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INTRODUCTION: The diagnosis of groin hernia is based on clinical symptoms and physical examination. In the case of equivocal clinical findings, patients are often referred for subsequent diagnostic imaging. Accurate detection is important to minimize the inherent risk of complications or to avoid unnecessary surgery. Although herniography has been reported as a save and highly accurate procedure, it has not gained widespread acceptance in the diagnostic work-up of groin hernias. METHODS: We retrospectively analysed 157 patients who underwent herniography in our department, which is to date the third largest study reporting on this technique. The diagnostic value of herniography was investigated--with laparascopic surgical findings serving as a gold standard--in comparison to clinical symptoms, physical examination and ultrasound. RESULTS: Herniography showed a substantial agreement with the surgical findings, but only a slight to fair agreement was found between surgery and clinical symptoms and examination. Poor agreement was found between sonographic and surgical findings. CONCLUSION: Based on the presented data and previously reported studies, we can conclude that herniography is a safe technique with a high accuracy to detect groin hernias in patients with equivocal clinical presentation, whereas ultrasound has a wide range in reported sensitivity. Clinicians and surgeons should take this into account when referring patients for subsequent imaging.
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Medios de Contraste/administración & dosificación , Hernia Inguinal/diagnóstico por imagen , Adulto , Anciano , Femenino , Hernia Inguinal/cirugía , Humanos , Laparoscopía , Masculino , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Periodo Preoperatorio , Radiografía , Estudios Retrospectivos , Sensibilidad y Especificidad , Resultado del Tratamiento , UltrasonografíaRESUMEN
OBJECTIVE: The peri-operative and immediate post-operative outcome of secondary hyperparathyroidism treated with subtotal parathyroidectomy is reported. METHODS: We studied 100 patients with chronic renal failure who underwent subtotal parathyroidectomy at our department. Surgical eligibility was based on hyperparathyroidism stage, defined by symptoms of osteodystrophy and/or the presence of hypercalcemia and hyperphosphatemia refractory to medical treatment. Parathormone levels were measured pre-operatively and during the first post-operative days. RESULTS: During surgery, four parathyroid glands were identified in 86% of patients, five glands in 1%, and less than four glands in 13%. The ratio of hyperplastic to normal glands was 93:7. No correlation was found between anatomic location of the glands and the presence of hyperplasia. Parathormone decreased to normal or very low values in 93% of the patients. In seven cases, the lowest post-operative parathormone value was above 30 pg/ml, although four glands were removed in four of these patients. In 95% of the patients with four or more identified glands, post-operative serum parathormone levels decreased to normal or very low values. In 23% of the patients with less than four glands, parathormone levels remained too high. On the other hand, post-operative parathormone values normalized in 10 patients who had less than four glands identified during surgery; in two of them, parathyroid tissue was found during postoperative pathological examinations of the resected thyroid lobe. CONCLUSIONS: Subtotal parathyroidectomy is an acceptable treatment in patients with refractory hyperparathyroidism. Our results indicate that there was not a perfect correlation between the number of identified glands and post-operative parathormone in a subset of patients.
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Hiperparatiroidismo Secundario/cirugía , Hormona Paratiroidea/sangre , Paratiroidectomía , Adulto , Anciano , Anciano de 80 o más Años , Humanos , Hiperparatiroidismo Secundario/etiología , Fallo Renal Crónico/complicaciones , Persona de Mediana Edad , Periodo Posoperatorio , Adulto JovenRESUMEN
OBJECTIVE: In cases of re-operation for secondary hyperparathyroidism, to evaluate the extent to which the location of recurrent hyperplasia was predicted by (1) operative data from the first intervention, and (2) pre-operative imaging (before the re-operation). METHODS: The files of 18 patients undergoing surgery for recurrent secondary hyperparathyroidism were reviewed. The surgical findings were compared both with the report of the initial operation and with the results of pre-operative imaging (i.e. ultrasonography, Mibi scintigraphy or computed tomography). RESULTS: The location of the recurrent hyperplasia corresponded with the data for the primary intervention in about one-third of patients. There was a partial correlation in one-third of patients, and no correlation at all in one-third. Pre-operative imaging enabled better prediction of the location of recurrent disease. CONCLUSION: Surgeons should have both sources of information at their disposal when planning a re-intervention for secondary hyperparathyroidism. However, in our series, the predictive value of imaging was superior to that of information deduced from the previous surgical record.
