in response to fibrates and 25-hydroxycholesterol.