RESUMEN
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
Asunto(s)
Infecciones por Actinomycetales/veterinaria , Actinomycetales/aislamiento & purificación , Enfermedades de los Bovinos/diagnóstico , Colorantes Fluorescentes , Enfermedades de los Caballos/diagnóstico , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Enfermedades de las Ovejas/diagnóstico , Infecciones por Actinomycetales/diagnóstico , Infecciones por Actinomycetales/microbiología , Animales , Bovinos , Enfermedades de los Bovinos/microbiología , Enfermedades de los Caballos/microbiología , Caballos , Límite de Detección , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria , Reproducibilidad de los Resultados , Ovinos , Enfermedades de las Ovejas/microbiologíaRESUMEN
The current study describes the recovery of Helcococcus ovis from an adult female Verata breed goat that was euthanized because of respiratory problems and emaciation. At necropsy, the affected animal showed a purulent bronchopneumonia with scattered pulmonary abscesses and fibrous pleural adhesions. A Gram-positive, catalase-negative, coccus-shaped bacterium was isolated from the lung tissue and confirmed as H. ovis by 16S ribosomal RNA gene sequencing.