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Diagnóstico por Imagen/normas , Hiperparatiroidismo Secundario/diagnóstico , Glándulas Paratiroides/patología , Adolescente , Adulto , Anciano , Diagnóstico por Imagen/métodos , Femenino , Humanos , Hiperparatiroidismo Secundario/cirugía , Hiperplasia/diagnóstico , Hiperplasia/cirugía , Masculino , Persona de Mediana Edad , Glándulas Paratiroides/cirugía , Valor Predictivo de las Pruebas , Recurrencia , Reoperación , Sensibilidad y EspecificidadRESUMEN
The authors suggest some criteria by which pseudodystrophy and reflex sympathetic dystrophy, although sharing some similar clinical features, can be distinguished as two different conditions, each requiring its own approach and management. The most important distinction is found on bone scintigraphy. In reflex sympathetic dystrophy the bone scan shows a typical increased tracer uptake (at least during stages I and II); in pseudodystrophy there is a normal or decreased tracer uptake in the affected region. Moreover the vascularization is increased in reflex sympathetic dystrophy stage I, whereas in pseudodystrophy hypovascularization is found from the beginning. The clinical features, as well as the results of technical investigations, psychological evaluation and treatment of 4 patients with pseudodystrophy are presented. The importance of distinguishing this condition from reflex sympathetic dystrophy is stressed.
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Trastornos de Conversión/diagnóstico , Distrofia Simpática Refleja/diagnóstico , Adolescente , Adulto , Trastornos de Conversión/diagnóstico por imagen , Trastornos de Conversión/terapia , Diagnóstico Diferencial , Femenino , Humanos , Trastornos Mentales/complicaciones , Trastornos Mentales/diagnóstico , Dolor/etiología , Dolor/psicología , Cintigrafía , Distrofia Simpática Refleja/diagnóstico por imagen , Distrofia Simpática Refleja/terapia , Síndrome , Vasodilatadores/uso terapéuticoRESUMEN
A significant increase in airway responsiveness to histamine was observed in vitro and in vivo 4 days after intratracheal inoculation of parainfluenza Type 3 (PI-3) virus to guinea pigs. Light microscopic and ultrastructural examination of the central airways of animals inoculated with virus revealed stratification of the epithelial lining, with pronounced loss of cilia and granule-depleted goblet cells. In the peripheral airways, typical lesions of patchy alveolitis and bronchiolitis were found. The alveolar epithelium often lacked Type I alveolar cells and was lined merely by cells containing osmiophilic lamellar bodies typical of Type II alveolar cells. PI-3 virus inoculation resulted in a reduction in the number of airway mucosal mast cells, particularly in the bronchioles, and in a change of density of the granules of mast cells. Further, a significant rise (100%) in histamine concentration was observed in lung lavage fluid after virus inoculation. The prostaglandin E2 (PGE2) content in the lavage fluid was not changed. After stimulation with histamine, the tracheae of animals inoculated with control solution or PI-3 virus produced similar amounts of PGE2. These data indicate that PI-3 virus activates airway mast cells and increases the histamine content in the respiratory tract. Neither the virus-induced lung damage nor the increased levels of histamine in the airways influence the release of the epithelially derived relaxing factor PGE2. It is suggested that mast cell-derived products, in particular histamine, are involved in virus-induced airway hyperresponsiveness.
