RESUMEN
Cks1 is an essential factor in facilitating Skp2-dependent degradation of p27, but its role in salivary malignancies is unknown. Expression of cyclin-dependent kinase subunit 1 (Cks1) was examined in 64 salivary malignancies, compared with p27, S-phase kinase protein 2 (Skp2), Ki-67, p53, and TDT-mediated dutp-biotin nick end labeling (TUNEL) expression, and with THE patient's clinical and pathological parameters. Cks1 expression was markedly increased in 30 patients (47%) and strongly correlated with increased expression of Skp2, Ki-67, p53, and TUNEL, but inversely with p27 levels. High expression of Cks1 WAS strongly associated with lymph node metastases and poor prognosis and survival. Cks1 alterations may have a significant biological role in the pathogenesis of salivary cancer.
Asunto(s)
Proteínas Portadoras/metabolismo , Quinasas Ciclina-Dependientes/metabolismo , Neoplasias de las Glándulas Salivales/enzimología , Adenocarcinoma/enzimología , Adenocarcinoma/secundario , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Biomarcadores de Tumor/metabolismo , Quinasas CDC2-CDC28 , Carcinoma de Células Acinares/enzimología , Carcinoma de Células Acinares/secundario , Carcinoma Mucoepidermoide/enzimología , Carcinoma Mucoepidermoide/secundario , Carcinoma de Células Escamosas/enzimología , Carcinoma de Células Escamosas/secundario , Proteínas Portadoras/genética , Quinasas Ciclina-Dependientes/genética , Femenino , Humanos , Técnicas para Inmunoenzimas , Etiquetado Corte-Fin in Situ , Antígeno Ki-67/metabolismo , Metástasis Linfática , Masculino , Persona de Mediana Edad , Pronóstico , Antígeno Nuclear de Célula en Proliferación/metabolismo , Proteínas Quinasas Asociadas a Fase-S/metabolismo , Neoplasias de las Glándulas Salivales/patología , Tasa de Supervivencia , Proteína p53 Supresora de Tumor/metabolismo , Adulto JovenRESUMEN
OBJECTIVE: To draw attention to a rare, life threatening complication of a rather common procedure, namely medullary injury following adenoidectomy and local anesthetic infiltration of the operative bed. DESIGN: Case report. SETTING: A tertiary pediatric critical care unit. PATIENT: A healthy 7-year-old girl underwent adenoidectomy and local anesthetic infiltration of the adenoid bed with lidocaine and adrenaline. In the recovery room, nystagmus, dysarthria, dyspnea, inability to cough and right hemiparesis were noticed. Because of her inability to remove secretions tracheal intubation was performed, followed by severe, life threatening respiratory failure. INTERVENTIONS: Tracheal intubation, hemodynamic support, prolonged mechanical ventilation, nitric oxide, and tracheostomy. CONCLUSION: In children, local anesthetic infiltration of the adenoid bed may cause life-threatening medullary injury and its routine use should be re-considered.