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Bioanalysis ; 7(2): 207-20, 2015.
Article in English | MEDLINE | ID: mdl-25587837

ABSTRACT

BACKGROUND: In this study, we developed and validated a HPLC-MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. RESULTS & METHODOLOGY: Chromatographic separation was achieved using a C8 column with a mobile phase consisting of a gradient of water and acetonitrile:methanol (90:10 v/v), both containing 0.1% formic acid. The developed method was selective, sensitive (LD<7.0 ng ml(-1)), linear (r>0.99), precise (RSD<11.3%), accurate (RE<11.8%) and free of residual and matrix effects. The developed method was successfully applied in plasma patients with Parkinson's disease using Stalevo®. CONCLUSION: The new method can be used for the clinical monitoring of these substances and applied to adjustments in drug dosages.


Subject(s)
Benzophenones/blood , Blood Chemical Analysis/methods , Carbidopa/blood , Catechols/blood , Chromatography, High Pressure Liquid , Dihydroxyphenylalanine/analogs & derivatives , Dopamine/blood , Levodopa/blood , Nitriles/blood , Nitrophenols/blood , Tandem Mass Spectrometry , Benzophenones/standards , Carbidopa/standards , Catechols/standards , Chromatography, High Pressure Liquid/standards , Dihydroxyphenylalanine/blood , Dihydroxyphenylalanine/standards , Dopamine/standards , Humans , Levodopa/standards , Nitriles/standards , Nitrophenols/standards , Quality Control , Reproducibility of Results , Tandem Mass Spectrometry/standards , Tolcapone , Tyrosine/analogs & derivatives
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