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Hiperreactividad Bronquial/etiología , Histamina/metabolismo , Pulmón/patología , Virus de la Parainfluenza 3 Humana , Infecciones por Paramyxoviridae/complicaciones , Análisis de Varianza , Animales , Hiperreactividad Bronquial/epidemiología , Hiperreactividad Bronquial/metabolismo , Hiperreactividad Bronquial/patología , Líquido del Lavado Bronquioalveolar/química , Líquido del Lavado Bronquioalveolar/citología , Dinoprostona/metabolismo , Relación Dosis-Respuesta a Droga , Cobayas , Histamina/farmacología , Técnicas In Vitro , Pulmón/efectos de los fármacos , Masculino , Mastocitos/efectos de los fármacos , Mastocitos/metabolismo , Mastocitos/patología , Infecciones por Paramyxoviridae/epidemiología , Infecciones por Paramyxoviridae/metabolismo , Infecciones por Paramyxoviridae/patología , Organismos Libres de Patógenos Específicos , Tráquea/efectos de los fármacos , Tráquea/patologíaRESUMEN
Microtubule (MT) dynamics in PtK2 cells have been investigated using in vivo injection of unmodified Paramecium ciliary tubulin and time-lapse fixation. The sites of incorporation of the axonemal tubulin were localized using a specific antibody which does not react with vertebrate cytoplasmic tubulin (Adoutte, A., M. Claisse, R. Maunoury, and J. Beisson. 1985. J. Mol. Evol. 22:220-229), followed by immunogold labeling, Nanovid microscopy, and ultrastructural observation of the same cells. We confirm data from microinjection of labeled tubulins in other cell types (Soltys, B. J., and G. G. Borisy. 1985. J. Cell Biol. 100:1682-1689; Mitchison, T., L. Evans, E. Schulze, and M. Kirschner. 1986. Cell. 45:515-527; Schulze, E., and M. Kirschner. 1986. J. Cell Biol. 102:1020-1031). In agreement with the dynamic instability model (Mitchison, T., and M. Kirschner. 1984. Nature (Lond.). 312:237-242), during interphase, fast (2.6 microns/min) distal growth of MTs occurs, together with new centrosomal nucleation. Most of the cytoplasmic MT complex is replaced within 15-30 min. During mitosis, astral MTs display the same pattern of renewal, but the turnover of the MT system is much faster (approximately 6 min). We have concentrated on the construction of the kinetochore fibers during prometaphase and observe that (a) incorporation of tubulin in the vicinity of the kinetochores is not seen during prophase and early prometaphase as long as the kinetochores are not yet connected to a pole by MTs; (b) proximal time-dependent incorporation occurs only into preexisting kinetochore MTs emanating from centrosomes. Consequently, in undisturbed prometaphase cells, the kinetochores probably do not act as independent nucleation sites. This confirms a model in which, at prometaphase, fast probing centrosomal MTs are grabbed by the kinetochores, where tubulin incorporation then takes place.
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Centrómero/metabolismo , Cromosomas/metabolismo , Metafase , Microtúbulos/metabolismo , Tubulina (Proteína)/metabolismo , Animales , Línea Celular , Centrómero/ultraestructura , Técnica del Anticuerpo Fluorescente , Inmunohistoquímica , Interfase , Microinyecciones , Microscopía Electrónica , Microtúbulos/ultraestructura , Paramecium , Profase , Huso Acromático/ultraestructuraRESUMEN
Colchicine and related drugs are known to inhibit milk secretion. They are also able to prevent stimulation of casein and DNA synthesis by prolactin in the mammary gland. The present report reports data obtained with tubulozole, a new antimitotic drug. Tubulozole C added to culture medium of isolated rabbit epithelial mammary cells strongly inhibited their multiplication. Simultaneously, at a concentration of 1 microM, it prevented almost completely the induction of beta-casein mRNA. Induced cells were rapidly deinduced by addition of the drug to the medium. A similar inhibition was observed when the induction was obtained with prolactin alone or with its two stimulators insulin and glucocorticoids. Tubulozole T, an isomer of tubulozole C which is known to be ineffective in disrupting microtubules, did not alter prolactin actions. These data and those obtained with other tubulin-binding drugs strongly suggest that the integrity of microtubules is required for prolactin to deliver its message to the mammary cell.
