A fuzzy quality index for the environmental assessment of a restored wetland.

This paper describes the feasibility study for the restoration of agricultural land with a tendency to become waterlogged into a natural wetland, conceived to mitigate floods and to remove nutrients from the water drained from the cultivated plots. The wetland model, developed in aquatox, includes the nutrient dynamics both in the water and in the sediment, and the vegetation that is expected to develop as a consequence of flooding. The model inputs were synthesized from historical time series of rainfall and chemical data collected over the last decade. The model outputs are used to compute a synthetic fuzzy quality index (FQI) to assess the removal efficiency of the wetland. This FQI is based on three main variables describing the ecosystem quality: chlorophyll-a, dissolved oxygen and total suspended solids. This index has the merit of being simple enough to be immediately grasped by non-technical people, like managers and stakeholders, to whom the restoration project is proposed. The simulations, performed under five differing loading scenarios demonstrate the feasibility of this solution, which is robust enough to accommodate a 50% increase in either nitrogen, phosphorous or organic matter.

Relação entre as habilidades fonológicas e pragmáticas nos distúrbios específicos de linguagem / Relation between phonologic and pragmatic habilities in specific language impairment

BACKGROUND: Specific language impairment. AIM: To establish relations between the performance of subjects in phonologic and pragmatic tests. METHOD: Tabulation and analyses of the data of phonologic and pragmatic tests of 20 children with ages raging from 3:0 to 5:11 years, with the diagnoses of Specific Language Impairment (SLI). RESULTS: The statistic analyses showed good correlations between developmental processes (DP) and non-developmental processes (NDP), DP and the use of verbal mean, vocal mean and number of communication acts per minute. CONCLUSION: The performance in the phonological and pragmatic tests does not seem to have a direct correlation in subjects with specific language impairment.

Tema: distúrbios específicos de linguagem. Objetivo: estabelecer relações a partir do desempenho dos sujeitos nas provas de pragmática e fonologia. Método: tabulação eanálise dos dados das provas de fonologia e pragmática de 20 crianças, com idade entre 3:00 e 5:11 anos, com diagnóstico de Distúrbio Especifico de Linguagem (DEL). Resultados:a análise estatística mostrou correlações boas e regulares entre processos de desenvolvimento (PD) e processos de não desenvolvimento (PnD), PD e meio verbal, meiovocal e atos por minuto. Conclusão: o desempenho nas provas de fonologia e pragmática parece não apresentar correlação estritamente direta nos sujeitos com DEL.

Horse urinary kallikrein, I. Complete purification and characterization.

The isolation procedure for horse urinary kallikrein was considerably improved by the introduction of two new purification steps: a) removal of mucoproteins and concentration of the urine by ultrafiltration and b) affinity chromatography on benzamidine-Sepharose conjugate. The homogeneity of the enzyme preparations, regarding their protein moiety, was demonstrated by: 1) a single symmetric peak on DEAE-Sephadex chromatography, with constant values for A280/A260 ratios, esterolytic and amidolytic specific activities; 2) a single band, although dispersed, on gel-electrophoresis at pH 8.3, also in the presence of sodium dodecyl sulfate, and 3) a unique sequence for the six amino-terminal residues. The isolated enzyme was shown to be a single chain glycoprotein (alpha-kallikrein), similar to human urinary and porcine-pancreatic kallikreins regarding the protein moiety molecular mass, amino-acid composition, and partial amino-terminal sequence; differences were found in their total sugar content and even more conspicuously in their carbohydrate composition. In contrast to porcine pancreatic beta-kallikrein, horse urinary kallikrein was not substrate-activated and unlike other alpha-kallikreins, did not present the biphasic time-course in benzoyl-L-arginine ethyl ester hydrolysis. The specificity constants (kcat/Km) for ester and 4-nitroanilide substrates were lower for horse urinary than for pancreatic beta-kallikrein and as observed with the latter enzyme, were affected by NaCl.