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Antineoplásicos/farmacología , Caseínas/genética , Dioxolanos/farmacología , Dioxoles/farmacología , Genes/efectos de los fármacos , Glándulas Mamarias Animales/metabolismo , Microtúbulos/ultraestructura , Prolactina/farmacología , Transcripción Genética/efectos de los fármacos , Animales , Bencimidazoles/farmacología , Células Cultivadas , Colchicina/farmacología , Femenino , Técnica del Anticuerpo Fluorescente , Glándulas Mamarias Animales/efectos de los fármacos , Microtúbulos/efectos de los fármacos , Nocodazol , Embarazo , ARN Mensajero/genética , ConejosRESUMEN
Immunofluorescence with specific peptide antibodies has previously established that tyrosinated (Tyr) and detyrosinated (Glu) tubulin, the two species generated by posttranslational modification of the COOH-terminus of alpha-tubulin, are present in distinct, but overlapping, subsets of microtubules in cultured cells (Gundersen, G. G., M. H. Kalnoski, and J. C. Bulinski, 1984, Cell, 38:779-789). Similar results were observed by light microscopic immunogold staining in the two cell types used in this study, CV1 and PtK2 cells: most microtubules were stained with the Tyr antibody, whereas only a few were stained with the Glu antibody. We have examined immunogold-stained preparations by electron microscopy to extend these results. In general, electron microscopic localization confirmed results obtained at the light microscopic level: the majority of the microtubules in CV1 and PtK2 cells were nearly continuously labeled with the Tyr antibody, whereas only a few were heavily labeled with the Glu antibody. However, in contrast to the light microscopic staining, we found that all microtubules of interphase and mitotic CV1 and PtK2 cells contained detectable Tyr and Glu immunoreactivity at the electron microscopic level. No specific localization of either species was observed in microtubules near particular organelles (e.g., mitochondria or intermediate filaments). Quantification of the relative levels of Glu and Tyr immunoreactivity in individual interphase and metaphase microtubules showed that all classes of spindle microtubules (i.e., kinetochore, polar, and astral) contained nearly the same level of Glu immunoreactivity; this level of Glu immunoreactivity was lower than that found in all interphase microtubules. Most interphase microtubules had low levels of Glu immunoreactivity, whereas a few had relatively high levels; the latter corresponded to morphologically sinuous microtubules. Quantification of the relative levels of Tyr and Glu immunoreactivity in segments along individual microtubules suggested that the level of Tyr (or Glu) tubulin in a given microtubule was uniform along its length. Understanding how microtubules with different levels of Tyr and Glu tubulin arise will be important for understanding the role of tyrosination/detyrosination in microtubule function. Additionally, the coexistence of microtubules with different levels of the two species may have important implications for microtubule dynamics in vivo.
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Microtúbulos/ultraestructura , Tubulina (Proteína)/metabolismo , Tirosina/metabolismo , Animales , Línea Celular , Chlorocebus aethiops , Dipodomys , Oro , Interfase , Microscopía Electrónica/métodos , Procesamiento Proteico-Postraduccional , Huso Acromático/ultraestructuraAsunto(s)
Alcaloides/farmacología , Bencimidazoles/farmacología , Ciclo Celular/efectos de los fármacos , Microtúbulos/ultraestructura , Mitosis/efectos de los fármacos , Animales , Línea Celular , Interfase/efectos de los fármacos , Riñón , Marsupiales , Metafase/efectos de los fármacos , Microscopía Electrónica , Microtúbulos/efectos de los fármacos , Películas Cinematográficas , Nocodazol , Paclitaxel , Profase/efectos de los fármacosRESUMEN
We describe a new approach to probe the molecular biology of the living cell that uses small colloidal gold particles coupled to specific ligands. They are visualized in cells by bright-field, video enhanced contrast microscopy. We describe the basic aspects of the technique and provide examples of applications to intracellular motility, cell membrane dynamics, receptor translocation, internalization, and intracellular routing. We also provide examples of the use of this approach in immunospecific labelling of cells and tissue sections.
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Células/ultraestructura , Oro , Animales , Transporte Biológico , Línea Celular , Membrana Celular/fisiología , Citoplasma/fisiología , Citoplasma/ultraestructura , Histocitoquímica , Humanos , Técnicas Inmunológicas , Ligandos , Microinyecciones , Microscopía , Microscopía Electrónica , Microtúbulos/fisiología , Microtúbulos/ultraestructura , Movimiento , Tamaño de la Partícula , Receptores de Superficie Celular/metabolismo , Grabación en VideoAsunto(s)
Microtúbulos/fisiología , Animales , Coloides , Oro , Microscopía/métodos , Microtúbulos/ultraestructuraAsunto(s)
Antineoplásicos/farmacología , Dioxolanos/farmacología , Dioxoles/farmacología , Microtúbulos/efectos de los fármacos , Animales , Encéfalo/metabolismo , Línea Celular , Transformación Celular Neoplásica , Perros , Cinética , Ratones , Ratones Endogámicos C3H , Microtúbulos/metabolismo , Microtúbulos/ultraestructura , Tubulina (Proteína)/metabolismoRESUMEN
Light and electron microscopic investigations on mammalian cells in vitro and in vivo showed that tubulozole-C (R 46 846), the cis-isomer of tubulozole, a new synthetic anticancer drug, interfered with the structure and function of microtubules in both interphase and mitotic cells. The activity of this compound in experimental tumor systems can thus be explained partly by a direct antimitotic effect and partly by the disintegration of the normal subcellular organization of the nondividing cells. At concentrations which affect the microtubule system, tubulozole-C arrested directional migration of transformed cells and malignant invasion in a three-dimensional organ culture system. Investigations in vivo show that malignant L1210 leukemia cells are more susceptible to the antimicrotubular effect of tubulozole-C than are the normal leukocytes of the host. The trans-isomer of tubulozole (tubulozole-T, R 48 265), which has no antitumor activity in vivo, did not affect the microtubule system of cells in vitro or their capacity for directional migration or for malignant invasion.
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Dioxolanos/farmacología , Dioxoles/farmacología , Microtúbulos/efectos de los fármacos , Animales , División Celular/efectos de los fármacos , Células Cultivadas , Pollos , Dipodomys , Femenino , Humanos , Leucemia L1210/patología , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos DBA , Microscopía Electrónica , Miocardio/metabolismo , Polímeros/metabolismo , Embarazo , Tubulina (Proteína)/metabolismoRESUMEN
Colloidal gold particles of 20 to 40 nm diameter stabilized with polyethylene glycol (PEG) were microinjected in PTK2 cells. Aggregates and individual particles, which are smaller than the theoretical limit of resolution of the optical microscope and invisible to the eye are discernible from organelles by reflection of polarized light. They are optimally visualized using transmitted light and electronic subtraction of diffuse background light. The gold particles show saltatory motion. The direction, speed, median distance travelled and frequency of saltations are indiscernible from measurements made on cell organelles in the same preparations. Because microtubule treadmilling has been implicated as a potential motor for organelle motility, gold particles coupled to monoclonal antibodies, recognizing the alpha-subunit of tubulin (Kilmartin et al., 1982), were injected. These particles, often forming linear arrays, assumed entirely fixed positions in the cell. The results suggest that there is a transport system associated with microtubules which can carry synthetic particles through the cell without the need for them being covered with specific proteins. Microtubule treadmilling does not seem to be involved. The possibility of following 20-40 nm particles and probably even smaller ones, that can be coupled to most proteins, within living cells provides a tool of wide applicability to study the fate and behaviour of such proteins. It is suggested that this new method be called nanoparticle video ultramicroscopy or nanovid ultramicroscopy.
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Microscopía de Contraste de Fase/métodos , Microtúbulos/fisiología , Movimiento (Física) , Animales , Transporte Biológico Activo , Ciclo Celular , Línea Celular , Citoplasma/fisiología , Oro , Macropodidae , Microscopía de Contraste de Fase/instrumentación , Microtúbulos/ultraestructura , Peso Molecular , Tamaño de la Partícula , Grabación de Cinta de VideoRESUMEN
Microtubules and microfilaments were investigated in hamster lung fibroblasts, during their in vitro life-span. These cells show a senescence process characterized by a drastic phenotypic change, resulting in two phenotypes: the type 1 cells, characteristic of young cultures and the type 2 cells appearing progressively with culture passages. Microtubules and microfilaments were observed at the TEM and also visualized by the unlabelled peroxidase-anti-peroxidase method. Moreover, the susceptibility of microtubules to nocodazole was tested in type 1 and 2 cells. We could not provide evidence for a different susceptibility to the drug. However the depolymerization wave occurred centripetally in type 1 cells whilst centrifugally in type 2 cells. These observations are discussed in relationship with the early arrest of division growth of the type 2 differentiated cells.
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Supervivencia Celular , Células Cultivadas/ultraestructura , Citoesqueleto/ultraestructura , Microtúbulos/ultraestructura , Animales , División Celular , Cricetinae/embriología , Pulmón/embriología , Microscopía Electrónica , Microscopía FluorescenteRESUMEN
Using double-label immunofluorescence and electron microscopy we studied the interaction between microtubules (MT) and intermediate filaments (IF) in MO cells treated with various combinations of taxol and nocodazole. With taxol, the organized MT of cultured cells are replaced by free MT and MT bundles. This rearrangement of MT is followed by a rearrangement of the IF. As in untreated cells a close association between these two filamentous systems is observed. In cells pretreated with nocodazole followed by addition of taxol, to induce the bundles of free MT, the preexisting IF coils disappear and IF associate with the MT. From these experiments we conclude that an interaction between MT and IF exists independent of the normal organisation of the MT system. The redistribution of IF always follows the redistribution of MT. The data show that MT determine the spatial distribution of IF which most probably involves some kind of physicochemical link.